Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,566,598 Δ1 bp 2.6% intergenic (+70/+124) purD → / ← sdaA phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,566,5980A→. 2.6% 4.5 0.0 ‑0.2156intergenic (+70/+124)purD/sdaAphosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (70/82):  total (72/84)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.14e-01

Only 100 of 188 total aligned reads displayed.

CCGCGACATCGGCTGGCGCGCGCTCTGAGACGAAAC.GGGGGCGCTGCCCCC.CTCCTG..CTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCC  >  NC_014034/2566501‑2566698
                                                                                                      |                                                                                                    
ccgcGGCGTCGGCTGGCGCGCGCTGTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCCATTCCCCCCCCGGGATA.TTTCGGGCAAGATaa                                                                                                      <  2:2853815/101‑1 (MQ=18)
 cgcgACATCGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATaaa                                                                                                     <  1:2582465/101‑1 (MQ=21)
 cgcgACATCGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATaaa                                                                                                     <  2:3837105/101‑1 (MQ=21)
 cgcgACATCGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATaaa                                                                                                     <  2:1980484/101‑1 (MQ=21)
 cgcgACATCGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAAATaaa                                                                                                     <  1:295598/101‑1 (MQ=21)
  gcgACATCGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGTCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAaag                                                                                                    <  1:1358392/101‑1 (MQ=21)
    gACATCGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAaagaa                                                                                                  <  2:3920021/101‑1 (MQ=21)
    gACATCGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAaagaa                                                                                                  <  2:1061428/101‑1 (MQ=21)
    gACATCGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.ATTCGGGCAAGATAaagaa                                                                                                  >  1:2318559/1‑101 (MQ=21)
     aCATCGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAaaaaag                                                                                                 >  1:1271813/1‑101 (MQ=21)
      cATCGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAgg                                                                                                <  1:2985799/101‑1 (MQ=21)
      cATCGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAgg                                                                                                <  1:215566/101‑1 (MQ=21)
         cGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCt                                                                                             <  2:1414892/101‑1 (MQ=21)
          ggCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCtt                                                                                            <  2:1394035/101‑1 (MQ=21)
          ggCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCtt                                                                                            >  2:1299253/1‑101 (MQ=21)
          ggCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCtt                                                                                            <  2:3464254/101‑1 (MQ=21)
          gacTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCtt                                                                                            >  1:3966902/3‑101 (MQ=21)
            cTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCtttt                                                                                          <  1:4081669/101‑1 (MQ=21)
             tGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCCGGGTATTTTCGGGC.AGATAaaaaaagggttttt                                                                                         >  2:3027699/1‑92 (MQ=9)
               gcgcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCt                                                                                       <  1:4349531/101‑1 (MQ=21)
                cgcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                                                                      <  2:1094175/101‑1 (MQ=21)
                 gcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                                                                                     <  1:2359893/101‑1 (MQ=21)
                  cgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                                                                                    <  1:1807017/101‑1 (MQ=21)
                     gcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCCCCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATc                                                                                 <  1:817442/101‑1 (MQ=21)
                        ctGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCAAATTCGCCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTg                                                                              <  1:4265530/101‑1 (MQ=21)
                         tGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGc                                                                             <  2:1574485/101‑1 (MQ=21)
                          gagaCGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGcc                                                                            <  2:271613/101‑1 (MQ=21)
                          gagaCGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTGCCTTTCATCTTGcc                                                                            <  1:519025/101‑1 (MQ=21)
                           agaCGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGccc                                                                           <  2:4443182/101‑1 (MQ=21)
                            gaCGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCg                                                                          <  2:2243119/101‑1 (MQ=21)
                            gaCGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCg                                                                          >  1:1777787/1‑101 (MQ=21)
                              cGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaa                                                                        >  1:3643487/1‑101 (MQ=21)
                              cGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaa                                                                        <  1:740473/101‑1 (MQ=21)
                               gAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCCATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaaa                                                                       <  1:3107135/101‑1 (MQ=21)
                               gAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTTGGGGAAGATAAAGAAGGGCTTTTCCTTTTATCTTTCCCCaaa                                                                       >  1:3883112/1‑101 (MQ=17)
                               gAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaaa                                                                       <  2:4135017/101‑1 (MQ=21)
                                 aacggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAata                                                                     <  1:3403555/97‑1 (MQ=25)
                                 aacggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAata                                                                     >  1:2794101/5‑101 (MQ=25)
                                  acggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAatat                                                                    >  2:4193579/4‑101 (MQ=25)
                                  acggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAatat                                                                    <  1:1158219/98‑1 (MQ=25)
                                    agggggCGCTG.CCCC.CTCTTGGCCTTTGGCCAATTCA.CCCCTGGGATA.TTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCggggg                                                            >  1:3465033/2‑101 (MQ=0)
                                     gggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATccc                                                                 <  1:2729715/101‑1 (MQ=25)
                                      ggggCGCTGCCCCCTCTTGGC..CTTTGGCCAATTCA.CCCCTGGGATA.TTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATCTTGCCGCAAATATCCCgggggga                                                          <  2:2846502/101‑3 (MQ=2)
                                          cgcTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCCAAATATCCCggggg                                                            >  2:3331108/1‑101 (MQ=25)
                                           gcTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGa                                                           <  1:4128436/101‑1 (MQ=37)
                                            cTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATA.CTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAg                                                          >  2:3937016/1‑101 (MQ=37)
                                             tGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGAAA.TTTCGGGGCAGATAAAGAGGGTTTTTTCCTTTCTTCTTTCCCGGAATATCCCCCGGGacc                                                         >  1:192838/1‑99 (MQ=16)
                                             tGCCCCC.CTCTTGGCCTTCGGCCAACTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAAGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGc                                                         <  2:2564462/101‑1 (MQ=37)
                                              gCCCCC.CTCTTGGCCTTCGGCCAATTCCCCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGcc                                                        <  1:693658/101‑1 (MQ=38)
                                                   ccctcttggccTTCGGCCAATTCCCCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCt                                                    <  1:3591338/91‑1 (MQ=38)
                                                   ccctcttggccTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCt                                                    >  1:3981012/11‑101 (MQ=255)
                                                    cctcttggccTTCGTCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCtt                                                   <  2:3267489/92‑1 (MQ=37)
                                                    cctcttggccTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGCAATATCCCGGGGGAGCCCGCtt                                                   >  2:4177887/10‑101 (MQ=37)
                                                     ctcttggccTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTg                                                  <  1:281610/93‑1 (MQ=255)
                                                       cttggccTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcg                                                >  1:2922025/7‑101 (MQ=255)
                                                         tggccTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcggg                                              <  1:747769/97‑1 (MQ=255)
                                                         tggccTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcggg                                              >  1:2972992/5‑101 (MQ=255)
                                                          ggccTTCGGCCAATTCCCCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTgcgggc                                             <  1:3289530/98‑1 (MQ=255)
                                                             cTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGcgggcggg                                          >  2:2325259/1‑101 (MQ=255)
                                                                cGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGc                                       >  2:1158868/1‑101 (MQ=255)
                                                                    cAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAgcg                                   <  1:3361897/101‑1 (MQ=255)
                                                                     aaTTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAgcgc                                  >  1:1584280/1‑101 (MQ=255)
                                                                      aTTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcc                                 >  2:1839637/1‑101 (MQ=255)
                                                                        tCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcccc                               >  2:3359653/1‑101 (MQ=255)
                                                                         cgccccccGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGccccc                              <  1:1738269/99‑1 (MQ=255)
                                                                         cACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTTCCCGAAATATCCCGGGGGACCCCGCCTGCGGGCGGGGGCCGGGccccc                              >  1:209796/1‑101 (MQ=37)
                                                                         cACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGccccc                              <  2:2317730/101‑1 (MQ=255)
                                                                         cACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGccccc                              <  2:2392515/101‑1 (MQ=255)
                                                                          cccccccGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCg                             <  2:1374118/100‑1 (MQ=255)
                                                                            cccccGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGaa                           >  2:2669688/1‑101 (MQ=255)
                                                                              cccGGGATA.TTTCGGGCTAGATAAAGATGGTCTTTTCCTTTCATCTTGCCCAAAATATCCCGGGGGTTCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAgc                         <  2:3970547/101‑1 (MQ=38)
                                                                                 gggATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGcc                      <  2:4105696/101‑1 (MQ=255)
                                                                                   gATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGTACCCCGCTTTCGGGCGGGGGCAGCGCCCCCGAAGTGCCCg                    >  2:1263553/1‑101 (MQ=38)
                                                                                   gATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCg                    >  1:4395774/1‑101 (MQ=255)
                                                                                   gATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCg                    >  2:3995857/1‑101 (MQ=255)
                                                                                     ta.tTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAc                  >  2:1474047/1‑101 (MQ=255)
                                                                                      a.tTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcg                 >  2:3277120/1‑101 (MQ=255)
                                                                                      a.tTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcg                 >  2:2809734/1‑101 (MQ=255)
                                                                                         ttCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcg               >  1:2178200/1‑101 (MQ=255)
                                                                                          tCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcgc              >  1:3069158/1‑101 (MQ=255)
                                                                                           cGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgcgcg             >  1:1274712/1‑101 (MQ=255)
                                                                                             ggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggc           <  2:3161282/101‑1 (MQ=255)
                                                                                             ggCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGAGCCCGACGCgcggc           >  1:3119114/1‑101 (MQ=255)
                                                                                             ggCAAAATAAACAAGGTCTTTTCCTTTCATCTTTCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggc           <  1:3299003/101‑1 (MQ=255)
                                                                                              gCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggcg          >  1:1149322/1‑101 (MQ=255)
                                                                                               cAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGcggcgg         >  2:853590/1‑101 (MQ=255)
                                                                                               cAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGcggcgg         >  2:3375116/1‑101 (MQ=255)
                                                                                               cAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGcggcgg         >  1:2476696/1‑101 (MQ=255)
                                                                                                naGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgnnnngnn        >  1:612353/2‑99 (MQ=255)
                                                                                                  gATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCTCCCGGCGCGCGGCGGGCt      <  2:401558/101‑1 (MQ=255)
                                                                                                   aTAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt     <  1:996566/101‑1 (MQ=255)
                                                                                                    tAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCttc    >  1:3744093/1‑100 (MQ=255)
                                                                                                    tAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtcg    >  1:1054335/1‑99 (MQ=255)
                                                                                                     aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGGTTgc   >  1:160862/1‑101 (MQ=255)
                                                                                                     aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc   >  2:1166172/1‑101 (MQ=255)
                                                                                                     aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc   >  1:1732047/1‑101 (MQ=255)
                                                                                                     aaaGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc   >  2:55519/1‑101 (MQ=255)
                                                                                                      aagaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGGTTgcc  >  1:2823153/1‑101 (MQ=255)
                                                                                                      aagaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcc  >  2:1850171/1‑101 (MQ=255)
                                                                                                      aagaagGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgcc  >  2:1433106/1‑101 (MQ=255)
                                                                                                      |                                                                                                    
CCGCGACATCGGCTGGCGCGCGCTCTGAGACGAAAC.GGGGGCGCTGCCCCC.CTCCTG..CTTCGGCCAATTCACCCCCCGGGATA.TTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGCC  >  NC_014034/2566501‑2566698

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG