BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,208,438	Δ1 bp	3.2%	coding (866/948 nt)	pfkB ←	6‑phosphofructokinase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,208,438	0	G→.	3.2%	5.4 0.0 ‑0.5	125	coding (866/948 nt)	pfkB	6‑phosphofructokinase
Reads supporting (aligned to +/- strand): new base (3/1): ref base (74/47): total (77/48)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.51e-01

Only 100 of 186 total aligned reads displayed.


TCGTCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGCCCGCGCC  >  NC_014034/1208338‑1208538


tCGTCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccg                                                                                                      >  1:2440595/1‑101 (MQ=255)


 cGTCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgc                                                                                                     >  2:2414379/1‑101 (MQ=255)


  gTCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgcc                                                                                                    >  1:2446871/1‑101 (MQ=255)


    ggccGGTTTGGGCCTTAGATCTTTCCCAGCGCCGAGGCGCGAAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgc                                                                                                  <  2:843654/99‑1 (MQ=255)


    ccccGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgc                                                                                                  <  2:1088189/101‑1 (MQ=255)


    ccccGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgc                                                                                                  >  1:2197563/1‑101 (MQ=255)


     cccGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgcc                                                                                                 >  2:3779465/1‑101 (MQ=255)


       cGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgc                                                                                               >  1:3164101/1‑101 (MQ=255)


       cGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgc                                                                                               >  1:3719728/1‑101 (MQ=255)


        ggTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgcc                                                                                              <  1:4363887/101‑1 (MQ=255)


         gTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccg                                                                                             <  2:2848653/101‑1 (MQ=255)


         gTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccg                                                                                             >  2:3331602/1‑101 (MQ=255)


           ttGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgcc                                                                                           <  1:3303656/101‑1 (MQ=255)


            tGGGTCTCAGATCTTTTCCAGCGAGGAGGCCCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccg                                                                                          <  1:2916604/101‑1 (MQ=255)


            tGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccg                                                                                          <  2:1878650/101‑1 (MQ=255)


             gggTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgc                                                                                         >  1:608314/1‑101 (MQ=255)


              ggTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgcc                                                                                        >  2:1126980/1‑101 (MQ=255)


               gTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgccg                                                                                       <  1:1812423/101‑1 (MQ=255)


               gTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgccg                                                                                       <  1:4026894/101‑1 (MQ=255)


                 gtcAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgccgcc                                                                                     >  1:4310487/2‑101 (MQ=255)


                 ctcAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgccgcc                                                                                     >  2:3472485/1‑101 (MQ=255)


                  tcAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgccgccg                                                                                    >  2:1364284/1‑101 (MQ=255)


                  tcAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCAccgccgccgccgccgccgccg                                                                                    >  2:3358131/1‑101 (MQ=255)


                       tCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGccc                                                                               >  1:3014948/1‑101 (MQ=255)


                         ttttCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCACCGCCGCCGCCGCCGCGCCCca                                                                             >  2:1619748/1‑101 (MQ=255)


                           ttCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGGGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagc                                                                           >  1:618451/1‑101 (MQ=255)


                            tCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcg                                                                          >  1:3404668/1‑101 (MQ=255)


                              cAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgca                                                                        <  2:2229841/101‑1 (MQ=255)


                               aGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgcag                                                                       <  1:455019/101‑1 (MQ=255)


                                gCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCcagcgcagc                                                                      >  2:2171479/1‑101 (MQ=255)


                                 cgacgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGGGGCGGGTGTCATCACCGCCGCCGCCGCCGGCGCCGCGCCCcagcgcagcg                                                                     >  1:2520655/1‑101 (MQ=255)


                                  gacgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCagcgcagcgc                                                                    >  1:926720/1‑101 (MQ=255)


                                  gacgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCagcgcagcgc                                                                    >  2:883131/1‑101 (MQ=255)


                                  gacgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCagcgcagcgc                                                                    >  1:224731/1‑101 (MQ=255)


                                    cgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGccc                                                                  >  2:1006182/1‑100 (MQ=255)


                                    cgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCt                                                                  >  2:2488631/1‑101 (MQ=255)


                                    cgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCt                                                                  <  1:1009575/101‑1 (MQ=255)


                                    cgaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCt                                                                  >  2:1505748/1‑101 (MQ=255)


                                     gaGGCGCGCAAGAGCCGTTTCACATCGGCTAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTc                                                                 <  2:741807/101‑1 (MQ=255)


                                     gaGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTc                                                                 >  2:580552/1‑101 (MQ=255)


                                      aGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCg                                                                >  1:4109390/1‑101 (MQ=255)


                                      aGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCg                                                                <  1:1364381/101‑1 (MQ=255)


                                        gcgcgcAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCggg                                                              <  1:146263/101‑1 (MQ=255)


                                         cgcgcAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGc                                                             <  1:2693621/101‑1 (MQ=255)


                                             cAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGc                                                         <  2:4431150/101‑1 (MQ=255)


                                             cAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGc                                                         <  1:2788473/101‑1 (MQ=255)


                                             cAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGc                                                         <  1:3764221/101‑1 (MQ=255)


                                             cAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCA...CCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCa                                                      >  1:335568/1‑101 (MQ=255)


                                                gagCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCCCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCa                                                      <  1:822928/101‑1 (MQ=255)


                                                 agCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAg                                                     >  1:3088501/1‑101 (MQ=255)


                                                    cGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGccc                                                  <  1:3953889/101‑1 (MQ=255)


                                                    cGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGccc                                                  <  1:2840591/101‑1 (MQ=255)


                                                     gTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCcg                                                 >  2:4087650/1‑101 (MQ=255)


                                                     gTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCcg                                                 >  1:1632206/1‑101 (MQ=255)


                                                       ttCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCcgcg                                               >  2:509844/1‑101 (MQ=255)


                                                       ttCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCcgcg                                               <  2:2634349/101‑1 (MQ=255)


                                                        tCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCgg                                              >  1:878336/1‑101 (MQ=255)


                                                        tCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCgg                                              >  1:3455131/1‑101 (MQ=255)


                                                         cacaTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGc                                             >  1:2872202/1‑101 (MQ=255)


                                                          acaTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGcc                                            <  2:224704/101‑1 (MQ=255)


                                                          acaTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGcc                                            >  2:3318243/1‑101 (MQ=255)


                                                          acaTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGcc                                            <  2:618383/101‑1 (MQ=255)


                                                          acaTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGcc                                            <  1:1006267/101‑1 (MQ=255)


                                                          acaTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGcc                                            <  1:769717/101‑1 (MQ=255)


                                                           caTCGGCCGGCGTGCAAGGGTGGGTGGCGGGTGTCACCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCg                                           <  2:3564972/101‑1 (MQ=255)


                                                           caTCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCg                                           >  1:1228161/1‑101 (MQ=255)


                                                              cGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAgg                                        >  1:2018402/1‑101 (MQ=255)


                                                                 ccAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTg                                     >  2:3934580/1‑101 (MQ=255)


                                                                 ccAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTg                                     <  2:2891645/101‑1 (MQ=255)


                                                                  cAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTgg                                    >  1:3046312/1‑100 (MQ=255)


                                                                  cAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGa                                    >  1:1093085/1‑101 (MQ=255)


                                                                   aGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAg                                   >  2:4437212/1‑101 (MQ=255)


                                                                    gCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAgg                                  <  2:3875272/101‑1 (MQ=255)


                                                                     cGTGCAAAGGTCGGTGGCGGGTGTCATCA...CCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGa                              >  1:1553331/1‑101 (MQ=255)


                                                                      gTGCAAAGGTCGGTGGCGGGTGTCATCA...CCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGaa                             <  2:335533/101‑1 (MQ=255)


                                                                       tGCAACGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTg                               >  2:212549/1‑101 (MQ=255)


                                                                        gCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGa                              >  1:4294682/1‑101 (MQ=255)


                                                                         cAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGaa                             >  1:4434371/1‑101 (MQ=255)


                                                                          aaaGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAg                            <  1:3320042/101‑1 (MQ=255)


                                                                          aaaGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAg                            >  2:3707223/1‑101 (MQ=255)


                                                                            aGGTCGGTGGGGGGTGCCATCCCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAggc                          <  2:2949357/101‑1 (MQ=255)


                                                                             ggTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAggcg                         >  2:28492/1‑101 (MQ=255)


                                                                              gTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGCGCCTGAGGGTGAAggcgg                        >  2:401003/1‑101 (MQ=255)


                                                                                 ggTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGcga                     <  2:2688955/101‑1 (MQ=255)


                                                                                  gTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGcgac                    <  2:3099814/101‑1 (MQ=255)


                                                                                   tGGCGGGTGTCATCA...CCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGGGAAGGCGGCGACGaaa                >  1:1874565/1‑101 (MQ=255)


                                                                                    ggCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGcgacga                  >  2:1598101/1‑101 (MQ=255)


                                                                                    ggCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGcgacga                  <  2:1228068/101‑1 (MQ=255)


                                                                                      cGGGTGTCATCCCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGCCCGAGGCTGAGGGTGAAGGCGGCGACGaaa                <  1:3966710/101‑1 (MQ=255)


                                                                                       gggTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAAc               >  1:50164/1‑101 (MQ=255)


                                                                                        ggTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACt              <  2:3404431/101‑1 (MQ=255)


                                                                                          tgttgcccccgcccccgccgctgccgccgCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGt            <  1:363991/93‑1 (MQ=255)


                                                                                          tgtCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGt            >  2:302257/1‑101 (MQ=255)


                                                                                           gtCATCCCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTc           <  2:3122368/101‑1 (MQ=255)


                                                                                             catcaCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGGGAAGGCGGCGACGAAACTGTCGc         >  2:2325120/1‑101 (MQ=255)


                                                                                              atccCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGcc        <  2:4336504/101‑1 (MQ=255)


                                                                                                ccccgccgccgccgccgccgccgCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGCCcg      <  1:937362/99‑1 (MQ=255)


                                                                                                ccccgccgccgccgccgccgccgCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGCCcg      <  1:1668968/99‑1 (MQ=255)


                                                                                                caCCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGCCcg      >  2:2551241/1‑101 (MQ=255)


                                                                                                    gccgccgccgccgccgccgcGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGCCCGCGcc  <  1:3702603/101‑1 (MQ=255)


TCGTCCCCGGTTTGGGTCTCAGATCTTTTCCAGCGACGAGGCGCGCAAGAGCCGTTTCACATCGGCCAGCGTGCAAAGGTCGGTGGCGGGTGTCATCACCGCCGCCGCCGCCGCCGCCGCGCCCCAGCGCAGCGCCTCGGGCAGGCTCAGCCCGCGGCCGAGGCTGAGGGTGAAGGCGGCGACGAAACTGTCGCCCGCGCC  >  NC_014034/1208338‑1208538

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG