Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,022 Δ1 bp 6.7% intergenic (+70/‑172) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0220C→. 6.7% 27.1 0.0 ‑0.0165intergenic (+70/‑172)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/7):  ref base (62/92):  total (66/99)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.79e-01

Only 100 of 178 total aligned reads displayed.

GGCGGGCGCGCGGCACGACAGGCCGAGTAA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGAAGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGG  >  NC_014034/2493923‑2494121
                                                                                                    |                                                                                                           
 gcgggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGcc                                                                                                            <  1:1811761/101‑1 (MQ=21)
  cgggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCt                                                                                                           <  2:1168021/101‑1 (MQ=21)
   gggcgCGCGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCtt                                                                                                          >  2:628191/1‑101 (MQ=21)
     gcgcgcGGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTc                                                                                                        <  1:2083954/101‑1 (MQ=21)
      ggcgcgGCGCGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                       <  2:3910641/100‑1 (MQ=18)
      cgcgcgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                       <  1:3510733/101‑1 (MQ=21)
      cgcgcgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                       <  1:2563115/101‑1 (MQ=21)
      cgcgcgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCa                                                                                                       <  2:171363/101‑1 (MQ=21)
        cgcgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATc                                                                                                     <  2:420997/101‑1 (MQ=21)
        cgcgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATc                                                                                                     >  1:1322135/1‑101 (MQ=21)
         gcgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCt                                                                                                    <  1:602055/101‑1 (MQ=21)
          cgGCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCtt                                                                                                   <  2:3551263/101‑1 (MQ=21)
           ggCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                                  <  2:4069210/101‑1 (MQ=21)
           ggCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                                  <  2:1014675/101‑1 (MQ=21)
           ggCACGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTg                                                                                                  <  2:2326949/101‑1 (MQ=21)
              aCGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGccc                                                                                               <  1:1075202/101‑1 (MQ=21)
              aCGACAGGCCGAGTAAGGGGGGGGCGCGGCCCCCCGCCCGCAAGCCGAACCCCCCCAGGATATTTGCAGCAAAACGAAAACGGAGACCTTTCAACTTGccc                                                                                               >  1:1666772/1‑101 (MQ=255)
               gggcAGGCCGAGTAAGGGGGGGGCGCGGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCg                                                                                              <  2:2607488/98‑1 (MQ=18)
               cGACAGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTATCATCTTGCCCg                                                                                              <  1:2350473/101‑1 (MQ=18)
                   aGGCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCGAAATGAAACCGGGTGCCTTTCATCTTGCCCGAAat                                                                                          >  1:3626024/1‑101 (MQ=18)
                   aGGCCCAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCACAAAat                                                                                          <  2:137179/101‑1 (MQ=18)
                    ggCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                         >  1:2020565/1‑101 (MQ=21)
                    ggCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                         <  2:1477498/101‑1 (MQ=21)
                    ggCCGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAata                                                                                         >  2:4043230/1‑101 (MQ=21)
                     atctaGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAatat                                                                                        <  1:4384874/97‑1 (MQ=18)
                      cgggtaagggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                                                       <  1:3026218/92‑1 (MQ=18)
                      ccGAGTAAGGGGGGGGCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATc                                                                                       <  1:1463652/101‑1 (MQ=21)
                          gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                                   <  1:2456452/96‑1 (MQ=255)
                           taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                                  <  1:72196/97‑1 (MQ=255)
                             aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                                >  1:281857/3‑101 (MQ=255)
                                ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                             <  2:2757045/101‑1 (MQ=255)
                                ggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTg                                                                             <  2:1735060/101‑1 (MQ=255)
                                 gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                            <  2:2241220/101‑1 (MQ=255)
                                  ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaa                                                                           <  1:2149874/101‑1 (MQ=255)
                                     gcgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGACATTTGCAGCAAGATGAAAACGGATGCCTTTCATCTTGCCCGAACTATACCGGGGGGGGAG.Tgg                                                                       >  1:883252/1‑101 (MQ=18)
                                      cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGc                                                                      <  1:2374334/101‑1 (MQ=255)
                                       gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                                   <  1:3097965/101‑1 (MQ=255)
                                          gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                               <  1:1081080/101‑3 (MQ=255)
                                           cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                              <  2:3332561/101‑4 (MQ=255)
                                            ccccG.TCCTCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATTCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                            <  1:1331605/101‑6 (MQ=18)
                                            ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                            >  1:1562174/1‑96 (MQ=255)
                                              cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                           >  1:3570867/5‑95 (MQ=255)
                                              cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                           <  1:769318/97‑7 (MQ=255)
                                                cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                         <  1:573662/99‑9 (MQ=255)
                                                cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                         >  1:3401873/3‑93 (MQ=255)
                                                 gtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagccagagg                                                        >  1:1827773/2‑92 (MQ=21)
                                                  tCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagaggg                                                       <  1:1724067/101‑11 (MQ=255)
                                                  tCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagaggg                                                       >  2:2511595/1‑91 (MQ=255)
                                                  tCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagccagaggg                                                       >  2:3007661/1‑91 (MQ=18)
                                                       aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGCa                                                 >  2:2302328/1‑101 (MQ=18)
                                                       aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAGAGGGGGGCa                                                 >  1:4364755/1‑101 (MQ=18)
                                                         gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGCagc                                               >  2:450441/1‑99 (MQ=18)
                                                              cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cc.cc                                       >  2:688702/1‑101 (MQ=17)
                                                               tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.GCCAG.AGGGGGGC.AGCG.cc.ccc                                      <  2:4142574/101‑1 (MQ=17)
                                                                ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cc.cccc                                     <  2:1952332/101‑2 (MQ=17)
                                                                 cccccGGGATATTTGCAGCAAGATGAAACCGGATCCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cc.cccct                                    <  1:19700/101‑3 (MQ=17)
                                                                  ccccGGGATATGTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cc.cccctg                                   <  2:1322038/101‑4 (MQ=17)
                                                                    ccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.cc.cccctgcc                                 <  2:2951645/101‑6 (MQ=17)
                                                                     cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGACC.CCCTGccc                                 >  2:1214419/1‑101 (MQ=17)
                                                                      gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CC.CCCTGcccc                                >  1:2784255/1‑101 (MQ=0)
                                                                      gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CC.CCCTGcccc                                <  1:2186554/101‑1 (MQ=0)
                                                                       ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGAGAA.GGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcg                               >  1:4009471/1‑101 (MQ=17)
                                                                        gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgc                              <  1:2793530/101‑2 (MQ=0)
                                                                        gATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcgg                              >  1:2613171/1‑101 (MQ=17)
                                                                         gtatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgcc                             <  2:1429237/100‑3 (MQ=2)
                                                                         atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgcc                             <  1:1155047/101‑3 (MQ=2)
                                                                          tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcggcg                            >  2:3118786/1‑101 (MQ=18)
                                                                          tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.A.GCCAGAGGGGGC.AGCGCCC.CCCTGCCCcggcgg                           >  2:225289/1‑101 (MQ=17)
                                                                           atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCcggcgg                           >  1:947985/1‑101 (MQ=17)
                                                                           atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAGAGGGGGGCAGCAC.CC.CCCTGCCCcggcgg                           >  1:501559/1‑101 (MQ=17)
                                                                           anNTGCNGCAAGATGAANGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgccgg                           <  2:1707762/101‑1 (MQ=2)
                                                                             ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGCAGCG.CC.CCCTGCCCcgccggat                         >  2:91130/1‑99 (MQ=2)
                                                                             ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTc                         <  2:3706895/101‑1 (MQ=17)
                                                                              tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.CCGCCCTGCCCCGGCGGTcc                        >  2:1376656/1‑101 (MQ=18)
                                                                               gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCg                       >  2:3410585/1‑101 (MQ=18)
                                                                                cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCCGCGGGCCgg                      >  2:3847046/1‑100 (MQ=14)
                                                                                 agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTg                     >  2:1075039/1‑101 (MQ=18)
                                                                                  gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTgg                    >  1:1966347/1‑101 (MQ=17)
                                                                                   caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggc                   >  2:503747/1‑101 (MQ=17)
                                                                                   caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggc                   >  1:3860326/1‑101 (MQ=18)
                                                                                   caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggc                   <  1:3185265/101‑1 (MQ=17)
                                                                                    aaGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggcg                  <  2:1110206/101‑1 (MQ=17)
                                                                                    aaGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggcg                  >  2:2922315/1‑101 (MQ=18)
                                                                                     aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggcgg                 <  2:1229934/101‑1 (MQ=17)
                                                                                        tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGgcgc              >  2:4269411/1‑101 (MQ=17)
                                                                                        tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCG.CCTCCCTGCCCCGGCGGTCCGTGGCGgggc              >  1:414463/1‑101 (MQ=17)
                                                                                          aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCt            <  1:4261496/101‑1 (MQ=17)
                                                                                           aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCtt           <  1:1249351/101‑1 (MQ=17)
                                                                                            aCCGGATGCCTTTCATCTTGCCCGAAATATCCTGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg          >  2:3138641/1‑101 (MQ=18)
                                                                                            aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg          <  1:2335769/101‑1 (MQ=17)
                                                                                              cGGATTCCTTTCATCTTGCCGGAAATACCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc        <  2:3625771/101‑1 (MQ=14)
                                                                                              cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc        >  2:1859906/1‑101 (MQ=18)
                                                                                              cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGAGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc        >  2:3217971/1‑101 (MQ=18)
                                                                                              cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.ACG.CCAGAGGGGGGC.CGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc        >  2:2470170/1‑101 (MQ=18)
                                                                                              cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGGGGCGGCGCCTTGAc        >  1:2467268/1‑101 (MQ=17)
                                                                                              cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAA.G.CCAGAGGGGGGC.CGCGCCC.CCCTGCCCCGGCGGCCCGGGGCGGGGCCTTgcc        >  1:3069832/1‑99 (MQ=12)
                                                                                                gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGGGCCTTGAcgg      >  1:1750029/1‑101 (MQ=17)
                                                                                                  tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGCACCGC.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc    <  1:2541303/101‑1 (MQ=18)
                                                                                                    ccTTTCATCTTGCCCGAAATAGCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcgg  <  2:2365113/101‑1 (MQ=17)
                                                                                                    ccTTTCATCTGGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC.GGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcgg  <  1:4447110/101‑1 (MQ=17)
                                                                                                    |                                                                                                           
GGCGGGCGCGCGGCACGACAGGCCGAGTAA.GGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGAAGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGG  >  NC_014034/2493923‑2494121

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG