BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,396,935	Δ1 bp	14.7%	coding (817/1578 nt)	RCAP_rcc01307 →	hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,396,935	0	A→.	14.7%	167.2 ‑2.3 ‑0.4	369	coding (817/1578 nt)	RCAP_rcc01307	hypothetical protein
Reads supporting (aligned to +/- strand): new base (34/20): ref base (151/147): total (190/179)
Fisher's exact test strand distribution p-value = 1.05e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.85e-01

Only 100 of 386 total aligned reads displayed.


GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGT  >  NC_014034/1396835‑1397035


     cTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.g                                                                                                          <  1:4278828/101‑1 (MQ=255)


     cTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.g                                                                                                          <  1:2705227/101‑1 (MQ=255)


      tCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.gc                                                                                                         <  1:1747079/101‑1 (MQ=255)


       ccccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.gcg                                                                                                        >  1:3082486/1‑101 (MQ=255)


       ccccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.ACCT.gcg                                                                                                        >  2:1878981/1‑101 (MQ=255)


              cgGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.TCCT.GCGCG.GC.CGc                                                                                               >  1:3831743/1‑101 (MQ=255)


              cgGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGc                                                                                               >  1:2939789/1‑101 (MQ=255)


              cgGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGc                                                                                               >  1:2595193/1‑101 (MQ=255)


               gggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCa                                                                                              <  2:1961925/101‑1 (MQ=255)


               gggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCa                                                                                              >  2:2655218/1‑101 (MQ=255)


               gggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCa                                                                                              >  2:465649/1‑101 (MQ=255)


                ggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.t                                                                                            <  2:77436/101‑1 (MQ=255)


                    cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGc                                                                                        >  1:14634/1‑101 (MQ=255)


                      tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCt.t                                                                                     >  2:3586799/1‑101 (MQ=255)


                          tCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.c                                                                                >  1:2776605/1‑101 (MQ=255)


                            gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.Ct                                                                             >  1:245020/1‑101 (MQ=255)


                             aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTg                                                                            >  2:318769/1‑101 (MQ=255)


                             aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTg                                                                            >  2:2104088/1‑101 (MQ=255)


                               aCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..Cg                                                                        <  1:1370727/101‑1 (MQ=255)


                                 cGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTg                                                                      >  1:1571533/1‑101 (MQ=255)


                                 cGGTCCAGAGAGAGACCGCGCGGCCCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTg                                                                      <  2:4421564/101‑1 (MQ=255)


                                  ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTg.g                                                                    <  2:55069/101‑1 (MQ=255)


                                    tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTc                                                                  <  2:953218/101‑1 (MQ=255)


                                       agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCt                                                               <  1:1781672/101‑1 (MQ=255)


                                        gagaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTc                                                              <  2:3834154/101‑1 (MQ=255)


                                           aTAGACCGCGCGGCCCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTcccc                                                           <  1:3903085/101‑1 (MQ=255)


                                            tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTccccc                                                          <  1:1212332/101‑1 (MQ=255)


                                            tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTccccc                                                          >  1:3333695/1‑101 (MQ=255)


                                              gACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCcg                                                        <  1:4099176/101‑1 (MQ=255)


                                                   cgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCgggg                                                   <  1:3551321/101‑1 (MQ=255)


                                                    gcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCggggg                                                  >  1:1047280/1‑101 (MQ=255)


                                                          ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                             <  2:619402/91‑1 (MQ=9)


                                                           ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                           <  2:3119270/92‑1 (MQ=2)


                                                           tcctcAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                           <  1:4221095/101‑1 (MQ=255)


                                                           gtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGa                                            >  1:100646/10‑101 (MQ=9)


                                                            tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                          >  1:2274427/9‑101 (MQ=2)


                                                            tcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                           <  1:982790/93‑1 (MQ=9)


                                                            cctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                          >  2:2124760/1‑101 (MQ=255)


                                                            acccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTgg                                             <  1:550935/94‑1 (MQ=0)


                                                             ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                         <  1:2994627/94‑1 (MQ=2)


                                                             ctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                         >  1:2826252/1‑101 (MQ=255)


                                                             caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                          >  1:3953331/8‑101 (MQ=9)


                                                              tcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                        >  1:3655865/1‑101 (MQ=255)


                                                              tcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                        >  1:938767/1‑101 (MQ=255)


                                                              ggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                         >  2:4317839/6‑101 (MQ=2)


                                                              gccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                        <  1:3118427/95‑1 (MQ=2)


                                                              aacactgTCGCGCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                         <  1:3713091/95‑1 (MQ=9)


                                                                cccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      >  2:3522513/5‑101 (MQ=2)


                                                                 tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGGGGGTCGaaa                                      >  2:3763312/3‑101 (MQ=2)


                                                                 actgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                      >  2:1092220/4‑101 (MQ=9)


                                                                  ctgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                     >  1:1853269/3‑101 (MQ=9)


                                                                  cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGAG.CTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                    <  1:835095/99‑2 (MQ=2)


                                                                  aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                    >  1:4414090/1‑101 (MQ=255)


                                                                   tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                    <  2:1330382/100‑2 (MQ=9)


                                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                 >  1:2392147/1‑101 (MQ=255)


                                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActaca                                 <  1:3233515/101‑5 (MQ=2)


                                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                   <  2:2341368/101‑2 (MQ=2)


                                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                 >  2:2511067/1‑97 (MQ=9)


                                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                 >  2:3791207/1‑97 (MQ=9)


                                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGAGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAActcgg                                 >  2:694300/1‑97 (MQ=9)


                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                               >  2:1767107/1‑101 (MQ=255)


                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                               <  1:1925187/101‑7 (MQ=2)


                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                <  1:215334/101‑6 (MQ=9)


                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                >  2:1732173/1‑96 (MQ=9)


                                                                       gCTCCCCCCGCGGGGGCGTGGAGCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GGCGCTCCCCCCGCGGGGGCGTGGATCGGAActacaac                               >  2:4167770/1‑95 (MQ=2)


                                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCa                                 >  1:1117362/1‑101 (MQ=2)


                                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTCGATCGAAACACCa                                 >  2:3707640/1‑101 (MQ=2)


                                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCt                              >  2:1186468/1‑101 (MQ=255)


                                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccg                              >  1:19824/1‑94 (MQ=2)


                                                                        cTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcc                               >  2:2015990/1‑95 (MQ=9)


                                                                         tCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcccc                              >  2:3383085/1‑94 (MQ=2)


                                                                         tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                             <  2:14609/101‑1 (MQ=255)


                                                                         tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                              <  1:3773961/101‑8 (MQ=9)


                                                                         cccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                             <  1:3585781/100‑1 (MQ=255)


                                                                          ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                             <  2:3466092/101‑9 (MQ=9)


                                                                           cccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgggg                           >  1:2316220/1‑91 (MQ=2)


                                                                             cccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgcc                            <  1:1610809/101‑6 (MQ=0)


                                                                             cccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtcg                         <  2:3300753/101‑1 (MQ=255)


                                                                             cccGCGGGGGCGTGGATCCAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtcg                         <  1:1688655/101‑1 (MQ=255)


                                                                              ccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                           >  2:1654479/1‑95 (MQ=0)


                                                                                gcgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTg                      <  1:4058159/101‑1 (MQ=255)


                                                                                  gggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCCCGTCGTTGGt                    >  1:4154273/1‑101 (MQ=255)


                                                                                   ggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTCAGCCCGTCGTTGGTc                   >  1:496151/1‑101 (MQ=255)


                                                                                    gggGGTGGATGGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                  <  1:2083712/101‑1 (MQ=255)


                                                                                    gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                  <  2:852037/101‑1 (MQ=255)


                                                                                     ggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAg                 <  2:2915808/101‑1 (MQ=255)


                                                                                     ggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAg                 <  2:1571428/101‑1 (MQ=255)


                                                                                      gCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGc                <  2:4080902/101‑1 (MQ=255)


                                                                                        gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGGCAGCCg              >  1:743041/1‑101 (MQ=255)


                                                                                         tGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg             >  1:1326538/1‑101 (MQ=255)


                                                                                         tGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg             >  2:2438183/1‑101 (MQ=255)


                                                                                           gATCGAACCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc           <  2:2826045/101‑1 (MQ=255)


                                                                                            aTCCAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg          <  1:2017254/101‑1 (MQ=255)


                                                                                             tCGAAACA.GCCT.GCGCG.GC.CGCA.GCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGa         <  2:1427406/101‑1 (MQ=255)


                                                                                              cGAAACA.GCCT.GCGCG.GC.CGCA.ACGGCG.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGCGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGAt        >  2:2216598/1‑101 (MQ=255)


                                                                                              cGAAACA.GCCT.GCCCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGAt        <  1:3425929/101‑1 (MQ=255)


                                                                                               gAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATg       >  2:3908200/1‑101 (MQ=255)


                                                                                                 aaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTc     <  2:2391997/101‑1 (MQ=255)


                                                                                                  aCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc    <  1:638436/101‑1 (MQ=255)


                                                                                                    a.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGt  <  2:3988085/101‑1 (MQ=255)


GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGT  >  NC_014034/1396835‑1397035

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 25 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG