Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,289 2 bp→AT 100% coding (87‑88/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,2890T→A100.0% 674.8 175T30S (ACA→TCA) rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (93/82):  ref base (0/0):  total (93/82)
*NC_0140342,177,2900A→T100.0% 671.0 173C29* (TGT→TGArbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (91/82):  ref base (0/0):  total (91/82)

Only 100 of 180 total aligned reads displayed.

CATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..GGGTTG..GT..CGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATC  >  NC_014034/2177202‑2177374
                                                                                              ||                                                                                        
 aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAgg                                                                                  <  2:2097821/101‑1 (MQ=255)
  tAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGc                                                                                 <  1:1134447/101‑1 (MQ=255)
     gTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGcccc                                                                              >  1:3625745/1‑101 (MQ=255)
     gTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGcccc                                                                              <  2:3917758/101‑1 (MQ=255)
       cGAGAGCACCAGCCCCGTCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGGCAGGCCCCgg                                                                            >  2:4097078/1‑101 (MQ=255)
       cGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCgg                                                                            >  2:1885011/1‑101 (MQ=255)
        gAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGt                                                                           >  2:893827/1‑101 (MQ=255)
        gAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGt                                                                           >  2:1288617/1‑101 (MQ=255)
         agagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGtt                                                                          <  1:4532225/98‑1 (MQ=255)
          gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..TGGTCTTGGTGATCAGACTGGCCCCGGttt                                                                         <  2:2039335/99‑1 (MQ=255)
          gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGttt                                                                         <  2:2490345/99‑1 (MQ=255)
          gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGttt                                                                         >  2:1816124/3‑101 (MQ=255)
          gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGttt                                                                         <  2:3523721/99‑1 (MQ=255)
          gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGttt                                                                         >  1:1344486/3‑101 (MQ=255)
            gCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCg                                                                       <  1:2376930/101‑1 (MQ=255)
              aCCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGc                                                                     <  2:2257341/101‑1 (MQ=255)
               ccAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGcc                                                                    >  1:3312310/1‑101 (MQ=255)
                cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCg                                                                   <  2:580900/101‑1 (MQ=255)
                 aGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.a                                                                 <  1:222162/101‑1 (MQ=255)
                   ccccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aaa                                                               >  2:3290119/1‑99 (MQ=255)
                    cccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagc                                                              >  1:876508/1‑98 (MQ=255)
                    cccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagc                                                              >  2:352001/1‑98 (MQ=255)
                     ccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagcc                                                             <  1:2435819/101‑5 (MQ=255)
                     ccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagcc                                                             <  2:2257631/101‑5 (MQ=255)
                     ccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagcc                                                             <  2:1182897/101‑5 (MQ=255)
                      cATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagccg                                                            <  1:1053381/101‑6 (MQ=255)
                      cATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagccg                                                            >  2:1441184/1‑96 (MQ=255)
                         ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGccc                                                          >  2:3317274/1‑101 (MQ=255)
                          tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGcccc                                                         <  2:3498720/101‑1 (MQ=255)
                          tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGcccc                                                         >  1:332814/1‑101 (MQ=255)
                           cGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCg                                                        >  2:1395632/1‑101 (MQ=255)
                           cGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCg                                                        >  1:4286492/1‑101 (MQ=255)
                            ggTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCgg                                                       <  1:352033/101‑1 (MQ=255)
                            ggTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCgg                                                       >  2:3601073/1‑101 (MQ=255)
                             gTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGc                                                      >  2:83239/1‑101 (MQ=255)
                             gTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGc                                                      >  1:4429419/1‑101 (MQ=255)
                              tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                                                     <  1:1512349/101‑1 (MQ=255)
                               ggCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                    <  1:3120602/100‑1 (MQ=255)
                               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                    <  2:2623498/101‑1 (MQ=255)
                               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                    >  2:3549936/1‑101 (MQ=255)
                                gCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAg                                                   <  1:3495234/101‑1 (MQ=255)
                                gCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAg                                                   <  1:4117049/101‑1 (MQ=255)
                                 ccTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgc                                                  >  1:2876285/1‑101 (MQ=255)
                                 ccTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgc                                                  <  2:3971190/101‑1 (MQ=255)
                                 ccTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgc                                                  <  1:1870166/101‑1 (MQ=255)
                                  cTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGGTTCGGCCGAAGCCGCCCCGGCCAgcg                                                 >  1:3918233/1‑101 (MQ=255)
                                   tttGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcgc                                                <  2:3155/101‑1 (MQ=255)
                                   tttGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcgc                                                >  1:3531667/1‑101 (MQ=255)
                                    ttGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGcgcg                                               <  2:1427927/101‑1 (MQ=255)
                                     tGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                                              <  1:2529128/101‑1 (MQ=255)
                                      gCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGa                                             >  2:620292/1‑101 (MQ=255)
                                      gCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGa                                             <  1:72483/101‑1 (MQ=255)
                                      gCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGa                                             <  1:2441702/101‑1 (MQ=255)
                                       ccGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                                            <  2:107625/101‑1 (MQ=255)
                                       ccGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                                            <  1:666329/101‑1 (MQ=255)
                                       ccGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTCATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                                            >  1:1572506/1‑101 (MQ=255)
                                        cGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                                           >  2:2353656/1‑101 (MQ=255)
                                        cGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                                           >  2:2390484/1‑101 (MQ=255)
                                        cGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                                           >  2:1586815/1‑101 (MQ=255)
                                         gAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACCGGCCCCGGCTTCGGCCGAAGCCGGCCCGGCCAGCGCCGacaa                                          >  1:128843/1‑101 (MQ=255)
                                         gAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaaa                                          <  2:4567813/101‑1 (MQ=255)
                                         gAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaaa                                          <  2:4004975/101‑1 (MQ=255)
                                          aaGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                                         <  1:4606522/101‑1 (MQ=255)
                                           aggaCCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACg                                        <  1:3849646/98‑1 (MQ=255)
                                              aCCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                     <  2:535089/101‑1 (MQ=255)
                                               ccttccttCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.g                                   <  2:1535063/101‑1 (MQ=255)
                                               ccttccttCATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.g                                   <  1:486626/101‑1 (MQ=255)
                                                cttccttcATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                  <  2:4380081/101‑1 (MQ=255)
                                                cttccttcATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                  >  2:4638462/1‑101 (MQ=255)
                                                 ttccttcATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.ggg                                 >  1:4223900/1‑100 (MQ=255)
                                                    cttcATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcag                              >  1:3025176/1‑97 (MQ=255)
                                                     ttcATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                             >  1:1039340/1‑96 (MQ=255)
                                                     ttcATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                             >  1:434106/1‑96 (MQ=255)
                                                     ttcATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaag                             >  1:3373636/1‑96 (MQ=255)
                                                      tcATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                             >  1:1376870/1‑101 (MQ=255)
                                                      tcATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                             <  1:3460977/101‑1 (MQ=255)
                                                      tcATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                             >  1:3597438/1‑101 (MQ=255)
                                                      tcATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                             >  1:4445006/1‑101 (MQ=255)
                                                       cATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                            <  1:987794/101‑1 (MQ=255)
                                                       cATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                            >  1:1273251/1‑101 (MQ=255)
                                                       cATCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                            >  2:2474680/1‑101 (MQ=255)
                                                        aTCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCaagatcggaag                           >  2:3382245/1‑91 (MQ=255)
                                                         tCTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc                         <  1:2808819/101‑1 (MQ=255)
                                                         tCTTGACGAAAAAGGGGTTG..GTGGCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cc                         >  2:1827423/1‑101 (MQ=255)
                                                          cTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.ccc                        >  2:4497451/1‑101 (MQ=255)
                                                          cTTGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.ccc                        >  1:2881723/1‑101 (MQ=255)
                                                           ttGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cccc                       <  1:3265855/101‑1 (MQ=255)
                                                           ttGACGAAAAAGGGGTTGGTGT..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cacc                       >  2:3933967/1‑101 (MQ=255)
                                                                         gttggtgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tt         <  2:1949770/95‑1 (MQ=255)
                                                                          ttggtgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.ttt        <  1:620353/96‑2 (MQ=255)
                                                                           tggtgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg       >  2:3213533/5‑99 (MQ=255)
                                                                           tggtgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg       >  1:2588294/5‑99 (MQ=255)
                                                                           tggtgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg       <  2:1039257/97‑3 (MQ=255)
                                                                            ggtgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc      >  2:2731979/4‑98 (MQ=255)
                                                                            ggtgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc      >  2:2532896/4‑98 (MQ=255)
                                                                              tgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcgc    >  2:356407/2‑96 (MQ=255)
                                                                              tgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcgc    >  1:1645370/2‑96 (MQ=255)
                                                                              tgt..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcgc    >  2:667951/2‑96 (MQ=255)
                                                                                t..cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcat   <  1:1809581/101‑1 (MQ=255)
                                                                                   cggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcatc  >  1:2049178/1‑101 (MQ=255)
                                                                                              ||                                                                                        
CATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..GGGTTG..GT..CGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATC  >  NC_014034/2177202‑2177374

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG