Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,740,784 2 bp→GC 100% intergenic (+21/+113) cxp → / ← RCAP_rcc01610 thermostable carboxypeptidase 1/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,740,7840C→G100.0% 588.1 172intergenic (+21/+114)cxp/RCAP_rcc01610thermostable carboxypeptidase 1/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (75/96):  ref base (0/1):  total (75/97)
*NC_0140341,740,7850G→C100.0% 598.1 175intergenic (+22/+113)cxp/RCAP_rcc01610thermostable carboxypeptidase 1/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (75/96):  ref base (0/4):  total (75/100)

Only 100 of 187 total aligned reads displayed.

GCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GA.GGG.GCGCTGCCCCCCG.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGG  >  NC_014034/1740690‑1740880
                                                                                                   ||                                                                                                
gCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAg                                                                                             <  2:4361166/101‑1 (MQ=255)
gCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAg                                                                                             >  2:203390/1‑101 (MQ=255)
 ggggGTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAgg                                                                                            <  1:1141263/100‑1 (MQ=255)
 cGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCACGC.CGAgg                                                                                            >  1:2017470/1‑101 (MQ=255)
  gggTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGc                                                                                           >  2:4217038/1‑101 (MQ=255)
   ggTTGGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCg                                                                                          <  1:106010/101‑1 (MQ=255)
     ttCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGAt                                                                                        <  1:2268301/101‑1 (MQ=255)
       cGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGc                                                                                      >  1:3195371/1‑101 (MQ=255)
        gAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGcc                                                                                     >  1:1484095/1‑101 (MQ=255)
           ccGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCg                                                                                  <  1:4316136/101‑1 (MQ=255)
             gACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGc                                                                                >  2:4271681/1‑101 (MQ=255)
              aCGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGcc                                                                               <  1:3106297/101‑1 (MQ=255)
               cGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGccc                                                                              >  2:3973148/1‑101 (MQ=255)
               cGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGccc                                                                              >  1:4451238/1‑101 (MQ=255)
                ggAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GGGCTGCCCCCGC.CGGGGCGAGGGCTCGCcccc                                                                             >  1:4350859/1‑101 (MQ=255)
                 gaagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCGCCCGC.CGAGGCGATGCCTCGGccccc                                                                            >  2:273703/1‑101 (MQ=255)
                 gaagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGccccc                                                                            <  2:4320121/101‑1 (MQ=255)
                  aagaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGcccccc                                                                           >  1:2902630/1‑101 (MQ=255)
                    gaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCgg                                                                         >  1:1549032/1‑101 (MQ=255)
                    gaaCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCgg                                                                         >  1:3650312/1‑101 (MQ=255)
                      aCCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGa                                                                       <  2:3596651/101‑1 (MQ=255)
                       ccGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGat                                                                      <  1:3324044/101‑1 (MQ=255)
                         gCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGatat                                                                    <  2:552010/101‑1 (MQ=38)
                          cTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTCCCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATAtt                                                                   <  2:3228911/101‑1 (MQ=38)
                          cTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCAGC.CGAGGCGATGCCTCGGCCCCCCGGGATAtt                                                                   >  2:4629692/1‑101 (MQ=38)
                            tCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                                 >  2:3685704/1‑101 (MQ=38)
                            tCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GA.GGGTGCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTc                                                                 >  1:3924174/1‑101 (MQ=38)
                             cTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCg                                                                >  1:3173153/1‑101 (MQ=38)
                             cTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCg                                                                >  2:214703/1‑101 (MQ=38)
                              tGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGa                                                               >  2:688882/1‑101 (MQ=38)
                              tGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGa                                                               <  2:4539583/101‑1 (MQ=38)
                               ggACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAg                                                              >  1:1684777/1‑101 (MQ=38)
                                gACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGc                                                             <  1:3035160/101‑1 (MQ=38)
                                 aCTATCTCGAAAGCAAATACGCCGAGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCa                                                            <  1:4672360/101‑1 (MQ=38)
                                  cTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCaa                                                           >  2:298661/1‑101 (MQ=38)
                                  cTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCGGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCaa                                                           <  2:2837022/101‑1 (MQ=38)
                                   tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                                          >  2:1410588/1‑101 (MQ=38)
                                   tATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAg                                                          >  2:1515761/1‑101 (MQ=38)
                                    aTCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGa                                                         >  1:84809/1‑101 (MQ=38)
                                      ctcGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATg                                                       <  2:4507447/101‑1 (MQ=37)
                                      ctcGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATg                                                       <  1:3217946/101‑1 (MQ=37)
                                         gAAAGCAAATACGCCGCGATCTCCCGGCTTT.GAGGG.C.GAGGGG.GCGCGGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                                    <  1:3588409/101‑1 (MQ=37)
                                         gAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                                    >  2:1743396/1‑101 (MQ=37)
                                         gAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGaaa                                                    >  2:1822063/1‑101 (MQ=37)
                                          aaaGCAAATCCGCCGCGATCTACCGGCTTG.GGGGG.CGGG.GGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAg                                                   <  1:3570613/101‑1 (MQ=25)
                                          aaaGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAg                                                   >  2:4018677/1‑101 (MQ=37)
                                           aaGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAgg                                                  >  2:2482832/1‑101 (MQ=37)
                                            aGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGc                                                 <  1:3096605/101‑1 (MQ=38)
                                             gaaaaTGCGCCGCGATTTGCGGGCTTT.GAGGG.G.GAGGGG.GCGCTCCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCa                                                <  2:4006619/99‑1 (MQ=21)
                                             gCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCa                                                <  1:1001338/101‑1 (MQ=38)
                                               aaaTACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCTCCCGC.CGAGGCGATGCTTCGCCCCCCCGGGACATTTCTAGCAAGATGCAAggaaca                                              >  2:158508/1‑97 (MQ=255)
                                               aaaTACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATc                                              >  1:1098638/1‑101 (MQ=37)
                                               aaaTACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATc                                              >  2:1180149/1‑101 (MQ=37)
                                               aaaTACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGACCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATc                                              >  2:2217939/1‑101 (MQ=37)
                                                 aTACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCg                                            <  2:3321774/101‑1 (MQ=38)
                                                 aTACGCCGCGATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCg                                            >  2:3373645/1‑101 (MQ=37)
                                                  tccgccgcGATCTACCGGCTTT.GGGGG.CGGG.GGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCgg                                           <  1:927729/99‑1 (MQ=37)
                                                      ccgcGATCTACCGGTTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGTCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTc                                       <  2:4035570/101‑1 (MQ=25)
                                                      ccgcGATCTACCGGCTTG.GAGGG.C.GAGGGG.GCCCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTc                                       <  2:1483986/101‑1 (MQ=25)
                                                        gcgATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAATGCATCCGGTTTCAt                                     >  1:4331149/1‑101 (MQ=37)
                                                         cgATCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGGTTCATc                                    >  2:3646701/1‑101 (MQ=37)
                                                          gttCTACCGGGTTT.GGGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTCCATCt                                   <  1:2066040/99‑1 (MQ=25)
                                                           aTCTTCCGGTTTT.GGGGGTG.GG.GGG.GCGCTCCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCtt                                  <  1:1756379/101‑1 (MQ=18)
                                                           aTCTCCCGGTTTTGGGGGG.G.GG.GGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCtt                                  <  1:1905912/101‑1 (MQ=21)
                                                           aTCTACGGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCtt                                  <  2:2982871/101‑1 (MQ=25)
                                                            tCTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTg                                 >  1:2467835/1‑101 (MQ=25)
                                                             ttACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGc                                <  2:1934640/100‑1 (MQ=25)
                                                             cTACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGc                                <  2:4301707/101‑1 (MQ=25)
                                                              tACCGGCTTT.GAGGG.CGGG.GGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCt                               <  1:4516429/101‑1 (MQ=25)
                                                              tACCGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCt                               >  2:818558/1‑101 (MQ=25)
                                                               aCCGGCTTT.GGGGG.G.GAGGGG.GCGCTCCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCtt                              <  1:3350482/101‑1 (MQ=21)
                                                                ccGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTc                             <  2:2649912/101‑1 (MQ=25)
                                                                ccGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTc                             <  1:3605024/101‑1 (MQ=25)
                                                                ccGGCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTc                             <  1:690329/101‑1 (MQ=25)
                                                                   gCTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCaaa                          >  2:2660396/1‑101 (MQ=25)
                                                                    cTTT.GGGGG.G.GAGGGG.GCGCTCCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAAt                         <  2:371845/101‑1 (MQ=21)
                                                                    cTTT.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAAt                         >  2:1844771/1‑101 (MQ=25)
                                                                     ttt.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATa                        >  2:2562170/1‑101 (MQ=25)
                                                                      ttggAGGG.C.GAGGGG.GCGCTTCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATa                        <  2:3841516/98‑1 (MQ=21)
                                                                      tt.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAc                       >  1:4586553/1‑101 (MQ=25)
                                                                       t.GAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAcc                      >  1:1238080/1‑101 (MQ=25)
                                                                       gggAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATAc                       <  1:3444165/99‑1 (MQ=21)
                                                                         gAGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCt                     <  1:2775575/101‑1 (MQ=25)
                                                                          aGGG.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTc                    >  1:58374/1‑101 (MQ=25)
                                                                           ggg.C.GAGGGG.GCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCg                   >  1:2984131/1‑101 (MQ=25)
                                                                               gcgagggg.gCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCggg                 <  2:442844/96‑1 (MQ=255)
                                                                                 gagggg.gCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCggggg               <  1:689060/98‑1 (MQ=255)
                                                                                  agggg.gCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTGGAGCAAGATGAAAGGAATCCGGTTTCATCTTGCTTCAAATACCTCgggggg              >  1:3945594/3‑101 (MQ=25)
                                                                                   gggg.gCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGa             >  2:668434/2‑101 (MQ=255)
                                                                                   gggg.gCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGa             >  2:806469/2‑101 (MQ=255)
                                                                                    ggg.gCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAg            <  1:1393534/101‑1 (MQ=255)
                                                                                     gg.gCGCTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGt           <  2:1263906/101‑1 (MQ=255)
                                                                                       ggccgccccccccGCCGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGt           <  2:3090657/94‑1 (MQ=18)
                                                                                        gcgcTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTcc         <  1:3685979/101‑1 (MQ=255)
                                                                                        gcgcTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTcc         <  1:380075/101‑1 (MQ=255)
                                                                                          gcTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCg       <  1:2623164/101‑1 (MQ=255)
                                                                                          gcTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCg       <  2:1098549/101‑1 (MQ=255)
                                                                                          gcTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCg       >  1:1019135/1‑101 (MQ=255)
                                                                                           cTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGc      <  1:2171594/101‑1 (MQ=255)
                                                                                           cTGCCCCCGC.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGc      >  2:206730/1‑101 (MQ=255)
                                                                                                   ||                                                                                                
GCGGGTTCGAGCCGACGGAAGAACCGCTTCTGGACTATCTCGAAAGCAAATACGCCGCGATCTACCGGCTTT.GAGGG.C.GA.GGG.GCGCTGCCCCCCG.CGAGGCGATGCCTCGGCCCCCCGGGATATTTCGAGCAAGATGAAAGGCATCCGGTTTCATCTTGCTTCAAATACCTCGGGGGGAGTCCCGCAGGG  >  NC_014034/1740690‑1740880

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG