Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,802,243 2 bp→GC 100% coding (626‑627/2124 nt) ligA → DNA ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,802,2430C→G100.0% 697.7 190T209R (ACG→AGG) ligADNA ligase
Reads supporting (aligned to +/- strand):  new base (92/98):  ref base (0/0):  total (92/98)
*NC_0140341,802,2440G→C100.0% 714.4 190T209T (ACG→ACCligADNA ligase
Reads supporting (aligned to +/- strand):  new base (92/98):  ref base (0/0):  total (92/98)

Only 100 of 192 total aligned reads displayed.

GGCGCCTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGACGTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCCGTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCT  >  NC_014034/1802148‑1802338
                                                                                               ||                                                                                              
 gcgcCTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGc                                                                                           <  1:2328048/101‑1 (MQ=255)
    ccTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCaa                                                                                        <  1:2567189/101‑1 (MQ=255)
     cTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAAt                                                                                       <  1:2274711/101‑1 (MQ=255)
        ccGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCcg                                                                                    >  1:1663787/1‑101 (MQ=255)
          gTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCcgcg                                                                                  >  1:2467920/1‑101 (MQ=255)
           tGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCCTGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCgcgc                                                                                 <  1:4032788/101‑1 (MQ=255)
           tGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCgcgc                                                                                 <  1:4661665/101‑1 (MQ=255)
              tGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAAt                                                                              <  2:592665/101‑1 (MQ=255)
              tGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAAt                                                                              >  1:3627145/1‑101 (MQ=255)
                  aGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATgccg                                                                          <  1:887175/101‑1 (MQ=255)
                   gTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATgccgc                                                                         <  1:68323/101‑1 (MQ=255)
                     ccGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGccgccg                                                                       >  2:3700654/1‑101 (MQ=255)
                        cggcgAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCgg                                                                    <  1:4425879/101‑1 (MQ=255)
                          gcgAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGc                                                                  <  1:3498665/101‑1 (MQ=255)
                          gcgAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGc                                                                  >  1:2024084/1‑101 (MQ=255)
                           cgAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCt                                                                 <  2:935028/101‑1 (MQ=255)
                           cgAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCt                                                                 <  1:2140293/101‑1 (MQ=255)
                             aGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcg                                                               >  1:2952917/1‑100 (MQ=255)
                               gTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcgct                                                             <  1:2872927/101‑4 (MQ=255)
                                tCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcgctg                                                            >  1:47454/1‑97 (MQ=255)
                                tCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcgctg                                                            <  2:3625481/101‑5 (MQ=255)
                                    cATGAGCCATGCCTATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGcc                                                        <  2:2807930/101‑1 (MQ=255)
                                    cATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGcc                                                        >  1:4548166/1‑101 (MQ=255)
                                      tGAGCCATGCCGATTTTNCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAg                                                      <  2:140341/101‑1 (MQ=255)
                                      tGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAg                                                      >  1:2776163/1‑101 (MQ=255)
                                      tGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAg                                                      >  2:2560126/1‑101 (MQ=255)
                                       gAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGc                                                     >  1:2757041/1‑101 (MQ=255)
                                        aGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGTCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCt                                                    <  2:3048472/101‑1 (MQ=255)
                                         gCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTc                                                   <  2:3529889/101‑1 (MQ=255)
                                            aTGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCgcc                                                >  1:1798141/1‑99 (MQ=255)
                                            aTGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGAc                                                <  2:1789468/101‑1 (MQ=255)
                                             tGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACg                                               <  1:2215434/101‑1 (MQ=255)
                                             tGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACg                                               >  2:4478315/1‑101 (MQ=255)
                                                cGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGccc                                            <  1:4611401/101‑1 (MQ=255)
                                                cGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGccc                                            <  2:2912275/101‑1 (MQ=255)
                                                cGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGccc                                            <  2:3488396/101‑1 (MQ=255)
                                                  aTTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGTGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACTCCCgg                                          >  1:4216327/1‑101 (MQ=255)
                                                  aTTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCgg                                          <  1:2425453/101‑1 (MQ=255)
                                                    tttGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGAt                                        >  2:1952112/1‑101 (MQ=255)
                                                    tttGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGAt                                        <  2:3982281/101‑1 (MQ=255)
                                                    tttGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGAt                                        <  1:1546057/101‑1 (MQ=255)
                                                     ttGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATc                                       >  2:546854/1‑101 (MQ=255)
                                                       gcggcgCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATTCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCAc                                     <  2:2280031/101‑1 (MQ=255)
                                                       gcggcgCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCAc                                     <  1:176176/101‑1 (MQ=255)
                                                        ctgcgcTGAATGCACGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCAcc                                    <  2:328446/99‑1 (MQ=255)
                                                           cgcTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGcc                                 <  1:1018447/101‑1 (MQ=255)
                                                           cgcTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGcc                                 <  1:1118830/101‑1 (MQ=255)
                                                           cgcTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGcc                                 >  1:2689805/1‑101 (MQ=255)
                                                           cgcTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGcc                                 >  1:3520230/1‑101 (MQ=255)
                                                            gcTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGccc                                >  1:2237314/1‑100 (MQ=255)
                                                            gcTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCt                                >  1:1743130/1‑101 (MQ=255)
                                                             cTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTc                               <  2:71977/101‑1 (MQ=255)
                                                             cTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTc                               <  1:952740/101‑1 (MQ=255)
                                                             cTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTc                               >  2:1008481/1‑101 (MQ=255)
                                                              tGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTcc                              >  1:3551541/1‑101 (MQ=255)
                                                              tGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTcc                              >  2:2536554/1‑101 (MQ=255)
                                                               gAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCa                             <  2:290533/101‑1 (MQ=255)
                                                               gAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCa                             <  2:2689609/101‑1 (MQ=255)
                                                               gAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCa                             >  2:4269448/1‑101 (MQ=255)
                                                               gAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCa                             <  1:3817104/101‑1 (MQ=255)
                                                                aaTGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAg                            >  2:1820466/1‑101 (MQ=255)
                                                                  tGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGAc                          <  1:1871882/101‑1 (MQ=255)
                                                                   gcgcGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGAcc                         <  1:3700929/101‑1 (MQ=255)
                                                                    cgcgGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGTCAGCTCGACGCCCGGATCACCGCCTCCAGAccc                        >  1:4033697/1‑101 (MQ=255)
                                                                     gcgGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCt                       <  1:4340260/101‑1 (MQ=255)
                                                                     gcgGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCt                       >  2:1283337/1‑101 (MQ=255)
                                                                       ggCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTg                     >  1:3937741/1‑101 (MQ=255)
                                                                        gCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGa                    <  2:47448/101‑1 (MQ=255)
                                                                         cAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGaa                   >  2:3444753/1‑101 (MQ=255)
                                                                          aGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGaaa                  >  2:2646488/1‑101 (MQ=255)
                                                                          aGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGaaa                  >  2:188010/1‑101 (MQ=255)
                                                                           ggCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAAt                 <  1:1314800/101‑1 (MQ=255)
                                                                           ggCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAAt                 <  2:4017096/101‑1 (MQ=255)
                                                                           ggCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAAt                 <  2:1492138/101‑1 (MQ=255)
                                                                           ggCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAAt                 >  1:2014846/1‑101 (MQ=255)
                                                                           ggCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAAt                 >  2:4472572/1‑101 (MQ=255)
                                                                            gCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAAtt                <  2:1326588/101‑1 (MQ=255)
                                                                            gCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAAtt                >  2:386170/1‑101 (MQ=255)
                                                                            gCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAAtt                <  2:2947433/101‑1 (MQ=255)
                                                                              ggAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCt              <  1:3266308/101‑1 (MQ=255)
                                                                               gAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCTAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCtt             <  1:3991945/101‑1 (MQ=255)
                                                                               gAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCtt             <  2:3721948/101‑1 (MQ=255)
                                                                               gAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCtt             >  2:2260582/1‑101 (MQ=255)
                                                                                aGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCttt            <  1:628314/101‑1 (MQ=255)
                                                                                aGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCttt            <  2:2844765/101‑1 (MQ=255)
                                                                                 gCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTg           <  2:4547871/101‑1 (MQ=255)
                                                                                   ggggCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGcc         <  2:2775965/101‑1 (MQ=255)
                                                                                   ggggCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGcc         <  2:2025907/101‑1 (MQ=255)
                                                                                   ggggCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGcc         >  1:3782058/1‑101 (MQ=255)
                                                                                    gggCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCt        >  1:2822271/1‑101 (MQ=255)
                                                                                    gggCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCt        <  2:1218865/101‑1 (MQ=255)
                                                                                     ggCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTa       <  2:1867023/101‑1 (MQ=255)
                                                                                      gCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTAt      <  1:2910276/101‑1 (MQ=255)
                                                                                       cGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTAtt     <  1:4621227/101‑1 (MQ=255)
                                                                                       cGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTAtt     >  1:2484110/1‑101 (MQ=255)
                                                                                       cGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTAtt     <  2:2952688/101‑1 (MQ=255)
                                                                                        gACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTc    >  2:1416781/1‑101 (MQ=255)
                                                                                        gACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTc    <  2:2017157/101‑1 (MQ=255)
                                                                                        gACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTc    >  2:3296611/1‑101 (MQ=255)
                                                                                          cAAGAGCTTCGCCAAGCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCt  <  2:415609/101‑1 (MQ=255)
                                                                                               ||                                                                                              
GGCGCCTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGACGTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCCGTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCT  >  NC_014034/1802148‑1802338

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG