Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	774,645	G→C	100%	intergenic (‑99/+21)	oppB1 ← / ← oppA1	oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	774,645	0	G→C	100.0%	96.8	25	intergenic (‑99/+21)	oppB1/oppA1	oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA
Reads supporting (aligned to +/- strand):  new base (23/2):  ref base (0/0):  total (23/2)

GGGCCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTG.CCGGT.CG.A.CGTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAC  >  NC_014034/774597‑774728                                                     |                                                                                    catcaacggcgcCCCCCCGCCCGGGTCGGACCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGtt                                   >  2:3061707/9‑101 (MQ=255) caggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCCCGtt                                   >  2:284403/9‑101 (MQ=255)  aggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTc                                  >  2:3133495/8‑101 (MQ=255)    gcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCa                                >  2:3819158/6‑101 (MQ=255)        ggcgcCCCCCCGCCCGGGTCGGACCGGCCCTG.CCGGTCCG.ACCCCACGGTATCTTATCCTGCGATCACTTCGTCCGCGACCAGTCGGCAACGTTCCAGTAt                            >  2:478285/2‑101 (MQ=255)        ggcgcCCCCCCGCCCGGGGCGGACCGGCCCCGCCCGGC.CGCC.CCCACGGTCTCTTATCCTGCGATCACTTCGCCCCCGACCCGGCGGCCACGTTcccggat                            >  1:390461/2‑96 (MQ=255)           gcCCCCCCGCCCGGGTCGGACGGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCCCGTTCCCGGATTCg                         >  1:2650545/1‑101 (MQ=255)                     ccgggtcggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAgg               >  2:1796092/10‑101 (MQ=255)                      cgggtcggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGc              >  1:1798786/9‑101 (MQ=255)                       gggtcggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCg             >  2:2565656/8‑101 (MQ=255)                       gggtcggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCg             <  1:4166934/94‑1 (MQ=255)                        ggtcggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGt            >  1:2311324/7‑101 (MQ=255)                        ggtcggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGt            >  1:802062/7‑101 (MQ=255)                          tcggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTc          >  2:3307971/5‑101 (MQ=255)                           cggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCNCCTNCCAGTATTCGCNGTCCCAGGCGTTCa         >  1:4266375/4‑101 (MQ=255)                           cggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa         >  2:265685/4‑101 (MQ=255)                           cggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa         >  1:999618/4‑101 (MQ=255)                           cggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa         >  1:474176/4‑101 (MQ=255)                           cggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa         >  1:1628304/4‑101 (MQ=255)                            ggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAt        >  2:968851/3‑101 (MQ=255)                             gaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtc       >  2:3677160/2‑101 (MQ=255)                             gaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtc       >  1:2049142/2‑101 (MQ=255)                               ccGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTGCCAGTATTCGCTGTCCCAGGCGTTCAcctc     >  1:278068/1‑101 (MQ=255)                                cGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCg    >  1:3306738/1‑101 (MQ=255)                                 ggCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGa   >  2:2509242/1‑101 (MQ=255)                                 ggCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGa   >  2:1387819/1‑101 (MQ=255)                                  gCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAc  <  1:3958314/101‑1 (MQ=255)                                  gCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAc  >  1:1068352/1‑101 (MQ=255)                                                     |                                                                                    GGGCCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTG.CCGGT.CG.A.CGTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAC  >  NC_014034/774597‑774728 Base quality scores:  ATCG  < 3 ≤  ATCG  < 25 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG