Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,180,185 2 bp→GC 100% coding (874‑875/1314 nt) RCAP_rcc02022 → polyols ABC transporter periplasmic polyols‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,180,1850C→G100.0% 730.1 198R292G (CGC→GGC) RCAP_rcc02022polyols ABC transporter periplasmic polyols‑binding protein
Reads supporting (aligned to +/- strand):  new base (90/107):  ref base (0/1):  total (90/108)
*NC_0140342,180,1860G→C100.0% 747.5 199R292P (CGC→CCC) RCAP_rcc02022polyols ABC transporter periplasmic polyols‑binding protein
Reads supporting (aligned to +/- strand):  new base (90/109):  ref base (0/0):  total (90/109)

Only 100 of 202 total aligned reads displayed.

ATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCTGGC.CCGAAC.AAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAC  >  NC_014034/2180093‑2180282
                                                                                                ||                                                                                                
aTGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAg                                                                                              <  2:1952431/101‑1 (MQ=255)
aTGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAg                                                                                              <  2:2875913/101‑1 (MQ=255)
aTGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAg                                                                                              >  2:2935105/1‑101 (MQ=255)
 tGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGc                                                                                             <  2:3320372/101‑1 (MQ=255)
  gCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCa                                                                                            >  2:1001375/1‑101 (MQ=255)
   cGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAATCAt                                                                                           <  2:146445/101‑1 (MQ=255)
   cGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCAt                                                                                           <  1:2550459/101‑1 (MQ=255)
    gACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATg                                                                                          <  1:3157243/101‑1 (MQ=255)
    gACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATg                                                                                          <  1:1510445/101‑1 (MQ=255)
    gACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCCGGCGCCGAAC.AAGGCCGGTGGCGCCAAGCATg                                                                                          >  1:556369/1‑101 (MQ=255)
         tGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAAc                                                                                     >  1:3548042/1‑101 (MQ=255)
          ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACt                                                                                    <  1:2237335/101‑1 (MQ=255)
           gccgccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTg                                                                                   <  2:13138/101‑1 (MQ=255)
            ccgccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTgg                                                                                  <  2:893810/101‑1 (MQ=255)
              gccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGct                                                                                >  1:862823/1‑101 (MQ=255)
               ccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGctc                                                                               >  1:674608/1‑101 (MQ=255)
                ctctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGATTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGctct                                                                              >  2:3372125/1‑101 (MQ=255)
                ctatttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGctct                                                                              <  2:3929736/98‑1 (MQ=255)
                 tctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTg                                                                             >  2:3061688/1‑101 (MQ=255)
                 tctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTg                                                                             <  1:139785/101‑1 (MQ=255)
                 tctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACAGGCTCTg                                                                             >  2:4302270/1‑101 (MQ=255)
                  ctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTgg                                                                            >  2:4088544/1‑101 (MQ=255)
                   tttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTggg                                                                           >  2:29788/1‑101 (MQ=255)
                   tttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTggg                                                                           >  2:958512/1‑101 (MQ=255)
                    ttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAAGC.CGGTGTCGCCAAGCATGGCAACTGGCTCTGGGc                                                                          >  2:740661/1‑101 (MQ=255)
                     tCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGcc                                                                         >  1:3530140/1‑101 (MQ=255)
                       gTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTg                                                                       <  2:2461370/101‑1 (MQ=255)
                       gTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTg                                                                       <  1:3670101/101‑1 (MQ=255)
                        tGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTgg                                                                      <  1:3047842/101‑1 (MQ=255)
                        tGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTgg                                                                      >  2:192300/1‑101 (MQ=255)
                         gACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGa                                                                     >  1:3786385/1‑101 (MQ=255)
                         gACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGa                                                                     >  1:1126035/1‑101 (MQ=255)
                          aCCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGaa                                                                    <  1:4520109/101‑1 (MQ=255)
                           ccAAGCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAAGTGGCTCTGGGCCGGGAAc                                                                   <  2:229305/101‑1 (MQ=255)
                           ccAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAc                                                                   <  1:2381351/101‑1 (MQ=255)
                            cAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcc                                                                  <  1:4206862/101‑1 (MQ=255)
                            cAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcc                                                                  <  2:3956242/101‑1 (MQ=255)
                             caCCCCAAGGGCCCCGCCGGGGGCGATCCTGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcct                                                                 <  1:259302/100‑2 (MQ=255)
                              aCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctc                                                                <  2:4230445/101‑3 (MQ=255)
                              aCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctc                                                                <  2:2225738/101‑3 (MQ=255)
                                 ccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGcc                                                            <  2:3811497/101‑1 (MQ=255)
                                  cAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCa                                                           <  2:3850218/101‑1 (MQ=255)
                                   aaGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAAATGGCTCTGGGCCTGGAA.CCTCGCCat                                                          >  1:3914950/1‑100 (MQ=255)
                                    aGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCCCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCatt                                                         <  1:4302559/101‑3 (MQ=255)
                                     ggACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattc                                                        <  1:1001458/101‑4 (MQ=255)
                                     ggACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCCGGCGCCGAAC.AAGGCCGGGGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattc                                                        >  1:463061/1‑98 (MQ=255)
                                      gACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattcc                                                       <  1:1420202/101‑5 (MQ=255)
                                        cTCGGCCGTGGCCGATCATTTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcgg                                                    >  1:1905480/1‑101 (MQ=255)
                                        cTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcgg                                                    >  2:269498/1‑101 (MQ=255)
                                          cGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggcg                                                  <  1:1563345/101‑1 (MQ=255)
                                            gCCGTGGCCGATCATGTAGGCTTCGCGCTGGCGCCGAAC.AAGGCCCGCGTCGCCAAGCATGGCAACTGGGTCTGGGCCTGG.ACCCTCGCC.ATTCCGGCggg                                                >  2:3101666/1‑101 (MQ=255)
                                             ccGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGc                                               <  1:2163702/101‑1 (MQ=255)
                                              cGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCa                                              <  1:4439364/101‑1 (MQ=255)
                                              cGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCa                                              >  2:649540/1‑101 (MQ=255)
                                                tGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACg                                            >  1:120355/1‑101 (MQ=255)
                                                 ggCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGc                                           >  1:768514/1‑101 (MQ=255)
                                                  gCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCa                                          <  2:3237863/101‑1 (MQ=255)
                                                  gCCGATCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCa                                          <  1:1945081/101‑1 (MQ=255)
                                                      aTCATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCATGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaag                                      >  1:4006217/1‑100 (MQ=255)
                                                        cATGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggc                                    >  1:1326815/1‑98 (MQ=255)
                                                          tGTCGGCTTCGCGCTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCg                                 >  1:4428347/1‑101 (MQ=255)
                                                            tcggcttcgcgcTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAg                               <  1:1380101/101‑1 (MQ=255)
                                                              ggcttcgcgcTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc                             >  1:2089583/1‑101 (MQ=255)
                                                               gcttcgcgcTGGCGCCGATC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcg                            <  2:4385183/101‑1 (MQ=255)
                                                               gcttcgcgcTGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcg                            <  2:1778867/101‑1 (MQ=255)
                                                                cttcgcgctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcgg                           <  1:2706586/95‑1 (MQ=255)
                                                                  tcgcgctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGgcggcg                         <  1:2935334/97‑1 (MQ=255)
                                                                  tcgcgctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGgcggcg                         >  1:2536543/5‑101 (MQ=255)
                                                                    gcgctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCt                       <  1:3197371/99‑1 (MQ=255)
                                                                    gcgctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCt                       >  2:2166186/3‑101 (MQ=255)
                                                                    gcgctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCt                       <  2:831617/99‑1 (MQ=255)
                                                                    gcgctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCt                       <  2:1293698/99‑1 (MQ=255)
                                                                     cgctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCNGCGGGCACGC.AAAAGGCCGAGGCGGCGCTg                      >  1:965467/2‑101 (MQ=255)
                                                                     cgctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTg                      <  2:3716341/100‑1 (MQ=255)
                                                                     cgctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTg                      <  2:963268/100‑1 (MQ=255)
                                                                     cgctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTg                      <  2:1013234/100‑1 (MQ=255)
                                                                      gctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGa                     >  1:3314842/1‑101 (MQ=255)
                                                                      gctGGCGCCGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGa                     >  1:1280689/1‑101 (MQ=255)
                                                                        tggcgccGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaaa                   <  1:1012680/96‑1 (MQ=255)
                                                                         ggcgccGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAt                  >  1:3821779/5‑101 (MQ=255)
                                                                         ggcgccGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAt                  >  1:3092119/5‑101 (MQ=255)
                                                                         ggcgccGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAt                  <  2:1612462/97‑1 (MQ=255)
                                                                         ggcgccGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAt                  >  2:4535069/5‑101 (MQ=255)
                                                                          gcgccGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAtt                 <  1:706751/98‑1 (MQ=255)
                                                                           cgccGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtc                <  1:3869176/99‑1 (MQ=255)
                                                                            gccGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcg               >  1:3884854/2‑101 (MQ=255)
                                                                            gccGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcg               <  2:207291/100‑1 (MQ=255)
                                                                             ccGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgt              >  2:519131/1‑101 (MQ=255)
                                                                              cGAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtc             >  2:2599502/1‑101 (MQ=255)
                                                                               gAAC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtcg            <  2:1125946/101‑1 (MQ=255)
                                                                                 aC.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGaa          <  1:1684928/101‑1 (MQ=255)
                                                                                  c.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAt         >  2:4356357/1‑101 (MQ=255)
                                                                                  c.AAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAt         <  2:3723560/101‑1 (MQ=255)
                                                                                    aaggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATg        >  1:4581558/6‑101 (MQ=255)
                                                                                    aaggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATg        <  1:1295106/96‑1 (MQ=255)
                                                                                     aggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATgg       <  2:3504447/97‑1 (MQ=255)
                                                                                      gcccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATggg      <  1:4343855/98‑1 (MQ=255)
                                                                                        ccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCg    <  1:33806/100‑1 (MQ=255)
                                                                                         cGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGa   <  2:1846814/101‑1 (MQ=255)
                                                                                         cGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGACGAGGCGGCGCTGAAATTCGTCGAATGGGCGa   >  2:4337084/1‑101 (MQ=255)
                                                                                                ||                                                                                                
ATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCTGGC.CCGAAC.AAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAC  >  NC_014034/2180093‑2180282

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG