Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,180,242 Δ1 bp 100% coding (931/1314 nt) RCAP_rcc02022 → polyols ABC transporter periplasmic polyols‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,180,2420A→.100.0% 722.2 162coding (931/1314 nt)RCAP_rcc02022polyols ABC transporter periplasmic polyols‑binding protein
Reads supporting (aligned to +/- strand):  new base (74/88):  ref base (0/0):  total (74/88)

Only 100 of 180 total aligned reads displayed.

GCCGATCATGTCGGCTTCG.GCTGGC.CCGAACAAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGAACGTGCCCC  >  NC_014034/2180143‑2180338
                                                                                                      |                                                                                                
 ccGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaa                                                                                                 <  1:2870836/101‑1 (MQ=255)
  cGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaa                                                                                                <  1:296291/101‑1 (MQ=255)
   gATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaa                                                                                               >  2:337950/1‑101 (MQ=255)
   gATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaa                                                                                               <  2:2511219/101‑1 (MQ=255)
   gATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaa                                                                                               >  1:4287896/1‑101 (MQ=255)
    aTCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaag                                                                                              >  1:2198123/1‑100 (MQ=255)
     tCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaagg                                                                                             <  2:16157/101‑3 (MQ=255)
      cATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggc                                                                                            <  1:2259268/101‑4 (MQ=255)
        tGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCg                                                                                         >  2:2235592/1‑101 (MQ=255)
        tGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCg                                                                                         <  1:1955324/101‑1 (MQ=255)
          tcggcttcgcgcTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAg                                                                                       <  1:2225828/101‑1 (MQ=255)
           cggcttcgcgcTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAgg                                                                                      >  1:1796236/1‑101 (MQ=255)
           cggcttcgcgcTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAgg                                                                                      <  1:1902206/101‑1 (MQ=255)
             gcttcgcgcTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcg                                                                                    >  2:749748/1‑101 (MQ=255)
             gcttcgcgcTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcg                                                                                    <  1:3776518/101‑1 (MQ=255)
               ttcgcgctGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcggc                                                                                  <  2:3398055/96‑1 (MQ=255)
                tcgcgctGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGgcggcg                                                                                 <  1:2251500/97‑1 (MQ=255)
                 cgcgctGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGgcgc                                                                                <  1:4020901/98‑1 (MQ=255)
                  gcgctGGCGCCGAACAAGGCCGGTGTCGCCTAGCATGGCAACTTGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCt                                                                               <  1:308864/99‑1 (MQ=255)
                    gctGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGa                                                                             >  1:3137689/1‑101 (MQ=255)
                     ctggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaa                                                                            <  1:4245694/95‑1 (MQ=255)
                     ctggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaa                                                                            <  2:1265338/95‑1 (MQ=255)
                      tggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaaa                                                                           <  1:1359758/96‑1 (MQ=255)
                       ggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAt                                                                          <  1:1673564/97‑1 (MQ=255)
                        gcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAtt                                                                         >  2:61652/4‑101 (MQ=255)
                         cgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtc                                                                        <  2:289519/99‑1 (MQ=255)
                          gccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcg                                                                       >  1:1129031/2‑101 (MQ=255)
                            cGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtc                                                                     <  2:3033654/101‑1 (MQ=255)
                              aaCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa                                                                   <  2:3323042/101‑1 (MQ=255)
                              aaCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa                                                                   >  1:232184/1‑101 (MQ=255)
                               aCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGaa                                                                  <  1:1155141/101‑1 (MQ=255)
                                cAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAt                                                                 <  1:2578655/101‑1 (MQ=255)
                                 aaggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATg                                                                <  2:1063915/96‑1 (MQ=255)
                                 aaggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATg                                                                >  2:1337586/6‑101 (MQ=255)
                                  aggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATgg                                                               >  2:3657944/5‑101 (MQ=255)
                                  aggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATgg                                                               <  2:563232/97‑1 (MQ=255)
                                   ggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATggg                                                              <  2:4029359/98‑1 (MQ=255)
                                     ccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCg                                                            >  1:2791511/2‑101 (MQ=255)
                                      cGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGa                                                           >  2:2741339/1‑101 (MQ=255)
                                       ggTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAc                                                          >  1:3899278/1‑101 (MQ=255)
                                       ggTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAc                                                          <  2:1939847/101‑1 (MQ=255)
                                       ggTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAc                                                          >  2:2503018/1‑101 (MQ=255)
                                          gtcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGTCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTc                                                       >  1:3756298/6‑101 (MQ=255)
                                          gtcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTc                                                       >  2:2826601/6‑101 (MQ=255)
                                          gtcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTc                                                       <  2:4325090/96‑1 (MQ=255)
                                          gtcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTc                                                       >  1:3262328/6‑101 (MQ=255)
                                           tcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTcc                                                      <  2:2694708/97‑1 (MQ=255)
                                           tcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTcc                                                      <  1:337985/97‑1 (MQ=255)
                                             gccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCaa                                                    <  2:4417226/99‑1 (MQ=255)
                                              ccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAg                                                   >  1:1403877/2‑101 (MQ=255)
                                              ccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAg                                                   <  2:2105039/100‑1 (MQ=255)
                                              ccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAg                                                   >  2:3126942/2‑101 (MQ=255)
                                               cAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAgg                                                  >  1:1232179/1‑101 (MQ=255)
                                               cAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAgg                                                  <  2:3878927/101‑1 (MQ=255)
                                                aaGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGa                                                 <  2:2796369/101‑1 (MQ=255)
                                                aaGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGa                                                 <  1:443233/101‑1 (MQ=255)
                                                 aGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGAc                                                >  1:444469/1‑101 (MQ=255)
                                                  gCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACt                                               >  1:1620151/1‑101 (MQ=255)
                                                   cATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTa                                              <  1:761519/101‑1 (MQ=255)
                                                      ggaaaCTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTacac                                           <  1:3635547/98‑1 (MQ=255)
                                                      ggCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTacac                                           <  1:3346206/101‑1 (MQ=255)
                                                        cAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCg                                         >  2:4136640/1‑101 (MQ=255)
                                                        cAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCg                                         >  1:3045158/1‑101 (MQ=255)
                                                         aaCTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGc                                        >  1:3986980/1‑101 (MQ=255)
                                                             ggCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCtt                                    <  1:634387/101‑1 (MQ=255)
                                                             ggCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCtt                                    <  2:3756045/101‑1 (MQ=255)
                                                              gcgctGGGCGTGGAA.ACTCGCC.ACTCCGGCGGGCACGC.AAAAGGCCGAGACGGCGCTTTAATTTATCGAAAGGGCGACCCCCAAGAACTACACCGCCCTTg                                   <  2:3049040/98‑1 (MQ=255)
                                                               ctctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGt                                  <  2:1552486/101‑1 (MQ=255)
                                                               ctctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTACAAGGACTACAACACCCttct                                  >  2:621360/1‑99 (MQ=255)
                                                               ctctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTAGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGt                                  >  2:834952/1‑101 (MQ=255)
                                                                tctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTc                                 >  2:3017668/1‑101 (MQ=255)
                                                                tctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTc                                 >  1:1112989/1‑101 (MQ=255)
                                                                 ctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCg                                <  1:3004484/101‑1 (MQ=255)
                                                                   gggCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGcc                              <  2:3262096/101‑1 (MQ=255)
                                                                   gggCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGcc                              >  2:242682/1‑101 (MQ=255)
                                                                     gCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAg                            <  2:618695/101‑1 (MQ=255)
                                                                       cTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCa                          <  1:3502665/101‑1 (MQ=255)
                                                                          ggaaccTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTAGTCGAATGGGCGACCTCNAAGGACTACCACGCCCTTGTCGCCAGCaag                        >  1:1324513/5‑101 (MQ=255)
                                                                           gaaccTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaagg                       >  2:2380047/4‑101 (MQ=255)
                                                                           gaaccTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaagg                       <  1:4136900/98‑1 (MQ=255)
                                                                                tCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGc                  <  2:232157/101‑1 (MQ=255)
                                                                                tCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGc                  >  1:606396/1‑101 (MQ=255)
                                                                                  cgccaTTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCt                 <  2:3544373/97‑1 (MQ=255)
                                                                                    ccaTTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTgg               <  2:3029232/99‑1 (MQ=255)
                                                                                    ccaTTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTgg               <  2:1714604/99‑1 (MQ=255)
                                                                                    ccaTTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTgg               <  2:422242/99‑1 (MQ=255)
                                                                                      aTTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGc             <  1:2826808/101‑1 (MQ=255)
                                                                                      aTTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGc             >  2:1417434/1‑101 (MQ=255)
                                                                                      aTTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGc             <  2:3452052/101‑1 (MQ=255)
                                                                                       ttCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCg            <  2:3757001/101‑1 (MQ=255)
                                                                                         ccGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGaa          <  2:3922081/101‑1 (MQ=255)
                                                                                          cggcggGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGAAc         <  1:1753340/101‑1 (MQ=255)
                                                                                           ggcggGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGAACg        <  1:3059757/101‑1 (MQ=255)
                                                                                            gcggGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGAACGt       <  2:1232086/101‑1 (MQ=255)
                                                                                            gcggGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGATGGGCGAACGt       >  2:3165453/1‑101 (MQ=255)
                                                                                             ggggCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCACCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGAACGTg      <  2:2052658/100‑1 (MQ=255)
                                                                                              gggCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGAACGTGc     >  2:2344246/1‑101 (MQ=255)
                                                                                                gCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGAACGTGccc   <  1:242709/101‑1 (MQ=255)
                                                                                                gCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGAACGTGccc   <  1:4345673/101‑1 (MQ=255)
                                                                                                gCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGAACGTGccc   >  1:964653/1‑101 (MQ=255)
                                                                                                      |                                                                                                
GCCGATCATGTCGGCTTCG.GCTGGC.CCGAACAAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAAGGAAGGCTGGGCGAACGTGCCCC  >  NC_014034/2180143‑2180338

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG