BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,891	Δ1 bp	21.3%	intergenic (+703/‑228)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,891	0	G→.	21.3%	181.7 ‑1.0 ‑0.3	249	intergenic (+703/‑228)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (29/24): ref base (91/105): total (120/129)
Fisher's exact test strand distribution p-value = 3.53e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.79e-01

Only 100 of 273 total aligned reads displayed.


GAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1395791‑1395991


gAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..Agcg                                                                                                       >  1:3610022/1‑101 (MQ=255)


         ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.Gt                                                                                             >  1:738949/1‑101 (MQ=255)


         ggcgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.Gt                                                                                             <  2:2244434/101‑1 (MQ=255)


           cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCg                                                                                           >  2:1698917/1‑101 (MQ=255)


           cgTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCg                                                                                           <  2:2380393/101‑1 (MQ=255)


            gTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGc                                                                                          >  2:3779583/1‑101 (MQ=255)


             tGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGcc                                                                                         >  1:818371/1‑100 (MQ=255)


               aGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTcc                                                                                       >  2:4035463/1‑101 (MQ=255)


                   gCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTcccccc                                                                                   <  2:4104911/101‑1 (MQ=255)


                    ccAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCcg                                                                                  >  1:2870650/1‑101 (MQ=255)


                      aGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCcgcg                                                                                >  1:886443/1‑101 (MQ=255)


                            aGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCg                                                                          <  2:351363/101‑1 (MQ=255)


                             gACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGt                                                                         >  1:2010479/1‑101 (MQ=255)


                              aCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTg                                                                        <  1:3700592/101‑1 (MQ=255)


                              aCGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTg                                                                        <  1:2403513/101‑1 (MQ=255)


                                gCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                      >  1:4417930/1‑101 (MQ=255)


                                cggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTC.T..GGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGt                                                                         >  2:4322568/9‑101 (MQ=2)


                                 ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTC.T..GGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTg                                                                        <  1:947105/94‑1 (MQ=2)


                                 ggcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTC.T..GGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTg                                                                        <  1:2816724/94‑1 (MQ=2)


                                 ccGTGGACGCCGCTCCCCCCGCGGGGGCGTAGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTgggt                                                                     >  1:3967150/1‑99 (MQ=255)


                                  gcccggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTC.T..GGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                       >  1:4188640/7‑101 (MQ=2)


                                  aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                    >  2:3835329/8‑101 (MQ=9)


                                  aacctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                    <  1:1730803/94‑1 (MQ=9)


                                   gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                   <  1:1446877/101‑1 (MQ=255)


                                   gTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                   >  2:4161362/1‑101 (MQ=255)


                                     ggACGTCGCTCCCCCCGGGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                 <  1:3197172/101‑1 (MQ=255)


                                     ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCgca                                                                 >  2:3153033/5‑99 (MQ=9)


                                     ctatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                 <  2:398386/97‑1 (MQ=9)


                                      tatgTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                >  1:1374105/4‑101 (MQ=9)


                                      gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                <  1:3000959/101‑1 (MQ=255)


                                      gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                <  2:3453086/101‑1 (MQ=255)


                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                                             <  2:1438586/101‑1 (MQ=255)


                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGa                                                             <  2:1547513/101‑1 (MQ=255)


                                         gTCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaa                                                             >  1:2214862/1‑99 (MQ=9)


                                          tCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt                                                            <  2:1115217/101‑1 (MQ=9)


                                          tCGCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAt                                                            <  1:3458226/101‑1 (MQ=9)


                                           cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATg                                                           <  2:3327359/101‑1 (MQ=9)


                                           cGCCCCCCCCGGGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGG...TCTGCTGGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAca                                                              <  2:2297876/101‑2 (MQ=2)


                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGc                                                          >  2:3363969/1‑101 (MQ=255)


                                            gCTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcactgc                                                          >  1:1579219/1‑96 (MQ=9)


                                             cTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCg                                                         >  1:640151/1‑101 (MQ=255)


                                             cTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                                         >  1:3246038/1‑101 (MQ=9)


                                             cTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                                         <  2:1171247/101‑1 (MQ=9)


                                             cTCACCCCGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCg                                                         >  1:2140371/1‑101 (MQ=9)


                                              tcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                                        >  2:1848907/4‑100 (MQ=9)


                                              tcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                                        >  1:1886760/4‑100 (MQ=9)


                                              tcaccccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCgg                                                        >  1:1013085/4‑100 (MQ=9)


                                               ccccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGtt                                                       <  1:509517/101‑1 (MQ=255)


                                                 ccccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCa                                                     <  2:818289/101‑1 (MQ=255)


                                                  cccGCGGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcg                                                    >  1:1269005/1‑96 (MQ=9)


                                                  cccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                                    >  2:777421/1‑101 (MQ=255)


                                                  cccGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCAt                                                    <  2:1348696/101‑1 (MQ=255)


                                                   ccGCGGGGGCGTGGATCGAAACCCCGCGGCGTCCCGAAGACGGTCTTC.T..GGTGCCGCTGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcaccccgaac                                                      >  2:3681545/1‑92 (MQ=2)


                                                    cgggggggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                                                  <  1:2455606/98‑1 (MQ=255)


                                                    cgcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcggg                                                  >  1:2742254/1‑94 (MQ=2)


                                                    cgcgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                                                  <  1:2921990/101‑1 (MQ=255)


                                                    cgcgGGGGCGTGGACCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATgg                                                  <  2:2941580/101‑1 (MQ=255)


                                                     gcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgggggc                                                 >  2:3028904/1‑93 (MQ=2)


                                                     gcgGGGGCGTGGATCGAAACTGTGCCGGTTCCCGGAGACGC...TG.G..GGC.CCTCGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAATGCggcgcgggc                                                 <  1:2605373/101‑9 (MQ=2)


                                                      cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                                >  2:569951/1‑101 (MQ=255)


                                                      cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                                >  2:805355/1‑101 (MQ=255)


                                                      cgGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGcc                                                <  2:2694760/101‑1 (MQ=255)


                                                         gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                             <  1:1245223/101‑1 (MQ=255)


                                                         gggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTAc                                             <  1:1103834/101‑1 (MQ=255)


                                                          ggCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACg                                            >  2:911430/1‑101 (MQ=255)


                                                             gTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCg                                         >  2:3723964/1‑101 (MQ=255)


                                                              tGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                                        >  2:4306281/1‑101 (MQ=255)


                                                              tGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCgg                                        <  1:3760600/101‑1 (MQ=255)


                                                               ggATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                                       >  1:686999/1‑101 (MQ=255)


                                                               ggATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCggg                                       <  1:4035720/101‑1 (MQ=255)


                                                                   cGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                                   <  1:308008/101‑1 (MQ=255)


                                                                   cGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGt                                   <  1:1940615/101‑1 (MQ=255)


                                                                    gAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTg                                  >  2:3796798/1‑101 (MQ=255)


                                                                     aaaCTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTgg                                 <  2:2849441/101‑1 (MQ=255)


                                                                        cTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACt                              >  2:4061802/1‑101 (MQ=255)


                                                                         tGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg                             <  2:2790534/101‑1 (MQ=255)


                                                                         tGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTg                             <  1:1146497/101‑1 (MQ=255)


                                                                          gcgcCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg                            <  2:2582519/101‑1 (MQ=255)


                                                                          gcaccgccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTgg                            >  1:2563201/4‑101 (MQ=255)


                                                                            gccgccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGGGTTCATGGCCTACCTCGGGCAGTGGACTGGTc                          >  2:2446176/1‑101 (MQ=255)


                                                                            gccgccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc                          >  1:3359068/1‑101 (MQ=255)


                                                                            gccgccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc                          <  1:1806193/101‑1 (MQ=255)


                                                                            gccgccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTc                          <  1:1950262/101‑1 (MQ=255)


                                                                             ccgccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCg                         >  1:3171844/1‑101 (MQ=255)


                                                                               gccTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCCTGGCCTACGTCGGGCAGCGGACTGGGCGCt                       >  1:2297049/1‑101 (MQ=255)


                                                                                  tATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGTGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc                    >  2:2046569/1‑101 (MQ=255)


                                                                                  tATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccc                    <  2:1523071/101‑1 (MQ=255)


                                                                                    tCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTccccc                  <  1:4137925/101‑1 (MQ=255)


                                                                                     cGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc                 >  2:282878/1‑101 (MQ=255)


                                                                                     cGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTcccccc                 <  1:2970611/101‑1 (MQ=255)


                                                                                      gCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcg                >  2:260335/1‑101 (MQ=255)


                                                                                      ccATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCcg                <  2:4437812/100‑1 (MQ=255)


                                                                                          ccGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggg            >  2:1261852/1‑101 (MQ=255)


                                                                                           cGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCgggg           <  2:234122/101‑1 (MQ=255)


                                                                                            gg...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCggggg          >  1:2433647/1‑101 (MQ=255)


                                                                                                       aGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGGCGCTCCCCCCGCGGGGGCGTgg     >  2:1962108/1‑101 (MQ=255)


                                                                                                         cgccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGAt   >  1:2696060/1‑101 (MQ=255)


                                                                                                         cgccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGCGGACTGGTCGCTCCCCCCGCGGGGGCGTGGAt   >  2:3001654/1‑101 (MQ=255)


                                                                                                          gccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTTCGTCGGGCAGGTGACTGGTCGATCCCCCCGCGGGGGCGTGGAtt  <  2:2563038/101‑2 (MQ=37)


                                                                                                          gccgcGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATc  >  2:3890713/1‑101 (MQ=255)


GAAACCTGCGGCGTGAGTTGCCAGTGATAGACGCCGTGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGG...TC.G..AGCGCCGCGCA.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATGCGTTCATGGCCTACGTCGGGCAGTGGACTGGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1395791‑1395991

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG