Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_014034 | 1,696,217 | Δ1 bp | 100% | intergenic (‑145/+80) | RCAP_rcc01564 ← / ← mmsA | isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase |
Read alignment evidence... | ||||||||||
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seq id | position | change | freq | score | reads | annotation | genes | product | ||
* | NC_014034 | 1,696,217 | 0 | C→. | 100.0% | 80.2 | 138 | intergenic (‑145/+80) | RCAP_rcc01564/mmsA | isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase |
Reads supporting (aligned to +/- strand): new base (54/60): ref base (9/15): total (63/75) | ||||||||||
Fisher's exact test strand distribution p-value = 5.00e-01 | ||||||||||
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.81e-03 |
Only 100 of 146 total aligned reads displayed.
GCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCGG.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTCGAGCGCCTTGAA > NC_014034/1696124‑1696314
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gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc > 1:765725/1‑101 (MQ=255)
cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt < 1:2635474/101‑1 (MQ=255)
cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg < 1:923827/101‑1 (MQ=255)
cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg < 1:869891/101‑1 (MQ=255)
gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg < 2:889481/101‑1 (MQ=255)
gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg < 2:1074620/101‑1 (MQ=255)
aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc < 2:117410/101‑1 (MQ=9)
aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc < 1:4426896/101‑1 (MQ=9)
ttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGGTGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc > 1:1685177/1‑101 (MQ=9)
ttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc > 2:3959138/1‑101 (MQ=9)
gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaac < 2:2236158/101‑2 (MQ=9)
gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt < 2:3218898/101‑1 (MQ=9)
cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa < 1:1991875/101‑1 (MQ=255)
aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg > 2:438421/1‑101 (MQ=255)
ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc < 1:3694104/101‑1 (MQ=255)
gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc < 1:1354249/101‑1 (MQ=255)
gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc > 1:2156471/1‑101 (MQ=9)
gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc < 1:997578/101‑1 (MQ=255)
aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct < 1:3959394/101‑1 (MQ=9)
ggggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctc < 2:156628/101‑1 (MQ=255)
gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct > 1:3065993/1‑101 (MQ=9)
gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct < 2:4207554/101‑1 (MQ=255)
gaggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc < 2:3136530/92‑1 (MQ=9)
gaggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc < 1:4168945/92‑1 (MQ=9)
aggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc < 2:4291935/93‑1 (MQ=255)
aggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc < 1:1182693/93‑1 (MQ=255)
aggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc < 1:4241725/93‑1 (MQ=255)
gggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGa > 1:2642652/7‑101 (MQ=16)
gggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGa < 2:1330376/95‑1 (MQ=12)
ggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGaa < 2:3561214/96‑1 (MQ=255)
gcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg > 2:2845779/5‑101 (MQ=16)
gcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg < 1:2348519/97‑1 (MQ=255)
cgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg < 2:3560932/98‑1 (MQ=16)
cgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg < 2:369306/98‑1 (MQ=16)
cgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg > 1:311941/4‑101 (MQ=16)
cgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg > 1:1837180/4‑101 (MQ=16)
ctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc > 1:1511444/2‑101 (MQ=16)
ctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc > 2:1163031/2‑101 (MQ=16)
tGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa > 2:3732371/1‑101 (MQ=19)
tGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa > 1:60797/1‑101 (MQ=19)
tGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa > 2:132586/1‑101 (MQ=255)
tGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa < 1:1792210/101‑1 (MQ=16)
ccccTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg < 2:3371320/101‑1 (MQ=255)
ccccTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg < 2:2569006/101‑1 (MQ=255)
ccccTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg < 1:4247761/101‑1 (MQ=255)
cccTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGc < 2:628199/101‑1 (MQ=16)
cTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGCGCCAAATACGCCTCTGGCCCGAAGGGCaaccgc > 2:1734377/1‑97 (MQ=12)
tcctGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcgc > 2:3470728/4‑101 (MQ=255)
cctGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcgcg > 1:4355323/3‑101 (MQ=19)
cctGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcgag > 2:2059394/3‑99 (MQ=19)
ctGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCgcgc > 2:1777345/2‑101 (MQ=19)
ctGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCgagc > 2:549434/2‑101 (MQ=16)
tGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGcc > 1:2792553/1‑101 (MQ=255)
ggCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg > 2:3266173/1‑101 (MQ=255)
ggCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg > 1:4136000/1‑101 (MQ=255)
gCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGa < 1:2173129/101‑1 (MQ=21)
ccTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa < 1:255591/101‑1 (MQ=19)
ccTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa < 1:235663/101‑1 (MQ=19)
ttCGGCCAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgc < 2:3417890/101‑1 (MQ=25)
ttCGGCCAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgc < 1:1594419/101‑1 (MQ=25)
ttCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgc < 1:4156747/101‑1 (MQ=21)
ttCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgc < 2:3656425/101‑1 (MQ=19)
tcggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcg < 1:761875/96‑1 (MQ=19)
cggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcgc > 1:3464967/5‑101 (MQ=255)
ggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGcgcg > 2:3793398/4‑101 (MQ=255)
cAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcc > 1:1977741/1‑101 (MQ=255)
attcaccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcccc > 1:3922103/7‑101 (MQ=255)
ttcaccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccccc < 2:3515115/96‑1 (MQ=25)
tcaccccGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATCCGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcccccc < 1:1104200/97‑1 (MQ=255)
tcaccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcccccc < 1:3490927/97‑1 (MQ=25)
tcaccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcccccc > 1:455551/5‑101 (MQ=255)
ccccccGGGAGGTTTGGGCC..AGGGTG.AACC.CCCTTTTTTTTTCTTGGCCCAAATCCGCCCCTGCCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCt < 1:194938/98‑1 (MQ=255)
caccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCt > 2:1572447/4‑101 (MQ=255)
accccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTg > 1:2293115/3‑101 (MQ=255)
cccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGAt > 1:2230993/1‑101 (MQ=255)
ccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATc > 2:4087160/1‑101 (MQ=255)
ccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATc > 2:2190759/1‑101 (MQ=255)
ccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAAGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATc < 1:3623009/101‑1 (MQ=255)
cGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCg > 2:3938572/1‑101 (MQ=255)
cGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCg > 1:2412738/1‑101 (MQ=255)
gggATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGt < 2:1660085/101‑1 (MQ=37)
ggatgtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTg > 1:1091621/7‑101 (MQ=255)
atgtttGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGcc < 1:3266405/97‑1 (MQ=37)
tgtttGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCt < 1:4292402/98‑1 (MQ=37)
tgtttGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCGGGCCCGAGGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCt < 2:1091784/98‑1 (MQ=25)
tgtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCt > 1:4334890/4‑101 (MQ=255)
tgtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCt > 2:4057694/4‑101 (MQ=255)
tttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCa > 1:2836101/2‑101 (MQ=255)
tttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCa > 1:279279/2‑101 (MQ=255)
tttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCa > 2:3960395/2‑101 (MQ=255)
tttGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCa > 1:165109/2‑101 (MQ=255)
ttGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAg < 2:204152/101‑1 (MQ=38)
tggtccaaggTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATGCGCCTCTGCCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGt < 2:1963303/93‑1 (MQ=255)
gggccaaggTGAAAC..CCCTTGTTTTTTCTTGGCGCAAATACGCCTCTGGCCGGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTc < 2:490526/94‑1 (MQ=255)
gggccaaggTGAAAC..CCCTTGCTTTTTCTTGGCGAAAATCCGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTc < 2:1991471/94‑1 (MQ=255)
caaaggTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATCCGCCTCGGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTCGAg < 2:1896349/97‑1 (MQ=255)
caaggTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTCGAgc > 1:1439518/4‑101 (MQ=255)
aggTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTCGAgcgc > 1:3389947/2‑101 (MQ=255)
tgaacccccTTGCTTTTTCTTGGCGAAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTCGAGCGCCtt < 2:4408392/95‑1 (MQ=255)
aaccccTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTCGAGCGCCTTGaa < 2:165088/98‑1 (MQ=255)
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GCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCGG.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTCGAGCGCCTTGAA > NC_014034/1696124‑1696314
Base quality scores: 
ATCG
< 3 ≤
ATCG
< 18 ≤
ATCG
< 27 ≤
ATCG
< 34 ≤
ATCG
< 41 ≤
ATCG
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