Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,786,540 Δ1 bp 95.5% coding (674/819 nt) RCAP_rcc01648 → ABC transporter permease

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,786,5400C→.95.5% 17.3 ‑1.4 ‑1.0192coding (674/819 nt)RCAP_rcc01648ABC transporter permease
Reads supporting (aligned to +/- strand):  new base (80/102):  ref base (1/6):  total (82/110)
Fisher's exact test strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.95e-02

Only 100 of 198 total aligned reads displayed.

GCTGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGCGTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCT.GGCCGCTCGAACGGGATCGGCCTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCT  >  NC_014034/1786442‑1786636
                                                                                                   |                                                                                                
  tGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGGCTtt                                                                                               <  2:2853733/101‑1 (MQ=255)
  tGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGGCTtt                                                                                               >  1:3816309/1‑101 (MQ=255)
  tGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGGCTtt                                                                                               <  2:453008/101‑1 (MQ=255)
  tGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGGCTtt                                                                                               <  1:1812768/101‑1 (MQ=255)
  tGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGGCTtt                                                                                               <  1:2062174/101‑1 (MQ=255)
   gATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGGCTttc                                                                                              <  1:2713295/101‑2 (MQ=255)
   gATGCCGCAGCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGGCTttc                                                                                              <  2:243077/101‑2 (MQ=255)
     tGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGt                                                                                           <  1:761709/101‑1 (MQ=255)
     tGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGt                                                                                           <  1:1424753/101‑1 (MQ=255)
     tGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGt                                                                                           <  1:2407780/101‑1 (MQ=255)
     tGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGt                                                                                           <  2:2216256/101‑1 (MQ=255)
     tGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGt                                                                                           <  2:2391232/101‑1 (MQ=255)
      gCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTa                                                                                          <  2:3809547/101‑1 (MQ=255)
      gCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTa                                                                                          <  2:584833/101‑1 (MQ=255)
        cGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATc                                                                                        <  1:507803/101‑1 (MQ=255)
         gCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATcc                                                                                       >  2:556155/1‑101 (MQ=255)
           aTCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCAt                                                                                     <  1:1157673/101‑1 (MQ=255)
               gATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGa                                                                                 <  1:2041936/101‑1 (MQ=255)
               gATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGa                                                                                 <  2:2365316/101‑1 (MQ=255)
               gATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGa                                                                                 <  1:3625090/101‑1 (MQ=255)
               gATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGa                                                                                 >  2:2022552/1‑101 (MQ=255)
               gATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGa                                                                                 >  2:2492948/1‑101 (MQ=255)
                aTCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGaa                                                                                <  1:2534956/101‑1 (MQ=255)
                  cgccgcGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAAtt                                                                              <  1:2447438/101‑1 (MQ=255)
                   gccgcGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAAttt                                                                             >  1:1235985/1‑101 (MQ=255)
                     cgcggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTcc                                                                           >  1:2863114/1‑101 (MQ=255)
                      gcggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCa                                                                          <  2:523636/101‑1 (MQ=255)
                       cggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAg                                                                         <  1:1533055/101‑1 (MQ=255)
                       cggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAg                                                                         >  1:3782554/1‑101 (MQ=255)
                        ggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGa                                                                        >  2:675244/1‑101 (MQ=255)
                         gcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGAt                                                                       <  2:1235892/101‑1 (MQ=255)
                            cgggcgggGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGtt                                                                    <  2:4196906/101‑1 (MQ=255)
                             gggcggggCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTc                                                                   <  1:3551369/101‑1 (MQ=255)
                             gggcggggCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTc                                                                   <  1:3676474/101‑1 (MQ=255)
                              ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                  <  2:1166927/101‑1 (MQ=255)
                              ggcggggcTCTCGCCGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                  <  2:1773827/101‑1 (MQ=255)
                                cggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGAc                                                                <  1:2918364/95‑1 (MQ=255)
                                cggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGAc                                                                <  1:1486997/95‑1 (MQ=255)
                                  gggctttCGCTGATCTGGTAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATC.GTCTTTCGTATCCATCTGAATTTCCAGATGTTCGACAt                                                              <  2:1742060/97‑1 (MQ=255)
                                   ggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAtt                                                             >  1:1987416/4‑101 (MQ=255)
                                    gctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTg                                                            <  2:776056/99‑1 (MQ=255)
                                     ctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGc                                                           <  1:2946949/100‑1 (MQ=255)
                                      tttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGcc                                                          <  1:3020473/101‑1 (MQ=255)
                                       ttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCg                                                         >  2:638158/1‑101 (MQ=255)
                                         cGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAg                                                       <  2:955822/101‑1 (MQ=255)
                                            tGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTg                                                    >  1:1668115/1‑101 (MQ=255)
                                            tGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTg                                                    >  2:3290579/1‑101 (MQ=255)
                                            tGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTg                                                    <  2:4404758/101‑1 (MQ=255)
                                            tGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTg                                                    <  2:1442494/101‑1 (MQ=255)
                                             gATCTGGAAGGCCGGGCTGGGGGGCGAATTTATAGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGTATTTCCAGATGTTCGACATAGCCGAGGTGc                                                   >  2:1892743/1‑101 (MQ=255)
                                               tCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTc                                                 <  2:1245672/101‑1 (MQ=255)
                                                cTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCg                                                >  2:2572809/1‑101 (MQ=255)
                                                    aaGGTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTg                                            <  2:1218740/101‑1 (MQ=255)
                                                      ggTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTggg                                          <  1:4383302/101‑1 (MQ=255)
                                                       gTCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggc                                         >  2:3036154/1‑101 (MQ=255)
                                                        tCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcg                                        <  2:2316079/101‑1 (MQ=255)
                                                        tCGTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcg                                        <  2:3414538/101‑1 (MQ=255)
                                                          gTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggc                                      >  1:1928432/1‑101 (MQ=255)
                                                          gTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggc                                      >  2:1495862/1‑101 (MQ=255)
                                                          gTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggc                                      >  2:3723684/1‑101 (MQ=255)
                                                          gTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggc                                      <  2:433188/101‑1 (MQ=255)
                                                          gTGCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggc                                      >  1:1061604/1‑101 (MQ=255)
                                                            gCTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGGGCTCGCCTGggcggcgg                                    >  2:2451598/1‑101 (MQ=255)
                                                             cTGGTGGTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggcggc                                   <  2:1558443/101‑1 (MQ=255)
                                                              tggtggTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTTCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCt                                  >  2:2777854/1‑101 (MQ=255)
                                                              tggtggTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCt                                  >  1:2335699/1‑101 (MQ=255)
                                                              tggtggTCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCt                                  >  1:3138874/1‑101 (MQ=255)
                                                                gtggtCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGCGGTGCTCGCCTGGGCGGCGGCttt                                >  2:3868122/1‑101 (MQ=255)
                                                                gtggtCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCttt                                >  1:3596735/1‑101 (MQ=255)
                                                                  ggtCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTGGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCg                              >  2:1406067/1‑101 (MQ=255)
                                                                    tCGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTc                            <  2:1611086/101‑1 (MQ=255)
                                                                     cGAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCt                           >  2:4066044/1‑101 (MQ=255)
                                                                      gAATTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGGGGCGGCTTTCGTCTg                          >  1:2372107/1‑101 (MQ=255)
                                                                       aaTTTCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGc                         <  2:4249625/101‑1 (MQ=255)
                                                                         tttCTGGGCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGt                       <  1:3270679/101‑1 (MQ=255)
                                                                            ctgggCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGAt                    >  1:3050790/4‑101 (MQ=255)
                                                                             tgggCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATg                   <  2:1503801/99‑1 (MQ=255)
                                                                             tgggCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATg                   <  1:1743672/99‑1 (MQ=255)
                                                                             tgggCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATg                   >  1:1034408/3‑101 (MQ=255)
                                                                              gggCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGc                  >  2:3452382/2‑101 (MQ=255)
                                                                              gggCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGGGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGc                  >  2:724370/2‑101 (MQ=255)
                                                                               ggCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCt                 <  1:852674/101‑1 (MQ=255)
                                                                                gCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTc                <  2:870572/101‑1 (MQ=255)
                                                                                gCCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTc                <  1:3235688/101‑1 (MQ=255)
                                                                                  cGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGc              >  1:155301/1‑101 (MQ=255)
                                                                                  cGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGCCTGCGGGATGCTCGc              >  2:3064035/1‑101 (MQ=255)
                                                                                   gCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGcc             <  1:207317/101‑1 (MQ=255)
                                                                                    cTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCg            <  2:3596487/101‑1 (MQ=255)
                                                                                     tCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGt           <  2:326165/101‑1 (MQ=255)
                                                                                      cGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTc          <  1:582609/101‑1 (MQ=255)
                                                                                       gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg         >  2:2923103/1‑101 (MQ=255)
                                                                                       gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg         <  1:436699/101‑1 (MQ=255)
                                                                                       gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg         <  1:879048/101‑1 (MQ=255)
                                                                                         aCGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGaa       >  1:724089/1‑101 (MQ=255)
                                                                                          cGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAAt      <  2:1061516/101‑1 (MQ=255)
                                                                                           gggATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATg     >  1:3373828/1‑101 (MQ=255)
                                                                                           gggATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATg     >  1:645940/1‑101 (MQ=255)
                                                                                           gggATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATg     <  2:3368538/101‑1 (MQ=255)
                                                                                             gATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGc   >  2:3173172/1‑101 (MQ=255)
                                                                                              aTCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCt  >  1:3960083/1‑101 (MQ=255)
                                                                                                   |                                                                                                
GCTGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGCGTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCT.GGCCGCTCGAACGGGATCGGCCTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCT  >  NC_014034/1786442‑1786636

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG