Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,303,903 Δ1 bp 3.2% intergenic (+90/+67) urtE → / ← RCAP_rcc01231 urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,303,9030G→. 3.2% 5.3 ‑3.9 ‑0.4124intergenic (+90/+67)urtE/RCAP_rcc01231urea ABC transporter ATP‑binding protein UrtE/UbiH/UbiF/VisC/COQ6 family ubiquinone biosynthesis hydroxylase
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (76/44):  total (76/48)
Fisher's exact test strand distribution p-value = 2.07e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.87e-01

Only 100 of 141 total aligned reads displayed.

AGCGTTTAGGCAAGGGGGCGCTG.CCTC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....G....CCAAGATGGGGG..GC.CCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAGCCCGCCCGCAT  >  NC_014034/1303805‑1303996
                                                                                                       |                                                                                                        
aGCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCg                                                                                                          <  2:2251175/101‑1 (MQ=37)
 gCGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCCCCCCCGGGGTATTTGGGGCAAGAT.AAAC.CCCCTCCTTTCACCTTGCCGCAAATATCCCgg                                                                                                         >  2:4200577/1‑101 (MQ=25)
  cGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggg                                                                                                        <  2:1935375/101‑1 (MQ=37)
  cGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggg                                                                                                        >  2:3450970/1‑101 (MQ=25)
  cGTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGAGATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggg                                                                                                        >  1:3764368/1‑101 (MQ=37)
   gTTTAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCgggg                                                                                                       <  1:2713752/101‑1 (MQ=37)
    tttAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCTCCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggggg                                                                                                      <  1:3110089/101‑1 (MQ=37)
    tttAGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCggggg                                                                                                      <  2:2141317/101‑1 (MQ=37)
       cggacggggggCTCTGCCCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGAT.GAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAAt                                                                                                <  1:3652129/95‑1 (MQ=2)
       aGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCCCCTTGCCGCAAATATCCCGGGGGGTg                                                                                                   <  2:2817443/101‑1 (MQ=25)
       aGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTg                                                                                                   >  1:2803911/1‑101 (MQ=37)
       aGGCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCCGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTg                                                                                                   >  2:3034117/1‑101 (MQ=25)
         gCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGaa                                                                                                 >  2:627859/1‑101 (MQ=25)
         gCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGaa                                                                                                 <  2:2298036/101‑1 (MQ=37)
         gCAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGaa                                                                                                 <  1:2713358/101‑1 (MQ=37)
          cAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAt                                                                                                <  1:2281954/101‑1 (MQ=37)
          cAAGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAt                                                                                                <  2:3835025/101‑1 (MQ=37)
           aaGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                                                               <  2:537103/101‑1 (MQ=37)
           aaGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCCCAAATATCCCGGGGGGTGAAtt                                                                                               >  1:953618/1‑101 (MQ=25)
           aaGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCCGGATATTTGGGGCAAGAT.AAAC.CCCATACCTGTTCCTTGCCACAAATATGCCgggggggcaact                                                                                               >  1:3837157/1‑95 (MQ=12)
           aaGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCACCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAtt                                                                                               <  1:1772363/101‑1 (MQ=25)
            aGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTg                                                                                              <  2:3443337/101‑1 (MQ=37)
            aGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTg                                                                                              <  2:2710675/101‑1 (MQ=37)
            aGGGGGCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAAGTg                                                                                              >  2:3748250/1‑101 (MQ=25)
             gggggCTCTGCCCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGATGAAGG.GGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGgcca                                                                                          <  2:4436892/101‑2 (MQ=2)
             gggggCTCTGCCCCC..CGGCTTCG.CTC.CCCCCGGGATATTTGCGGCAAGAT.GAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGgcca                                                                                          <  2:4155742/101‑2 (MQ=2)
             gggggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTgg                                                                                             >  2:3503051/1‑101 (MQ=37)
             gggggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTgg                                                                                             <  2:337839/101‑1 (MQ=37)
             gggggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAAGATCCCGGGGGGTGAAtgag                                                                                             >  2:2092892/1‑98 (MQ=25)
             gggggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCCGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTgg                                                                                             <  1:3824735/101‑1 (MQ=25)
               gggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGgcc                                                                                           <  2:344965/101‑1 (MQ=37)
               gggCGCTG.CCCCCTCGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGgcc                                                                                           >  1:423240/1‑101 (MQ=37)
                                                      tttGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcg                                                 >  1:3534568/1‑101 (MQ=14)
                                                      tttGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcg                                                 <  1:954819/101‑1 (MQ=14)
                                                       ttGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcgg                                                <  2:4304026/101‑1 (MQ=14)
                                                        tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGTGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                                               >  2:300071/1‑101 (MQ=9)
                                                        tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                                               >  2:2885445/1‑101 (MQ=14)
                                                        tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                                               >  1:3138648/1‑101 (MQ=14)
                                                        tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                                               >  2:3787186/1‑101 (MQ=14)
                                                        tGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGGGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGgggcggg                                               >  2:3016145/1‑101 (MQ=9)
                                                          gggCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggcc                                             <  2:4133103/101‑1 (MQ=14)
                                                          gggCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggcc                                             >  2:4154139/1‑101 (MQ=14)
                                                          gggCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggcc                                             >  2:806173/1‑101 (MQ=14)
                                                          gggCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggcc                                             >  1:3736375/1‑101 (MQ=14)
                                                           ggCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccg                                            >  2:3622874/1‑101 (MQ=14)
                                                             cAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgca                                          >  1:3356076/1‑101 (MQ=14)
                                                             cAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgca                                          >  2:3888972/1‑101 (MQ=14)
