Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,733,287 2 bp→GC 100% coding (309‑310/1128 nt) tgt → queuine tRNA‑ribosyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,733,2870C→G100.0% 689.1 184S103S (TCC→TCGtgtqueuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (90/94):  ref base (0/0):  total (90/94)
*NC_0140341,733,2880G→C100.0% 685.5 183M104M (GTG→CTG) tgtqueuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (89/94):  ref base (0/0):  total (89/94)

Only 100 of 196 total aligned reads displayed.

CGCATCGCGCGGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCCGTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCAGAA  >  NC_014034/1733192‑1733382
                                                                                               ||                                                                                              
 gcATCGCGCGGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTgcgc                                                                                           <  2:3919408/101‑1 (MQ=255)
  cATCGCGCGGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCa                                                                                          <  1:4286145/101‑1 (MQ=255)
   aTCGCGCGGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCaa                                                                                         >  1:2320859/1‑101 (MQ=255)
   aTCGCGCGGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCaa                                                                                         <  2:4390419/101‑1 (MQ=255)
   aTCGCGCGGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCaa                                                                                         <  1:2368382/101‑1 (MQ=255)
    tCGCGCGGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAg                                                                                        <  1:1459272/101‑1 (MQ=255)
    tCGCGCGGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAg                                                                                        <  2:2532477/101‑1 (MQ=255)
      gcgcgGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCt                                                                                      <  2:641346/101‑1 (MQ=255)
        gcgGCTGGGCGGGTTGCACAAGTTCATGAACTGGGGGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGa                                                                                    <  1:2349987/101‑1 (MQ=255)
         cgGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGAc                                                                                   <  2:1664903/101‑1 (MQ=255)
          ggCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGAcc                                                                                  <  1:583910/101‑1 (MQ=255)
          ggCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGAcc                                                                                  <  1:2549903/101‑1 (MQ=255)
          ggCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGAcc                                                                                  <  1:1434271/101‑1 (MQ=255)
          ggCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGAcc                                                                                  >  1:4172764/1‑101 (MQ=255)
           gCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCg                                                                                 <  2:3628347/101‑1 (MQ=255)
           gCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCg                                                                                 <  1:3240670/101‑1 (MQ=255)
            cTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGGGATGAGCCTTGCTTCGCTGCGCAAGCTGACCga                                                                                >  1:3977139/1‑101 (MQ=255)
             tGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCgag                                                                               <  1:2085481/101‑1 (MQ=255)
             tGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCgag                                                                               <  1:1206410/101‑1 (MQ=255)
              gggCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCgagg                                                                              <  1:2700462/101‑1 (MQ=255)
              gggCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCgagg                                                                              >  1:2917008/1‑101 (MQ=255)
               ggCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCgagga                                                                             >  1:1896379/1‑101 (MQ=255)
                gCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCgaggag                                                                            >  1:4605838/1‑101 (MQ=255)
                gCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCgaggag                                                                            <  1:3279498/101‑1 (MQ=255)
                 cGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGaggagg                                                                           >  2:4578278/1‑101 (MQ=255)
                 cGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGaggagg                                                                           <  1:3591735/101‑1 (MQ=255)
                 cGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGaggagg                                                                           <  2:3435469/101‑1 (MQ=255)
                 cGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGaggagg                                                                           <  2:3346112/101‑1 (MQ=255)
                 cGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGaggagg                                                                           >  2:2230877/1‑101 (MQ=255)
                  gggTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAggg                                                                          >  1:133340/1‑101 (MQ=255)
                   ggTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGc                                                                         >  1:3114914/1‑101 (MQ=255)
                   ggTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGc                                                                         <  2:133326/101‑1 (MQ=255)
                   ggTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGc                                                                         <  1:434945/101‑1 (MQ=255)
                    gTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCg                                                                        <  1:3050365/101‑1 (MQ=255)
                    gTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCg                                                                        >  2:1267873/1‑101 (MQ=255)
                     ttGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGt                                                                       <  1:2801905/101‑1 (MQ=255)
                      tGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGGCTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTg                                                                      <  1:2585757/101‑1 (MQ=255)
                      tGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTg                                                                      >  2:954309/1‑101 (MQ=255)
                      tGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTg                                                                      <  1:1099826/101‑1 (MQ=255)
                        cacaAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGAc                                                                    <  2:406668/101‑1 (MQ=255)
                        cacaAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGAc                                                                    >  2:2333332/1‑101 (MQ=255)
                          caAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCt                                                                  <  2:1026790/101‑1 (MQ=255)
                          caAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCt                                                                  <  1:1019962/101‑1 (MQ=255)
                           aaGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCtt                                                                 <  2:1937984/101‑1 (MQ=255)
                            aGTTCCTGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCttt                                                                <  1:379372/101‑1 (MQ=255)
                              ttCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTc                                                              <  1:500953/101‑1 (MQ=255)
                              ttCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTc                                                              <  1:4595987/101‑1 (MQ=255)
                               tCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTcc                                                             <  2:3453417/101‑1 (MQ=255)
                               tCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTcc                                                             >  2:1245961/1‑101 (MQ=255)
                                 aTGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAg                                                           <  2:3265372/101‑1 (MQ=255)
                                 aTGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAg                                                           >  2:4233434/1‑101 (MQ=255)
                                 aTGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAg                                                           >  2:85119/1‑101 (MQ=255)
                                   gAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGcc                                                         <  2:553801/101‑1 (MQ=255)
                                    aaCTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCa                                                        >  2:1505540/1‑101 (MQ=255)
                                    aaCTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCa                                                        <  2:4178303/101‑1 (MQ=255)
                                     aCTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCat                                                       <  1:4572497/101‑1 (MQ=255)
                                            gcgcCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACg                                                <  2:684577/101‑1 (MQ=255)
                                            gcgcCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACg                                                >  2:2774068/1‑101 (MQ=255)
                                              gcCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGc                                              >  2:497927/1‑101 (MQ=255)
                                              gcCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGc                                              <  1:3741279/101‑1 (MQ=255)
                                               ccgaacccggACCGACTCGGGCGGGTTTCAGGTGATGACCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCt                                             <  1:1282619/92‑1 (MQ=255)
                                                ccGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTc                                            <  2:607418/101‑1 (MQ=255)
                                                 ggATCCTGACCGGCTGGGGGGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCGGACCGAGGAGGGCCTGACCTTTTCCAGCCATATCGACGGCTCg                                           <  2:3823777/100‑1 (MQ=255)
                                                  gATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGa                                          >  1:2216142/1‑101 (MQ=255)
                                                      cTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAaca                                      >  2:4622895/1‑101 (MQ=255)
                                                       tGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAacac                                     >  2:3237923/1‑101 (MQ=255)
                                                       tGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAacac                                     >  1:1638385/1‑101 (MQ=255)
                                                       tGACCGACTCGGGCGGGTTTCAGGTGATGAGACTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAacac                                     >  1:4142707/1‑101 (MQ=255)
                                                          ccGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCAt                                  >  2:3411314/1‑101 (MQ=255)
                                                            gACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACGGAGGAGGGCGTGTCCTTTTCCAGCCATATCGACGGCTCGAAACACCATCt                                <  1:3948777/101‑1 (MQ=255)
                                                              cTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGt                              <  1:3569274/101‑1 (MQ=255)
                                                              cTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGt                              >  2:2852834/1‑101 (MQ=255)
                                                               tCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTc                             <  2:3264354/101‑1 (MQ=255)
                                                               tCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTc                             >  1:2821041/1‑101 (MQ=255)
                                                               tCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTc                             >  1:1010303/1‑101 (MQ=255)
                                                                 gggcgggTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGc                           >  1:1587805/1‑101 (MQ=255)
                                                                 gggcgggTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGc                           >  2:2061039/1‑101 (MQ=255)
                                                                 gggcgggTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGc                           >  1:3406937/1‑101 (MQ=255)
                                                                  ggcgggTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGcc                          <  2:1875485/101‑1 (MQ=255)
                                                                     gggTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGa                       >  1:1243996/1‑101 (MQ=255)
                                                                      ggTTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGaa                      >  1:1852257/1‑101 (MQ=255)
                                                                        tttCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGaagc                    >  2:1157202/1‑99 (MQ=255)
                                                                         ttCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGc                   >  2:2872791/1‑101 (MQ=255)
                                                                          tCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCNTCTGTCgnnnnnnnnnn                  >  2:1497595/1‑91 (MQ=255)
                                                                           cAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTc                 >  1:1768886/1‑101 (MQ=255)
                                                                           cAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTc                 <  1:4120386/101‑1 (MQ=255)
                                                                              gTGATGAGCCTTGCTTCGCTGTGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGAt              >  1:3437907/1‑101 (MQ=255)
                                                                              gTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGAt              >  2:3015663/1‑101 (MQ=255)
                                                                               tgatgaGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATg             <  1:639404/101‑1 (MQ=255)
                                                                               tgatgaGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATg             >  1:2612613/1‑101 (MQ=255)
                                                                               tgatgaGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATg             <  1:674969/101‑1 (MQ=255)
                                                                                gatgaCCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGCCCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATgg            <  1:2774266/101‑1 (MQ=255)
                                                                                 atgaGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGga           >  2:1635804/1‑101 (MQ=255)
                                                                                 atgaGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGga           <  2:3724492/101‑1 (MQ=255)
                                                                                   gaGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGgaga         >  1:592549/1‑101 (MQ=255)
                                                                                       cTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCGATATCGACGGCTCGAAAGACCCTCTGTCGTCCGAACGCGCGATGGAGATccg     >  2:4500270/1‑100 (MQ=255)
                                                                                       cTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACCCTCGATGCAGATccc     >  2:1000000/1‑100 (MQ=255)
                                                                                        ttGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCCCGATGGAGATCCAg    >  1:2220949/1‑101 (MQ=255)
                                                                                         tGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCata   >  2:4648380/1‑99 (MQ=255)
                                                                                         tGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCAGa   >  1:3856100/1‑101 (MQ=255)
                                                                                               ||                                                                                              
CGCATCGCGCGGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGGTTTCAGGTGATGAGCCTTGCTTCCGTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCAGAA  >  NC_014034/1733192‑1733382

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG