Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,047 Δ1 bp 9.2% intergenic (+95/‑147) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0470G→. 9.2% 31.6 ‑1.5 ‑0.0131intergenic (+95/‑147)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/8):  ref base (64/55):  total (68/63)
Fisher's exact test strand distribution p-value = 2.30e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.98e-01

Only 100 of 141 total aligned reads displayed.

AGTAAGGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGA.AGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGC  >  NC_014034/2493948‑2494146
                                                                                                   |                                                                                                            
gtaaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCgg                                                                                                             <  1:2456452/96‑1 (MQ=255)
 taaggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggg                                                                                                            <  1:72196/97‑1 (MQ=255)
   aggggggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCggggg                                                                                                          >  1:281857/3‑101 (MQ=255)
       gggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGa                                                                                                      <  2:2241220/101‑1 (MQ=255)
        ggggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGaa                                                                                                     <  1:2149874/101‑1 (MQ=255)
         gggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.t                                                                                                   >  1:1378452/1‑101 (MQ=255)
          ggCGCTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.Tg                                                                                                  <  2:257003/101‑1 (MQ=255)
           gcgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGACATTTGCAGCAAGATGAAAACGGATGCCTTTCATCTTGCCCGAACTATACCGGGGGGGGAG.Tgg                                                                                                 >  1:883252/1‑101 (MQ=18)
             gcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..g                                                                                             <  1:3097965/101‑1 (MQ=255)
              cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                                                           <  2:1195484/101‑1 (MQ=255)
               tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                                                          <  1:3568460/101‑2 (MQ=255)
               tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                                                          <  1:2144125/101‑2 (MQ=255)
                gCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aag                                                                                         <  1:1081080/101‑3 (MQ=255)
                 cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCCGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGAGAA.TGGC..G.aagcc                                                                                       >  1:2946572/1‑97 (MQ=18)
                 cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..G.aagc                                                                                        >  2:3866505/1‑98 (MQ=18)
                  ccccG.TCCTCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATTCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                                                      <  1:1331605/101‑6 (MQ=18)
                  ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                                                      <  1:1897539/101‑6 (MQ=255)
                  ccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcca                                                                                      >  1:1562174/1‑96 (MQ=255)
                    cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                                                     <  1:769318/97‑7 (MQ=255)
                    cccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccag                                                                                     <  1:3438156/97‑7 (MQ=255)
                      cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                                                   <  1:573662/99‑9 (MQ=255)
                      cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                                                   >  1:3401873/3‑93 (MQ=255)
                        tCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagaggg                                                                                 >  2:2511595/1‑91 (MQ=255)
                             aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCa                                                                          >  2:2302328/1‑101 (MQ=18)
                             aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAG.AGGGGGGCa                                                                          >  1:4364755/1‑101 (MQ=18)
                              aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCag                                                                         <  2:4028881/101‑2 (MQ=18)
                               gCGGACTCCCCCCGGGATTTTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCagc                                                                        <  2:4145191/101‑3 (MQ=18)
                               gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCagc                                                                        >  2:450441/1‑99 (MQ=18)
                                 ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAG.AGGGGGGCagcgc                                                                      >  1:815323/1‑97 (MQ=17)
                                    cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.cc.cc                                                                >  2:688702/1‑101 (MQ=17)
                                     tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGCAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.cc.ccc                                                               >  1:2395499/1‑101 (MQ=17)
                                     tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.GCCAG..AGGGGGGC.AGCG.cc.ccc                                                               <  2:4142574/101‑1 (MQ=17)
                                      ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.cc.cccc                                                              <  2:1952332/101‑2 (MQ=17)
                                      ccccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.cc.cccc                                                              >  1:2365263/1‑100 (MQ=17)
                                       cccccGGGATATTTGCAGCAAGATGAAACCGGATCCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.cc.cccct                                                             <  1:19700/101‑3 (MQ=17)
                                        ccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAT.TGGC..GAA.G.CCGG.GGGGGGGCGGGCC.cc.ccccc                                                             >  2:3136668/1‑99 (MQ=17)
                                        ccccGGGATATGTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.cc.cccctg                                                            <  2:1322038/101‑4 (MQ=17)
                                          ccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.cc.cccctgcc                                                          <  2:2951645/101‑6 (MQ=17)
                                           cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGACC.CCCTGccc                                                          >  2:1214419/1‑101 (MQ=17)
                                           cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATACCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGccc                                                          >  1:2563268/1‑101 (MQ=17)
                                            gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CC.CCCTGcccc                                                         <  1:2186554/101‑1 (MQ=0)
                                            gggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CC.CCCTGcccc                                                         >  1:2784255/1‑101 (MQ=0)
                                            gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGcccc                                                         >  1:576951/1‑101 (MQ=17)
                                            gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGcccc                                                         <  1:3208022/101‑1 (MQ=17)
                                             ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CC.CCCTGCCCcg                                                        >  2:1447132/1‑101 (MQ=0)
                                             ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGAGAA.GGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCcg                                                        >  1:4009471/1‑101 (MQ=17)
                                             ggATANNTGCAGAAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCcg                                                        <  1:1594892/101‑1 (MQ=17)
                                              gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CC.CCCTGCCCcgc                                                       <  1:2793530/101‑2 (MQ=0)
                                              gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CC.CCCTGCCCcgc                                                       >  2:1683206/1‑100 (MQ=2)
                                               atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CC.CCCTGCCCcgcc                                                      <  1:1155047/101‑3 (MQ=2)
                                               atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CC.CCCTGCCCcgcc                                                      <  2:2819530/101‑3 (MQ=2)
                                                tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCcggcg                                                     >  2:3118786/1‑101 (MQ=18)
                                                tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCACcggcg                                                     >  2:341801/1‑101 (MQ=17)
                                                 atTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CC.CCCTGCCCcgccgg                                                    <  2:337533/101‑1 (MQ=2)
                                                 atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCcggcgg                                                    >  1:947985/1‑101 (MQ=17)
                                                 atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.A.GACAGAGGGGGGC.AGCGCCC.CCCTGCCCcggcgg                                                    >  1:477110/1‑101 (MQ=18)
                                                 anNTGCNGCAAGATGAANGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CC.CCCTGCCCcgccgg                                                    <  2:1707762/101‑1 (MQ=2)
                                                   ttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CC.CCCTGCCCcgccggat                                                  >  2:91130/1‑99 (MQ=2)
                                                   ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTc                                                  <  2:3706895/101‑1 (MQ=17)
                                                    tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.CCGCCCTGCCCCGGCGGTcc                                                 >  2:1376656/1‑101 (MQ=18)
                                                     gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCg                                                >  2:3410585/1‑101 (MQ=18)
                                                      cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCCGCGGGCCgg                                               >  2:3847046/1‑100 (MQ=14)
                                                       agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTg                                              >  2:1075039/1‑101 (MQ=18)
                                                        gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTgg                                             >  1:1966347/1‑101 (MQ=17)
                                                         caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggc                                            >  1:3860326/1‑101 (MQ=18)
                                                         caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggc                                            >  2:503747/1‑101 (MQ=17)
                                                          aaGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggcg                                           >  2:2922315/1‑101 (MQ=18)
                                                           aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTggcgg                                          <  2:1229934/101‑1 (MQ=17)
                                                              tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGgcgc                                       <  2:225445/101‑1 (MQ=17)
                                                              tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.CCTCCCTGCCCCGGCGGTCCGTGGCGgggc                                       >  1:414463/1‑101 (MQ=17)
                                                                 aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                    <  1:1249351/101‑1 (MQ=17)
                                                                  aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                   <  1:2335769/101‑1 (MQ=17)
                                                                   ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTgc                                  >  1:3157711/1‑100 (MQ=17)
                                                                    cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                 >  2:1859906/1‑101 (MQ=18)
                                                                    cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGAGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                 >  2:3217971/1‑101 (MQ=18)
                                                                    cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.ACG.CCAG.AGGGGGGC.CGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                 >  2:2470170/1‑101 (MQ=18)
                                                                    cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAA.G.CCAG.AGGGGGGC.CGCGCCC.CCCTGCCCCGGCGGCCCGGGGCGGGGCCTTgcc                                 >  1:3069832/1‑99 (MQ=12)
                                                                    cGGATGCCTTTCATCTTGCCCGAAATACCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                 <  1:1762593/101‑1 (MQ=17)
                                                                     ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGaag                                >  2:590309/1‑99 (MQ=17)
                                                                      gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGGGCCTTGAcgg                               >  1:1750029/1‑101 (MQ=17)
                                                                        tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCACCGC.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                             <  1:2541303/101‑1 (MQ=18)
                                                                          ccTTTCATCTTGCCCGAAATAGCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcgg                           <  2:2365113/101‑1 (MQ=17)
                                                                          ccTTTCATCTGGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.GGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcgg                           <  1:4447110/101‑1 (MQ=17)
                                                                            tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                         >  1:33748/1‑101 (MQ=18)
                                                                            tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                         <  2:2395349/101‑1 (MQ=17)
                                                                             ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggg                        >  2:109218/1‑101 (MQ=18)
                                                                              tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                       >  2:940965/1‑101 (MQ=18)
                                                                               cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGGGCCGCCTTGAGGGGCGGGGgcg                      >  2:1158134/1‑101 (MQ=17)
                                                                                aTCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcgc                     >  1:4316494/1‑101 (MQ=18)
                                                                                aTCTTGCCCGAAATATCCCGGGGGGTGAA.TAGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcgc                     <  1:621085/101‑1 (MQ=18)
                                                                                  cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAGGGGCGGGGGGGCgg                   >  1:2783674/1‑101 (MQ=17)
                                                                                  cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCgg                   <  2:1427906/101‑1 (MQ=18)
                                                                                     gCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggggatcggaa                >  2:3740502/1‑93 (MQ=17)
                                                                                     gCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.A.GCCA.GGGGGGGGCAGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCa                >  1:3528497/1‑101 (MQ=21)
                                                                                       ccGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt              >  2:2103695/1‑101 (MQ=18)
                                                                                        ggAAGTATCCGGGGGGGTGAA.TGGC..GAA.G.CCGG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa             <  1:1523195/100‑1 (MQ=21)
                                                                                               tcggggggggTGAA.TGGC..G.AGG.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa      <  1:3249841/97‑1 (MQ=25)
                                                                                                 ccGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt    <  2:3162691/101‑1 (MQ=21)
                                                                                                 ccGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAtt    >  2:1775408/1‑101 (MQ=21)
                                                                                                  cGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAACGAAAtt  >  2:683660/1‑100 (MQ=21)
                                                                                                   |                                                                                                            
AGTAAGGGGGGGCGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGA.AGGGGGGCAAGCG.CC.CCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGC  >  NC_014034/2493948‑2494146

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG