Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,166 Δ1 bp 3.9% coding (48/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,1660C→. 3.9% 14.2 ‑0.8 ‑1.0179coding (48/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/5):  ref base (82/88):  total (86/93)
Fisher's exact test strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.09e-01

Only 100 of 219 total aligned reads displayed.

GCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1396066‑1396266
                                                                                                       |                                                                                                    
gcgcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGc                                                                                                      <  1:3417775/101‑1 (MQ=255)
  gcATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGt                                                                                                    >  2:1760031/1‑101 (MQ=255)
   cATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCCCCACG.CTGCGTc                                                                                                   >  1:938107/1‑101 (MQ=255)
    aTCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCg                                                                                                  >  2:4092765/1‑101 (MQ=255)
    aTCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCg                                                                                                  >  2:2475520/1‑101 (MQ=255)
     tCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCG.CCGCGTCGc                                                                                                 >  1:1022697/1‑101 (MQ=255)
     tCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGc                                                                                                 >  2:3630665/1‑101 (MQ=255)
         aTGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGCCGCTccc                                                                                             >  1:3897358/1‑101 (MQ=255)
          tGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGGCACG.CTGCGTCGCTcccc                                                                                            >  2:470058/1‑101 (MQ=255)
          tGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTcccc                                                                                            <  2:507125/101‑1 (MQ=255)
              tatGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CCGGGTCGCTCCCCCcgc                                                                                        >  2:284957/1‑101 (MQ=255)
               atGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCC.CCGCGGCCCCCCCCCcccg                                                                                       >  1:892226/1‑101 (MQ=25)
               atGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCCCCCCCcgcg                                                                                       >  1:3191280/1‑101 (MQ=255)
                 gCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCggg                                                                                     >  1:647074/1‑101 (MQ=255)
                  cccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCgggg                                                                                    >  2:3591668/1‑101 (MQ=255)
                  cccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCgggg                                                                                    <  2:1655156/101‑1 (MQ=255)
                  cccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CCGCGGCGCTCCCCCCGCgggg                                                                                    >  2:925479/1‑101 (MQ=255)
                   ccGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCggggg                                                                                   >  1:2857460/1‑101 (MQ=255)
                    nggTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTcccccccnnnngnn                                                                                  >  1:726035/2‑93 (MQ=255)
                     ggTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCg                                                                                 <  2:3679738/101‑1 (MQ=255)
                        gtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCG.CTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                              <  1:599804/92‑1 (MQ=2)
                        gtctgcgcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCCTCGATCCGGTCAGCCTCGTATTG.CGCG.CTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                              <  2:3899311/92‑1 (MQ=2)
                        gagaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCCGGGGCGGgg                                                                              >  2:1499785/1‑101 (MQ=255)
                         agaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCTCCGCCACG.CTCCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                             >  2:2180607/1‑101 (MQ=255)
                         agaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTggg                                                                             <  2:1590442/101‑2 (MQ=255)
                          gaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                            <  1:2046706/101‑1 (MQ=255)
                          gaCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACC.CTGCGTCGCTCCCCCCCCGGGGGCGGgggt                                                                            >  1:1716904/1‑99 (MQ=255)
                           aCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                           <  1:2475677/101‑1 (MQ=255)
                           aCGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                           <  2:3091917/101‑1 (MQ=255)
                             ggCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                         >  1:2187927/1‑101 (MQ=255)
                               cTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                       <  1:537105/101‑1 (MQ=255)
                               cTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                       >  1:4268349/1‑101 (MQ=255)
                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGGGGGGATCGCAAcc                                                                     >  1:4202618/1‑101 (MQ=255)
                                 gTCGCTCCCCCCGCGGGGGCGCGGATCGAAACTTTTCGCC.AACTCG.CAGGCCCGCCCCCCCACGCCTGC.CCTCTCCCTCCGGGGGGCGGTGGATGGGAAcc                                                                     >  2:2366674/1‑101 (MQ=9)
                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAACCg                                                                   >  2:3905634/1‑101 (MQ=255)
                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGC.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCg                                                                   <  1:819524/101‑1 (MQ=255)
                                     cTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCCCG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGc                                                                 <  1:190951/101‑1 (MQ=255)
                                     cTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGc                                                                 <  2:821189/101‑1 (MQ=255)
                                        cccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGaa                                                              <  1:1004755/101‑1 (MQ=255)
                                         ccccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGaaa                                                             >  2:4298928/1‑101 (MQ=255)
                                          cccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGaaaa                                                            >  1:329609/1‑101 (MQ=255)
                                          cccGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGaaaa                                                            <  1:3552597/101‑1 (MQ=255)
                                            cgcgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAAc                                                          <  2:4191125/101‑1 (MQ=255)
                                            cgcgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAAc                                                          <  2:3358831/101‑1 (MQ=255)
                                              cgGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACgg                                                        >  1:28233/1‑101 (MQ=255)
                                               gggggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTTGATCGAAACCCGGGGAAAAACGGc                                                       >  2:4216315/1‑101 (MQ=255)
                                                  ggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCAt                                                    <  2:2010350/101‑1 (MQ=255)
                                                  ggCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCAt                                                    <  2:534412/101‑1 (MQ=255)
                                                   gCGTGGATCGAGACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATg                                                   <  1:1276218/101‑1 (MQ=255)
                                                   gCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATg                                                   <  1:1633590/101‑1 (MQ=255)
                                                    cGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGc                                                  >  2:3567995/1‑101 (MQ=255)
                                                      tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCt                                                <  2:1169106/101‑1 (MQ=255)
                                                      tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCt                                                <  1:2366345/101‑1 (MQ=255)
                                                      tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCt                                                <  1:3084517/101‑1 (MQ=255)
                                                      tGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATACCt                                                >  2:2973448/1‑101 (MQ=255)
                                                        gATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGGCATGCCCcc                                              >  2:862384/1‑101 (MQ=255)
                                                         aTCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCg                                             >  2:1709740/1‑101 (MQ=255)
                                                         aTCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCg                                             >  1:4071857/1‑101 (MQ=255)
                                                          tCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCgg                                            >  2:2634794/1‑101 (MQ=255)
                                                           cGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCggg                                           <  1:470110/101‑1 (MQ=255)
                                                                cTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCt                                      <  2:256763/101‑1 (MQ=255)
                                                                 ttttCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTg                                     <  1:985873/101‑1 (MQ=255)
                                                                 ttttCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTg                                     <  1:1537340/101‑1 (MQ=255)
                                                                 ttttCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTg                                     >  1:3509715/1‑101 (MQ=255)
                                                                    tCGTC.ATCTGG.CAGGCCCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCt                                  <  2:3280247/101‑1 (MQ=255)
                                                                     cGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTg                                 >  1:3963506/1‑101 (MQ=255)
                                                                     cGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGcgg                                 <  2:2884333/101‑3 (MQ=255)
                                                                     cGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGGGTCTGGCTg                                 >  2:3071407/1‑101 (MQ=255)
                                                                        c.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCg                              <  2:1077507/101‑1 (MQ=255)
                                                                        c.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCg                              >  1:2293696/1‑101 (MQ=255)
                                                                          aTCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGc                             >  2:2496218/1‑101 (MQ=255)
                                                                             tGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcc                          >  2:2288615/1‑101 (MQ=255)
                                                                              gg.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTccc                         <  1:1979263/101‑1 (MQ=255)
                                                                               g.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcccc                        <  2:2857252/101‑1 (MQ=255)
                                                                                  aGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcccccc                      <  1:756955/101‑1 (MQ=255)
                                                                                  aGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTcccccc                      >  1:3435522/1‑101 (MQ=255)
                                                                                      tCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCCCCCCCCGCgg                  >  2:2787442/1‑101 (MQ=255)
                                                                                       cgcgCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCggg                 >  2:3126632/1‑101 (MQ=255)
                                                                                         cgccgcCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCggggg               >  2:195935/1‑101 (MQ=255)
                                                                                         cgccgcCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCggggg               >  1:2582251/1‑101 (MQ=255)
                                                                                          gccgccACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGc              <  2:91696/101‑1 (MQ=255)
                                                                                          gccgccACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGc              <  2:1379935/101‑1 (MQ=255)
                                                                                          gccgccACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGc              >  1:122336/1‑101 (MQ=255)
                                                                                            cgccACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGt            >  2:1551611/1‑101 (MQ=255)
                                                                                            cgccACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGt            <  2:3973900/101‑1 (MQ=255)
                                                                                             gccACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTg           <  1:1499890/101‑1 (MQ=255)
                                                                                               cACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTGGa         <  1:2450867/101‑1 (MQ=255)
                                                                                               cACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTGGa         >  2:1601373/1‑101 (MQ=255)
                                                                                                ccG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTGGAt        <  1:2137896/100‑1 (MQ=255)
                                                                                                aCG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTGGAt        >  2:2809425/1‑101 (MQ=255)
                                                                                                aCG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTGGAt        >  1:743118/1‑101 (MQ=255)
                                                                                                 cG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTGGATc       <  2:1716793/101‑1 (MQ=255)
                                                                                                 cG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTGGATc       <  1:1134790/101‑1 (MQ=255)
                                                                                                  g.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCg      <  2:3922225/101‑1 (MQ=255)
                                                                                                  g.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCAATGCCTCCGGGTGGCTGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCg      >  2:1682633/1‑101 (MQ=255)
                                                                                                     tGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGaa    >  1:3762377/1‑101 (MQ=255)
                                                                                                      gCGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACCCGGTGAAAAACGGCCATGCCTCCGGGTGCCTGGCCGTCGCTCCCCCCCCGGGGGCGTGGATCGaaa   >  1:1206639/1‑101 (MQ=38)
                                                                                                      gCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa   <  2:3571339/101‑1 (MQ=255)
                                                                                                       cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc  <  2:2175910/101‑1 (MQ=255)
                                                                                                       cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAc  >  2:3783444/1‑101 (MQ=255)
                                                                                                       |                                                                                                    
GCGCATCTGATGTATATGCCCGGTGAGACGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTTTCGTC.ATCTGG.CAGGCTCGCGCCGCCACG.CTGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGGCGAAAAACGGCCATGCCTCCGGGTGTCTGGCTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1396066‑1396266

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG