Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,044,270 Δ1 bp 100% intergenic (+143/+34) typA → / ← potG1 GTP‑binding protein TypA/BipA/polyamine ABC transporter ATP‑binding protein PotG

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,044,2700A→.100.0% 49.9 147intergenic (+143/+34)typA/potG1GTP‑binding protein TypA/BipA/polyamine ABC transporter ATP‑binding protein PotG
Reads supporting (aligned to +/- strand):  new base (109/24):  ref base (6/7):  total (116/31)
Fisher's exact test strand distribution p-value = 6.91e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.72e-01

Only 100 of 185 total aligned reads displayed.

CGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAAGGGGGGCGCGAAGCCCCCCTTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGC  >  NC_014034/2044170‑2044367
                                                                                                    |                                                                                                 
cGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCa                                                                                                   >  2:1797292/1‑101 (MQ=255)
cGAATGAAATTCACCTCCCCCAGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCa                                                                                                   >  1:1495847/1‑101 (MQ=255)
 gAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaa                                                                                                  >  1:2310827/1‑101 (MQ=255)
  aaTGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaa                                                                                                 <  2:2323911/101‑1 (MQ=255)
  aaTGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaa                                                                                                 <  2:1836834/101‑1 (MQ=255)
  aaTGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaa                                                                                                 <  1:1983198/101‑1 (MQ=255)
  aaTGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaa                                                                                                 <  1:60522/101‑1 (MQ=255)
  aaTGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaa                                                                                                 <  1:686879/101‑1 (MQ=255)
   aTGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaa                                                                                                >  2:3757103/1‑101 (MQ=255)
   aTGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaa                                                                                                <  1:936252/101‑1 (MQ=255)
    tGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaaa                                                                                               <  2:2641877/101‑1 (MQ=255)
    tGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaaa                                                                                               >  2:2863169/1‑101 (MQ=255)
    tGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaaa                                                                                               <  1:1162275/101‑1 (MQ=255)
     gAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaaag                                                                                              >  2:4139128/1‑100 (MQ=255)
       tcttCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAGgg                                                                                            <  1:501994/99‑1 (MQ=255)
       aaTTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTACTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAGgg                                                                                            >  1:1660376/1‑101 (MQ=255)
       aaTTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGATGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAGgg                                                                                            >  2:3857044/1‑101 (MQ=255)
         ttCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAGgggg                                                                                          <  1:3979045/101‑1 (MQ=255)
          tCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAGggggg                                                                                         <  1:2573304/101‑1 (MQ=255)
          tCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAGggaga                                                                                         >  2:501942/1‑98 (MQ=255)
           cACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAGgggggc                                                                                        <  1:234892/101‑2 (MQ=255)
           cACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAGgggggc                                                                                        >  2:1748696/1‑100 (MQ=255)
             ccTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGgcgc                                                                                     >  2:711265/1‑101 (MQ=255)
             ccTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGgcgc                                                                                     >  1:176365/1‑101 (MQ=255)
              cTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGcgcg                                                                                    <  2:1681793/101‑1 (MQ=255)
               tCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGNGGAATTTGCACAGC.AAAAAGGGGGGCGCGa                                                                                   >  1:1068216/1‑101 (MQ=255)
               cccccccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGa                                                                                   <  1:2260379/100‑1 (MQ=255)
               cccccccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCAGCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGa                                                                                   <  1:2695927/100‑1 (MQ=255)
                 cccccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAg                                                                                 <  2:241444/101‑1 (MQ=255)
                    ccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGccc                                                                              <  1:24940/101‑1 (MQ=255)
                      gggATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGccccc                                                                            >  1:1947698/1‑101 (MQ=255)
                       ggATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGcccccc                                                                           >  1:2951674/1‑101 (MQ=255)
                         atatTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCtt                                                                         >  1:1230880/1‑101 (MQ=255)
                         atatTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCtt                                                                         <  2:1406966/101‑1 (MQ=255)
                          tatTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCttt                                                                        <  2:1280208/101‑1 (MQ=255)
                          tatTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCttt                                                                        >  1:1344615/1‑101 (MQ=255)
                          tatTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTACTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCttt                                                                        <  2:1660265/101‑1 (MQ=255)
                            tttGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCttttt                                                                      >  1:1636083/1‑101 (MQ=255)
                             ttGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCtttttc                                                                     >  2:458148/1‑100 (MQ=255)
                              tGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCtttttcc                                                                    >  2:441049/1‑99 (MQ=255)
                              tGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAG.TAAAAAGGGGGGCGCGAAGCCCCCCtttttac                                                                    >  1:1048345/1‑99 (MQ=255)
                                aGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCtttttccgt                                                                  >  2:4284578/1‑97 (MQ=255)
                                   cAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAg                                                              >  1:3290006/1‑101 (MQ=255)
                                    aaGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGc                                                             >  1:2149779/1‑101 (MQ=255)
                                     aGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCg                                                            >  2:2021863/1‑101 (MQ=255)
                                        tAAAGACTTGCGAGGTTTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAG.AAAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGaaa                                                         <  1:2045891/101‑1 (MQ=255)
                                        tAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGaaa                                                         <  2:2193991/101‑1 (MQ=255)
                                         aaggccTTGGGGGGTTTTTTTCTTTTTTCTGAAAAGCCCGGGGGGGGATTTTGCA.AAAAAAAAGGGGGGGCGCG.ACCCCCCCCTTTTTCCGTCAGCGAAAc                                                        <  1:1458620/96‑1 (MQ=255)
                                         aaaGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAAc                                                        >  1:4272219/1‑101 (MQ=255)
                                         aaaGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAAc                                                        >  1:667943/1‑101 (MQ=255)
                                         aaaGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAAc                                                        >  2:4088134/1‑101 (MQ=255)
                                         aaaGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAAc                                                        >  2:1014772/1‑101 (MQ=255)
                                         aaaGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTCTCCGTCAGCGAAAc                                                        >  2:2968506/1‑101 (MQ=255)
                                          aaGACTTGCGAGGGGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACa                                                       >  1:3587637/1‑101 (MQ=255)
                                           aGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCACCGAAACAg                                                      >  2:886865/1‑101 (MQ=255)
                                             aCTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCa                                                    >  2:3350717/1‑101 (MQ=255)
                                                  cGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgc                                               >  2:1322554/1‑101 (MQ=255)
                                                  cGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgc                                               >  2:2356433/1‑101 (MQ=255)
                                                   gAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgcg                                              >  2:3034565/1‑101 (MQ=255)
                                                      gtgtTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgcgcgc                                           >  1:1418958/1‑101 (MQ=255)
                                                       tgtTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGcc                                          >  2:4016184/1‑101 (MQ=255)
                                                        gtTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCg                                         >  2:845958/1‑101 (MQ=255)
                                                           cttcATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGc                                      >  2:2682679/1‑101 (MQ=255)
                                                            ttcATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCg                                     >  1:3357889/1‑101 (MQ=255)
                                                             tcATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGa                                    >  2:3336522/1‑101 (MQ=255)
                                                              cATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGAc                                   >  1:1546022/1‑101 (MQ=255)
                                                                tCTTTCTGCAAAGCCCGGGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCa                                 <  2:2012795/101‑1 (MQ=255)
                                                                tCTTTCGGAAAGGACCGGGGGGGAAATTTGC.CAAGAAAAAGGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCa                                 <  2:1191337/101‑1 (MQ=255)
                                                                  tttCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTTAGCGAAACAGCACCAGCGTGCCGGGCGACCAgc                               >  1:3844979/1‑100 (MQ=255)
                                                                  tttCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCCGCGCGCCGGGCGACCAgg                               >  1:848285/1‑101 (MQ=255)
                                                                     cTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCa                            >  1:2234396/1‑101 (MQ=255)
                                                                     cTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCa                            >  2:2917881/1‑101 (MQ=255)
                                                                     cTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCa                            >  1:1959442/1‑101 (MQ=255)
                                                                     cTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCa                            >  1:1271291/1‑101 (MQ=255)
                                                                      tGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCAc                           >  1:1123271/1‑101 (MQ=255)
                                                                      tGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCAc                           <  2:564954/101‑1 (MQ=255)
                                                                       gCAAAGACCGGGGGGGGATTTTGCACAG.AAAAAGGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCAcc                          <  2:419171/101‑1 (MQ=255)
                                                                       gCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGGCAcc                          >  1:4114655/1‑101 (MQ=255)
                                                                        aaaaGGCCGGGGGGGGAATTTGCACAG.AAAAAGGGGGGGCGCGACGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCAccc                         <  1:2783458/100‑1 (MQ=255)
                                                                         aaaGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCcg                        >  2:2254374/1‑101 (MQ=255)
                                                                         aaaGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCcg                        >  1:873961/1‑101 (MQ=255)
                                                                           aGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGGCAGCGAAACAGCACCACCGCGCCGGCCGACCAGGCAACCcccg                      >  2:3983954/1‑101 (MQ=255)
                                                                            gccgggggggggAATTTCCACA.AAAAAAGGGGGGGCG.AAACCCCCCCCTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGt                     <  1:631785/95‑1 (MQ=255)
                                                                             gccGTGGGGGGAATTTGCCCAG.AAAAAAGGGGGGTGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTc                    <  1:4181093/100‑1 (MQ=255)
                                                                             ccggggggggaAATTCGCA.AGCAAAAAGGGGGGCCGC.AAACCCCCCCTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTc                    <  1:2863377/96‑1 (MQ=255)
                                                                                 cgGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTggg                >  2:1636089/1‑101 (MQ=255)
                                                                                  ggggggAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGt               >  1:3174031/1‑101 (MQ=255)
                                                                                  ggggggAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGt               >  1:4194335/1‑101 (MQ=255)
                                                                                  ggggggAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGt               >  2:1476271/1‑101 (MQ=255)
                                                                                   gggggAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTg              >  2:2595629/1‑101 (MQ=255)
                                                                                      ggAATTTGCACAGC.AAAAAGGGGGGCGCGAA.CCCCCCCTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCg           <  1:650139/101‑1 (MQ=255)
                                                                                      gaaaTTTGCACAG.AAAAAGGGGGGGCGCGACGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCg           <  1:1358800/99‑1 (MQ=255)
                                                                                          tttGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACAt       >  2:825672/1‑101 (MQ=255)
                                                                                           ttGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATc      <  2:626087/101‑1 (MQ=255)
                                                                                            tGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATcc     >  2:1178064/1‑101 (MQ=255)
                                                                                             gCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCa    >  1:2645177/1‑101 (MQ=255)
                                                                                             gCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCa    >  1:4416253/1‑101 (MQ=255)
                                                                                             gCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCa    <  2:1306290/101‑1 (MQ=255)
                                                                                              cacaGC.AAAAAGGGGGGCGCGAAG.CCCCTTTTTTTCCGTTAGCGAAACAGCACCAGCGCGCCGGGCGACAACGCCACCCGCGTCTGTGTGCCGACTTCCAg   >  2:3638558/1‑101 (MQ=255)
                                                                                               acaGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGc  >  1:394597/1‑101 (MQ=255)
                                                                                                    |                                                                                                 
CGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAAGGGGGGCGCGAAGCCCCCCTTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGC  >  NC_014034/2044170‑2044367

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG