Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,747,876	2 bp→GC	100%	intergenic (‑208/+84)	RCAP_rcc02553 ← / ← RCAP_rcc02554	hypothetical protein/NmrA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,747,876	0	C→G	100.0%	56.7	22	intergenic (‑208/+85)	RCAP_rcc02553/RCAP_rcc02554	hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (19/3):  ref base (0/0):  total (19/3)
*	NC_014034	2,747,877	0	T→C	100.0%	78.2	28	intergenic (‑209/+84)	RCAP_rcc02553/RCAP_rcc02554	hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (25/3):  ref base (0/0):  total (25/3)

GCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.C.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCATGCCAGC  >  NC_014034/2747786‑2747970                                                                                              ||                                                                                              gcccgcAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGCAGCGCCCGCCGGGCCGGGCGCTGccc                                                                                        >  1:2730801/1‑101 (MQ=21)     gcAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGCgg                                                                                    >  2:17465/1‑101 (MQ=21)     cccgcgcCCGGTGCCGGCATGGCGGCTTCAGCCCGCACCACAGAGGGCAGCGCTGGGCCGG.GCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAgg                                                                                    <  2:201514/98‑1 (MQ=12)                                                           cggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGCCACAGGACCTCTGTGACGCATGGcagaacggaag                              >  1:748448/1‑91 (MQ=17)                                                            ggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCCTGGTCAAGGCGAtcg                             >  2:1929585/1‑99 (MQ=18)                                                            ggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg                             >  1:2973239/1‑101 (MQ=18)                                                              gcccggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGc                            >  2:3444205/6‑101 (MQ=18)                                                              gcccggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGc                            >  1:2874694/6‑101 (MQ=18)                                                                ggggggggcGAGACGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCt                          <  2:2730603/93‑1 (MQ=21)                                                                ccggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCt                          >  2:1544804/4‑101 (MQ=21)                                                                 cggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCtt                         >  1:4234037/3‑101 (MQ=21)                                                                  ggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTg                        >  2:789648/2‑101 (MQ=21)                                                                  ggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTg                        >  1:254778/2‑101 (MQ=21)                                                                    cgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGc                      >  1:3710024/1‑101 (MQ=21)                                                                     gggcGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCCTGGCCAAGGCGATGGCCTTGGcc                     >  2:4117680/4‑101 (MQ=18)                                                                        cGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTTGGCCCTCGGGGGGCACAGGACCTCTATGACCCATGGGCAAGGCGGTGGCCTCGGCCCAg                  >  1:4029713/1‑101 (MQ=18)                                                                         gagaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAgg                 >  1:2615270/1‑101 (MQ=21)                                                                         gagaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAgg                 >  1:193052/1‑101 (MQ=21)                                                                          agaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGt                >  1:3509186/1‑101 (MQ=21)                                                                          agaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGt                >  2:3340861/1‑101 (MQ=21)                                                                          agaAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGAATGGCCAAGGCGATGGCCTTGGCCAAGGt                >  2:3373281/1‑101 (MQ=21)                                                                            aaGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCa              >  1:842795/1‑101 (MQ=21)                                                                                gcccgccgGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTc          <  2:254754/101‑1 (MQ=21)                                                                                    gccgggccgggCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCATGc      >  1:3570452/5‑101 (MQ=255)                                                                                      cgggccgggCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCATGCCa    >  2:388862/3‑101 (MQ=255)                                                                                       gggccgggCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCATGCCAg   >  1:328057/2‑101 (MQ=255)                                                                                       gggccgggCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCATGCCAg   >  2:3831691/2‑101 (MQ=255)                                                                                        ggccgggCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCATGCCAgc  >  1:2805450/1‑101 (MQ=255)                                                                                        ggccgggCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGGCAGCTCATGCCAgc  >  1:932666/1‑101 (MQ=255)                                                                                              ||                                                                                              GCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.C.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCATGCCAGC  >  NC_014034/2747786‑2747970 Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG