Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	774,615	C→G	100%	intergenic (‑69/+51)	oppB1 ← / ← oppA1	oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	774,615	0	C→G	100.0%	27.0	7	intergenic (‑69/+51)	oppB1/oppA1	oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA
Reads supporting (aligned to +/- strand):  new base (7/0):  ref base (0/0):  total (7/0)

GGGCCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTG.CCGGT.CG.A.CGTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCG  >  NC_014034/774597‑774705                   |                                                                                               catcaacggcgcCCCCCCGCCCGGGTCGGACCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGtt            >  2:3061707/9‑101 (MQ=255) caggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCCCGtt            >  2:284403/9‑101 (MQ=255)  aggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTc           >  2:3133495/8‑101 (MQ=255)    gcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCa         >  2:3819158/6‑101 (MQ=255)        ggcgcCCCCCCGCCCGGGTCGGACCGGCCCTG.CCGGTCCG.ACCCCACGGTATCTTATCCTGCGATCACTTCGTCCGCGACCAGTCGGCAACGTTCCAGTAt     >  2:478285/2‑101 (MQ=255)        ggcgcCCCCCCGCCCGGGGCGGACCGGCCCCGCCCGGC.CGCC.CCCACGGTCTCTTATCCTGCGATCACTTCGCCCCCGACCCGGCGGCCACGTTcccggat     >  1:390461/2‑96 (MQ=255)           gcCCCCCCGCCCGGGTCGGACGGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCCCGTTCCCGGATTCg  >  1:2650545/1‑101 (MQ=255)                   |                                                                                               GGGCCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTG.CCGGT.CG.A.CGTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCG  >  NC_014034/774597‑774705 Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG