Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,474,914	Δ1 bp	100%	intergenic (‑77/+49)	RCAP_rcc02301 ← / ← engA	hypothetical protein/GTP‑binding protein EngA

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,474,914	0	G→.	100.0%	106.9	34	intergenic (‑77/+49)	RCAP_rcc02301/engA	hypothetical protein/GTP‑binding protein EngA
Reads supporting (aligned to +/- strand):  new base (24/9):  ref base (0/1):  total (24/10)

CTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCGGGGGGGCGCAGCCCCCCGC.GGGTCGCCTCGGGCTTGCCCGAGGCGACATCAGCCCTTCGGTTTCGAGGGCAGGCCCTTG.TCCTTGCGTGCGG  >  NC_014034/2474815‑2475006                                                                                                    |                                                                                               cTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCgg                                                                                               <  2:2362221/101‑1 (MQ=255)  tCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGGGCggg                                                                                              >  1:3343655/1‑101 (MQ=255)   cccccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCgggg                                                                                             <  2:1040152/101‑1 (MQ=255)   cccccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCgggg                                                                                             <  1:2610003/101‑1 (MQ=255)      cggggATATTTCCCCCAAGGTGACAGGAAACCGCGCCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggc                                                                                          <  2:3889193/99‑2 (MQ=255)      ccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggc                                                                                          <  2:971758/101‑2 (MQ=255)      ccGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggc                                                                                          <  1:25044/101‑2 (MQ=255)        gggATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGAGGGGGGGGCggggggagc                                                                                        >  1:1075047/1‑98 (MQ=255)         ggATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggcgca                                                                                       <  2:681398/101‑5 (MQ=255)         ggATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCggggggcgca                                                                                       <  1:2987078/101‑5 (MQ=255)          gATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAg                                                                                     <  2:1985731/101‑1 (MQ=255)             atTTCCCCCAAGGTGGCAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGccc                                                                                  <  1:1739367/101‑1 (MQ=255)             atTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGccc                                                                                  >  1:2636161/1‑101 (MQ=255)              tttCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcccc                                                                                 <  2:4358255/101‑1 (MQ=255)               ttCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGccccc                                                                                <  1:3544738/101‑1 (MQ=255)               ttCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGccccc                                                                                <  2:1074959/101‑1 (MQ=255)               ttCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGACCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGccccc                                                                                <  2:395296/101‑1 (MQ=255)                tCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcccccc                                                                               <  2:404133/101‑1 (MQ=255)                tCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGcccccc                                                                               <  1:4213307/101‑1 (MQ=255)                tCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGccccac                                                                               <  1:497051/101‑3 (MQ=255)                cccccaAAGGTGACAGGAACCCGCGCCCCCCCCCCCCCGACCGGCCCGGTTTTTCCTTGGCAAAATACGCAGGGGGGGGGGTG.GGGGGGGCGCAGcccccc                                                                               <  2:863636/100‑1 (MQ=25)                      aaGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGC.gggg                                                                        >  2:691082/1‑100 (MQ=255)                                 aaaCCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGC.GGGTCGCCCCggggt                                                             >  2:2458213/1‑99 (MQ=255)                                 aaaCCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGC.GGGCGGCCCCggggt                                                             >  2:2926493/1‑99 (MQ=37)                                    ccGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGC.GGGGCGCCTCCGGCCTGc                                                          >  2:4185238/1‑101 (MQ=25)                                              ccccACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGC.GGGCCGCCTCGCGCCGGCCCCCGGCgcg                                                >  1:1007509/1‑99 (MQ=21)                                                      gAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGC.GGGGGGCCTCGGGGTTTCCCCCGGCGGGCTCAGccc                                        >  1:295534/1‑101 (MQ=18)                                                         cGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGC.GGGTCGCCTCGGGCTTGCCCGCGGCGGCATCAGCCCCTc                                     >  1:1085907/1‑101 (MQ=21)                                                         cGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGC.GGGGCGGGCCGGGGCTGCCCGCCGCGGCCGCAGCCCCTc                                     >  2:28160/1‑101 (MQ=16)                                                            cccGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGC.GGGGCGGCTCGGGGGTGGCCCCGGCGGCATCCGCCCTCCGGt                                  >  2:3391675/1‑101 (MQ=18)                                                            cccGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGC.GGGGCGCCCCCGGGTTGCCCGCGGGGGCAACAAcccgttggg                                  >  1:2092211/1‑95 (MQ=16)                                                               ggCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGCGGGGCCGCCCCGGGGTGGCCCGGGGGGCCATCGCCCCTCCgtgtt                                >  2:3751680/1‑97 (MQ=12)                                                               ggCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGC.GGGTCGCCCCGGGGTTGCCCGGGGGGGCCTCAGCCCCTCGGtttt                               >  2:2560350/1‑100 (MQ=21)                                                                  ttttCCTTGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGC.GGGTCGCCTCGGGCCTGCCCCCGGCGGCATCAGCCCCTCGGTTTCTAg                            >  2:1751685/1‑101 (MQ=18)                                                                         tGGCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGC.GGGGCGCCTCGGGCTTGCCCGCGGGGACATCAGCCCCTCCGTTTTGGGGGGAGGc                     >  1:4191069/1‑101 (MQ=18)                                                                          ggCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGC.GGGGCGCCCCGGGGTTGCCCGCGGCGACAGCACCCCTTCCGTTTCGAGGGCCGGcc                    >  2:2768918/1‑101 (MQ=21)                                                                           gCCAAATACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGC.GGGGCGCCCCGGGCGTGCCCCCGGGGGCAACAGCCCCTCGGGTTTGGggggggggcc                   >  2:3887592/1‑94 (MQ=12)                                                                                 tACGCAAGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGC.GGGGCGCCTCGGGCTTGCCCGGGGCGGCATCAGCCCCTCGGGTTCGAGGGCAGGGCCCTG.Tcc            >  2:1451799/1‑101 (MQ=18)                                                                                      aaGGCGGGGGGTGC.GGGGGGCGCAGCCCCCCGC.GGGACGCCTCGGGCTTTCCCGAGGCGACATCAGCCCTTCGGGTTAGAGGGCAGGCCCTTG.TCCCtgcg       >  1:562790/1‑101 (MQ=21)                                                                                        ggCGGGGGGTGC.GGGGGGCGCAGCCCCCCGC.GGGGCGCCTCGGGCTTTCCCGCGGGGCCATCAGCCCTTCGGTTTTGAGGGGAGGCCCTTG.TCCTtgcggg     >  1:2660315/1‑99 (MQ=21)                                                                                         gCGGGGGGTGC.GGGGGGCGCAGCCCACCGC.GGGTCGCCCCGGGGTTGCCCGGGGGGGCACAACCCCTTCGGGTTCGAGGGGAGGCCCTTG.TCCTtgcgtgc    >  1:553017/1‑101 (MQ=16)                                                                                           ggggggTGC.GGGGGGCGCAGCCCCCCGC.GGGTCGCCTCGGGCTTGCCCGAGGCGACATCAGCCCCTCGGTTTCGAGGGCAGGCCCTTG.TCCTTGCGTgcgg  >  1:3675892/1‑101 (MQ=255)                                                                                           ggggggTGC.GGGGGGCGCAGCCCCCCGC.GGGTCGCCCCGGGGTTGCCCCAGGCGCCACCAGCCCTTCGGGTTCCAGAGCAGGGCCATGTTCCTtgcggcgg   >  2:201284/1‑97 (MQ=9)                                                                                           ggggggTGC.GGGGGGCGCAGCCCCCCGC.GGGGCGCCCCGGGGTTGCCCCGGGCGGCATCAGCCCTTCGGTTTTGCGGGCCGGCCCTTG.TCCTTGCGTgcgg  >  2:1526615/1‑101 (MQ=21)                                                                                                    |                                                                                               CTCCCCCGGGATATTTCCCCCAAGGTGACAGGAAACCGCGGCCCCCCCCACCCGAACGGCCCGGCTTTTCCTTGGCCAAATACGCAAGGCGGGGGGTGCGGGGGGGCGCAGCCCCCCGC.GGGTCGCCTCGGGCTTGCCCGAGGCGACATCAGCCCTTCGGTTTCGAGGGCAGGCCCTTG.TCCTTGCGTGCGG  >  NC_014034/2474815‑2475006 Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG