BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,208,437	Δ1 bp	3.2%	coding (867/948 nt)	pfkB ←	6‑phosphofructokinase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,208,437	0	C→.	3.2%	5.3 0.0 ‑0.3	125	coding (867/948 nt)	pfkB	6‑phosphofructokinase
Reads supporting (aligned to +/- strand): new base (3/1): ref base (74/47): total (77/48)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.57e-01

Only 100 of 189 total aligned reads displayed.


CTCGTCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGCCCGCGC  >  NC_014034/1208337‑1208537


cTCGTCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAcc                                                                                                      <  1:3936810/101‑1 (MQ=255)


cTCGTCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAcc                                                                                                      >  2:1708522/1‑101 (MQ=255)


cTCGTCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAcc                                                                                                      <  2:2683168/101‑1 (MQ=255)


 tCGTCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccg                                                                                                     >  1:2440595/1‑101 (MQ=255)


   gTCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgcc                                                                                                   <  1:3086635/101‑1 (MQ=255)


    tCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccg                                                                                                  >  2:3953635/1‑101 (MQ=255)


     ggccGGTTTGGGCCTTAGATCTTTCCCAGCGCCGAGGCGCGAAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgc                                                                                                 <  2:843654/99‑1 (MQ=255)


     ccccGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgc                                                                                                 <  2:1088189/101‑1 (MQ=255)


     ccccGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgc                                                                                                 >  1:1684572/1‑101 (MQ=255)


     ccccGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgc                                                                                                 >  1:2197563/1‑101 (MQ=255)


      cgcGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgcc                                                                                                <  2:3354913/99‑1 (MQ=255)


      cccGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgcc                                                                                                <  1:4185143/101‑1 (MQ=255)


      cccGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgcc                                                                                                >  2:4051218/1‑101 (MQ=255)


        cGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgc                                                                                              >  1:3719728/1‑101 (MQ=255)


         ggTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgcc                                                                                             <  2:2550122/101‑1 (MQ=255)


         ggTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgcc                                                                                             <  2:3387524/101‑1 (MQ=255)


         ggTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgcc                                                                                             <  2:287194/101‑1 (MQ=255)


          gTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccg                                                                                            <  2:2848653/101‑1 (MQ=255)


          gTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccg                                                                                            >  1:4336773/1‑101 (MQ=255)


            ttGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgcc                                                                                          >  1:3100038/1‑101 (MQ=255)


             tGGGTCTCAGATCTTTTCCAGCGAGGAGGCCCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccg                                                                                         <  1:2916604/101‑1 (MQ=255)


              gggTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgc                                                                                        >  1:608314/1‑101 (MQ=255)


              gggTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgc                                                                                        >  2:454979/1‑101 (MQ=255)


              gggTCTCAGAACTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgc                                                                                        <  2:1895698/101‑1 (MQ=255)


               ggTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgcc                                                                                       >  2:1126980/1‑101 (MQ=255)


                gTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgccg                                                                                      >  2:73782/1‑101 (MQ=255)


                gTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgccg                                                                                      <  1:4026894/101‑1 (MQ=255)


                  ctcAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgccgcc                                                                                    >  2:3472485/1‑101 (MQ=255)


                   tcAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgccgccg                                                                                   >  1:4431422/1‑101 (MQ=255)


                     aGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCcgcg                                                                                 <  2:766046/101‑1 (MQ=255)


                        tCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGccc                                                                              <  1:2790950/101‑1 (MQ=255)


                          ttttCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCca                                                                            <  2:2565596/101‑1 (MQ=255)


                          ttttCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCACCGCCGCCGCCGCCGCGCCCca                                                                            >  2:1619748/1‑101 (MQ=255)


                          ttttCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGCGCAAAGGTCGGTGGCGGGGGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCca                                                                            >  1:2773289/1‑101 (MQ=255)


                            ttCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagc                                                                          >  1:3478298/1‑101 (MQ=255)


                            ttCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGGGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagc                                                                          >  1:618451/1‑101 (MQ=255)


                             tCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcg                                                                         <  1:810741/101‑1 (MQ=255)


                             tCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcg                                                                         >  2:3933767/1‑101 (MQ=255)


                              ccAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgc                                                                        <  1:3416836/101‑1 (MQ=255)


                              ccAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgc                                                                        >  2:213828/1‑101 (MQ=255)


                                aGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgcag                                                                      <  1:3244784/101‑1 (MQ=255)


                                aGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgcag                                                                      <  1:455019/101‑1 (MQ=255)


                                   gacgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCagcgcagcgc                                                                   >  2:883131/1‑101 (MQ=255)


                                   gacgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCagcgcagcgc                                                                   >  1:224731/1‑101 (MQ=255)


                                     cgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGccc                                                                 >  2:1006182/1‑100 (MQ=255)


                                     cgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCt                                                                 <  1:1009575/101‑1 (MQ=255)


                                      ggggCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTc                                                                <  1:1838617/99‑1 (MQ=255)


                                      gaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTc                                                                <  2:2197424/101‑1 (MQ=255)


                                       aGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCg                                                               <  1:1364381/101‑1 (MQ=255)


                                         gcgcgcAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCggg                                                             <  1:146263/101‑1 (MQ=255)


                                              cAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGc                                                        <  1:3764221/101‑1 (MQ=255)


                                              cAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGc                                                        <  2:4431150/101‑1 (MQ=255)


                                              cAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCA...CCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCa                                                     >  1:335568/1‑101 (MQ=255)


                                               aaGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCt                                                       >  1:886287/1‑101 (MQ=255)


                                                  agCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAg                                                    <  1:2626271/101‑1 (MQ=255)


                                                     cGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGccc                                                 <  1:2840591/101‑1 (MQ=255)


                                                     cGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGccc                                                 >  1:4046634/1‑101 (MQ=255)


                                                      gTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCcg                                                >  1:1632206/1‑101 (MQ=255)


                                                      gTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCcg                                                >  2:3355336/1‑101 (MQ=255)


                                                       tttCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCcgc                                               >  2:244521/1‑101 (MQ=255)


                                                        ttCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCcgcg                                              >  1:3336484/1‑101 (MQ=255)


                                                          cacaTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGc                                            >  1:2872202/1‑101 (MQ=255)


                                                           acaTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGcc                                           >  2:3318243/1‑101 (MQ=255)


                                                           acaTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGcc                                           >  2:3868172/1‑101 (MQ=255)


                                                           acaTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGcc                                           <  1:1006267/101‑1 (MQ=255)


                                                           acaTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGcc                                           <  2:1361357/101‑1 (MQ=255)


                                                           acaTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGcc                                           <  2:224704/101‑1 (MQ=255)


                                                            caTCGGCCGGCGTGCAAGGGTGGGTGGCGGGTGTCACCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCg                                          <  2:3564972/101‑1 (MQ=255)


                                                            caTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCg                                          >  1:1228161/1‑101 (MQ=255)


                                                             aTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGa                                         >  1:2497806/1‑101 (MQ=255)


                                                             aTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGa                                         >  1:769373/1‑101 (MQ=255)


                                                               cGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAgg                                       >  1:2018402/1‑101 (MQ=255)


                                                                 gCCAGCGTGTAAGGGTCGGTGGTGGGTGTCATCCCCGCCGCCGCCGCCGCCGTCGCGCCCCAGCTCAGCGCCTCGGGCAGGCTCATCCCGCGGCCGAGGCt                                     <  2:1449982/101‑1 (MQ=255)


                                                                  ccAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTg                                    <  2:2891645/101‑1 (MQ=255)


                                                                   cAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGa                                   >  1:1093085/1‑101 (MQ=255)


                                                                   cAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGa                                   >  1:3678519/1‑101 (MQ=255)


                                                                      cGTGCAAAGGTCGGTGGCGGGTGTCATCA...CCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGa                             >  1:1553331/1‑101 (MQ=255)


                                                                        tGCAAAGGTCGGGGGCGGGTGTCATCCCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTg                              <  2:4326088/101‑1 (MQ=255)


                                                                         gCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCATCCCGCGGCCGAGGCTGAGGGTGa                             >  1:3316714/1‑101 (MQ=255)


                                                                           aaaGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAg                           <  2:527275/101‑1 (MQ=255)


                                                                           aaaGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAg                           >  2:3707223/1‑101 (MQ=255)


                                                                             aGGTCGGTGGGGGGTGCCATCCCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAggc                         <  2:2949357/101‑1 (MQ=255)


                                                                              ggTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAggcg                        >  2:2243109/1‑101 (MQ=255)


                                                                                 cGGGGGCGGGTGTCATCCCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGgcggcg                     <  1:4297400/101‑1 (MQ=255)


                                                                                  ggTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGcga                    <  2:2688955/101‑1 (MQ=255)


                                                                                   gTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGcgac                   <  2:3099814/101‑1 (MQ=255)


                                                                                    tGGCGGGTGTCATCA...CCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGGGAAGGCGGCGACGaaa               >  1:1874565/1‑101 (MQ=255)


                                                                                     ggCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGcgacga                 >  2:1598101/1‑101 (MQ=255)


                                                                                     ggCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGcgacga                 <  2:1228068/101‑1 (MQ=255)


                                                                                       cGGGTGTCATCCCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGCCCGAGGCTGAGGGTGAAGGCGGCGACGaaa               <  1:3966710/101‑1 (MQ=255)


                                                                                        gggTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGGCGAAAc              >  1:829138/1‑101 (MQ=255)


                                                                                        gggTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAAc              >  1:50164/1‑101 (MQ=255)


                                                                                         ggTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACt             <  2:608246/101‑1 (MQ=255)


                                                                                         ggTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACt             <  2:3404431/101‑1 (MQ=255)


                                                                                           tgtCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGt           >  2:302257/1‑101 (MQ=255)


                                                                                           tgtCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGt           >  2:2328281/1‑101 (MQ=255)


                                                                                            gtCATCCCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTc          <  2:3122368/101‑1 (MQ=255)


                                                                                               atccCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGcc       <  2:4336504/101‑1 (MQ=255)


                                                                                                 ccccgccgccgccgccgccgccgCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGCCcg     <  1:937362/99‑1 (MQ=255)


                                                                                                   ccgccgccgccgccgccgccgCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGCCcgcg   <  1:3041425/101‑1 (MQ=255)


CTCGTCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGCCCGCGC  >  NC_014034/1208337‑1208537

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG