Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,420,239 Δ1 bp 100% intergenic (‑33/+159) RCAP_rcc02245 ← / ← cysE2 major membrane protein I/serine O‑acetyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,420,2390A→.100.0% 847.5 191intergenic (‑33/+159)RCAP_rcc02245/cysE2major membrane protein I/serine O‑acetyltransferase
Reads supporting (aligned to +/- strand):  new base (105/86):  ref base (0/0):  total (105/86)

Only 100 of 194 total aligned reads displayed.

GGCGTTCTTCACCGTGTTGAGCCGGTAGATCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGCAGTGATCGTGGGCCAGGATACGGCGCCCGTCTTCGTGGCATTGGCAAAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAGTTTGACACCGGTTCGGTCATTCACGTTCTCCTTCTTGGGG  >  NC_014034/2420141‑2420338
                                                                                                  |                                                                                                   
 gCGTTCTTCCCCGTGTTGAGCCGGTAGATCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGgcgc                                                                                               <  1:4420939/101‑1 (MQ=255)
  cGTTCTTCCCCGTTTTGAGCCGGTAGACCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCGcc                                                                                              <  1:1263009/101‑1 (MQ=255)
  cGTTCTTCCCCGTGTTGAGCCGGTAGACCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCGcc                                                                                              <  2:2254346/101‑1 (MQ=255)
  cGTTCTTCACCGTGTTGAGCCGGTAGATCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCGcc                                                                                              >  1:255177/1‑101 (MQ=255)
    ttcttcACCGTGTTGAGCCGGTAGATCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCGccgt                                                                                            >  2:4024082/1‑99 (MQ=255)
    ttcttcACCGTGTTGAGCCGGTAGATCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCGccgt                                                                                            >  1:204025/1‑99 (MQ=255)
    ttcttcACCGTGTTGAGCCGGTAGATCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCGccgt                                                                                            >  1:1964042/1‑99 (MQ=255)
    ttcttcACCGTGTTGAGCCGGTAGATCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCGccgt                                                                                            >  2:3545659/1‑99 (MQ=255)
    ttcttcACCGTGTTGAGCCGGTAGATCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCGccgc                                                                                            >  2:2185586/1‑99 (MQ=255)
       ttcCCCGTGTTGAGCCGGTAGACCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCtt                                                                                        <  1:2280654/101‑1 (MQ=255)
       ttcACCGTGTTGAGCCGGTAGATCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCtt                                                                                        >  1:1824266/1‑101 (MQ=255)
       ttcACCGTGTTGAGCCGGTAGATCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCtt                                                                                        >  1:1447718/1‑101 (MQ=255)
        tcccggttttGAGGCGGTAGGCCCCCGCCTCGGCCGGAAGCCATTGCAGGAAATGCCCCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTc                                                                                       <  1:98774/93‑1 (MQ=255)
        tcACCGTGTTGAGCCGGTAGATCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTc                                                                                       >  2:3952162/1‑101 (MQ=255)
         cACCGTGTTGAGCCGGTAGATCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCg                                                                                      >  1:2352856/1‑101 (MQ=255)
         cACCGTGTTGAGCCGGTAGATCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCg                                                                                      >  1:2590671/1‑101 (MQ=255)
             gtgtTGAGCCGGTAGATCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGc                                                                                  >  2:2929012/1‑101 (MQ=255)
                 tGAGCCGGTAGATCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTg                                                                              >  2:137922/1‑101 (MQ=255)
                 tGAGCCGGTAGACCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTg                                                                              <  2:1632320/101‑1 (MQ=255)
                  gAGCCGGTAGATCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGGCTTCGTGGCATTgg                                                                             >  2:1461391/1‑101 (MQ=255)
                   aGCGGGTAGACCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGc                                                                            <  1:1918850/101‑1 (MQ=255)
                   aGCCGGTAGATCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGc                                                                            >  1:702527/1‑101 (MQ=255)
                    gCCGGTAGACCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGCa                                                                           <  1:3226332/101‑1 (MQ=255)
                     cgggTAGACCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGCaa                                                                          <  2:540940/99‑1 (MQ=255)
                     ccGGTAGCCCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGCaa                                                                          <  2:2861234/101‑1 (MQ=255)
                     ccGGTAGATCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGCaa                                                                          <  1:2126089/101‑1 (MQ=255)
                        gTAGATCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGCaagct                                                                       >  1:1742727/1‑98 (MQ=255)
                         tAGATCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGCaagctg                                                                      >  2:2493136/1‑97 (MQ=255)
                          aGATCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTgg                                                                    >  1:1392695/1‑101 (MQ=255)
                           gATCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTggc                                                                   >  1:619950/1‑101 (MQ=255)
                            aTCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTggcg                                                                  >  2:367243/1‑101 (MQ=255)
                             cccccGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTggcgg                                                                 <  1:4314221/100‑1 (MQ=255)
                              ccccGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTggcggg                                                                >  2:519906/1‑101 (MQ=255)
                                ccGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTggcgggcg                                                              >  2:638483/1‑101 (MQ=255)
                                 cGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGgcgggggg                                                             >  1:3788633/1‑101 (MQ=255)
                                 cGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGgcgggcgg                                                             >  2:1084118/1‑101 (MQ=255)
                                  gCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGggcggc                                                            <  1:2890490/101‑1 (MQ=255)
                                   ccccGATCGGAAGCCATTGCAGGAAATTCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCa                                                           <  2:2707101/98‑1 (MQ=255)
                                   ccTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGgcgggcggga                                                           >  2:157809/1‑99 (MQ=255)
                                   ccTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCa                                                           <  1:2185725/101‑1 (MQ=255)
                                    cTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGGGGCAc                                                          >  1:571205/1‑101 (MQ=255)
                                    cTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCAc                                                          <  2:4135388/101‑1 (MQ=255)
                                      gcgccGGCACCCATTGAAGCAACTGCACCAGCGAGAGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTg                                                        <  2:585917/98‑1 (MQ=255)
                                      cGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTg                                                        <  2:4191904/101‑1 (MQ=255)
                                       gACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGt                                                       >  2:701967/1‑101 (MQ=255)
                                       gACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGt                                                       >  2:932905/1‑101 (MQ=255)
                                       gACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGt                                                       >  1:4176184/1‑101 (MQ=255)
                                          cGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGc                                                    >  1:48818/1‑101 (MQ=255)
                                          cGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGc                                                    >  1:1078417/1‑101 (MQ=255)
                                           ggAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGcc                                                   >  2:1543381/1‑101 (MQ=255)
                                           ggAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGcc                                                   >  2:3186090/1‑101 (MQ=255)
                                            gAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGccc                                                  >  1:3316367/1‑101 (MQ=255)
                                            gAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGccc                                                  <  2:4293437/101‑1 (MQ=255)
                                            gAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGccc                                                  >  2:3142051/1‑101 (MQ=255)
                                             aaGCCATTGCAGGAAATGCCCCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCa                                                 <  2:1942845/101‑1 (MQ=255)
                                             aaGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCa                                                 <  2:4348310/101‑1 (MQ=255)
                                              aGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAg                                                >  1:1610081/1‑101 (MQ=255)
                                              aGCCATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAg                                                <  2:3020086/101‑1 (MQ=255)
                                                ccATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAgcg                                              <  2:2352706/101‑1 (MQ=255)
                                                 cATTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAgcgc                                             >  2:3318132/1‑101 (MQ=255)
                                                  aTTGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGcc                                            <  1:3334948/101‑1 (MQ=255)
                                                    tGCAGGAAATGCACCCGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAg                                          >  2:327610/1‑101 (MQ=255)
                                                    tGCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTTGCCCAGCGcccg                                          >  1:2813766/1‑99 (MQ=255)
                                                     gCAGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAGt                                         <  1:2627804/101‑1 (MQ=255)
                                                       aGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAGttg                                       <  2:2149393/101‑2 (MQ=255)
                                                       aGGAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAGttg                                       <  1:2199628/101‑2 (MQ=255)
                                                        ggAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAGttga                                      <  2:2522454/101‑3 (MQ=255)
                                                        ggAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAGttga                                      <  2:2590500/101‑3 (MQ=255)
                                                         gAAATGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAGttgac                                     >  2:3203338/1‑98 (MQ=255)
                                                          aaaTGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAGttgacg                                    <  2:799017/101‑5 (MQ=255)
                                                          aaaTGCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAGttgacg                                    <  1:103774/101‑5 (MQ=255)
                                                              gCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAGttgacgcggt                                >  1:3628679/1‑93 (MQ=255)
                                                              gCACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAGttgacgcggt                                >  1:3715630/1‑93 (MQ=255)
                                                               cACCAGCCAGCGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAGttgacgcggtt                               <  1:1161473/101‑10 (MQ=255)
                                                                    gccagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTCTCGCCCAGCGCCAG.TTGACCCCGGtcggcc                         >  2:3362191/1‑96 (MQ=255)
                                                                     ccagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCa                       >  1:523276/1‑101 (MQ=255)
                                                                      cagcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCATCGCCAG.TTGAC.GCGGTTCGGTCAt                      >  1:2417208/1‑101 (MQ=255)
                                                                       agcGCGGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCTGTCAtt                     >  1:3937555/1‑101 (MQ=255)
                                                                         cgcgGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCa                   >  1:3282556/1‑101 (MQ=255)
                                                                         cgcgGC.GTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCa                   <  1:4362615/101‑1 (MQ=255)
                                                                           gcggcgTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCAc                  >  1:3983409/6‑101 (MQ=255)
                                                                           gcggcgTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCAc                  <  1:314291/96‑1 (MQ=255)
                                                                             ggcgTGATCGTGGG.CAGGAT.CGGTG.CCGTCTTCGTGGCATTAGC.AAGCTGGCGGGCGGTACTCTCGCCCAGCGTCAG.ATGAC.GCGGTTCGGTCATTCACGt                >  2:754088/4‑101 (MQ=255)
                                                                              gcgTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGtt               <  2:3844852/99‑1 (MQ=255)
                                                                               cgTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGGTCGGTCATTCACGTtc              >  1:3376673/2‑101 (MQ=255)
                                                                                gTGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCaagatcg             >  2:3486006/1‑95 (MQ=255)
                                                                                 tGATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTtctc            >  1:2999524/1‑101 (MQ=255)
                                                                                  gATCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTcc           >  1:161402/1‑101 (MQ=255)
                                                                                   aTCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCct          <  2:1446727/101‑1 (MQ=255)
                                                                                   aTCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCct          >  2:1170804/1‑101 (MQ=255)
                                                                                    tCGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCctt         <  2:460831/101‑1 (MQ=255)
                                                                                     cGTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCcttc        <  1:4024339/101‑1 (MQ=255)
                                                                                      gTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCcttct       <  1:3516164/101‑1 (MQ=255)
                                                                                      gTGGG.CAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCcttct       <  2:3881329/101‑1 (MQ=255)
                                                                                        tgggcAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCcttctt      >  2:1155723/5‑101 (MQ=255)
                                                                                        tgggcAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCcttctt      >  2:187327/5‑101 (MQ=255)
                                                                                         gggcAGGAT.CGGCG.CCGTCTTCTTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTg     <  1:779225/98‑1 (MQ=255)
                                                                                          ggcAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgg    >  2:1314265/3‑101 (MQ=255)
                                                                                            cAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgggt  <  2:1742608/101‑2 (MQ=255)
                                                                                            cAGGAT.CGGCG.CCGTCTTCGTGGCATTGGC.AAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAG.TTGAC.GCGGTTCGGTCATTCACGTTCTCCTTCTTgggt  >  1:1331540/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
GGCGTTCTTCACCGTGTTGAGCCGGTAGATCCCCGCCTCGACCGGAAGCCATTGCAGGAAATGCACCAGCCAGCGCGGCAGTGATCGTGGGCCAGGATACGGCGCCCGTCTTCGTGGCATTGGCAAAGCTGGCGGGCGGCACTGTCGCCCAGCGCCAGTTTGACACCGGTTCGGTCATTCACGTTCTCCTTCTTGGGG  >  NC_014034/2420141‑2420338

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG