Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,208,436 Δ1 bp 3.2% coding (868/948 nt) pfkB ← 6‑phosphofructokinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,208,4360C→. 3.2% 5.3 0.0 ‑0.3126coding (868/948 nt)pfkB6‑phosphofructokinase
Reads supporting (aligned to +/- strand):  new base (3/1):  ref base (75/47):  total (78/48)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.53e-01

Only 100 of 191 total aligned reads displayed.

CCTCGTCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGCCCGCG  >  NC_014034/1208336‑1208536
                                                                                                    |                                                                                                    
ccTCGTCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAc                                                                                                      <  2:1397227/101‑1 (MQ=255)
ccTCGTCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAc                                                                                                      <  1:319339/101‑1 (MQ=255)
ccTCGTCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAc                                                                                                      >  2:420632/1‑101 (MQ=255)
 cTCGTCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAcc                                                                                                     <  2:2683168/101‑1 (MQ=255)
 cTCGTCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAcc                                                                                                     >  2:1708522/1‑101 (MQ=255)
  tCGTCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccg                                                                                                    >  1:2440595/1‑101 (MQ=255)
   cGTCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgc                                                                                                   >  2:2414379/1‑101 (MQ=255)
    gTCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgcc                                                                                                  <  1:3086635/101‑1 (MQ=255)
      ggccGGTTTGGGCCTTAGATCTTTCCCAGCGCCGAGGCGCGAAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgc                                                                                                <  2:843654/99‑1 (MQ=255)
      ccccGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgc                                                                                                >  1:1684572/1‑101 (MQ=255)
      ccccGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgc                                                                                                >  1:2197563/1‑101 (MQ=255)
       cgcGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgcc                                                                                               <  2:3354913/99‑1 (MQ=255)
       cccGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgcc                                                                                               >  2:3779465/1‑101 (MQ=255)
       cccGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgcc                                                                                               >  2:4051218/1‑101 (MQ=255)
         cGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgc                                                                                             >  1:3719728/1‑101 (MQ=255)
          ggTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgcc                                                                                            <  2:3387524/101‑1 (MQ=255)
          ggTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgcc                                                                                            <  1:4363887/101‑1 (MQ=255)
          ggTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgcc                                                                                            <  2:287194/101‑1 (MQ=255)
          ggTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgcc                                                                                            <  2:2550122/101‑1 (MQ=255)
           gTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccg                                                                                           <  2:2848653/101‑1 (MQ=255)
            tttGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgc                                                                                          <  1:2158004/101‑1 (MQ=255)
             ttGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgcc                                                                                         >  2:3416599/1‑101 (MQ=255)
             ttGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgcc                                                                                         >  1:3100038/1‑101 (MQ=255)
             ttGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgcc                                                                                         <  1:3303656/101‑1 (MQ=255)
               gggTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgc                                                                                       >  2:454979/1‑101 (MQ=255)
               gggTCTCAGAACTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgc                                                                                       <  2:1895698/101‑1 (MQ=255)
                ggTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgcc                                                                                      >  2:3167506/1‑101 (MQ=255)
                 gTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgccg                                                                                     >  2:73782/1‑101 (MQ=255)
                 gTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgccg                                                                                     <  2:2987593/101‑1 (MQ=255)
                 gTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgccg                                                                                     <  1:4026894/101‑1 (MQ=255)
                   gtcAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgccgcc                                                                                   >  1:4310487/2‑101 (MQ=255)
                     cAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACcgccgccgccgccgccgccgc                                                                                 >  1:3875526/1‑101 (MQ=255)
                      aGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCcgcg                                                                                <  2:766046/101‑1 (MQ=255)
                       gATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCgcgc                                                                               >  1:2898509/1‑101 (MQ=255)
                         tCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGccc                                                                             >  1:3014948/1‑101 (MQ=255)
                         tCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGccc                                                                             <  1:2790950/101‑1 (MQ=255)
                           ttttCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCca                                                                           <  2:2565596/101‑1 (MQ=255)
                           ttttCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCACCGCCGCCGCCGCCGCGCCCca                                                                           >  2:1619748/1‑101 (MQ=255)
                           ttttCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGCGCAAAGGTCGGTGGCGGGGGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCca                                                                           >  1:2773289/1‑101 (MQ=255)
                             ttCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagc                                                                         >  1:3478298/1‑101 (MQ=255)
                             ttCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGGGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagc                                                                         >  1:618451/1‑101 (MQ=255)
                               ccAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgc                                                                       >  2:3102513/1‑101 (MQ=255)
                               ccAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgc                                                                       <  1:3416836/101‑1 (MQ=255)
                               ccAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgc                                                                       >  2:213828/1‑101 (MQ=255)
                                cAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgca                                                                      <  1:613881/101‑1 (MQ=255)
                                    gacgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCagcgcagcgc                                                                  >  1:926720/1‑101 (MQ=255)
                                    gacgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCagcgcagcgc                                                                  >  2:883131/1‑101 (MQ=255)
                                    gacgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCagcgcagcgc                                                                  >  1:224731/1‑101 (MQ=255)
                                      cgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCt                                                                <  1:1009575/101‑1 (MQ=255)
                                      cgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCt                                                                >  2:2488631/1‑101 (MQ=255)
                                       ggggCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTc                                                               <  1:1838617/99‑1 (MQ=255)
                                       gaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTc                                                               <  2:2197424/101‑1 (MQ=255)
                                        aGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCg                                                              <  1:1364381/101‑1 (MQ=255)
                                        aGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCg                                                              >  1:4109390/1‑101 (MQ=255)
                                          gcgcgcAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCggg                                                            <  1:146263/101‑1 (MQ=255)
                                           cgcgcAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGc                                                           <  1:580620/101‑1 (MQ=255)
                                             cgcAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAg                                                         <  2:2927517/101‑1 (MQ=255)
                                               cAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGc                                                       <  1:3764221/101‑1 (MQ=255)
                                               cAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGc                                                       <  2:4431150/101‑1 (MQ=255)
                                               cAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCA...CCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCa                                                    >  1:335568/1‑101 (MQ=255)
                                                  gagCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCCCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCa                                                    <  1:822928/101‑1 (MQ=255)
                                                   agCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAg                                                   <  1:2626271/101‑1 (MQ=255)
                                                   agCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAg                                                   >  2:1038995/1‑101 (MQ=255)
                                                      cGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGccc                                                <  1:3953889/101‑1 (MQ=255)
                                                      cGTTTCACATCGGCCAGCGTGCAAAGGTCGGGGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGccc                                                <  2:3051328/101‑1 (MQ=255)
                                                       gTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCcg                                               >  1:1632206/1‑101 (MQ=255)
                                                       gTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCcg                                               >  2:3355336/1‑101 (MQ=255)
                                                          tCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCgg                                            >  1:878336/1‑101 (MQ=255)
                                                           cacaTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGc                                           >  2:1160268/1‑101 (MQ=255)
                                                            acaTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGcc                                          >  2:3318243/1‑101 (MQ=255)
                                                            acaTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGcc                                          <  1:1006267/101‑1 (MQ=255)
                                                            acaTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGcc                                          <  2:1361357/101‑1 (MQ=255)
                                                            acaTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGcc                                          <  1:769717/101‑1 (MQ=255)
                                                             caTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCg                                         >  1:1228161/1‑101 (MQ=255)
                                                              aTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGa                                        >  1:2497806/1‑101 (MQ=255)
                                                                  gCCAGCGTGTAAGGGTCGGTGGTGGGTGTCATCCCCGCCGCCGCCGCCGCCGTCGCGCCCCAGCTCAGCGCCTCGGGCAGGCTCATCCCGCGGCCGAGGCt                                    <  2:1449982/101‑1 (MQ=255)
                                                                   ccAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTg                                   >  2:3934580/1‑101 (MQ=255)
                                                                    cAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTgg                                  >  1:3046312/1‑100 (MQ=255)
                                                                    cAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGa                                  >  1:498646/1‑101 (MQ=255)
                                                                    cAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGa                                  >  1:3678519/1‑101 (MQ=255)
                                                                    cAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGa                                  >  1:1093085/1‑101 (MQ=255)
                                                                      gCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAgg                                <  2:3875272/101‑1 (MQ=255)
                                                                       cGTGCAAAGGTCGGTGGCGGGTGTCATCA...CCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGa                            >  1:1553331/1‑101 (MQ=255)
                                                                         tGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTg                             >  1:194756/1‑101 (MQ=255)
                                                                         tGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCACGCGGCCGAGGCTGAGGGTg                             >  2:1106143/1‑101 (MQ=255)
                                                                          gCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGa                            >  1:4294682/1‑101 (MQ=255)
                                                                           cAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGaa                           >  1:93901/1‑101 (MQ=255)
                                                                           cAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGaa                           >  1:4434371/1‑101 (MQ=255)
                                                                            aaaGGTCGGTGGCGGGTGTCATCCCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAg                          <  2:3544423/101‑1 (MQ=255)
                                                                            aaaGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAg                          <  2:527275/101‑1 (MQ=255)
                                                                            aaaGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAg                          <  1:3320042/101‑1 (MQ=255)
                                                                             aaGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAgg                         >  1:2841433/1‑101 (MQ=255)
                                                                               ggTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAggcg                       >  2:2243109/1‑101 (MQ=255)
                                                                                gTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGCGCCTGAGGGTGAAggcgg                      >  2:401003/1‑101 (MQ=255)
                                                                                   ggTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGcga                   <  2:2688955/101‑1 (MQ=255)
                                                                                      ggCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGcgacga                >  2:1598101/1‑101 (MQ=255)
                                                                                          ggTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACt            <  2:608246/101‑1 (MQ=255)
                                                                                               catcaCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGGGAAGGCGGCGACGAAACTGTCGc       >  2:2325120/1‑101 (MQ=255)
                                                                                                atccCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGcc      <  2:4336504/101‑1 (MQ=255)
                                                                                                 tccccgccgccgccgccgccgccgCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGccc     <  2:3583528/98‑1 (MQ=255)
                                                                                                    |                                                                                                    
CCTCGTCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGCCCGCG  >  NC_014034/1208336‑1208536

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG