Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,732,868	2 bp→GT	100%	intergenic (‑292/‑110)	RCAP_rcc01601 ← / → tgt	FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,732,868	0	T→G	100.0%	64.2	30	intergenic (‑292/‑111)	RCAP_rcc01601/tgt	FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (7/22):  ref base (0/0):  total (7/23)
*	NC_014034	1,732,869	0	C→T	100.0%	65.8	30	intergenic (‑293/‑110)	RCAP_rcc01601/tgt	FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (7/22):  ref base (0/1):  total (7/23)

AACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATA.CGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG..CCCCCC....GT...C....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCT.TTCGGCCCGGC  >  NC_014034/1732790‑1732948                                                                                      |||||                                                                                      aaCAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATA.CGCCCTTCTTTCTTTGCGGGGGGGCGGGGGGTGAAG..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGa                                                                 <  1:3070274/101‑1 (MQ=21)      gCAAGGGCCCCGCTTTTCCTAGGCAAAATA.CGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.Ga                                                           <  1:3828337/101‑1 (MQ=21)      gCAAGGCCCCTGTTTTTCCCAGGGAAAATT.CCCCTTTTTTTTTTTGGGGGGGGGGGGGGGGGGCCC..CCCCCC....GG...CGGGTCGCCGCGGGCTTGCCCGAGGC.g                                                            <  2:2473163/101‑1 (MQ=2)        aaGGGCCCCGCTTTTCCTAGGCAAAATA.CGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG..CCCCCC....GCGGGT....CGCCTCGGGCTTGCCCGAGGC.Gaaa                                                         <  2:625536/101‑1 (MQ=18)         agacccctGCTTTTCCTAGGAAAATGC.CGCCCTTTTTTC.TTTCGGGGGGGGCGGGGGTAAAG..CCCCCCCGCGGG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAg                                                        <  2:2041340/97‑1 (MQ=2)              cccGTTTTTCCCAGGCAAAATG.CGCCCTTCTTTCTTTTGGGGGGGGGGGGGGGTGAAG..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTcc                                                   <  1:2131675/101‑1 (MQ=17)              cccGCTTTTCCTAGGCAAAATC.CGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTcc                                                   <  1:2819242/101‑1 (MQ=18)                 gCTTTTCCTAGGCAAAATA.CGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAC..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTccccc                                                <  1:612871/101‑1 (MQ=21)                  cTTTTCCTAGGCAAAATC.CGCCCTGCTTTCTTTGCGGGGGGGCGGGGGGTGAAG..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTcccccc                                               <  2:2216636/101‑1 (MQ=17)                       ccTAGGCAAAATA.CGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTCCCCCCGTGaa                                          <  2:3038981/101‑1 (MQ=21)                         tAGGCAAAATA.CGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTCCCCCCGTGaaaa                                        <  1:4631361/101‑1 (MQ=21)                         tAGCCAAAATC.CGCCTTTTTTTCTTTGGGGGGGGGCGGGGGGTAAAC..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTCCCCCCGTGaaaa                                        <  1:1388593/101‑1 (MQ=9)                         caagCAAAATCCCGCCTTTTTTTCTTTGGGGGGGGGGGGGGGGTAACC..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTCCCCCCGTGaaa                                         <  2:4139376/98‑1 (MQ=2)                           ggCAAAATA.CGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTCCCCCCGTGAAAAcc                                      <  1:2228877/101‑1 (MQ=21)                            gaaaagTA.CCCCCTTCTTTCTTTCGGGGGGGGCGGGGGGTGAAC..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTCCCCCCGTGAAAACCt                                     <  2:2568441/99‑1 (MQ=17)                                aaTA.CGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGGGAAGGCCCCCCCG...GG...G....GGCCTCGGGGTTGGCCGCGGCGGGAGGCTCCCCCCCCTGAAAActcgcc                                  >  2:3650040/1‑96 (MQ=255)                                 aTA.CGCCCTTCTTTCTTTTGGGGGGGGCGGGGGGTGAAG..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTCCCCCCGTGAAAACCTTGCCt                                <  2:4638946/101‑1 (MQ=18)                                  atccGCCTTGCTTTCTTTGGGGGGGGGGGGGGGGTGAAG..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTCCCCCCGTGAAAACCTTGCCt                                <  2:4515289/98‑1 (MQ=9)                                   tccGCCTTTTTTTCTTTTGGGGGGGGGGGGGGGTGAGC..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTCCCCCCGTGAAAACCTTGCCTg                               <  1:1265839/99‑1 (MQ=12)                                        ccTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTCCCCCCGTGAAAACCTTGCCTgcgcgc                          <  2:2866880/101‑1 (MQ=21)                                          ttctttttTTTGGGGGGGGGGGGGGGTAAAC..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGt                        <  1:2787169/101‑1 (MQ=18)                                                    tggggggggGGGGGGGGGAAC..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCt              <  1:1178798/99‑1 (MQ=18)                                                    gggggggggGGGGGGGTGACG..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCt              <  1:4502018/99‑1 (MQ=18)                                                     ggggggggGGGGGGGTGAAG..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCt.t            <  1:2841421/100‑1 (MQ=18)                                                      gggggggGGGGGGGGGACG..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCt.tt           <  1:331732/101‑1 (MQ=18)                                                              ggggggTGAAG..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTCCCCCCGTGAAAACCTTGGCTGCGCGCGTCGGTTTCCCT.TTCGCCCCgg   >  2:1558645/1‑101 (MQ=18)                                                              ggggggTGAAG..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCT.TTCCGccccg   >  1:4429828/1‑99 (MQ=21)                                                              ggggggTGAAG..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTCCCCCCCTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCT.TTCGGCCCgg   >  1:1171363/1‑101 (MQ=21)                                                              ggggggTGAAG..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTCCCCCCCTGAAAACCTTGCCTGCGCGCGGCGCTTTCCCT.TTCGGccccg   >  2:3513571/1‑99 (MQ=18)                                                              ggggggTGAAG..CCCCCCGCG.GG...G....CGCCCCGGGCTTGCCCCCGGG.GGAAACTTCCCCCCGCGAAAAACCTGCCCGCGCGCGCCGCCTTCCCTCTTCGCCCCg    >  1:3965202/1‑101 (MQ=255)                                                               gggggTGAAG..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCT.TTCGGCCCggg  >  2:1492929/1‑100 (MQ=21)                                                               gggggTGAAG..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCT.TTCGGCCCGgc  >  2:2282214/1‑101 (MQ=21)                                                               gggggTGAAG..CCCCCCGCG.GG...T....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCT.TTCGGCCCGgc  >  2:1473257/1‑101 (MQ=21)                                                                                      |||||                                                                                      AACAGGCAAGGGCCCCGCTTTTCCTAGGCAAAATA.CGCCCTTCTTTCTTTTCGGGGGGGCGGGGGGTGAAG..CCCCCC....GT...C....CGCCTCGGGCTTGCCCGAGGC.GAAAGCTTCCCCCCGTGAAAACCTTGCCTGCGCGCGTCGCTTTCCCT.TTCGGCCCGGC  >  NC_014034/1732790‑1732948 Base quality scores:  ATCG  < 3 ≤  ATCG  < 7 ≤  ATCG  < 18 ≤  ATCG  < 30 ≤  ATCG  < 39 ≤  ATCG