                                                              aaGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcag                                         >  1:210835/1‑101 (MQ=14)
                                                               aGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg                                        >  2:3254270/1‑101 (MQ=14)
                                                               aGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg                                        >  1:2649734/1‑101 (MQ=14)
                                                               aGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg                                        <  1:203533/101‑1 (MQ=14)
                                                               aGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagg                                        >  2:1255868/1‑101 (MQ=14)
                                                                gAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcaggg                                       >  2:4423962/1‑101 (MQ=14)
                                                                 aT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggc                                      >  1:93677/1‑101 (MQ=14)
                                                                  t.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggcc                                     <  2:2480909/101‑1 (MQ=14)
                                                                    aaaC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccg                                    >  2:423736/1‑101 (MQ=14)
                                                                    aaaC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccg                                    >  1:1427834/1‑101 (MQ=14)
                                                                     aaC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccgc                                   >  2:1781408/1‑101 (MQ=14)
                                                                     aaC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCgggccgcagggccgc                                   >  1:3966317/1‑101 (MQ=14)
                                                                       c.cccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAc                                 >  1:1227406/1‑101 (MQ=14)
                                                                       c.cccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAc                                 >  2:2303029/1‑101 (MQ=14)
                                                                         cccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAcc                                >  1:2702403/1‑101 (MQ=14)
                                                                         cccATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCAcc                                >  1:486319/1‑101 (MQ=14)
                                                                          ccATCCTGTCACCTGGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTGCGGGGCGGGCCGCAGGGCCGCAccc                               <  1:4417613/101‑1 (MQ=9)
                                                                           cctCCTTTCACCTTGCCGCAAATTTCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCt                              <  2:4031089/99‑1 (MQ=9)
                                                                           cATCCTTTCACCTTGCCTTAAATATCCCGGGGGGTGAACTGGCC....GAAGACCAAGAGGGGGGCAGCGCCCCTTTTTCGGGGGGGGCCGCAGGGCCGCACCCt                              <  2:2638958/101‑1 (MQ=9)
                                                                           cATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCt                              >  2:263089/1‑101 (MQ=14)
                                                                           cATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCt                              <  1:549071/101‑1 (MQ=14)
                                                                             tCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGt                            >  2:1007778/1‑101 (MQ=14)
                                                                             tCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGt                            >  1:453942/1‑101 (MQ=14)
                                                                              ccTTTCACCATGCCGCAAATATCCCGGGGGGTGAATTAGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGACGCCGGGCCGCCCCCTGTc                           >  2:437568/1‑101 (MQ=2)
                                                                               cTTTCACCTTGCCGCAAATGTCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCa                          <  2:4261764/101‑1 (MQ=9)
                                                                               cTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCa                          >  1:288626/1‑101 (MQ=14)
                                                                               cTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCa                          >  2:4376887/1‑101 (MQ=14)
                                                                               cTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCa                          <  2:1670313/101‑1 (MQ=14)
                                                                                 ttCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgc                        >  2:2490301/1‑101 (MQ=14)
                                                                                 ttCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgc                        >  2:1114489/1‑101 (MQ=14)
                                                                                 ttCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgc                        >  1:1608606/1‑101 (MQ=14)
                                                                                  tCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgcg                       >  1:2494327/1‑101 (MQ=14)
                                                                                  tCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAgcg                       <  1:3716282/101‑1 (MQ=14)
                                                                                    aCCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCa                     >  1:4357470/1‑101 (MQ=17)
                                                                                     ccTTGCGGCAAATGTCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt                    <  2:3837152/101‑1 (MQ=12)
                                                                                     ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt                    <  1:4175414/101‑1 (MQ=17)
                                                                                     ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt                    <  1:3936863/101‑1 (MQ=17)
                                                                                     ccTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAt                    <  1:2706238/101‑1 (MQ=17)
                                                                                      cTTGCCGCAAATCTCGCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATc                   <  1:2009981/101‑1 (MQ=12)
                                                                                      cTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCCGCGCCTc                   >  2:665847/1‑101 (MQ=9)
                                                                                      cTTGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATc                   >  1:1216211/1‑101 (MQ=17)
                                                                                       ttGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATcc                  >  2:2808500/1‑101 (MQ=17)
                                                                                        tGCCGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATccc                 >  1:300442/1‑101 (MQ=17)
                                                                                           cGCAAATATCCCGGGGGGTGAATTGGGCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCaa              <  1:2639677/101‑1 (MQ=12)
                                                                                           cGCAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCaa              >  1:2053348/1‑101 (MQ=17)
                                                                                             cAAATATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgc            >  1:667094/1‑101 (MQ=17)
                                                                                              aaaTATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcc           >  2:3635357/1‑101 (MQ=17)
                                                                                              aaaTATCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcc           >  2:833017/1‑101 (MQ=17)
                                                                                                atatCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccg         >  2:4287746/1‑101 (MQ=17)
                                                                                                  atCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCAACCCCCAgcccgcc       >  1:3085756/1‑101 (MQ=12)
                                                                                                   tCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccgccc      >  1:85357/1‑101 (MQ=18)
                                                                                                   tCCCGGGGGGTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAgcccgccc      >  1:2698747/1‑101 (MQ=18)
                                                                                                       ggggggTGAATTGGCCGAAGG....CCAAGAGGGGGGCAGCGCCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAGCCCGCCCGCAt  <  2:3093620/101‑1 (MQ=18)
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AGCGTTTAGGCAAGGGGGCGCTG.CCTC..CGGCTTCGCCTCACCCCCGGGATATTTGGGGCAAGAT.AAAC.CCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCC....G....CCAAGATGGGGG..GC.CCCCCTTTTCGGGGCGGGCCGCAGGGCCGCACCCTGTCAGCGCATCCCCAAGCCCGCCCGCAT  >  NC_014034/1303805‑1303996

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG