Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,057,828 Δ1 bp 16.9% intergenic (‑594/‑308) RCAP_rcc01900 ← / → RCAP_rcc01901 hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,057,8280G→.16.9% 72.8 ‑0.2 ‑0.3136intergenic (‑594/‑308)RCAP_rcc01900/RCAP_rcc01901hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (13/10):  ref base (60/53):  total (73/63)
Fisher's exact test strand distribution p-value = 8.22e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.04e-01

Only 100 of 156 total aligned reads displayed.

GTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CCGCG.CGC.AGC.G.TTCCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057732‑2057913
                                                                                                 |                                                                                          
gTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.Cc.cg.cgc                                                                                      >  2:3062531/1‑101 (MQ=255)
 tCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.Cc.cg.cgcc                                                                                     >  2:4121195/1‑100 (MQ=255)
 tCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.Cc.cg.cgcc                                                                                     >  2:358152/1‑100 (MQ=255)
 tCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.Cc.cg.cgcc                                                                                     <  1:3359170/101‑2 (MQ=255)
  ccTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.Cc.cg.cgccg                                                                                    >  1:418313/1‑99 (MQ=255)
  ccTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.Cc.cg.cgccg                                                                                    >  1:4229868/1‑99 (MQ=255)
   cTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGc                                                                                  >  2:3675111/1‑101 (MQ=255)
   cTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGc                                                                                  >  2:1518090/1‑101 (MQ=255)
      tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACCAT.CC.CG.CGC.CGcaag                                                                               >  1:129673/1‑98 (MQ=255)
      tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaag                                                                               <  1:2775956/101‑4 (MQ=255)
      tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaag                                                                               <  1:3015557/101‑4 (MQ=255)
       ttACCGCCCATTAGCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagc                                                                              <  2:1800576/101‑5 (MQ=255)
       ttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagc                                                                              <  2:1724051/101‑5 (MQ=255)
       ttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagc                                                                              <  1:1898453/101‑5 (MQ=255)
        tACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcg                                                                             >  1:4435652/1‑96 (MQ=255)
         aCCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgt                                                                            >  1:821461/1‑95 (MQ=255)
          ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgtt                                                                           <  1:3630583/101‑8 (MQ=255)
          ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgtt                                                                           <  2:2256118/101‑8 (MQ=255)
           cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgttt                                                                          >  1:2695265/1‑93 (MQ=255)
           cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgttt                                                                          >  1:369408/1‑93 (MQ=255)
           cGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgttt                                                                          >  2:449994/1‑93 (MQ=255)
            gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgtttc                                                                         <  1:4265518/101‑10 (MQ=255)
            gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgtttc                                                                         <  1:836971/101‑10 (MQ=255)
            gCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CC.CG.CGC.CGcaagcgtttc                                                                         >  2:2014778/1‑92 (MQ=255)
                    aCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCgg                                                                    >  1:914394/1‑101 (MQ=255)
                    aCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGCGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCgg                                                                    <  1:3111122/101‑1 (MQ=255)
                     cccTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGc                                                                   >  1:1022663/1‑101 (MQ=255)
                       cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCAt                                                                 <  2:4103195/101‑1 (MQ=255)
                       cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCAt                                                                 <  2:1362119/101‑1 (MQ=255)
                       cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCAt                                                                 <  1:1761062/101‑1 (MQ=255)
                        tGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCacc                                                                >  2:945897/1‑99 (MQ=255)
                            tCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTc                                                            >  1:2235624/1‑101 (MQ=255)
                            tCGGCGCGCGGAATTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTc                                                            >  1:3191686/1‑101 (MQ=255)
                             cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCt                                                           >  2:1397781/1‑101 (MQ=255)
                             cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCt                                                           <  2:1638213/101‑1 (MQ=255)
                              ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCtt                                                          <  1:897343/101‑1 (MQ=255)
                              ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCtt                                                          <  1:2810517/101‑1 (MQ=255)
                               gcgcgcGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTg                                                         <  2:2628535/101‑1 (MQ=255)
                                 gcgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.c                                                      <  1:3171291/101‑1 (MQ=255)
                                  cgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.cc                                                     <  1:2492290/101‑1 (MQ=255)
                                   gcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.ccc                                                    <  2:2496259/101‑1 (MQ=255)
                                     ggACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.ccccc                                                  <  1:1095787/101‑1 (MQ=255)
                                      gccTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.cccccc                                                 <  2:3536416/99‑2 (MQ=255)
                                      gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTgagatcgg                                                  >  1:3594459/1‑94 (MQ=255)
                                      gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.cccccc                                                 >  2:3401969/1‑100 (MQ=255)
                                      gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.cccccc                                                 <  1:237614/101‑2 (MQ=255)
                                      gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.cccccc                                                 >  1:33876/1‑100 (MQ=255)
                                       gcTTCGGGGCGGCGGGGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.ccccccg                                                <  2:3990397/100‑3 (MQ=255)
                                       acgtCGGGGCGGCGTTGGGCCGCTTGCGATCCTGCGTTTCCGGTTTGTCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGT.ccccccg                                                <  2:2212249/98‑3 (MQ=255)
                                       aCTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.ccccccg                                                >  2:1498884/1‑99 (MQ=255)
                                       aCTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.ccccccc                                                >  1:2882467/1‑99 (MQ=255)
                                         ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.ccccccgcc                                              <  2:867080/101‑5 (MQ=255)
                                         ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.ccccccgcc                                              >  1:3102527/1‑97 (MQ=255)
                                         ttCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGG.ccccccgcc                                              >  2:1345596/1‑97 (MQ=255)
                                            ggggCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGCGG.TTCCGAACCGGCATCATTCTTGG.CCCCCCCCcggg                                           >  1:1844841/1‑100 (MQ=255)
                                                cggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACaa                                        >  2:3263529/1‑101 (MQ=255)
                                                 ggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAAt                                       >  1:3487144/1‑101 (MQ=255)
                                                 ggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAAt                                       >  2:3214528/1‑101 (MQ=255)
                                                  gcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATc                                      <  1:1045744/101‑1 (MQ=255)
                                                   tggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCa                                     <  1:154243/100‑1 (MQ=255)
                                                    ggcggGGTTCTTGCGATCCTGCGTATCCGGATTGTCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCaa                                    >  2:154222/1‑100 (MQ=255)
                                                    ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                    >  2:729349/1‑101 (MQ=255)
                                                    ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                    <  2:172016/101‑1 (MQ=255)
                                                      cggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACgg                                  >  2:2419610/1‑101 (MQ=255)
                                                      cggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACgg                                  <  1:4435145/101‑1 (MQ=255)
                                                      cggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAAgg                                  >  2:2854038/1‑101 (MQ=255)
                                                       ggggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGCACCGGCATCATTCTTGGCCCCCCCCCGGGACAATCACGgc                                 >  1:1506127/1‑101 (MQ=255)
                                                        gggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgcc                                <  1:1150171/101‑1 (MQ=255)
                                                         ggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgccg                               >  1:2491620/1‑101 (MQ=255)
                                                          gCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgccgc                              <  2:316822/101‑1 (MQ=255)
                                                           ctctTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACAgccgcc                             >  1:2944158/1‑101 (MQ=255)
                                                            tctTGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCt                            >  2:3208340/1‑101 (MQ=255)
                                                            tctTGCGATCCTCCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCt                            >  1:355174/1‑101 (MQ=255)
                                                               tGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         <  1:1655774/101‑1 (MQ=255)
                                                               tGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         <  1:3340771/101‑1 (MQ=255)
                                                               tGCGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         >  1:1989796/1‑101 (MQ=255)
                                                                 cGATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTg                       <  1:1409731/101‑1 (MQ=255)
                                                                  gATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGt                      >  2:4060766/1‑101 (MQ=255)
                                                                  gATCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGt                      <  2:33863/101‑1 (MQ=255)
                                                                    tCCTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                    >  2:1593571/1‑101 (MQ=255)
                                                                    tCCTGCGTTTCCGGATGGGCGACAGTCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                    <  2:1098116/101‑1 (MQ=255)
                                                                     ccTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTcc                   >  1:214029/1‑101 (MQ=255)
                                                                      cTGCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCg                  >  2:1399377/1‑101 (MQ=255)
                                                                        gCGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGcc                >  1:4227015/1‑101 (MQ=255)
                                                                         cGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCa               <  2:369369/101‑1 (MQ=255)
                                                                         cGTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCa               <  2:824082/101‑1 (MQ=255)
                                                                         cGTTTCCGGATTGCCAACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCa               <  2:1137425/101‑1 (MQ=255)
                                                                          gTTTCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt              >  1:2103178/1‑101 (MQ=255)
                                                                           tttCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAtt             <  2:1085681/101‑1 (MQ=255)
                                                                           tttCCGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAtt             >  2:4247806/1‑101 (MQ=255)
                                                                             tCCGGATTGGCGAAAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGc           <  2:2696099/101‑1 (MQ=255)
                                                                              ccGGATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCg          >  2:1223077/1‑101 (MQ=255)
                                                                                ggATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        >  1:1727618/1‑101 (MQ=255)
                                                                                 gATTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATc       <  1:3038612/101‑1 (MQ=255)
                                                                                  aTTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      <  2:1022578/101‑1 (MQ=255)
                                                                                  aTTGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      >  1:629665/1‑101 (MQ=255)
                                                                                   ttGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaa     <  1:4121455/101‑1 (MQ=255)
                                                                                    tGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa    >  1:3604498/1‑101 (MQ=255)
                                                                                    tGGCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa    >  1:4284959/1‑101 (MQ=255)
                                                                                     ggCGACAATCCCGCGCCGCAAGC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCCTTGTTCCGCCATTGCGATCAAAg   >  1:411341/1‑101 (MQ=255)
                                                                                                 |                                                                                          
GTCCTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CCGCG.CGC.AGC.G.TTCCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057732‑2057913

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG