Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,185,902	Δ1 bp	100%	intergenic (+175/‑330)	mtlK → / → RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,185,902	0	T→.	100.0%	271.9	66	intergenic (+175/‑330)	mtlK/RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (23/43):  ref base (0/0):  total (23/43)

CGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCT.CCG.CCCC.GCACCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGCCCGCCGGGTCTC  >  NC_014034/2185804‑2185988                                                                                                        |                                                                                        cGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCT.CCGCCCCC.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGttt                                                                                       <  2:1924428/101‑1 (MQ=255)  gCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCT.CCGCCCCC.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGtttt                                                                                      <  2:2471362/101‑1 (MQ=255)   ccTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCT.CCGCCCCC.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGttttg                                                                                     <  2:1553172/101‑2 (MQ=37)     tCGGCGGTGCCGAGGCGACCCTGCGGGGGGGCC.CCC.CCCC.CCCCCCCCCCGCGGTTTTTTCCAAAAAAAAAAAAGGGGTTTCCCCCCCGGCCCTTGttttt                                                                                     >  2:2844254/1‑101 (MQ=255)         cggTGCCGAGGCGACCCTGC..GGGGGCT.CCGCCCCC.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGca                                                                              <  1:2978375/101‑1 (MQ=37)         cggTGCCGAGGCGACCCTGC..GGGGGCT.CCGCCCCC.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGca                                                                              <  2:166896/101‑1 (MQ=37)           gTGCCGAGGCGACCCTGC..GGGGGCT.CCGCCCCC.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGcaca                                                                            <  1:2327872/101‑1 (MQ=37)             gCCGAGGCGCCCCTGC..GGGGGCT.CCGCCCCC.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAg                                                                          <  1:403761/101‑1 (MQ=25)              cctgtgCGTCCTTGC..GGGGGCTCCCC.CCCC.GCA.CCCCCGGCTTATTTATCCCAAGTAAATGCAGCGGCTTGCCCCTAAGGCCTTG.TTTTGATGGCACAAGa                                                                         <  2:3569230/96‑1 (MQ=16)               cGAGGCGACCCTGC..GGGGGCT.CCGCCCCC.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGaa                                                                        <  1:2699962/101‑1 (MQ=37)                gAGGCGACCCTGC..GGGGGCT.CCGCCCCC.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGaaa                                                                       <  1:4188859/101‑1 (MQ=37)                 aGGCGACCCTGC..GGGGGCT.CCGCCCCC.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAAc                                                                      <  2:2249634/101‑1 (MQ=37)                   gCGTCCATGC..GGGGGCT.CCGCCCCC.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGc                                                                    <  1:736742/101‑1 (MQ=25)                   gCGACCCTGC..GGGGGCT.CCGCCCCC.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGc                                                                    <  2:1172199/101‑1 (MQ=37)                    cGACCCTGC..GGGGGCT.CCGCCCCC.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGcc                                                                   <  1:423110/101‑1 (MQ=37)                     gACCCTGC..GGGGGCT.CCGCCCCC.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCg                                                                  <  2:287363/101‑1 (MQ=37)                      aCCCTGC..GGGGGCT.CCGCCCCC.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGa                                                                 <  2:898529/101‑1 (MQ=37)                       cccTGC..GGGGGCT.CCGCCCCC.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAATCGCCGaa                                                                <  2:917415/101‑1 (MQ=25)                       cccTGC..GGGGGCT.CCGCCCCC.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGaa                                                                <  1:505184/101‑1 (MQ=37)                         cTGC..GGGGGCT.CCGCCCCC.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCCTG.TTTTGATGGGACAAGAAAAGCCGaaac                                                              >  2:3504476/1‑99 (MQ=21)                          tGC..GGGGGCT.CCGCCCCC.GCA.CCCCCGGCGTATTTATCCCAAGGAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCg                                                             <  2:1059854/101‑1 (MQ=25)                           gC..GGGGGCT.CCG.CCCCAGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.c                                                           >  2:1030281/1‑101 (MQ=37)                            c..GGGGGCT.CCGCCCCC.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGACGAACCG.cc                                                          >  2:778582/1‑101 (MQ=25)                               gggggCT.CCGCCCCC.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.ccc                                                         <  1:2844461/101‑1 (MQ=37)                                      tccgccccc.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCG.CCCCctttc                                                   <  1:1933459/96‑3 (MQ=37)                                       ccgccccc.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCa                                                   <  2:815708/97‑1 (MQ=37)                                           cccc.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCtt                                               <  1:571069/101‑1 (MQ=38)                                            ccc.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTg                                              <  2:4164453/101‑1 (MQ=38)                                            ccc.GCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTg                                              <  1:869726/101‑1 (MQ=38)                                                 gcacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCt                                           <  2:392060/98‑1 (MQ=38)                                                  cacccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTc                                          >  1:2919293/3‑101 (MQ=39)                                                   ccccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCCCCTTGGCTCa                                         <  2:2980303/101‑1 (MQ=38)                                                   acccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCa                                         >  1:175090/2‑101 (MQ=255)                                                    cccccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCaa                                        <  1:788341/101‑1 (MQ=255)                                                      cccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAat                                      <  2:2032482/101‑1 (MQ=255)                                                      cccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAat                                      <  1:1137702/101‑1 (MQ=255)                                                       ccGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTCATGGTACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCCAata                                     >  2:1769438/1‑101 (MQ=255)                                                        cGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAatat                                    <  1:2021392/101‑1 (MQ=255)                                                         ggCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATc                                   <  1:3464580/101‑1 (MQ=255)                                                         ggCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATc                                   <  1:2033760/101‑1 (MQ=255)                                                          gCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATcc                                  <  1:319908/101‑1 (MQ=255)                                                           cGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATccc                                 >  2:1196191/1‑101 (MQ=255)                                                           cGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATccc                                 >  1:2301058/1‑101 (MQ=255)                                                            gTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCg                                <  1:3264854/101‑1 (MQ=255)                                                            gTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCg                                <  1:2371912/101‑1 (MQ=255)                                                             tATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCgg                               <  1:4145593/101‑1 (MQ=255)                                                              aTTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCggg                              >  1:697829/1‑101 (MQ=255)                                                                ttATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCggggg                            >  1:2823282/1‑101 (MQ=255)                                                                  aTCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCAGAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTc                          >  1:3764180/1‑101 (MQ=38)                                                                  aTCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTc                          <  1:2276389/101‑1 (MQ=255)                                                                  aTCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTc                          >  2:3410970/1‑101 (MQ=255)                                                                   tCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTcc                         >  2:815041/1‑101 (MQ=255)                                                                   tCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTcc                         >  2:4077041/1‑101 (MQ=255)                                                                   tCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTcc                         <  1:2583308/101‑1 (MQ=255)                                                                   tCCCAAGAAAAAGCAGCGGCTTGCCCCCAATGCCTTG.TTTTGATGGCACAAGAAACGCCCAACCGCCCCCTTTCACCTTGGCTCAAATATCCCgcgggcgc                         >  2:4387500/1‑98 (MQ=255)                                                                    cccAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCg                        <  1:3946348/101‑1 (MQ=255)                                                                    cccAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCg                        <  2:4008519/101‑1 (MQ=255)                                                                      cAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCggg                      <  2:3658013/101‑1 (MQ=255)                                                                        aGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCggggg                    >  1:54756/1‑101 (MQ=255)                                                                         gAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAAAGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCCGGGGTCCGGGGGc                   >  2:2159751/1‑101 (MQ=255)                                                                          aaaaaGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCg                  <  2:3077307/101‑1 (MQ=255)                                                                           aaaaGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCgg                 >  1:11863/1‑101 (MQ=255)                                                                            aaaGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGa                <  1:1269312/101‑1 (MQ=255)                                                                             aaGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAg               <  2:4252996/101‑1 (MQ=255)                                                                               gcagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGcc             >  2:1542320/1‑101 (MQ=255)                                                                                 agcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGcccc           >  2:3171484/1‑100 (MQ=37)                                                                                  gcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGccccc          >  1:3410807/1‑99 (MQ=37)                                                                                    ggCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGcccccgg        >  2:2370979/1‑97 (MQ=25)                                                                                         gCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGcccccggcgggg   >  2:2179669/1‑92 (MQ=21)                                                                                          ccccGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGcccccggcggggg  >  2:3248286/1‑91 (MQ=21)                                                                                          ccccGAAGGCCTTG.TTTTGATGGCAAAAGAACCGCCGACCCGCCCCCTTTCCCCTTGGCTCAAATATCCGGGGGGTCGGGGGGCGGAGcccccggcgggtc  <  2:1734320/101‑11 (MQ=16)                                                                                                        |                                                                                        CGCCTCGGCGGTGCCGAGGCGACCCTGC..GGGGGCT.CCG.CCCC.GCACCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGCCCGCCGGGTCTC  >  NC_014034/2185804‑2185988 Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG