Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	974,361	C→G	100%	intergenic (+241/+18)	RCAP_rcc00884 → / ← RCAP_rcc00885	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	974,361	0	C→G	100.0%	254.2	66	intergenic (+241/+18)	RCAP_rcc00884/RCAP_rcc00885	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (38/28):  ref base (0/0):  total (38/28)

TTTTCACCTTGCGACAAATATCCC.GGGGT..GTGCG.GAAGCCCCCGGCGGGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCCGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAGCGCCC  >  NC_014034/974280‑974458                                                                                      |                                                                                                  ttttCACCTTGCGACAAATATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGGGGCTCCGCGGCAGCCCCCGCGGAAAAACCCCGCCGCGCCGGcccgg                                                                                    >  2:3806309/1‑98 (MQ=0)     cACCTTGCGACAAATATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGCGCCTCGGCGGCAGCCGAGGCGAGAAAACCCGGCCGCCACGGCCGGGtctc                                                                                >  1:1790088/1‑101 (MQ=9)                                     ggggcccccccGGCGGGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATc                                                <  1:1336891/95‑1 (MQ=255)                                         ccgcccgcgcggGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCAccc                                            <  1:1871718/93‑1 (MQ=255)                                          gCCCCCGGCGGGGCGCCCCGGCGACAGCCGAGGCGAAAAAACCCCGCCGCAACGGCCCGGTCTCAGCCGAACAAGAACACGGTGGAGGGCGCGAGCACCCa                                           >  2:1872503/1‑101 (MQ=255)                                          cccccgGGCGGGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCa                                           <  2:65179/100‑1 (MQ=255)                                           cccccGGCGGGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCag                                          <  2:573860/101‑1 (MQ=255)                                             ccgggcggGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCagag                                        <  1:2736060/98‑1 (MQ=255)                                              ggggcggGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAgg                                       <  1:3070152/99‑1 (MQ=255)                                              cgggcggGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAgg                                       <  1:1217660/99‑1 (MQ=255)                                                 gcggGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCCACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATGACCCAGAGGGtc                                    >  2:1438967/1‑101 (MQ=255)                                                 gcggGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAggggc                                    >  1:1840340/1‑99 (MQ=255)                                                  cggGTCGCCTCGGCGACAGCCGAGGCGAAATCCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtca                                   <  2:1592121/101‑1 (MQ=255)                                                  cggGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtca                                   >  1:1322961/1‑101 (MQ=255)                                                    ggTCGCCTCGTCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtcatc                                 <  2:281332/101‑1 (MQ=255)                                                    ggTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtcatc                                 <  1:3536618/101‑1 (MQ=255)                                                    ggTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGccatc                                 >  2:1806911/1‑101 (MQ=255)                                                     gTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtcatca                                >  1:3830103/1‑101 (MQ=255)                                                     gTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtcatca                                <  2:1322868/101‑1 (MQ=255)                                                     gTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGgcatca                                >  2:4405219/1‑101 (MQ=255)                                                      tCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAg                               >  2:3404872/1‑101 (MQ=255)                                                       cGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAgc                              <  2:3133/101‑1 (MQ=255)                                                       cGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAgc                              <  2:1623557/101‑1 (MQ=255)                                                       cGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCCATCACCCAGAGGGTCATCAgc                              <  1:2647708/101‑1 (MQ=255)                                                        gCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAgcg                             >  1:1322691/1‑101 (MQ=255)                                                        gCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAgcg                             >  2:2378690/1‑101 (MQ=255)                                                         ccTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAgcgc                            <  2:591157/101‑1 (MQ=255)                                                          cTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCa                           >  1:971652/1‑101 (MQ=255)                                                           tCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCAt                          >  2:1000858/1‑101 (MQ=255)                                                           tCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCAt                          >  2:1661925/1‑101 (MQ=255)                                                            cGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATa                         >  1:179910/1‑101 (MQ=255)                                                            cGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATa                         >  1:3625068/1‑101 (MQ=255)                                                             ggCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAg                        <  1:3778053/101‑1 (MQ=255)                                                              gCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAgg                       <  1:1350468/101‑1 (MQ=255)                                                              gCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAgg                       <  2:3383513/101‑1 (MQ=255)                                                              gCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAgg                       >  1:2580444/1‑101 (MQ=255)                                                               cGACAGCCGAGGCGAAATCCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGc                      <  1:319792/101‑1 (MQ=255)                                                                gACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGcc                     >  2:1550052/1‑101 (MQ=255)                                                                gACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGcc                     >  2:2563474/1‑101 (MQ=255)                                                                gACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGcc                     >  1:1021633/1‑101 (MQ=255)                                                                  cAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGAGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGc                   >  1:3937806/1‑101 (MQ=255)                                                                    gCCGAGGCGAAATCCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCaa                 <  1:3365658/101‑1 (MQ=255)                                                                     ccGAGGCGAAATCCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCaaa                <  2:736195/101‑1 (MQ=255)                                                                     ccGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCaaa                >  1:2237901/1‑101 (MQ=255)                                                                     ccGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCaaa                <  2:2043880/101‑1 (MQ=255)                                                                      cGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAc               >  2:3332994/1‑101 (MQ=255)                                                                       gAGGCGAAATGCCCGGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAAGTTCGGGCGCGCGATCGCCCAGAGGGTCATCAGCGCATAGGCCGCAAAcc              <  1:4637382/101‑1 (MQ=255)                                                                       gAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAcc              >  1:3892603/1‑101 (MQ=255)                                                                       gAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAcc              >  1:3136568/1‑101 (MQ=255)                                                                       gAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAcc              >  1:2759929/1‑101 (MQ=255)                                                                       gAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAcc              >  2:3970015/1‑101 (MQ=255)                                                                       gAGGCGAAATACCCCGGCCGCAACGGCCGGTTCCCAGCCGAACAGGACTAGGTTCGCGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGACGGCAAcc              >  1:847493/1‑101 (MQ=255)                                                                        aGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAccc             >  2:565702/1‑101 (MQ=255)                                                                        aGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAccc             <  1:4588904/101‑1 (MQ=255)                                                                         ggCGAAATCCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAcccc            <  2:1019797/101‑1 (MQ=255)                                                                         ggCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAcccc            <  1:2348152/101‑1 (MQ=255)                                                                         ggCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAcccc            >  1:2744095/1‑101 (MQ=255)                                                                         ggCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAcccc            <  2:2346540/101‑1 (MQ=255)                                                                          gCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCg           <  2:2915212/101‑1 (MQ=255)                                                                          gCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCg           >  1:3155266/1‑101 (MQ=255)                                                                            gAAATCCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGcc         <  1:4109776/101‑1 (MQ=255)                                                                            gAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGcc         >  2:600504/1‑101 (MQ=255)                                                                            gAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGcc         >  1:1749739/1‑101 (MQ=255)                                                                            gAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGcc         >  1:2406487/1‑101 (MQ=255)                                                                             aaaTACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCa        >  2:789599/1‑101 (MQ=255)                                                                              aaTACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAg       >  1:1921250/1‑101 (MQ=255)                                                                                tACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAgcg     >  2:3383632/1‑101 (MQ=255)                                                                                 aCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAgcgc    >  2:4011133/1‑101 (MQ=255)                                                                                 aCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAgcgc    >  1:3265674/1‑101 (MQ=255)                                                                                 aCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAgcgc    >  2:3089505/1‑101 (MQ=255)                                                                                 aCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAgcgc    >  1:1062186/1‑101 (MQ=255)                                                                                  ccccgGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAGCGcc   >  1:4419462/1‑101 (MQ=255)                                                                                  ccccgGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAGAGcc   <  2:3892318/101‑1 (MQ=255)                                                                                   cccgGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCCTCAGCGCATAGGCCGCAAACCCCGCCAGCGccc  >  1:4020279/1‑101 (MQ=255)                                                                                   cccgGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAGCGccc  <  1:141551/101‑1 (MQ=255)                                                                                   cccgGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAGCGccc  >  1:1362097/1‑101 (MQ=255)                                                                                      |                                                                                                  TTTTCACCTTGCGACAAATATCCC.GGGGT..GTGCG.GAAGCCCCCGGCGGGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCCGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAGCGCCC  >  NC_014034/974280‑974458 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG