Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,394,235 2 bp→GC 100% coding (787‑788/831 nt) RCAP_rcc03194 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,394,2350C→G100.0% 705.3 191R263P (CGG→CCG) RCAP_rcc03194hypothetical protein
Reads supporting (aligned to +/- strand):  new base (110/80):  ref base (0/1):  total (110/81)
*NC_0140343,394,2360G→C100.0% 727.1 192R263G (CGG→GGG) RCAP_rcc03194hypothetical protein
Reads supporting (aligned to +/- strand):  new base (110/81):  ref base (0/1):  total (110/82)

Only 100 of 208 total aligned reads displayed.

TCGGGCGGGACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCCG.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCCGACCCAGCGTGCCAAGCGCCTTGCCCAGTTCC  >  NC_014034/3394140‑3394330
                                                                                               ||                                                                                               
tCGGGCGGGACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCt                                                                                            >  1:203371/1‑101 (MQ=255)
tCGGGCGGGACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCt                                                                                            >  2:1312057/1‑101 (MQ=255)
  gggcgggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTc                                                                                          <  2:3196218/101‑1 (MQ=255)
  gggcgggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTc                                                                                          >  2:4160675/1‑101 (MQ=255)
   ggcgggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCCCCTTGCCCGC.CGCTTcc                                                                                         >  2:2239155/1‑101 (MQ=255)
     cgggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGa                                                                                       >  1:3368779/1‑101 (MQ=255)
      gggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAg                                                                                      >  2:4557211/1‑101 (MQ=255)
      gggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAg                                                                                      >  1:1864970/1‑101 (MQ=255)
      gggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAg                                                                                      >  2:440569/1‑101 (MQ=255)
      gggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAg                                                                                      >  2:2782147/1‑101 (MQ=255)
        gACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGcc                                                                                    >  2:489602/1‑101 (MQ=255)
           cTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCggg                                                                                 >  2:1623435/1‑101 (MQ=255)
             ccccGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGaa                                                                               >  1:948598/1‑101 (MQ=255)
             ccccGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGaa                                                                               >  1:2472538/1‑101 (MQ=255)
              cccGCGCAGCAGGGCCGCGCGCGGAGCGTCAGGGTCGGCCACGGGCAGCCAGGCGCCGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGaaa                                                                              <  1:2632344/101‑1 (MQ=255)
               ccGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCCCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAAc                                                                             <  1:2685616/101‑1 (MQ=255)
                cgcgCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAAcc                                                                            >  2:1810537/1‑101 (MQ=255)
                  cgcAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CCCTTCCGAGCCGGGAAACcgg                                                                          >  2:1676627/1‑100 (MQ=255)
                   gcagcaAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACcgcg                                                                         >  1:4512051/1‑101 (MQ=255)
                   gcagaaGGGCCGCGCGCAGAGCGCCAGGGTGGGCCCGGGGCAGCCAAGCGCCGCCTGCCCCGCGCCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACcgcg                                                                         <  1:440606/101‑1 (MQ=255)
                    cagcaAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCgcgc                                                                        >  2:1422052/1‑101 (MQ=255)
                     agcaAGGCCGCGCGCAGAGCGTCGGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGcc                                                                       <  2:322254/101‑1 (MQ=255)
                      gcaAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCg                                                                      >  2:3390178/1‑101 (MQ=255)
                       caAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCgg                                                                     >  1:3502658/1‑101 (MQ=255)
                         aGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGaa                                                                   <  2:3297184/101‑1 (MQ=255)
                         aGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGaa                                                                   >  2:2404095/1‑101 (MQ=255)
                         aGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCACCCAAGCGACGCCTGCGCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGaa                                                                   <  1:4239235/101‑1 (MQ=255)
                            ccGCGCGGAGAGCGTCAGGGCCGGTCACGGGCACCCAAGCGCCGCCTGCGCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCt                                                                <  2:4362748/101‑1 (MQ=255)
                            ccGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGGAACCGCGCCGGAACCt                                                                >  2:569373/1‑101 (MQ=255)
                             cgcgcgCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTc                                                               >  1:2855034/1‑101 (MQ=255)
                             cgcgcgCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTc                                                               >  2:739605/1‑101 (MQ=255)
                               cgcgcAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCgg                                                             >  1:846002/1‑101 (MQ=255)
                                gcgcAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGt                                                            <  1:4597314/101‑1 (MQ=255)
                                 cgcAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTc                                                           >  1:865384/1‑101 (MQ=255)
                                 cgcAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTc                                                           >  2:3435842/1‑101 (MQ=255)
                                 cgcAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTc                                                           >  1:4072807/1‑101 (MQ=255)
                                    agagCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCGACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAgg                                                        >  1:3945151/1‑101 (MQ=255)
                                     gagCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGNCGGAACCTCGGACAcgg                                                       >  2:1010757/1‑101 (MQ=255)
                                      agCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGt                                                      >  2:1411420/1‑101 (MQ=255)
                                       gcgggggggTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTc                                                     <  1:690110/95‑1 (MQ=255)
                                       gCGTCGGGGTCGGTCACGGGCAGCCAAGCGCCGCCTGCGCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTc                                                     <  1:1312150/101‑1 (MQ=255)
                                                tcggtcACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGcc                                            >  2:2101260/1‑101 (MQ=255)
                                                gcggtcCGGGGCGCCCAGGCGCCGCCGGCCCCGCCGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGcc                                            <  2:3570075/100‑1 (MQ=255)
                                                  ggtcACGGGCAGCCAAGCGCCGCCTGCGCCGCGGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTc                                          <  2:4511756/101‑1 (MQ=255)
                                                  ggtcACGGGCAGCCAAGCGACGCCTGCCCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTc                                          <  1:1770197/101‑1 (MQ=255)
                                                   gtcACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCg                                         >  1:1026351/1‑101 (MQ=255)
                                                   gtcACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCg                                         >  2:4200157/1‑101 (MQ=255)
                                                   gtcACGGCCAGCCAAGCGCCGCCTGCCCCGCAGCCCCCTTCCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCg                                         <  1:3780083/101‑1 (MQ=255)
                                                   gtcACGGACAGCCATGCGCCGCCTGCGCCGCACCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCg                                         <  2:2677816/101‑1 (MQ=255)
                                                   ggcACGGGCAGCCAAGCGACGCCTGCCCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCg                                         <  1:1988162/99‑1 (MQ=255)
                                                   gccgcGGGCACCCAAGCGACGCCGCCCCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCg                                         <  1:1010840/97‑1 (MQ=255)
                                                        gggCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCtt                                    >  1:3222966/1‑101 (MQ=255)
                                                        ggcccgCCAAGCGACCCCTGCCCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCtt                                    <  1:1898534/96‑1 (MQ=255)
                                                        ggccAGCCAGGCCACGCCTCGCCCACAGCCTGCTTACCCGC.CACTTCAGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCtt                                    <  2:760475/98‑1 (MQ=255)
                                                          gCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTct                                  >  1:2059752/1‑100 (MQ=255)
                                                          gCACCCAGGCGCCCCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTct                                  <  2:2472372/101‑2 (MQ=255)
                                                              ccAAGCGCCGCCTGCCCCGCACCCCCCTTCCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGAcc                              <  2:3368529/101‑1 (MQ=255)
                                                              ccAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGAcc                              >  1:2284485/1‑101 (MQ=255)
                                                               cAAGCGCCGCCTGCGCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGAccc                             <  2:4001393/101‑1 (MQ=255)
                                                               cAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGAccc                             >  2:2572339/1‑101 (MQ=255)
                                                               cAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGAccc                             >  1:2189078/1‑101 (MQ=255)
                                                               cAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGAccc                             >  2:122529/1‑101 (MQ=255)
                                                                aaGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCa                            >  1:3169622/1‑101 (MQ=255)
                                                                aaGCGACGCCGGCCCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCa                            <  2:203347/101‑1 (MQ=255)
                                                                 aGCGCCCCCTGCCCCGCACCCCCCTGGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAg                           <  2:4639585/101‑1 (MQ=255)
                                                                  gcgcccccTGCGCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGc                          <  2:1022747/95‑1 (MQ=255)
                                                                  gcgcccccTGCCCCGCACCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGc                          <  1:1422147/95‑1 (MQ=255)
                                                                   cGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCg                         >  1:2271978/1‑101 (MQ=255)
                                                                   cGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCg                         >  1:1108348/1‑101 (MQ=255)
                                                                    gcccccggcgcCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGt                        <  1:4465395/94‑1 (MQ=255)
                                                                    gACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGt                        >  1:3259203/1‑101 (MQ=255)
                                                                    cccGCCGGCCCCGCACCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGt                        <  2:853717/99‑1 (MQ=255)
                                                                     aCGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTg                       >  1:4564963/1‑101 (MQ=255)
                                                                     aCGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTg                       >  1:3817907/1‑101 (MQ=255)
                                                                      ccccTGCGCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGc                      <  2:4072521/99‑1 (MQ=255)
                                                                       gCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGcc                     >  2:1493504/1‑101 (MQ=255)
                                                                        ccTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCa                    >  1:2797297/1‑101 (MQ=255)
                                                                         cTGCCCCCCAGCCGCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGTGCAGGCCTCGGTCTTCTGACCCAGCGTGCCaa                   <  2:4148519/101‑1 (MQ=255)
                                                                          tGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAg                  >  1:3634294/1‑101 (MQ=255)
                                                                          tGCGCCGCACCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAg                  <  2:1194695/101‑1 (MQ=255)
                                                                          tGCCCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAg                  <  1:3570510/101‑1 (MQ=255)
                                                                            ccccccAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAgcg                <  2:3887454/96‑1 (MQ=255)
                                                                              ccgcAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGcc              >  2:2347086/1‑101 (MQ=255)
                                                                               cgacgCCACCCTGCCCGG.CGCTCCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCt             <  1:2976070/97‑1 (MQ=255)
                                                                                gcAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCtt            >  2:2610299/1‑101 (MQ=255)
                                                                                ccAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCtt            <  2:3077060/100‑1 (MQ=255)
                                                                                  aGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGc          <  1:3081423/101‑1 (MQ=255)
                                                                                   gCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGcc         >  1:1265357/1‑101 (MQ=255)
                                                                                    cccccTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGccc        <  1:3532167/98‑1 (MQ=255)
                                                                                     ccccTTCCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCATGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGCCCa       <  2:174906/99‑1 (MQ=255)
                                                                                     cACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGCCCa       >  1:3434065/1‑101 (MQ=255)
                                                                                     cACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGCCCa       >  1:2773373/1‑101 (MQ=255)
                                                                                     cACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTAGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGCCCa       >  2:1966724/1‑101 (MQ=255)
                                                                                      aCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGCCCAg      >  1:390096/1‑101 (MQ=255)
                                                                                       ccctcccccGCCGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGCCCAg      <  2:1949092/96‑1 (MQ=255)
                                                                                       ccTTCCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGCCCAGt     <  1:1411515/101‑1 (MQ=255)
                                                                                        cTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGCCCAGtt    >  1:3774666/1‑101 (MQ=255)
                                                                                         ttGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGCCCAGTTc   >  1:2396864/1‑101 (MQ=255)
                                                                                          tGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGCCCAGTTcc  <  1:2350839/101‑1 (MQ=255)
                                                                                          tGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGACAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGCCCAGTTcc  <  2:787570/101‑1 (MQ=255)
                                                                                               ||                                                                                               
TCGGGCGGGACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCCG.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCCGACCCAGCGTGCCAAGCGCCTTGCCCAGTTCC  >  NC_014034/3394140‑3394330

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG