Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,843,359	Δ1 bp	100%	intergenic (+21/+127)	ppiB → / ← tyrS	peptidyl‑prolyl cis‑trans isomerase B/tyrosyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,843,359	0	C→.	100.0%	222.0	63	intergenic (+21/+127)	ppiB/tyrS	peptidyl‑prolyl cis‑trans isomerase B/tyrosyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  new base (14/49):  ref base (0/0):  total (14/49)

TGAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCA.GGGGGCTCTGCCCCCCCTCTG.TGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCG.TCCGCT.GGGGCGCCCTTTCATCTTGCCACAAA  >  NC_014034/1843265‑1843452                                                                                                |                                                                                                 tGAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTGcccccc                                                                                             <  2:566834/101‑1 (MQ=255)  gAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTGcccccct                                                                                            <  2:301610/101‑2 (MQ=255)   aaaacGGGCGCGCCCGAGCTGACCGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTGcccccctc                                                                                           <  2:606434/101‑3 (MQ=255)   aaaaaGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTGcccccctc                                                                                           <  1:1427419/101‑3 (MQ=255)    aaaaGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGGTCTGcccccctct                                                                                          >  1:925673/1‑98 (MQ=255)    aaaaGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTGcccccctct                                                                                          <  2:3112867/101‑4 (MQ=255)    aaaaGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTGcccccctct                                                                                          <  2:1089535/101‑4 (MQ=255)     aaaGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTGcccccctctg                                                                                         <  1:767935/101‑5 (MQ=255)     aaaGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTGcccccctctg                                                                                         <  1:4017287/101‑5 (MQ=255)       aGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTGcccccctctgct                                                                                       <  2:628839/101‑7 (MQ=255)       aGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTGcccccctctgct                                                                                       <  2:2423551/101‑7 (MQ=255)        gggCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTGcccccctctgctg                                                                                      <  1:3145780/101‑8 (MQ=255)        gggCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTGcccccctctgctg                                                                                      <  1:3557094/101‑8 (MQ=255)        gggCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTGcccccctctgctg                                                                                      <  1:1366920/101‑8 (MQ=255)          gcgcgcCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTGcccccctctgctgcg                                                                                    <  2:1318262/101‑10 (MQ=255)           cgcgcCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCACGGGGGGGGTGcccccccccccccccc                                                                                   >  1:1364117/1‑92 (MQ=255)            gcgcCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACTATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCa                                                                                 <  2:3352812/101‑1 (MQ=255)             cgcCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCAt                                                                                >  1:463207/1‑101 (MQ=255)             cgcCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCGCTG.CCCCCCTCTGCTGCGCAt                                                                                <  2:4162291/101‑1 (MQ=255)             cgcCCGAGCTGAACGGCCTGGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCAt                                                                                <  2:463167/101‑1 (MQ=255)              gcCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCAtt                                                                               <  2:1848721/101‑1 (MQ=255)               cccGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTc                                                                              <  2:74121/101‑1 (MQ=255)                  gAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCAcc                                                                           <  1:2141530/101‑1 (MQ=255)                    gCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCAcccc                                                                         <  2:4297086/101‑1 (MQ=255)                     cTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCAccccc                                                                        <  1:4021658/101‑1 (MQ=255)                      tGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCAcccccc                                                                       <  1:3266719/101‑1 (MQ=255)                        aaCGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCgg                                                                     <  2:2845408/101‑1 (MQ=255)                         aCGGCCTTGTCGAAGCCCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCggg                                                                    <  2:932457/101‑1 (MQ=38)                         aCGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCggg                                                                    <  2:114396/101‑1 (MQ=38)                          cGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGa                                                                   <  1:1712312/101‑1 (MQ=38)                           ggCCTTGTCGAAGGCCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCCCCCCCCGGGat                                                                  <  1:1484727/101‑1 (MQ=255)                           ggCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGat                                                                  <  1:2192/101‑1 (MQ=38)                              cTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATAtt                                                               <  1:2650012/101‑1 (MQ=37)                               ttGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATAttt                                                              <  2:2483763/101‑1 (MQ=37)                                tGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATAtttt                                                             <  1:112660/101‑1 (MQ=37)                                  tCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCg                                                           <  1:2124678/101‑1 (MQ=37)                                      aGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTCCGCATTCACCCCCCGGGATATTTTCGCCaa                                                       >  2:1764752/1‑101 (MQ=37)                                       gcccccGATTACAGCGGCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAg                                                      <  1:7402/99‑1 (MQ=25)                                       gACCCCGATTACATCGGCAAGGCCCCGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCACCCGGGATATTTTGGCCAAg                                                      <  2:135358/101‑1 (MQ=25)                                         ccccGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGt                                                    <  2:3851949/101‑1 (MQ=37)                                            cGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGaa                                                 <  1:3399906/101‑1 (MQ=25)                                               ttACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATg                                              <  1:3899108/101‑1 (MQ=25)                                               ttACATCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACACCCCGGGATATTTTCGCCAAGGTGAAATg                                              <  2:3349813/101‑1 (MQ=37)                                                   aTCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGaaa                                          <  2:1012221/101‑1 (MQ=25)                                                   aTCGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGaaa                                          <  2:1724556/101‑1 (MQ=25)                                                    tCGTCAAGGCCACGATCGAGTGACCCGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGaaaa                                         <  1:619187/101‑1 (MQ=25)                                                     cGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAg                                        <  2:2936810/101‑1 (MQ=25)                                                     cGTCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAg                                        <  2:3358094/101‑1 (MQ=25)                                                       tCAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAggg                                      <  1:3669318/101‑1 (MQ=25)                                                        cAAGGCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGc                                     <  2:3653706/101‑1 (MQ=25)                                                           ggCCACGATCGAGTGACACGGGCGGAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCCCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgct                                  <  1:1344912/101‑3 (MQ=25)                                                            gCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgctc                                 <  1:2260504/101‑4 (MQ=25)                                                            gCCACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgctc                                 <  2:2586165/101‑4 (MQ=25)                                                             ccACGATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCgctcc                                <  1:2325536/101‑5 (MQ=25)                                                                 gATCGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.g                           <  2:1362429/101‑1 (MQ=25)                                                                    cGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gggg                        <  1:64517/101‑1 (MQ=25)                                                                    cGAGTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gggg                        <  2:3584193/101‑1 (MQ=25)                                                                      ggTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCAGTCGCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gggggc                      <  2:2653902/100‑3 (MQ=21)                                                                       gTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gggggcg                     <  2:2226270/101‑4 (MQ=21)                                                                       gTGACACGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCT.gggggcg                     <  2:1198099/101‑4 (MQ=21)                                                                          acacGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGccc                   <  2:469918/101‑1 (MQ=21)                                                                             cGGGCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCttt                <  1:855321/101‑1 (MQ=21)                                                                              gggCGCAGGGGGGCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTc               <  1:1244772/101‑1 (MQ=21)                                                                                 cgcaggggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATc            <  2:194932/96‑1 (MQ=21)                                                                                  gcaggggggCTCTG.CCCCCCTCTGCTGCGCATTCTCCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCt           <  1:1409978/97‑1 (MQ=21)                                                                                   caggggggCTC.GGCCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCtt          <  1:4374786/98‑1 (MQ=21)                                                                                    aggggggCTCTG.CCCCGCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTg         <  2:232333/99‑1 (MQ=21)                                                                                     ggggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGc        >  1:2054684/2‑101 (MQ=21)                                                                                     ggggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGc        >  2:1613570/2‑101 (MQ=21)                                                                                       ggggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCca      >  1:3165235/1‑101 (MQ=25)                                                                                        gggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCcac     <  2:535051/101‑1 (MQ=25)                                                                                        gggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCcac     >  2:1488745/1‑101 (MQ=25)                                                                                         ggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCcaca    <  2:971350/101‑1 (MQ=25)                                                                                         ggCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCcaca    >  1:4231826/1‑101 (MQ=25)                                                                                          gCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACaa   >  2:3790754/1‑101 (MQ=25)                                                                                          gCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACaa   >  2:2060947/1‑101 (MQ=25)                                                                                          gCTCTG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACaa   >  1:4291869/1‑101 (MQ=25)                                                                                           ctctG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCTAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACaaa  <  2:525247/101‑1 (MQ=25)                                                                                           ctctG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACaaa  >  1:470121/1‑101 (MQ=25)                                                                                           ctctG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACaaa  <  2:2377166/101‑1 (MQ=25)                                                                                           ctctG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACaaa  >  2:1137743/1‑101 (MQ=25)                                                                                           ctctG.CCCCCCTCTGCTGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACaaa  >  2:2803854/1‑101 (MQ=25)                                                                                           ctctG.CCCCCCTCTGCTGCGCATTCACCCCCCAGGTTATTTTCGCCAAGGTGCAATGGAACAGGGCGCTCCGCTGGGGGCGCCCTTTCATCTTGCCACaaa  >  2:1469146/1‑101 (MQ=255)                                                                                                |                                                                                                 TGAAAAAGGGCGCGCCCGAGCTGAACGGCCTTGTCGAAGACCCCGATTACATCGTCAAGGCCACGATCGAGTGACACGGGCGCA.GGGGGCTCTGCCCCCCCTCTG.TGCGCATTCACCCCCCGGGATATTTTCGCCAAGGTGAAATGGAAAAGGGCG.TCCGCT.GGGGCGCCCTTTCATCTTGCCACAAA  >  NC_014034/1843265‑1843452 Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG