Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,236,666 Δ1 bp 2.5% intergenic (+17/‑112) RCAP_rcc02069 → / → RCAP_rcc02070 hypothetical protein/ArsR family transcriptional regulator/protein tyrosine phosphatase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,236,6660C→. 2.5% 4.4 0.0 ‑0.1158intergenic (+17/‑112)RCAP_rcc02069/RCAP_rcc02070hypothetical protein/ArsR family transcriptional regulator/protein tyrosine phosphatase
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (84/69):  total (87/71)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.81e-01

Only 100 of 203 total aligned reads displayed.

CCTGGGAACGCACGCTGGGCGATGCCGAGGATTTCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCTTCCTGT  >  NC_014034/2236567‑2236766
                                                                                                     |                                                                                                    
  tGGGAACGCACGCTGGGCGATGCCGAGGATTTCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.cccc                                                                                                   >  2:3260172/1‑101 (MQ=255)
   gggAACGCACGCTGGGCGATGCCGAGGATTTCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.ccccc                                                                                                  >  2:2356254/1‑101 (MQ=255)
   gggAACGCACGCTGGGCGATGCCGAGGATTTCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.ccccc                                                                                                  >  1:2990576/1‑101 (MQ=255)
        cggccggggggCGAGGCCGGGTTTTCCCCCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCt                                                                                             <  1:3872194/93‑1 (MQ=255)
        cgcgcgcGGGGCGAGCCCGGGTTTTCCCCCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCt                                                                                             <  2:3466169/101‑1 (MQ=255)
         gcacgcTGGGCGATGCCGAGGATTTCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTg                                                                                            >  2:2341649/1‑101 (MQ=255)
          cacgcTGGGCGATGCCGAGGATTTCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCCGc                                                                                           >  2:2476002/1‑101 (MQ=255)
             gcTGGGCGATGCCGAGGATTTCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCCGcccc                                                                                        >  1:1521647/1‑101 (MQ=255)
              gggggCGATGCGGAGGTTTCCCCCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGTTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGccccc                                                                                       <  1:2635570/99‑1 (MQ=255)
               ggggCGATGCGGATGTTTTCCCCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGCCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCg                                                                                      <  2:2998245/100‑1 (MQ=255)
                   cGATGCCGAGGATTTCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAAc                                                                                  >  1:178306/1‑101 (MQ=255)
                    gtggCCGAGGTTTCCCCCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACg                                                                                 <  2:793531/98‑1 (MQ=255)
                     aTGCCGAGGATTCCCCCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACgg                                                                                <  1:2175871/101‑1 (MQ=255)
                      tGCCGAGGATTTCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCCGCCGCC.TG.CCCCCCCCCCGCCCCCCCACCggg                                                                               >  1:447408/1‑101 (MQ=255)
                      ggCCGAGGTTTCCCCCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACggg                                                                               <  1:1886239/100‑1 (MQ=255)
                       gCCGAGGATTTCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACgggg                                                                              >  2:2760765/1‑100 (MQ=255)
                         cGAGGATTTCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCCGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCCACGGgcgc                                                                            >  1:3628698/1‑101 (MQ=255)
                           agtaTTTCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAg                                                                          >  1:1206219/4‑101 (MQ=255)
                            ggATTTCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCCGGCCGCGCTCTGACGCGCTCGGCCGCC.TG..CCCCCCCCTGCCCCCGCAACGGGGGCAggc                                                                        >  1:3692052/1‑99 (MQ=255)
                             gATTTCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTc                                                                        >  1:302088/1‑101 (MQ=255)
                                ttCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGc                                                                     >  1:3445023/1‑101 (MQ=255)
                                tccccccGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCCACGGGCGCAGTCTGc                                                                     <  1:3751305/97‑1 (MQ=255)
                                tccccccGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGc                                                                     <  2:3981389/97‑1 (MQ=255)
                                 tCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCCCCACGGGCGCAAGCTGcc                                                                    >  2:17559/1‑100 (MQ=255)
                                   aCCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGcaca                                                                  >  2:3172120/1‑101 (MQ=255)
                                     ccgccgCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGt                                                                <  2:113033/101‑1 (MQ=255)
                                      cgccgcTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTc                                                               <  1:3711076/101‑1 (MQ=255)
                                      cgccgcTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCCGCCCCCCCAACGGGCGCCGGCCGCCCAGTc                                                               >  1:1936884/1‑101 (MQ=255)
                                       gccgcTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTcc                                                              <  1:1615994/101‑1 (MQ=255)
                                       gccgcTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTcc                                                              <  1:1403968/101‑1 (MQ=255)
                                       gccgcTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTcc                                                              <  2:1043342/101‑1 (MQ=255)
                                       gccgcTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTcc                                                              >  2:4237900/1‑101 (MQ=255)
                                       gccgcTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTcc                                                              >  2:2020887/1‑101 (MQ=255)
                                       gccgcTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTcc                                                              >  2:2704334/1‑101 (MQ=255)
                                        ccgcTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCcg                                                             <  2:279415/101‑1 (MQ=255)
                                          gcTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCcggc                                                           >  2:591167/1‑101 (MQ=255)
                                          gcTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCcggc                                                           >  2:2678905/1‑101 (MQ=255)
                                          gcTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCcggc                                                           >  1:4140489/1‑101 (MQ=255)
                                           cTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCCACGGGCGCAGGCTGCACAGGCcggcg                                                          >  1:3062283/1‑101 (MQ=255)
                                              cGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCggcggcc                                                       >  2:310306/1‑100 (MQ=255)
                                               gAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGgcgc                                                      >  1:34148/1‑101 (MQ=255)
                                                aaGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCCGCCCCCGCAACGGGCGCAGTCTGCACAGGCCGGCGGCGcc                                                     >  1:136377/1‑101 (MQ=255)
                                                 ggcggcgctgcTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCt                                                    <  1:3555708/97‑1 (MQ=255)
                                                 aGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCt                                                    <  1:4347971/101‑1 (MQ=255)
                                                 aGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCt                                                    <  1:1910918/101‑1 (MQ=255)
                                                 aGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCt                                                    <  1:2105554/101‑1 (MQ=255)
                                                 aGCTGCGCTGCGGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCt                                                    <  2:2874440/101‑1 (MQ=255)
                                                 aGCTGCCCTGCGGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCCCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCt                                                    <  2:1094446/101‑1 (MQ=255)
                                                      cgctgcTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCCGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCCGGCGc                                               >  1:658864/1‑101 (MQ=255)
                                                       gctgggggCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGcc                                              <  1:2792601/95‑1 (MQ=255)
                                                        ctgctgGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGCCGCCg                                             >  2:1619384/1‑101 (MQ=255)
                                                         tgctgGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCgg                                            >  2:3316726/1‑101 (MQ=255)
                                                          gctgGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCggg                                           >  1:3490848/1‑100 (MQ=255)
                                                            tgGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TGCCCCCCCCCCTCCCCCCGCACGGGGCGCAGTCTCCACAGCCCGGCGGCCCCTTCCCCCGGAt                                          >  1:3582261/1‑101 (MQ=255)
                                                             ggCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTg                                        <  2:4152723/101‑1 (MQ=255)
                                                             ggCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTg                                        <  2:4068058/101‑1 (MQ=255)
                                                              gCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGt                                       <  2:1806673/101‑1 (MQ=255)
                                                               cgggccgggcCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTc                                      >  2:3917653/1‑101 (MQ=255)
                                                                gggccgggccGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCa                                     <  2:2638711/101‑1 (MQ=255)
                                                                 ggccgggccgCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCAt                                    >  2:2724974/1‑101 (MQ=255)
                                                                 ggccgggccgCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCAt                                    >  1:2160916/1‑101 (MQ=255)
                                                                 ggccgggccgCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCCCCGGATTGTCAt                                    >  1:2058508/1‑101 (MQ=255)
                                                                 ggccggcccgCCCTCTGCCGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCAt                                    <  2:4096122/101‑1 (MQ=255)
                                                                    cgggccgCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGaa                                 >  2:2062352/1‑101 (MQ=255)
                                                                    cgggcccCCCGCTGACGCGCTCGCCCCCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGaa                                 <  2:3536790/101‑1 (MQ=255)
                                                                       gccgCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGc                              >  2:4376807/1‑101 (MQ=255)
                                                                       gccgCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGc                              >  2:1035901/1‑101 (MQ=255)
                                                                        cccccctctGACGCGCCCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCt                             <  2:3070165/96‑1 (MQ=255)
                                                                         cgcgCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCtt                            <  1:3171989/101‑1 (MQ=255)
                                                                         cgcgCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCtt                            >  2:1914431/1‑101 (MQ=255)
                                                                           cgcTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCa                          <  2:2197310/101‑1 (MQ=255)
                                                                           ccctttGACCCGCTCGGCCGCCTTC.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTc                           <  2:3589095/96‑1 (MQ=38)
                                                                             ctctGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACt                        <  1:2373189/101‑1 (MQ=255)
                                                                             cgctGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACt                        <  2:1040501/99‑1 (MQ=255)
                                                                              tctGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTg                       >  2:3842324/1‑101 (MQ=255)
                                                                              tctGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTg                       <  2:361073/101‑1 (MQ=255)
                                                                               ctGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTgg                      <  1:441857/101‑1 (MQ=255)
                                                                                tgaccgcatCGGCCGCC.TG.CCCGCCCCCTGCCCCCGCAACGGCCGCAGTCTGCACAGTCCGGCGGCGCCCGTCGCCGGATTGTCATGAAAGCTTCACTGGa                     <  2:745139/93‑1 (MQ=37)
                                                                                tGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGa                     <  2:2847602/101‑1 (MQ=255)
                                                                                tGACGCGCTCGGCCGCC.TG.CCCCCCCCCCGCCCCCGCAACGGGCCCAGTCTGCACAGTCCGGCGGACCCTGTCGCCGGATTGGCACGAAACCTTCACTGGa                     >  1:4066392/1‑101 (MQ=255)
                                                                                gggcgcgCCCGGCCGCA.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGa                     <  2:2122376/98‑1 (MQ=255)
                                                                                 gccgccCTCGGCCCCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGAc                    <  1:17572/99‑1 (MQ=255)
                                                                                  cccngcNCGCCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACg                   <  1:563305/97‑1 (MQ=255)
                                                                                  aCGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACg                   <  2:1412067/101‑1 (MQ=255)
                                                                                   cgcgCGCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACgg                  <  1:3094190/101‑1 (MQ=255)
                                                                                    cccgtCGCCCCCC.TA.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACggg                 <  1:1130371/97‑1 (MQ=37)
                                                                                      gcgcGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACggggg               <  1:880163/98‑1 (MQ=255)
                                                                                      ccTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACggggg               <  2:843369/100‑1 (MQ=255)
                                                                                         cGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACgggggggt            >  1:2298137/1‑99 (MQ=255)
                                                                                         cGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGAt            <  1:1055089/101‑1 (MQ=255)
                                                                                          ggCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGGtt           >  1:1271191/1‑101 (MQ=255)
                                                                                           gccgcc.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTg          <  1:2704524/101‑1 (MQ=255)
                                                                                           gccgcc.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGAGGGGATTg          <  1:2468246/101‑1 (MQ=255)
                                                                                             cgcc.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCt        <  1:4238161/101‑1 (MQ=255)
                                                                                              gcc.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCtt       >  1:3300298/1‑101 (MQ=255)
                                                                                               cc.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGGTTGCTTc      >  1:4210220/1‑101 (MQ=255)
                                                                                               cc.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCTTc      >  2:3241788/1‑101 (MQ=255)
                                                                                                  tG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCTTCCt    <  1:591235/101‑1 (MQ=255)
                                                                                                  tG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCTTCCt    <  2:4445236/101‑1 (MQ=255)
                                                                                                     cccccccccTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCTTCCTGt  <  2:499124/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
CCTGGGAACGCACGCTGGGCGATGCCGAGGATTTCACCCGCCGCTTCGAAGCTGCGCTGCTGGCGGGCCGGGCCGCGCTCTGACGCGCTCGGCCGCC.TG.CCCCCCCCCTGCCCCCGCAACGGGCGCAGTCTGCACAGTCCGGCGGCGCCTGTCGCCGGATTGTCATGAAAGCTTCACTGGACGGGGGGATTGCTTCCTGT  >  NC_014034/2236567‑2236766

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG