Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 833,231 C→A 100% G35V (GGC→GTC)  mcpA1 ← methyl‑accepting chemotaxis protein McpA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034833,2310C→A100.0% 941.6 244G35V (GGC→GTC) mcpA1methyl‑accepting chemotaxis protein McpA
Reads supporting (aligned to +/- strand):  new base (139/105):  ref base (0/0):  total (139/105)

Only 100 of 250 total aligned reads displayed.

TGCCCGCTTGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGCCCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAATTTGTT  >  NC_014034/833133‑833328
                                                                                                  |                                                                                                 
tGCCCGCTTGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGacc                                                                                                 <  1:994420/101‑1 (MQ=255)
 gCCCGCTTGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCg                                                                                                <  2:678138/101‑1 (MQ=255)
    cGCTTGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCaa                                                                                             >  2:2184586/1‑101 (MQ=255)
      cTTGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCaaaa                                                                                           >  2:1635242/1‑101 (MQ=255)
      cTTGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCaaaa                                                                                           >  2:3777276/1‑101 (MQ=255)
       ttGCACCTGTGGTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAAc                                                                                          <  2:2235265/101‑1 (MQ=255)
       ttGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAAc                                                                                          >  1:3072412/1‑101 (MQ=255)
        tGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACg                                                                                         <  1:3606708/101‑1 (MQ=255)
        tGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACg                                                                                         >  2:922890/1‑101 (MQ=255)
           aCCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCAc                                                                                      >  1:2239014/1‑101 (MQ=255)
              tgtgCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAg                                                                                   <  1:4392940/101‑1 (MQ=255)
               gtgCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGc                                                                                  <  2:3821555/101‑1 (MQ=255)
                     ccAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCg                                                                            >  2:3913623/1‑101 (MQ=255)
                      cAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCgg                                                                           <  2:266178/101‑1 (MQ=255)
                        gatcgatcAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGAc                                                                         <  2:465161/101‑1 (MQ=255)
                           cgatcAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAg                                                                      <  2:1396504/101‑1 (MQ=255)
                           cgatcAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAg                                                                      <  2:585010/101‑1 (MQ=255)
                            gatcAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAga                                                                     >  1:3292256/1‑101 (MQ=255)
                             atcAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAgat                                                                    <  2:4013508/101‑1 (MQ=255)
                              tcAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGCGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGCCAAgatg                                                                   >  2:435290/1‑101 (MQ=255)
                                 gCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGatgatg                                                                <  2:3040702/101‑1 (MQ=255)
                                  cATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGAtgatga                                                               <  2:1922530/101‑1 (MQ=255)
                                  cATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGAtgatga                                                               <  1:1015870/101‑1 (MQ=255)
                                   aTGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAg                                                              >  1:4289912/1‑101 (MQ=255)
                                   aTGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAg                                                              <  2:4504484/101‑1 (MQ=255)
                                   aTGCCGATGGCGGTGTCGGTGACGTCGCTGACGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAg                                                              >  1:4186881/1‑101 (MQ=255)
                                      ccGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGa                                                           <  1:42534/101‑1 (MQ=255)
                                      ccGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGa                                                           <  2:3653392/101‑1 (MQ=255)
                                       cGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGaa                                                          <  2:4435799/101‑1 (MQ=255)
                                         aTGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAAc                                                        <  1:2072122/101‑1 (MQ=255)
                                          tGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAAcg                                                       >  2:776793/1‑101 (MQ=255)
                                           ggCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAAcgg                                                      >  1:4244213/1‑101 (MQ=255)
                                              ggTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAAcggcgg                                                   >  1:1815956/1‑101 (MQ=255)
                                               gtgtCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACggcggc                                                  <  1:1058579/101‑1 (MQ=255)
                                                 gtCGGTGACGTCGCTGAGGTGTTGTTAGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCaa                                                >  1:545240/1‑101 (MQ=255)
                                                   cGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAgg                                              <  1:1471602/101‑1 (MQ=255)
                                                   cGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAgg                                              >  2:2750264/1‑101 (MQ=255)
                                                   cGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAgg                                              >  1:3804503/1‑101 (MQ=255)
                                                     gTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGc                                            >  1:287131/1‑101 (MQ=255)
                                                      tGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGAGGCAAGGGcc                                           >  1:4529102/1‑101 (MQ=255)
                                                       gACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCa                                          <  1:3420530/101‑1 (MQ=255)
                                                          gTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCGACGa                                       >  2:4667074/1‑101 (MQ=255)
                                                          gTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGa                                       >  2:2343070/1‑101 (MQ=255)
                                                           tCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGAt                                      <  2:3813184/101‑1 (MQ=255)
                                                            cGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATc                                     >  1:1420800/1‑101 (MQ=255)
                                                            cGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATc                                     >  1:2963476/1‑101 (MQ=255)
                                                             gCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCg                                    >  1:2456073/1‑101 (MQ=255)
                                                              cTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGc                                   >  1:2743016/1‑101 (MQ=255)
                                                               tGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCa                                  <  1:2882118/101‑1 (MQ=255)
                                                               tGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCa                                  >  1:400412/1‑101 (MQ=255)
                                                               tGAGGGGTTGGTCGTGCATCTCATAGGCTTTCGAAACCGCAAAACGCACGAGCGTTTCGGACAAGATGATGAGCGAGACGGCGGCAAGGGCTAAGATccta                                  >  2:2215731/1‑98 (MQ=255)
                                                                 aGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATa                                >  2:2987467/1‑101 (MQ=255)
                                                                  ggTGTTGTTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGGGCGAAACGGCGGCAAGGGCCACGATAGCATAg                               >  1:763484/1‑101 (MQ=255)
                                                                    tgttgtTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGAt                             <  2:2892355/101‑1 (MQ=255)
                                                                    tgttgtTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGAt                             <  1:2561683/101‑1 (MQ=255)
                                                                    tgttgtTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGAt                             <  1:1594792/101‑1 (MQ=255)
                                                                    tgttgtTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGAt                             <  1:355581/101‑1 (MQ=255)
                                                                    tgttgtTCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGAt                             <  1:1218346/101‑1 (MQ=255)
                                                                      ttgttCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATgc                           >  1:4657809/1‑101 (MQ=255)
                                                                         ttCGCGCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATgcggg                        <  1:687505/101‑1 (MQ=255)
                                                                           cgcgCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATgcggggg                      >  1:1697121/1‑99 (MQ=255)
                                                                           cgcgCATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATgcgggcg                      >  2:3572840/1‑101 (MQ=255)
                                                                            gcgcATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATgcgggcgg                     <  2:545192/101‑1 (MQ=255)
                                                                             cgcATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGa                    <  2:520788/101‑1 (MQ=255)
                                                                             cgcATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGa                    >  2:2040133/1‑101 (MQ=255)
                                                                              gcATGTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGaa                   <  1:3662649/101‑1 (MQ=255)
                                                                              gcATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGaa                   >  1:1765543/1‑101 (MQ=255)
                                                                              gcATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGaa                   >  1:914839/1‑101 (MQ=255)
                                                                              gcATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGaa                   <  1:4161559/101‑1 (MQ=255)
                                                                              gcATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGAGGCGGGCGGaa                   >  2:2310966/1‑101 (MQ=255)
                                                                               cATCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGaaa                  <  2:421632/101‑1 (MQ=255)
                                                                                aTCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGaaaa                 >  2:1338245/1‑100 (MQ=255)
                                                                                aTCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAAt                 <  1:2810724/101‑1 (MQ=255)
                                                                                aTCTCATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAAt                 >  2:1120078/1‑101 (MQ=255)
                                                                                 tctcATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATa                >  1:4069298/1‑101 (MQ=255)
                                                                                   tcATCGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATAcc              >  1:2102552/1‑101 (MQ=255)
                                                                                   tcATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATAcc              >  2:1169198/1‑101 (MQ=255)
                                                                                   tcATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATAcc              >  2:183952/1‑101 (MQ=255)
                                                                                    cATAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCg             >  2:4323548/1‑101 (MQ=255)
                                                                                      tAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCgaa           >  1:2154091/1‑101 (MQ=255)
                                                                                      tAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCgaa           >  1:520848/1‑101 (MQ=255)
                                                                                      tAGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCgaa           >  2:280967/1‑101 (MQ=255)
                                                                                       aGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCgaag          <  1:3913908/101‑1 (MQ=255)
                                                                                       aGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCgaag          <  1:3781618/101‑1 (MQ=255)
                                                                                       aGGCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCgaag          <  2:3323599/101‑1 (MQ=255)
                                                                                        ggCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCgaaga         >  2:3691699/1‑101 (MQ=255)
                                                                                        ggCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCgaaga         <  1:1992850/101‑1 (MQ=255)
                                                                                        ggCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCgaaga         <  2:4185561/101‑1 (MQ=255)
                                                                                        ggCGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCgaaga         <  2:3339976/101‑1 (MQ=255)
                                                                                          cGTTCGCGACCGCAAAACGCACCAGCGTTTCGGACAAGATGAAGAGCGAAACGGCGGCAAAGGCCACAACCGCATAGATGCGGGGGGAAATTGCGGAGAAt       >  2:1721421/1‑101 (MQ=255)
                                                                                          cGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAAt       <  1:3476481/101‑1 (MQ=255)
                                                                                          cGTTCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAAt       <  2:2445044/101‑1 (MQ=255)
                                                                                            ttCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAAttt     <  1:1617821/101‑1 (MQ=255)
                                                                                            ttCGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAAttt     <  1:446792/101‑1 (MQ=255)
                                                                                              cGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAATTTGt   <  2:2534341/101‑1 (MQ=255)
                                                                                              cGAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAATTTGt   >  1:1860942/1‑101 (MQ=255)
                                                                                               gAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGCATTTGtt  >  2:1800832/1‑101 (MQ=255)
                                                                                               gAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAATTTGtt  <  1:1159060/101‑1 (MQ=255)
                                                                                               gAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAATTTGtt  <  1:3697675/101‑1 (MQ=255)
                                                                                               gAGACCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAATTTGtt  >  2:2138504/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
TGCCCGCTTGCACCTGTGCTTCCAGATCGATCAGCATGCCGATGGCGGTGTCGGTGACGTCGCTGAGGTGTTGTTCGCGCATCTCATAGGCGTTCGAGCCCGCAAAACGCACCAGCGTTTCGGACAAGATGATGAGCGAAACGGCGGCAAGGGCCACGATCGCATAGATGCGGGCGGAAATACCGAAGAATTTGTT  >  NC_014034/833133‑833328

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG