Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,696,217 Δ1 bp 100% intergenic (‑145/+80) RCAP_rcc01564 ← / ← mmsA isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,696,2170C→.100.0% 80.2 138intergenic (‑145/+80)RCAP_rcc01564/mmsAisobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  new base (54/60):  ref base (9/15):  total (63/75)
Fisher's exact test strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.81e-03

Only 100 of 146 total aligned reads displayed.

GCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCGG.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTCGAGCGCCTTGAA  >  NC_014034/1696124‑1696314
                                                                                              |                                                                                                 
gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                                                       >  1:765725/1‑101 (MQ=255)
 cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt                                                                                      <  1:2635474/101‑1 (MQ=255)
   cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                                                                    <  1:923827/101‑1 (MQ=255)
   cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                                                                    <  1:869891/101‑1 (MQ=255)
    gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                                                                                   <  2:889481/101‑1 (MQ=255)
    gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                                                                                   <  2:1074620/101‑1 (MQ=255)
     aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                                                                                  <  2:117410/101‑1 (MQ=9)
     aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                                                                                  <  1:4426896/101‑1 (MQ=9)
       ttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGGTGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc                                                                                >  1:1685177/1‑101 (MQ=9)
       ttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc                                                                                >  2:3959138/1‑101 (MQ=9)
           gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaac                                                                            <  2:2236158/101‑2 (MQ=9)
           gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                                                            <  2:3218898/101‑1 (MQ=9)
            cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                                           <  1:1991875/101‑1 (MQ=255)
              aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                                                         >  2:438421/1‑101 (MQ=255)
               ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                                                                        <  1:3694104/101‑1 (MQ=255)
                gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                                                       <  1:1354249/101‑1 (MQ=255)
                gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                                                       >  1:2156471/1‑101 (MQ=9)
                gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                                                       <  1:997578/101‑1 (MQ=255)
                 aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                                                      <  1:3959394/101‑1 (MQ=9)
                  ggggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctc                                                                     <  2:156628/101‑1 (MQ=255)
                   gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                                                    >  1:3065993/1‑101 (MQ=9)
                   gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                                                    <  2:4207554/101‑1 (MQ=255)
                         gaggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                                                                <  2:3136530/92‑1 (MQ=9)
                         gaggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                                                                <  1:4168945/92‑1 (MQ=9)
                          aggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc                                                               <  2:4291935/93‑1 (MQ=255)
                          aggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc                                                               <  1:1182693/93‑1 (MQ=255)
                          aggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc                                                               <  1:4241725/93‑1 (MQ=255)
                            gggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGa                                                             >  1:2642652/7‑101 (MQ=16)
                            gggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGa                                                             <  2:1330376/95‑1 (MQ=12)
                             ggcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGaa                                                            <  2:3561214/96‑1 (MQ=255)
                              gcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg                                                           >  2:2845779/5‑101 (MQ=16)
                              gcgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg                                                           <  1:2348519/97‑1 (MQ=255)
                               cgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                                                          <  2:3560932/98‑1 (MQ=16)
                               cgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                                                          <  2:369306/98‑1 (MQ=16)
                               cgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                                                          >  1:311941/4‑101 (MQ=16)
                               cgctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                                                          >  1:1837180/4‑101 (MQ=16)
                                 ctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc                                                        >  1:1511444/2‑101 (MQ=16)
                                 ctGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc                                                        >  2:1163031/2‑101 (MQ=16)
                                  tGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                                                       >  2:3732371/1‑101 (MQ=19)
                                  tGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                                                       >  1:60797/1‑101 (MQ=19)
                                  tGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                                                       >  2:132586/1‑101 (MQ=255)
                                  tGCCCCTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                                                       <  1:1792210/101‑1 (MQ=16)
                                    ccccTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                                     <  2:3371320/101‑1 (MQ=255)
                                    ccccTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                                     <  2:2569006/101‑1 (MQ=255)
                                    ccccTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                                     <  1:4247761/101‑1 (MQ=255)
                                     cccTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGc                                                    <  2:628199/101‑1 (MQ=16)
                                       cTCCTGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGCGCCAAATACGCCTCTGGCCCGAAGGGCaaccgc                                                  >  2:1734377/1‑97 (MQ=12)
                                        tcctGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcgc                                                 >  2:3470728/4‑101 (MQ=255)
                                         cctGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcgcg                                                >  1:4355323/3‑101 (MQ=19)
                                         cctGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcgag                                                >  2:2059394/3‑99 (MQ=19)
                                          ctGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCgcgc                                               >  2:1777345/2‑101 (MQ=19)
                                          ctGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCgagc                                               >  2:549434/2‑101 (MQ=16)
                                           tGGCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGcc                                              >  1:2792553/1‑101 (MQ=255)
                                            ggCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                                             >  2:3266173/1‑101 (MQ=255)
                                            ggCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                                             >  1:4136000/1‑101 (MQ=255)
                                             gCCTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGa                                            <  1:2173129/101‑1 (MQ=21)
                                              ccTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa                                           <  1:255591/101‑1 (MQ=19)
                                              ccTTTCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa                                           <  1:235663/101‑1 (MQ=19)
                                                 ttCGGCCAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgc                                        <  2:3417890/101‑1 (MQ=25)
                                                 ttCGGCCAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgc                                        <  1:1594419/101‑1 (MQ=25)
                                                 ttCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgc                                        <  1:4156747/101‑1 (MQ=21)
                                                 ttCGGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgc                                        <  2:3656425/101‑1 (MQ=19)
                                                  tcggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcg                                       <  1:761875/96‑1 (MQ=19)
                                                   cggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcgc                                      >  1:3464967/5‑101 (MQ=255)
                                                    ggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGcgcg                                     >  2:3793398/4‑101 (MQ=255)
                                                       cAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcc                                  >  1:1977741/1‑101 (MQ=255)
                                                         attcaccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcccc                                >  1:3922103/7‑101 (MQ=255)
                                                          ttcaccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccccc                               <  2:3515115/96‑1 (MQ=25)
                                                           tcaccccGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATCCGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcccccc                              <  1:1104200/97‑1 (MQ=255)
                                                           tcaccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcccccc                              <  1:3490927/97‑1 (MQ=25)
                                                           tcaccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcccccc                              >  1:455551/5‑101 (MQ=255)
                                                            ccccccGGGAGGTTTGGGCC..AGGGTG.AACC.CCCTTTTTTTTTCTTGGCCCAAATCCGCCCCTGCCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCt                             <  1:194938/98‑1 (MQ=255)
                                                            caccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCt                             >  2:1572447/4‑101 (MQ=255)
                                                             accccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTg                            >  1:2293115/3‑101 (MQ=255)
                                                               cccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGAt                          >  1:2230993/1‑101 (MQ=255)
                                                                ccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATc                         >  2:4087160/1‑101 (MQ=255)
                                                                ccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATc                         >  2:2190759/1‑101 (MQ=255)
                                                                ccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAAGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATc                         <  1:3623009/101‑1 (MQ=255)
                                                                 cGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCg                        >  2:3938572/1‑101 (MQ=255)
                                                                 cGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCg                        >  1:2412738/1‑101 (MQ=255)
                                                                  gggATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGt                       <  2:1660085/101‑1 (MQ=37)
                                                                   ggatgtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTg                      >  1:1091621/7‑101 (MQ=255)
                                                                     atgtttGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGcc                    <  1:3266405/97‑1 (MQ=37)
                                                                      tgtttGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCt                   <  1:4292402/98‑1 (MQ=37)
                                                                      tgtttGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCGGGCCCGAGGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCt                   <  2:1091784/98‑1 (MQ=25)
                                                                      tgtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCt                   >  1:4334890/4‑101 (MQ=255)
                                                                      tgtttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCt                   >  2:4057694/4‑101 (MQ=255)
                                                                        tttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCa                 >  1:2836101/2‑101 (MQ=255)
                                                                        tttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCa                 >  1:279279/2‑101 (MQ=255)
                                                                        tttGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCa                 >  2:3960395/2‑101 (MQ=255)
                                                                        tttGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCa                 >  1:165109/2‑101 (MQ=255)
                                                                         ttGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAg                <  2:204152/101‑1 (MQ=38)
                                                                            tggtccaaggTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATGCGCCTCTGCCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGt               <  2:1963303/93‑1 (MQ=255)
                                                                             gggccaaggTGAAAC..CCCTTGTTTTTTCTTGGCGCAAATACGCCTCTGGCCGGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTc              <  2:490526/94‑1 (MQ=255)
                                                                             gggccaaggTGAAAC..CCCTTGCTTTTTCTTGGCGAAAATCCGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTc              <  2:1991471/94‑1 (MQ=255)
                                                                                caaaggTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATCCGCCTCGGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTCGAg           <  2:1896349/97‑1 (MQ=255)
                                                                                 caaggTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTCGAgc          >  1:1439518/4‑101 (MQ=255)
                                                                                   aggTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTCGAgcgc        >  1:3389947/2‑101 (MQ=255)
                                                                                        tgaacccccTTGCTTTTTCTTGGCGAAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTCGAGCGCCtt     <  2:4408392/95‑1 (MQ=255)
                                                                                           aaccccTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTCGAGCGCCTTGaa  <  2:165088/98‑1 (MQ=255)
                                                                                              |                                                                                                 
GCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCGG.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTCAGTCGAGCGCCTTGAA  >  NC_014034/1696124‑1696314

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG