Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,279,867 Δ1 bp 100% intergenic (‑424/+82) RCAP_rcc02119 ← / ← RCAP_rcc02120 type 12 family methyltransferase/ABC transporter ATP‑binding/periplasmic substrate‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,279,8670G→.100.0% 881.3 199intergenic (‑424/+82)RCAP_rcc02119/RCAP_rcc02120type 12 family methyltransferase/ABC transporter ATP‑binding/periplasmic substrate‑binding protein
Reads supporting (aligned to +/- strand):  new base (102/96):  ref base (1/0):  total (103/96)

Only 100 of 218 total aligned reads displayed.

TTTGGGCAAGTTAAAGGCGACGCAGGACGCTCTGATTGACGCGCGT.CCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCAGGGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGAAACGGCGTCAGGACGCCGTCTC  >  NC_014034/2279768‑2279963
                                                                                                    |                                                                                                
 ttGGGCAAGTTAAAGGCGACGCAGGACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCAgg                                                                                                 >  1:2940912/1‑101 (MQ=255)
  tGGGCAAGTTAAAGGCGACGCAGGACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCAggg                                                                                                >  2:1502837/1‑101 (MQ=255)
  tGGGCAAGTTAAAGGCGACGCAGGACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCAggg                                                                                                >  2:4337667/1‑101 (MQ=255)
     gCAAGTTAAAGGCGACGCAGGACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCAggggga                                                                                             <  1:4320179/101‑2 (MQ=255)
        aGTTAAAGGCGACGCAGGACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCAgggggatga                                                                                          <  2:2599439/101‑5 (MQ=255)
         gTTAAAGGCGACGCAGGACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGAc                                                                                        >  1:4044918/1‑101 (MQ=255)
          ttAAAGGCGACGCAGGACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGAcc                                                                                       >  2:4051726/1‑101 (MQ=255)
           tAAAGGCGACGCAGGACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACcg                                                                                      <  2:2564731/101‑1 (MQ=255)
            aaaGGCGACGCAGGACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACcgg                                                                                     >  1:1507211/1‑101 (MQ=255)
            aaaGGCGACGCAGGACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACcgg                                                                                     >  1:3442272/1‑101 (MQ=255)
            aaaGACGCCGCAGGACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACcgg                                                                                     >  2:3993680/1‑101 (MQ=255)
                 cGACGCAGGACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTc                                                                                <  1:2910367/101‑1 (MQ=255)
                   aCGCAGGACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCtt                                                                              <  1:4025720/101‑1 (MQ=255)
                   aCGCAGGACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCtt                                                                              <  2:2861285/101‑1 (MQ=255)
                   aCGCAGGACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCtt                                                                              <  2:2128056/101‑1 (MQ=255)
                   aCGCAGGACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCtt                                                                              <  2:2320630/101‑1 (MQ=255)
                    cGCAGGACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTg                                                                             <  2:1841262/101‑1 (MQ=255)
                    cGCAGGACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTg                                                                             <  2:1056089/101‑1 (MQ=255)
                     gCAGGACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGc                                                                            >  2:1668169/1‑101 (MQ=255)
                     gCAGGACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGc                                                                            >  1:2354752/1‑101 (MQ=255)
                     gCAGGACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGc                                                                            >  1:4412069/1‑101 (MQ=255)
                      cAGGACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCa                                                                           >  2:1318389/1‑101 (MQ=255)
                      cAGGACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCa                                                                           >  2:4102289/1‑101 (MQ=255)
                      cAGGACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCCCCGCTTGAATGACATATCCGAAACTATGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCa                                                                           >  1:3899295/1‑101 (MQ=255)
                        ggACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACg                                                                         <  2:236475/101‑1 (MQ=255)
                        ggACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACg                                                                         <  2:1182427/101‑1 (MQ=255)
                         gACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACgg                                                                        <  2:3705586/101‑1 (MQ=255)
                         gACGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACgg                                                                        >  2:663483/1‑101 (MQ=255)
                          gcGCTCTGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGc                                                                       <  1:2629908/100‑1 (MQ=255)
                              tctGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGa                                                                   <  1:3501043/101‑1 (MQ=255)
                              tctGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGa                                                                   >  1:1028554/1‑101 (MQ=255)
                               ctGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGAt                                                                  >  1:909664/1‑101 (MQ=255)
                                tGATTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGAtc                                                                 <  1:36273/101‑1 (MQ=255)
                                  aTTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGAtctt                                                               <  1:649443/101‑1 (MQ=255)
                                  aTTGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGAtctt                                                               >  1:3357432/1‑101 (MQ=255)
                                    tGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGAtcttct                                                             >  1:2658473/1‑101 (MQ=255)
                                    tGACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGAtcttct                                                             >  1:4382787/1‑101 (MQ=255)
                                     gACGCGCGTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATcttctt                                                            <  2:2525574/101‑1 (MQ=255)
                                       cgcgcgTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCa                                                          <  1:3594007/101‑1 (MQ=255)
                                        gcgcgTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCaa                                                         >  1:1730700/1‑101 (MQ=255)
                                        gcgcgTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCaa                                                         >  1:3464934/1‑101 (MQ=255)
                                         cgcgTCCCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAg                                                        >  1:1821195/1‑101 (MQ=255)
                                           cgtccccTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAgcg                                                      <  2:809496/97‑1 (MQ=255)
                                            gtccccTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAgcgc                                                     <  2:1588357/98‑1 (MQ=255)
                                             tccccTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCa                                                    <  2:1002338/99‑1 (MQ=255)
                                             tccccTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCa                                                    <  1:2131253/99‑1 (MQ=255)
                                             tccccTGCAGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCa                                                    <  2:901663/99‑1 (MQ=255)
                                              ccccTGCGGGCAGAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCaa                                                   >  1:2428820/2‑101 (MQ=255)
                                              ccccTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCaa                                                   >  2:3400278/2‑101 (MQ=255)
                                              ccccTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCaa                                                   <  1:887288/100‑1 (MQ=255)
                                               cccTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAAt                                                  >  1:1296653/1‑101 (MQ=255)
                                                ccTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATg                                                 <  2:2463764/101‑1 (MQ=255)
                                                ccTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATg                                                 <  1:638936/101‑1 (MQ=255)
                                                  tGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGc                                               <  1:712930/101‑1 (MQ=255)
                                                   gCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCt                                              >  1:604578/1‑101 (MQ=255)
                                                    cGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCtt                                             <  1:2592813/101‑1 (MQ=255)
                                                    cGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCtt                                             >  2:3104875/1‑101 (MQ=255)
                                                     gggCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTg                                            <  2:3468648/101‑1 (MQ=255)
                                                       gCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTtcg                                          >  2:1754921/1‑101 (MQ=255)
                                                         aaaGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGcc                                        <  2:56373/101‑1 (MQ=255)
                                                          aaGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCg                                       >  2:3061477/1‑101 (MQ=255)
                                                            gcgcCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGAt                                     <  1:1989986/101‑1 (MQ=255)
                                                              gccgcTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTTCGCCGAtgg                                   >  1:2576979/1‑100 (MQ=255)
                                                              gccgcTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGAtgt                                   <  2:3930314/101‑1 (MQ=255)
                                                               ccgcTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGAtgtg                                  >  2:2053099/1‑101 (MQ=255)
                                                                 gcTTGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGcc                                <  2:1028466/101‑1 (MQ=255)
                                                                  cTTGAATGACATATCCGAAACTCTGCTTTTTCCAGGGGGG.TGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGccc                               >  1:315407/1‑101 (MQ=255)
                                                                   ttGAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCccg                              >  2:3241130/1‑101 (MQ=255)
                                                                     gAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCccggc                            >  2:3362588/1‑101 (MQ=255)
                                                                     gAATGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCccggc                            >  1:1687371/1‑101 (MQ=255)
                                                                       aTGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAGTCGCAATGGCTTGCGCCGATGTGCccggccg                          <  1:3974690/101‑1 (MQ=255)
                                                                       aTGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAANCGCAATGGCTTGCGCCGATGTGAccggaac                          >  1:2167367/1‑98 (MQ=255)
                                                                       aTGACATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCccgggcg                          >  2:3308277/1‑101 (MQ=255)
                                                                          aCATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGaa                       <  1:1717664/101‑1 (MQ=255)
                                                                          aCATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGaa                       >  1:3838914/1‑101 (MQ=255)
                                                                          aCATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGaa                       <  1:4102549/101‑1 (MQ=255)
                                                                           cATATCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGaaa                      <  2:648495/101‑1 (MQ=255)
                                                                            atatCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGAAAc                     <  2:3777012/101‑1 (MQ=255)
                                                                            atatCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGAAAc                     >  1:4071024/1‑101 (MQ=255)
                                                                            atatCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGAAAc                     <  2:113606/101‑1 (MQ=255)
                                                                             tatCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGAAACg                    <  1:1318486/101‑1 (MQ=255)
                                                                              atCCGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGAAACgg                   >  1:3035096/1‑101 (MQ=255)
                                                                                ccGAAACTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGAAACGGCg                 >  1:112706/1‑101 (MQ=255)
                                                                                   aaaCTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGAAACGGCGTCa              <  1:2344246/101‑1 (MQ=255)
                                                                                     aCTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGAAACGGCGTCAgg            <  2:877620/101‑1 (MQ=255)
                                                                                     aCTCTGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGAAACGGCGTCAgg            >  1:1914178/1‑101 (MQ=255)
                                                                                       tctGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGTCGGAAACGGCGTCAGGAc          <  2:2937821/101‑1 (MQ=255)
                                                                                       tctGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGAAACGGCGTCAGGAc          >  1:249951/1‑101 (MQ=255)
                                                                                       tctGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGAAACGGCGGCAGGaa          >  2:2022939/1‑100 (MQ=255)
                                                                                        ctGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGAAACGGCGTCAGGACg         <  2:1975777/101‑1 (MQ=255)
                                                                                         tGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGAAACGGCGTCaaagacc        <  2:1730589/101‑7 (MQ=255)
                                                                                         tGCTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGAAACGGCGTCAGGACGc        <  2:2658301/101‑1 (MQ=255)
                                                                                           cTTTTTCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGAAACGGCGTCAGGACGCCg      >  1:838324/1‑101 (MQ=255)
                                                                                            tttttCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGAAACGGCGTCAGGACGCCGt     >  1:4153185/1‑101 (MQ=255)
                                                                                            tttttCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGAAACGGCGGCAGGACGCCgc     >  2:4180394/1‑100 (MQ=255)
                                                                                            tttttCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGAGCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGAAACGGCGTCAGGACTCCGt     <  1:1194313/101‑1 (MQ=255)
                                                                                              tttCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGAAACGGCGTCAGGACGCCGtct   <  2:3899041/101‑1 (MQ=255)
                                                                                               ttCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGAAACGGCGTCAGGACGCCGtctc  >  1:2756696/1‑101 (MQ=255)
                                                                                               ttCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGAAACGGCGTCAGGACGCCGtctc  <  1:3156794/101‑1 (MQ=255)
                                                                                               ttCCA.GGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGAAACGGCGTCAGGACGCCGtctc  >  1:471137/1‑101 (MQ=255)
                                                                                                    |                                                                                                
TTTGGGCAAGTTAAAGGCGACGCAGGACGCTCTGATTGACGCGCGT.CCCTGCGGGCAAAGCGCCGCTTGAATGACATATCCGAAACTCTGCTTTTTCCAGGGGGGATGACCGGCGGTCTTGCACGGCATGATCTTCTTCAAGCGCAATGGCTTGCGCCGATGTGCCCGGCCGGAAACGGCGTCAGGACGCCGTCTC  >  NC_014034/2279768‑2279963

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG