Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,027,931 Δ1 bp 100% intergenic (‑337/‑16) RCAP_rcc01873 ← / → RCAP_rcc01874 peptidoglycan‑binding domain 1 protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,027,9310T→.100.0% 816.3 182intergenic (‑337/‑16)RCAP_rcc01873/RCAP_rcc01874peptidoglycan‑binding domain 1 protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (87/95):  ref base (0/0):  total (87/95)

Only 100 of 198 total aligned reads displayed.

TAAGTCCGGATCAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCCTTTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCCACATGTATTCC  >  NC_014034/2027831‑2028027
                                                                                                    |                                                                                                
gaaGCCCGGAACAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCCt                                                                                                  <  1:1037064/100‑1 (MQ=255)
 aaGTCCGGATCAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCCtt                                                                                                 >  1:3121313/1‑101 (MQ=255)
 aaGTCCGGATCAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCCtt                                                                                                 >  2:634181/1‑101 (MQ=255)
 aaGCCCGGATCAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCCtt                                                                                                 <  1:4235619/101‑1 (MQ=255)
  agccgggaaaaaGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCCttt                                                                                                <  1:386688/91‑1 (MQ=255)
  aGTCCGGATCAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCCttt                                                                                                >  2:1331119/1‑101 (MQ=255)
   gcccGGATCAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCCtttg                                                                                               <  2:2586847/99‑2 (MQ=255)
   gTCCGGATCAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCCtttg                                                                                               >  2:2734291/1‑100 (MQ=255)
    tCCGGATCAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCCtttgc                                                                                              >  1:2112443/1‑99 (MQ=255)
       ggATCAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTtgctgc                                                                                          >  1:221768/1‑101 (MQ=255)
        gATCAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGcc                                                                                         <  2:2854483/101‑1 (MQ=255)
          caaaGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCAc                                                                                       <  2:3727413/99‑1 (MQ=255)
          acAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCAc                                                                                       <  2:2625068/100‑1 (MQ=255)
           cAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACg                                                                                      <  1:2063831/101‑1 (MQ=255)
             aGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCt                                                                                    >  1:909483/1‑101 (MQ=255)
             aGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCt                                                                                    <  1:4198587/101‑1 (MQ=255)
             aGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCt                                                                                    <  1:296894/101‑1 (MQ=255)
              gCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCtt                                                                                   <  2:696911/101‑1 (MQ=255)
               ccGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTtg                                                                                  <  2:3897709/101‑1 (MQ=255)
                cGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTtgt                                                                                 <  2:759553/101‑1 (MQ=255)
                cGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTtgg                                                                                 >  2:1292254/1‑100 (MQ=255)
                    aaCTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTa                                                                             <  1:4427865/101‑1 (MQ=255)
                    aaCTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTa                                                                             <  1:178696/101‑1 (MQ=255)
                      cTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTagc                                                                           >  1:4264898/1‑101 (MQ=255)
                      cTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTagc                                                                           <  1:983090/101‑1 (MQ=255)
                            cGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGcccc                                                                     <  2:3436348/101‑1 (MQ=255)
                            cGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGcccc                                                                     >  1:303846/1‑101 (MQ=255)
                             ggCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCg                                                                    <  2:1230244/101‑1 (MQ=255)
                              gCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGa                                                                   >  2:1606210/1‑101 (MQ=255)
                               cacaGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGaa                                                                  >  1:3018681/1‑101 (MQ=255)
                                acaGACCGAGGGACAGGCGAACCGATATGGGCACGATAACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGgaa                                                                 >  2:2466931/1‑101 (MQ=255)
                                 caGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAg                                                                <  1:3597483/101‑1 (MQ=255)
                                  aGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGc                                                               >  2:3360628/1‑101 (MQ=255)
                                  aGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGc                                                               >  2:297033/1‑101 (MQ=255)
                                    aCCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCt                                                             >  2:2376700/1‑101 (MQ=255)
                                      cGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTcc                                                           >  1:3999816/1‑101 (MQ=255)
                                      cGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTcc                                                           <  2:2153914/101‑1 (MQ=255)
                                       gAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCa                                                          <  2:933134/101‑1 (MQ=255)
                                       gAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCa                                                          >  2:1974290/1‑101 (MQ=255)
                                         gggACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAAc                                                        >  2:2017611/1‑101 (MQ=255)
                                         gggACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAAc                                                        >  1:370209/1‑101 (MQ=255)
                                          ggACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACg                                                       >  2:2278804/1‑101 (MQ=255)
                                           gACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGc                                                      >  2:2257851/1‑101 (MQ=255)
                                             cAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCt                                                    >  1:612916/1‑101 (MQ=255)
                                             cAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCt                                                    >  1:3228247/1‑101 (MQ=255)
                                             cAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAAAGCCt                                                    >  1:2591947/1‑101 (MQ=255)
                                               ggCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTc                                                  >  2:986561/1‑101 (MQ=255)
                                                 ggAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAg                                                <  2:1279520/100‑1 (MQ=255)
                                                 cGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAg                                                <  2:498508/101‑1 (MQ=255)
                                                 cGAACCGATATGGGCACGATCACGACTTCTCGTCGCGCGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAg                                                <  1:3757290/101‑1 (MQ=255)
                                                  gAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGc                                               >  1:4118821/1‑101 (MQ=255)
                                                    aCCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCtt                                             <  2:3256702/101‑1 (MQ=255)
                                                     ccGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTc                                            <  2:909398/101‑1 (MQ=255)
                                                      cGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTcc                                           >  1:1478040/1‑101 (MQ=255)
                                                      cGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTcc                                           >  2:3549871/1‑101 (MQ=255)
                                                       gATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTccg                                          >  2:1659372/1‑100 (MQ=255)
                                                       gATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCt                                          >  1:933986/1‑101 (MQ=255)
                                                       gATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCt                                          >  1:3427717/1‑101 (MQ=255)
                                                        atatGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTg                                         <  2:1166519/101‑1 (MQ=255)
                                                         tatGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTgc                                        >  2:3037612/1‑101 (MQ=255)
                                                         tatGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTgc                                        <  1:1606316/101‑1 (MQ=255)
                                                          atGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTgcg                                       >  1:2444044/1‑101 (MQ=255)
                                                          atGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTgcg                                       >  2:1879727/1‑101 (MQ=255)
                                                           tGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTgcgc                                      <  2:3816485/101‑1 (MQ=255)
                                                            gggCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGcgcg                                     >  2:2991434/1‑101 (MQ=255)
                                                            gggCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGcgcg                                     <  1:1974419/101‑1 (MQ=255)
                                                               cACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCgcggcg                                  <  2:3509496/101‑1 (MQ=255)
                                                                 cGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCt                                <  1:1331216/101‑1 (MQ=255)
                                                                 cGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCt                                <  1:1136731/101‑1 (MQ=255)
                                                                 cGAGCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCt                                <  2:220961/101‑1 (MQ=255)
                                                                  gATCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTg                               <  1:2020033/101‑1 (MQ=255)
                                                                   aTCACGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTgg                              <  1:921315/101‑1 (MQ=255)
                                                                     cACGACTTCTCGGCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCg                            <  2:612848/101‑1 (MQ=255)
                                                                      aCGACTTCTCGTCGCGGGCTTCTTGTGGCC.TTTGCTGCCACGCTTGTGGTAGTAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGa                           >  1:1764287/1‑101 (MQ=255)
                                                                       cGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGat                          <  2:2544313/101‑1 (MQ=255)
                                                                       cGACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGat                          <  2:3016709/101‑1 (MQ=255)
                                                                        gACTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTgacccca                         <  2:2561391/101‑7 (MQ=255)
                                                                         aCTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGatat                        >  1:1836382/1‑101 (MQ=255)
                                                                          cTTCTCGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATc                       <  1:3073252/101‑1 (MQ=255)
                                                                            tctcGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCg                     >  2:2820944/1‑101 (MQ=255)
                                                                            tctcGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCg                     <  1:1703293/101‑1 (MQ=255)
                                                                             ctcGTCGCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGt                    >  1:354810/1‑101 (MQ=255)
                                                                              tcgtcgCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTc                   <  2:4440593/101‑1 (MQ=255)
                                                                              tcgtcgCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTc                   <  2:1141230/101‑1 (MQ=255)
                                                                               gccgcggggCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCg                  <  1:796486/95‑1 (MQ=255)
                                                                               cgtcgCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCg                  <  2:4380866/101‑1 (MQ=255)
                                                                                gtcgCGGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCgg                 <  2:1750815/101‑1 (MQ=255)
                                                                                  cgcgGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGAt               >  2:1366581/1‑101 (MQ=255)
                                                                                   gcgGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATc              <  1:1491745/101‑1 (MQ=255)
                                                                                    cgGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATcc             <  1:583292/101‑1 (MQ=255)
                                                                                    cgGGCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATcc             >  2:4321256/1‑101 (MQ=255)
                                                                                     gggCTTCTTGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCca            <  1:2280909/101‑1 (MQ=255)
                                                                                         ttcttGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCCACATg        >  2:4217748/1‑101 (MQ=255)
                                                                                         ttcttGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCCACATg        <  2:912472/101‑1 (MQ=255)
                                                                                         ttcttGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCCACATg        >  1:1168053/1‑101 (MQ=255)
                                                                                           cttGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCCACATGTa      >  2:3163177/1‑101 (MQ=255)
                                                                                           cttGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCCACATGTa      >  1:3058534/1‑101 (MQ=255)
                                                                                            ttGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCCACATGTAt     >  1:429822/1‑101 (MQ=255)
                                                                                            ttGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCCACATGTAt     <  2:2067425/101‑1 (MQ=255)
                                                                                             tGCGGCC.TTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCCACATGTAtt    <  1:511872/101‑1 (MQ=255)
                                                                                                    |                                                                                                
TAAGTCCGGATCAAGCCGCAAACTCAGACGGCACAGACCGAGGGACAGGCGAACCGATATGGGCACGATCACGACTTCTCGTCGCGGGCTTCTTGCGGCCTTTTGCTGCCACGCTTGTGGTAGCAGCCCCGAAAGCCTCCAACGCCTTCAGCTTCCTGCGCGGCGCTGGCGATATCCGTCGGATCCACATGTATTCC  >  NC_014034/2027831‑2028027

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG