Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,253,265 Δ1 bp 100% coding (239/2421 nt) copA2 ← copper‑transporting P‑type ATPase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,253,2650G→.100.0% 23.4 137coding (239/2421 nt)copA2copper‑transporting P‑type ATPase
Reads supporting (aligned to +/- strand):  new base (67/60):  ref base (4/5):  total (71/66)
Fisher's exact test strand distribution p-value = 7.37e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.58e-03

Only 100 of 191 total aligned reads displayed.

CGTGCCCTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCGGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTCCGCCCGCGCCGGGG  >  NC_014034/1253165‑1253361
                                                                                                    |                                                                                                
 gTGCCCTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCgg                                                                                                 <  1:2917247/101‑1 (MQ=255)
  tGCCCTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCggg                                                                                                <  1:1277706/101‑1 (MQ=255)
   gCCCTGCGCATAGCTGACGCGGGCGCTGCCCTCGCGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCgggg                                                                                               <  1:3828153/101‑1 (MQ=255)
    cccTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCggggg                                                                                              <  1:2783282/101‑1 (MQ=255)
     ccTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCgggggg                                                                                             >  1:206282/1‑101 (MQ=255)
      cTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCggggggg                                                                                            >  1:1572781/1‑101 (MQ=255)
      cTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCggggggg                                                                                            <  2:3640185/101‑1 (MQ=255)
       tGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCgggggggg                                                                                           >  2:4335032/1‑101 (MQ=255)
        gcgcGTGGGTGCCGCGGGCGCTGCTGTCGGGAAAAAGCAGGACAGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGGCACGCCCCTGTCCGCCggggatggg                                                                                          <  2:115898/101‑6 (MQ=255)
        gcgcATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCggggggggg                                                                                          >  2:2912873/1‑101 (MQ=255)
          gcATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCggggggggggg                                                                                        >  2:2780263/1‑100 (MQ=255)
           cATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCgggggggggcgt                                                                                       >  2:2170598/1‑98 (MQ=255)
           cATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCgggggggggcgt                                                                                       >  2:2030578/1‑98 (MQ=255)
           cATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCgggggggggcgt                                                                                       >  1:600877/1‑98 (MQ=255)
            aTAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCgggggggggcgtc                                                                                      <  2:6477/101‑5 (MQ=255)
            aTAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCgggggggggcgtc                                                                                      <  2:1906685/101‑5 (MQ=255)
             tAGGTGACGCGGGCGCGGCCCTCGGGCAAAAGCAGGACCGGGAGCCCCCCGGGCGTCCCCGGCGGCGCGGACACGCCCCTGCCCG.CGGGGGGGGGGCGTCg                                                                                    <  2:1386549/101‑1 (MQ=255)
               ggTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGaa                                                                                  >  2:2490706/1‑101 (MQ=255)
                    cgcgGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCCCCCCGGGCGTCGCCTGCAGCGCGGACACGCCCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCa                                                                             <  1:375068/101‑1 (MQ=255)
                     gcgGGCCCTGCCCTCGGGCAAAAGCAGGACCGAGAGCCCCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAg                                                                            <  1:395256/101‑1 (MQ=255)
                       gggCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGAc                                                                          <  1:1682362/101‑1 (MQ=255)
                       gggCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC..GGGGGGGGCGTCGAAGCCCAGACa                                                                         >  1:3690025/1‑101 (MQ=255)
                          cgcTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGACTCCAGCGCGGACACGCCCCTGTGCGCC.GGGGGGGGGCGTCGAAGCCCAGACAgg                                                                       >  2:3562993/1‑101 (MQ=255)
                           gcTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCGGGGGGGGGGCGTCGAAGCCCAGCCAgg                                                                       >  1:2022626/1‑101 (MQ=255)
                             tGCCCTCGGGGAAAAGGAGGACCGAGAGAACCCCGGCCGTCGCCTGCAGCGCGGACACGCCACTGCCCGCCGGGGGGGGGG.GGCGAAGCACAGACAGGact                                                                    >  2:1328231/1‑101 (MQ=255)
                              gCCCTTGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGtctc                                                                   >  2:3926959/1‑101 (MQ=255)
                              gCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGtctc                                                                   <  1:190455/101‑1 (MQ=255)
                               cccTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTctcg                                                                  >  1:1008119/1‑101 (MQ=255)
                               cccTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTctcg                                                                  <  1:1038658/101‑1 (MQ=255)
                                  tCGGGCACAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGGAGCGCGGACACGCCCCTGTCCGCCGGGGGGGGGG.GTCGAGGCCCAGACAGGTctgggcc                                                               >  2:2068309/1‑101 (MQ=255)
                                   cGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGGctcggcct                                                              >  1:2157570/1‑101 (MQ=255)
                                       cAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTctcggcctcggc                                                          <  1:2093503/101‑1 (MQ=255)
                                        aaaaGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCGGGGGGGGGG.GGCGAAGCCCAAAAAGGGCCCGGCCCCGGCa                                                         >  1:3024239/1‑101 (MQ=255)
                                         aaaGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCaa                                                        >  2:2480548/1‑101 (MQ=255)
                                          aggCAGGCCCGAGGGCCCCCGGGGCGTCGCCTGCGGCGCGGACCCGCCCCGGCCCG.CGGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAAt                                                       <  1:2506697/99‑1 (MQ=255)
                                          aaGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAAt                                                       >  2:1207225/1‑101 (MQ=255)
                                           acgaGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATc                                                      <  2:2954866/98‑1 (MQ=255)
                                           accAGGCCCGAGAGCGCCCGGGGGGTCGCCTGCAGCGCGGCCCCGCCCCTGTCCG.CGGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATc                                                      <  1:712969/99‑1 (MQ=255)
                                             cAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCgg                                                    >  2:2110932/1‑101 (MQ=255)
                                             cAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCgg                                                    >  1:531408/1‑101 (MQ=255)
                                              aGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGa                                                   <  2:2990591/101‑1 (MQ=255)
                                              aGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGa                                                   >  1:4073988/1‑101 (MQ=255)
                                              aGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGa                                                   >  1:3621463/1‑101 (MQ=255)
                                                gacccggagCACCCCGGGCGGCGCCTGCAGCGCGGAAACTACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATa                                                 <  2:2622277/95‑1 (MQ=255)
                                                 aCCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAg                                                >  2:1105451/1‑101 (MQ=255)
                                                  ccGAGAGCCCCCCGGGCGTCGCCTGCAGCGCCGACCCGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgc                                               <  1:3284709/101‑1 (MQ=255)
                                                  ccGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgc                                               <  2:841678/101‑1 (MQ=255)
                                                    gagaGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgccc                                             >  2:167814/1‑101 (MQ=255)
                                                    gagaGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgccc                                             <  1:1456051/101‑1 (MQ=255)
                                                    gagaGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgccc                                             <  1:3343763/101‑1 (MQ=255)
                                                     agagCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgcccg                                            >  2:921240/1‑101 (MQ=255)
                                                      gagCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATGgcccgc                                           >  2:3387466/1‑101 (MQ=255)
                                                      gagCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgcccgc                                           <  1:651558/101‑1 (MQ=255)
                                                      gagCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgcccgc                                           >  2:1883786/1‑101 (MQ=255)
                                                        gccccccGGGCGCCGCCTGCAGCGCGGACACGACCCTGTCCG.CGGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgcccgccc                                         <  1:2408629/98‑1 (MQ=255)
                                                         ccccccGGGCGTCGCCTGCAGCGCGGACACGACCCTGCCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGcccgcccg                                        <  1:2613899/99‑1 (MQ=255)
                                                          aCCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACGGGTCTCGGCCTCGGCAATCGGATAGCccgcccgc                                       >  1:3807959/1‑101 (MQ=255)
                                                          aCCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCccgcccgc                                       >  2:3961251/1‑101 (MQ=255)
                                                           ccccGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCcgcg                                      >  2:1515466/1‑101 (MQ=255)
                                                              cGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCGGGGGGGGGG.GTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGccc                                   >  1:652364/1‑100 (MQ=255)
                                                              cGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCa                                   <  1:4177477/101‑1 (MQ=255)
                                                              cGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCa                                   >  2:3411084/1‑101 (MQ=255)
                                                               gggCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAg                                  <  1:2563281/101‑1 (MQ=255)
                                                                ggCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAgc                                 >  2:4236504/1‑101 (MQ=255)
                                                                ggCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAgc                                 >  2:345524/1‑101 (MQ=255)
                                                                 gCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCGGGGGGGGGG.GTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAgcg                                >  1:2006419/1‑101 (MQ=255)
                                                                   gtgcccTGAAGCGCGGGCCCGCCCCTGTCCGCCGGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAgcgc                               <  1:4409350/97‑1 (MQ=255)
                                                                   gTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGcc                              >  1:3082452/1‑101 (MQ=255)
                                                                     cGCCTGCAGCGCGGACACGCCCCTGTCCG.CGGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTc                            <  1:3927213/101‑1 (MQ=255)
                                                                     cGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTc                            >  1:1642923/1‑101 (MQ=255)
                                                                     cGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTc                            <  2:691378/101‑1 (MQ=255)
                                                                      gCCTGCAGCGGGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCg                           <  1:2742605/101‑1 (MQ=255)
                                                                      gCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCCGCGCCTCg                           >  2:2427314/1‑101 (MQ=255)
                                                                      gCCGGCAGCGCGGACACGCCCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCg                           <  2:2321419/101‑1 (MQ=255)
                                                                       ccggCAGCGCGGACACGCCCCTGTCC.CCGGGGGGGGGGCGTCGAAGCCCAGACATGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCgg                          <  2:934781/98‑1 (MQ=255)
                                                                       ccTGCAGCGCGGACACGACCCTGTCC.CCCGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCgg                          <  1:2848058/101‑1 (MQ=255)
                                                                        cTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGc                         <  2:874995/101‑1 (MQ=255)
                                                                        cTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGc                         >  1:3167653/1‑101 (MQ=255)
                                                                        cTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGc                         >  2:2122194/1‑101 (MQ=255)
                                                                        cTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAACCCGCCCCCGCCAGCGCCTCGGc                         >  1:786769/1‑101 (MQ=255)
                                                                         tGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGcc                        <  1:160649/101‑1 (MQ=255)
                                                                         tGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGcc                        >  2:660054/1‑101 (MQ=255)
                                                                         tGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGcc                        <  1:3218911/101‑1 (MQ=255)
                                                                           cAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAg                      >  1:1402336/1‑101 (MQ=255)
                                                                              cgcgGACACGCCCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGt                   <  2:600809/101‑1 (MQ=255)
                                                                              cgcgGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGt                   >  2:3860239/1‑101 (MQ=255)
                                                                              cgcgGACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGt                   >  2:1369051/1‑101 (MQ=255)
                                                                                 ggACACGCCCCTGTCCG.CGGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTc                <  1:865411/101‑1 (MQ=255)
                                                                                  gACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCCCCCGCGCCAGCGCCTCGGCCAGGGTTTcc               >  1:2209218/1‑101 (MQ=255)
                                                                                  gACACGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCCAGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCACCCACGGTTTcc               >  1:754391/1‑101 (MQ=255)
                                                                                   acacGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTccg              >  1:978429/1‑101 (MQ=255)
                                                                                   acacGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCCCCCGCGCCAGCGCCTCGGCCAGGGGTTccg              >  1:1865723/1‑101 (MQ=255)
                                                                                    cacGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTccgc             <  1:2031102/101‑1 (MQ=255)
                                                                                    cacGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTccgc             >  2:1005880/1‑101 (MQ=255)
                                                                                     acGACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTccgcc            <  1:548425/101‑1 (MQ=255)
                                                                                      cGACCCTGTCCG.CGGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTccgccc           <  2:2017324/101‑1 (MQ=255)
                                                                                       gACCCTGTCCGCC.GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTccgcccg          <  1:2220408/101‑1 (MQ=255)
                                                                                          ccTGTCCG.CGGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTCCGCCCgcgc       <  2:4298360/101‑1 (MQ=255)
                                                                                            tGTCCG.CGGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTCCGCCCGCGCCg     <  2:2091848/101‑1 (MQ=255)
                                                                                                ccgcgGGGGGAGTGCGTCGAAGCCCAGCCAGGTCGCGGCCTCGGCAATCGGATTTCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTCCGCCCGCGCCgggg  <  2:513382/98‑1 (MQ=38)
                                                                                                    |                                                                                                
CGTGCCCTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCGGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTCCGCCCGCGCCGGGG  >  NC_014034/1253165‑1253361

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG