Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,253,263 Δ1 bp 2.4% coding (241/2421 nt) copA2 ← copper‑transporting P‑type ATPase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,253,2630C→. 2.4% 2.6 ‑3.4 0.0165coding (241/2421 nt)copA2copper‑transporting P‑type ATPase
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (93/68):  total (93/72)
Fisher's exact test strand distribution p-value = 3.46e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 191 total aligned reads displayed.

ACCGTGCCCTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCGGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTCCGCCCGCGCCGG  >  NC_014034/1253163‑1253359
                                                                                                     |                                                                                                
   gTGCCCTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCgg                                                                                               <  1:2917247/101‑1 (MQ=255)
   gTGCCCTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCgg                                                                                               >  1:4186657/1‑101 (MQ=255)
    tGCCCTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCggg                                                                                              <  1:1277706/101‑1 (MQ=255)
     gCCCTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGCCACGACCCTGTCCGCCgggg                                                                                             >  2:1313967/1‑101 (MQ=255)
     gCCCTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCgggg                                                                                             >  1:542261/1‑101 (MQ=255)
     gCCCTGCGCATAGCTGACGCGGGCGCTGCCCTCGCGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCgggg                                                                                             <  1:3828153/101‑1 (MQ=255)
      cccTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCggggg                                                                                            <  1:2783282/101‑1 (MQ=255)
       ccTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCgggggg                                                                                           >  1:206282/1‑101 (MQ=255)
        cTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCggggggg                                                                                          <  2:1441619/101‑1 (MQ=255)
        cTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCggggggg                                                                                          >  1:1572781/1‑101 (MQ=255)
         tGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCgggggggg                                                                                         <  1:759108/101‑1 (MQ=255)
          gcgcGTGGGTGCCGCGGGCGCTGCTGTCGGGAAAAAGCAGGACAGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGGCACGCCCCTGTCCGCCggggatggg                                                                                        <  2:115898/101‑6 (MQ=255)
          gcgcATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCggggggggg                                                                                        >  2:2912873/1‑101 (MQ=255)
             cATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCGGGGGGGGGGCg                                                                                     >  2:4409078/1‑101 (MQ=255)
              aTAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCgggggggggcgtc                                                                                    <  2:6477/101‑5 (MQ=255)
              aTAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCgggggggggcggc                                                                                    >  2:4207736/1‑97 (MQ=255)
                aGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGa                                                                                 >  2:205352/1‑101 (MQ=255)
                 ggTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGaa                                                                                <  2:3108082/101‑1 (MQ=255)
                    gACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGcc                                                                             >  2:2453907/1‑101 (MQ=255)
                     aCGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGccc                                                                            <  2:2096849/101‑1 (MQ=255)
                      cgcgGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCCCCCC.GGGCGTCGCCTGCAGCGCGGACACGCCCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCa                                                                           <  1:375068/101‑1 (MQ=255)
                       gggggCGCGGCCCTCGGGAAAAGGCAGG.CCGGGGGCCCCCCGGGGCGTCGCCGCCAGCCCGGACCCGCCCCTGCCCG.CGGGGGGGGGGCGTCGAAGCCCAg                                                                          <  1:4126703/99‑1 (MQ=255)
                       gcgGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCCCCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAg                                                                          <  2:393196/101‑1 (MQ=255)
                       gcgGGCCCTGCCCTCGGGCAAAAGCAGGACCGAGAGCCCCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAg                                                                          <  1:395256/101‑1 (MQ=255)
                         gggCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGAc                                                                        <  1:1682362/101‑1 (MQ=255)
                         gggCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC..GGGGGGGGCGTCGAAGCCCAGACa                                                                       >  1:3690025/1‑101 (MQ=255)
                          ggCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACa                                                                       <  2:2489828/101‑1 (MQ=255)
                             gcTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCGGGGGGGGGGCGTCGAAGCCCAGCCAgg                                                                     >  1:2022626/1‑101 (MQ=255)
                             gcTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAggc                                                                    >  2:1855237/1‑100 (MQ=255)
                             gcTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGt                                                                    >  2:3387487/1‑101 (MQ=255)
                                gCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGtctc                                                                 <  1:190455/101‑1 (MQ=255)
                                 cccTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTctcg                                                                >  1:1008119/1‑101 (MQ=255)
                                 cccTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTctcg                                                                <  1:1038658/101‑1 (MQ=255)
                                  ccTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCGGGGGGGGGG.GTCGAAGTCCAGACAGGTctcgg                                                               >  2:3898293/1‑101 (MQ=255)
                                    tCGGGCACAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGGAGCGCGGACACGCCCCTGTCCGCCGGGGGGGGGG.GTCGAGGCCCAGACAGGTctgggcc                                                             >  2:2068309/1‑101 (MQ=255)
                                    tCGGGCAAAAGCAGGACCGAGAGCCCCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTctcggcc                                                             <  1:713677/101‑1 (MQ=255)
                                     cGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTctcggcct                                                            >  2:1886897/1‑101 (MQ=255)
                                     cGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTctcggcct                                                            <  2:4300214/101‑1 (MQ=255)
                                     cGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGGctcggcct                                                            >  1:2157570/1‑101 (MQ=255)
                                       ggCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTctcggcctcg                                                          <  2:3605740/101‑1 (MQ=255)
                                        gCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTctcggcctcgg                                                         >  2:3785237/1‑101 (MQ=255)
                                         cAAAAGCAGGACCGAGAGCCCCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTctcggcctcggc                                                        <  2:2664750/101‑1 (MQ=255)
                                         cAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTctcggcctcggc                                                        <  1:2093503/101‑1 (MQ=255)
                                          aaaaGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCGGGGGGGGGG.GGCGAAGCCCAAAAAGGGCCCGGCCCCGGCa                                                       >  1:3024239/1‑101 (MQ=255)
                                           aaaGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGGAACCCCGACAGGGctccgcccccggca                                                      >  1:877654/1‑94 (MQ=255)
                                            aggCAGGCCCGAGGGCCCCCG.GGGCGTCGCCTGCGGCGCGGACCCGCCCCGGCCCG.CGGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAAt                                                     <  1:2506697/99‑1 (MQ=255)
                                               cAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGCCTCGGCCTCGGCAATCgg                                                  >  2:1126329/1‑101 (MQ=255)
                                                aGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGa                                                 >  1:4073988/1‑101 (MQ=255)
                                                aGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGa                                                 >  1:513434/1‑101 (MQ=255)
                                                aGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGa                                                 >  1:3621463/1‑101 (MQ=255)
                                                   aCCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAg                                              <  2:280604/101‑1 (MQ=255)
                                                   aCCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC..GGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgc                                             <  2:2989447/101‑1 (MQ=255)
                                                    ccGAGAGCCCCCC.GGGCGTCGCCTGCAGCGCCGACCCGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgc                                             <  1:3284709/101‑1 (MQ=255)
                                                    ccGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgc                                             <  2:841678/101‑1 (MQ=255)
                                                      gagaGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgccc                                           <  2:2121895/101‑1 (MQ=255)
                                                      gagaGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgccc                                           <  1:1456051/101‑1 (MQ=255)
                                                      gagaGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgccc                                           <  1:3343763/101‑1 (MQ=255)
                                                       agagCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgcccg                                          >  2:921240/1‑101 (MQ=255)
                                                        gagCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgcccgc                                         >  1:999213/1‑101 (MQ=255)
                                                          gcccccc.GGGCGCCGCCTGCAGCGCGGACACGACCCTGTCCG.CGGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgcccgccc                                       <  1:2408629/98‑1 (MQ=255)
                                                           cccccc.GGGCGTCGCCTGCAGCGCGGACACGACCCTGCCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGcccgcccg                                      <  1:2613899/99‑1 (MQ=255)
                                                            aCCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACGGGTCTCGGCCTCGGCAATCGGATAGCccgcccgc                                     >  1:3807959/1‑101 (MQ=255)
                                                            aCCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCccgcccgc                                     >  1:651879/1‑101 (MQ=255)
                                                               cc.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGcc                                  <  2:2609502/101‑1 (MQ=255)
                                                                  gggCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAg                                <  1:2563281/101‑1 (MQ=255)
                                                                   ggCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAgc                               >  2:4236504/1‑101 (MQ=255)
                                                                    gggTCGCCTGCAGCGCGGACCCGCCCCTGTCCGCGGGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAgc                               <  2:2022489/99‑1 (MQ=255)
                                                                    gCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCGGGGGGGGGG.GTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAgcg                              >  1:2006419/1‑101 (MQ=255)
                                                                      ggcGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGcc                            <  2:1644980/99‑1 (MQ=255)
                                                                      gTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGcc                            >  1:3082452/1‑101 (MQ=255)
                                                                      gTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGcc                            >  2:1376631/1‑101 (MQ=255)
                                                                        cGCCTGCAGCGCGGACACGCCCCTGTCCG.CGGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTc                          <  1:3927213/101‑1 (MQ=255)
                                                                        cGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTc                          <  2:691378/101‑1 (MQ=255)
                                                                        cGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTc                          >  1:1642923/1‑101 (MQ=255)
                                                                        cGCCTGCAGCGCGGACACGACCCTGTCCG.CGGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTc                          <  2:2157431/101‑1 (MQ=255)
                                                                         gCCTGCAGCGGGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCg                         <  1:2742605/101‑1 (MQ=255)
                                                                          ccggCAGCGCGGACACGCCCCTGTCC.CCGGGGGGGGGGCGTCGAAGCCCAGACATGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCgg                        <  2:934781/98‑1 (MQ=255)
                                                                          ccTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCgg                        <  2:3455836/101‑1 (MQ=255)
                                                                          ccTGCAGCGCGGACACGACCCTGTCC.CCCGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCgg                        <  1:2848058/101‑1 (MQ=255)
                                                                           cTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGc                       <  2:874995/101‑1 (MQ=255)
                                                                           cTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGc                       >  1:3167653/1‑101 (MQ=255)
                                                                            tGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGcc                      <  1:3218911/101‑1 (MQ=255)
                                                                            tGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGcc                      <  1:160649/101‑1 (MQ=255)
                                                                            tGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGcc                      >  2:660054/1‑101 (MQ=255)
                                                                             gCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCa                     <  2:1008034/101‑1 (MQ=255)
                                                                             gCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCa                     >  1:679001/1‑101 (MQ=255)
                                                                              cAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAg                    >  1:1402336/1‑101 (MQ=255)
                                                                               aGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCCgg                   >  2:341478/1‑101 (MQ=255)
                                                                                 cgcgGACACGCCCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGt                 <  2:600809/101‑1 (MQ=255)
                                                                                   cgGACACGCCCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGttt               <  1:626300/101‑1 (MQ=255)
                                                                                    ggACACGGCCCTGTCC.CCGGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTc              <  2:2331108/101‑1 (MQ=255)
                                                                                    ggACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTc              >  1:864851/1‑101 (MQ=255)
                                                                                     gACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCCCCCGCGCCAGCGCCTCGGCCAGGGTTTcc             >  1:2209218/1‑101 (MQ=255)
                                                                                      acacGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCCGCGCCTCGGCCAGGGTTTccc            >  2:1332903/1‑100 (MQ=255)
                                                                                      acacGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCCCCCGCGCCAGCGCCTCGGCCAGGGGTTccg            >  1:1865723/1‑101 (MQ=255)
                                                                                       cacGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTccgc           <  1:2031102/101‑1 (MQ=255)
                                                                                        acGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTccgcc          <  1:548425/101‑1 (MQ=255)
                                                                                          gACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTccgcccg        >  2:2253045/1‑101 (MQ=255)
                                                                                          gACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTccgcccg        <  1:2220408/101‑1 (MQ=255)
                                                                                                gTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTCCGCCCGCGCCgg  >  2:196961/1‑101 (MQ=255)
                                                                                                     |                                                                                                
ACCGTGCCCTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCC.GGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCGGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTCCGCCCGCGCCGG  >  NC_014034/1253163‑1253359

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG