Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,950,871 2 bp→GC 100% coding (855‑856/873 nt) garR → 2‑hydroxy‑3‑oxopropionate reductase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,950,8710C→G100.0% 723.4 195R285R (CGC→CGGgarR2‑hydroxy‑3‑oxopropionate reductase
Reads supporting (aligned to +/- strand):  new base (95/100):  ref base (0/0):  total (95/100)
*NC_0140341,950,8720G→C100.0% 714.9 194M286M (GTG→CTG) garR2‑hydroxy‑3‑oxopropionate reductase
Reads supporting (aligned to +/- strand):  new base (94/100):  ref base (0/0):  total (94/100)

Only 100 of 203 total aligned reads displayed.

CAGCCTGCCGGTCACCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGCGTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCCG.TTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGTTTTCCT  >  NC_014034/1950778‑1950965
                                                                                             ||                                                                                              
cAGCCTGGCGGTCACCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTgcgcg                                                                                          <  1:1172015/101‑1 (MQ=255)
cAGCCTGCCGGTCACCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTgcgcg                                                                                          >  2:3662585/1‑101 (MQ=255)
     tGCCGGTCACCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTc                                                                                     >  2:2405300/1‑101 (MQ=255)
     tGCCGGTCACCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTc                                                                                     >  2:407913/1‑101 (MQ=255)
     tGCCGGTCACCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTc                                                                                     <  1:532836/101‑1 (MQ=255)
     tGCCGGTCACCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTc                                                                                     >  2:2374505/1‑101 (MQ=255)
      gCCGGTCACCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCg                                                                                    >  1:1462672/1‑101 (MQ=255)
      gCCGGTCACCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCg                                                                                    >  2:4283196/1‑101 (MQ=255)
       ccGGTCACCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCgg                                                                                   <  2:1502029/101‑1 (MQ=255)
       ccGGTCACCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCgg                                                                                   >  2:4197286/1‑101 (MQ=255)
         ggTCACCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCt                                                                                 >  1:2490164/1‑101 (MQ=255)
            cACCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAg                                                                              >  1:67715/1‑101 (MQ=255)
             aCCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGTGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGc                                                                             <  1:1901302/101‑1 (MQ=255)
             aCCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGc                                                                             >  2:1234372/1‑101 (MQ=255)
             aCCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGc                                                                             <  1:2107973/101‑1 (MQ=255)
               cgcgCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCgg                                                                           <  2:4377353/101‑1 (MQ=255)
                 cgcTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGa                                                                         >  2:1590248/1‑101 (MQ=255)
                  gcTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAg                                                                        <  1:940621/101‑1 (MQ=255)
                  gcTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAg                                                                        <  1:795513/101‑1 (MQ=255)
                  gcTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAg                                                                        >  2:4272085/1‑101 (MQ=255)
                  gcTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAg                                                                        >  1:278401/1‑101 (MQ=255)
                  gcTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAg                                                                        <  1:1386275/101‑1 (MQ=255)
                     ggTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAgggg                                                                     >  1:2268834/1‑101 (MQ=255)
                     ggTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAgggg                                                                     >  2:3282643/1‑101 (MQ=255)
                      gTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAggggg                                                                    <  2:1069939/101‑1 (MQ=255)
                       tCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAgggggg                                                                   >  1:3222091/1‑101 (MQ=255)
                        cGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGgc                                                                  <  2:4528613/101‑1 (MQ=255)
                         gATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGgcg                                                                 >  2:2769094/1‑101 (MQ=255)
                          aTCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGgcgc                                                                >  2:2620133/1‑101 (MQ=255)
                          aTCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGgagc                                                                >  2:3983110/1‑101 (MQ=255)
                           tCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCt                                                               >  2:1631543/1‑101 (MQ=255)
                             aGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGc                                                             >  2:1835214/1‑101 (MQ=255)
                             aGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGc                                                             >  2:4234671/1‑101 (MQ=255)
                                  tACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGcccccg                                                        >  1:2802368/1‑100 (MQ=255)
                                    cAAGGATGTGCAGAAGCTGGGCGGCGGGTCCTGGGACACCTGGAGCCTGATCGGGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGcccccggc                                                      <  1:2728549/101‑4 (MQ=255)
                                    cAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGcccccggc                                                      >  2:3498131/1‑98 (MQ=255)
                                      aGGATGTGCAGAAGCTGGGCGGGGGGCGCTGGGACACCCCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGcccccggctt                                                    <  2:3816234/101‑6 (MQ=255)
                                      aGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGcccccggctt                                                    >  1:588601/1‑96 (MQ=255)
                                       ggATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGTGAGGGGGGCGCTGcccccggcttc                                                   >  1:1257871/1‑95 (MQ=255)
                                        gATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGcccccggcttcg                                                  <  2:1890416/101‑8 (MQ=255)
                                            tgCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGcccc                                              <  2:588540/101‑1 (MQ=255)
                                            tgCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGcccc                                              >  2:4007842/1‑101 (MQ=255)
                                             gCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGccccc                                             >  1:188245/1‑101 (MQ=255)
                                              cAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGcccccc                                            >  2:1111448/1‑101 (MQ=255)
                                               aGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGccccccc                                           >  2:3402164/1‑101 (MQ=255)
                                               aGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGccccccc                                           >  2:444399/1‑101 (MQ=255)
                                                gAATCTGGGGAGGGGGCGCTGGGACACCGCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCg                                          <  2:3688270/101‑1 (MQ=255)
                                                gAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGGGCTGCCCCCGGCTTCGCCCCCCCg                                          >  2:3900910/1‑101 (MQ=255)
                                                gAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGGGCTGCCCCCCGCCTCCCCCCCCCg                                          >  1:384292/1‑101 (MQ=255)
                                                gAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCg                                          <  2:1150132/101‑1 (MQ=255)
                                                gAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCg                                          <  2:2905950/101‑1 (MQ=255)
                                                 aaGCTGGGCGGCGGGCGCTGGGACCCCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCgg                                         <  1:509529/101‑1 (MQ=255)
                                                  aGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCggg                                        <  2:644053/101‑1 (MQ=255)
                                                  aGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCggg                                        >  2:213199/1‑101 (MQ=255)
                                                   gCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGa                                       <  1:2020415/101‑1 (MQ=255)
                                                    cTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGat                                      <  1:1111537/101‑1 (MQ=255)
                                                    cTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGat                                      <  1:2405462/101‑1 (MQ=255)
                                                    cTGGGCGGCGGGCGATGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGat                                      <  1:4026758/101‑1 (MQ=255)
                                                      gggCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGatat                                    >  1:4039220/1‑101 (MQ=255)
                                                      gggCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGatat                                    >  2:2060687/1‑101 (MQ=255)
                                                        gcggcgGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATAttt                                  <  1:4093848/101‑1 (MQ=38)
                                                        gcggcgGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATAttt                                  <  2:4221869/101‑1 (MQ=38)
                                                        gcggcgGGCGCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATAttt                                  <  1:2528139/101‑1 (MQ=38)
                                                            cgggcgCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGccc                              <  1:4488767/101‑1 (MQ=38)
                                                              ggcgCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCa                            <  1:869136/101‑1 (MQ=38)
                                                              ggcgCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCa                            <  1:4272373/101‑1 (MQ=38)
                                                              ggcgCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCa                            <  1:2452611/101‑1 (MQ=38)
                                                              ggcgCTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCa                            <  1:1590353/101‑1 (MQ=38)
                                                              ggcgATGGGACCCCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCa                            <  1:3365927/101‑1 (MQ=37)
                                                                cgcTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAg                          >  2:2256775/1‑101 (MQ=38)
                                                                 ccTGGGACACCGCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAgg                         <  2:3022244/100‑1 (MQ=38)
                                                                  cTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGt                        <  2:4323789/101‑1 (MQ=38)
                                                                  cTGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGt                        <  1:3061398/101‑1 (MQ=38)
                                                                   tGGGACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTg                       >  1:2170219/1‑101 (MQ=38)
                                                                     ggACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGaa                     <  1:1685233/101‑1 (MQ=38)
                                                                      gACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGaaa                    <  2:3277387/101‑1 (MQ=38)
                                                                      gACACCTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGaaa                    <  1:1835340/101‑1 (MQ=38)
                                                                         cccTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGcc                 <  2:822909/100‑1 (MQ=38)
                                                                         cccTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGcc                 <  2:133188/100‑1 (MQ=38)
                                                                         cccTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGcc                 <  1:2206594/100‑1 (MQ=38)
                                                                          ccTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCcg                >  1:1059341/1‑101 (MQ=37)
                                                                          ccTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCcg                <  2:636999/101‑1 (MQ=38)
                                                                          ccTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCcg                >  2:1434970/1‑101 (MQ=37)
                                                                           cTCGAGCCTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCcgc               <  1:2060827/101‑1 (MQ=38)
                                                                                 gcTGATCGCGCGGCTGCGCGGGCTCGGCTGGGAGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTTCCCCAAGGTGAAAGCCGCGACGAg         <  1:4541834/100‑1 (MQ=25)
                                                                                 ccTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAg         <  1:3282888/101‑1 (MQ=38)
                                                                                  cTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGt        >  1:1707632/1‑101 (MQ=38)
                                                                                  cTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGt        <  1:3890356/101‑1 (MQ=38)
                                                                                  cTGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGt        <  1:3597398/101‑1 (MQ=38)
                                                                                   tGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGtt       >  1:1034267/1‑101 (MQ=38)
                                                                                   tGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGtt       <  1:3709804/101‑1 (MQ=38)
                                                                                   tGATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGtt       >  2:3631007/1‑101 (MQ=38)
                                                                                    gATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGttt      <  1:3336103/101‑1 (MQ=38)
                                                                                    gATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGttt      <  1:4285331/101‑1 (MQ=38)
                                                                                    gATCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGttt      <  2:67709/101‑1 (MQ=38)
                                                                                     aTCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGtttt     >  2:46794/1‑101 (MQ=38)
                                                                                     aTCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGtttt     >  2:3855744/1‑101 (MQ=38)
                                                                                     aTCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGtttt     >  2:2480009/1‑101 (MQ=38)
                                                                                      tCGCGCGGCTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGTTTTc    <  1:4451898/101‑1 (MQ=38)
                                                                                       cgcgcggcTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCGGCTTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGTTTTcc   <  1:134600/101‑1 (MQ=38)
                                                                                             ||                                                                                              
CAGCCTGCCGGTCACCGCGCTGGTCGATCAGTTCTACAAGGATGTGCAGAAGCTGGGCGGCGGGCGCTGGGACACCTCGAGCCTGATCGCGCGCGTGCGCGCGCTCGGCTGAGCGGGAGGGGGGCGCTGCCCCCCG.TTCGCCCCCCCGGGATATTTGCCCCAAGGTGAAAGCCGCGACGAGTTTTCCT  >  NC_014034/1950778‑1950965

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG