Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,617,778 Δ1 bp 100% intergenic (‑152/‑462) RCAP_rcc02439 ← / → RCAP_rcc02440 hypothetical protein/AsnC/Lrp family transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,617,7780C→.100.0% 22.5 166intergenic (‑152/‑462)RCAP_rcc02439/RCAP_rcc02440hypothetical protein/AsnC/Lrp family transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (89/67):  ref base (0/10):  total (89/77)
Fisher's exact test strand distribution p-value = 3.30e-04
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.11e-08

Only 100 of 221 total aligned reads displayed.

GCGGGGATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTCCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCTGCAGA  >  NC_014034/2617678‑2617878
                                                                                                    |                                                                                                    
 cggggATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTcc                                                                                                     >  2:10278/1‑101 (MQ=255)
 cggggATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTcc                                                                                                     <  1:2165129/101‑1 (MQ=255)
  ggggATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccc                                                                                                    <  2:2067071/101‑1 (MQ=255)
       tCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTcccccccc                                                                                               >  2:1197961/1‑101 (MQ=255)
       tCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTcccccccc                                                                                               <  2:1896603/101‑1 (MQ=255)
       tCAAGGCCGGAATTCGGACAGCCGACACAGAGCGGCTGACAGCTGCCGGCGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCCcccccctc                                                                                               <  2:3673053/101‑3 (MQ=255)
       ccAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTcccccccc                                                                                               <  1:2666850/100‑1 (MQ=255)
        cAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccg                                                                                              >  1:602566/1‑100 (MQ=255)
        cAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccc                                                                                              >  2:934268/1‑101 (MQ=255)
         aaGGCCGGAATTCGGGCCGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccgg                                                                                             <  1:2647845/101‑3 (MQ=255)
          aGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccggc                                                                                            >  1:956783/1‑98 (MQ=255)
          aGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccggc                                                                                            >  2:1246822/1‑98 (MQ=255)
          aGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccggc                                                                                            <  1:353662/101‑4 (MQ=255)
           ggCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTccccccccggcc                                                                                           >  1:698393/1‑97 (MQ=255)
            gCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCa                                                                                         <  2:701873/101‑1 (MQ=255)
             ccGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCaa                                                                                        >  1:1950036/1‑101 (MQ=255)
             ccGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCaa                                                                                        <  2:2479278/101‑1 (MQ=255)
              cGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCaaa                                                                                       <  1:175323/101‑1 (MQ=255)
              cGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCaaa                                                                                       <  2:2412020/101‑1 (MQ=255)
                taaTTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGc                                                                                     <  2:2357000/100‑1 (MQ=255)
                gAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGc                                                                                     <  2:3283749/101‑1 (MQ=255)
                gAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGc                                                                                     >  1:3886435/1‑101 (MQ=255)
                 aaTTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCCAAGca                                                                                    >  1:2357150/1‑100 (MQ=255)
                   ttCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGcccc                                                                                  <  1:162210/101‑1 (MQ=255)
                    tCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCg                                                                                 <  2:3780409/101‑1 (MQ=255)
                        gCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGccc                                                                             <  2:628644/101‑1 (MQ=255)
                        gCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGccc                                                                             >  2:3784605/1‑101 (MQ=255)
                         cggCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGACTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCg                                                                            <  2:2966582/99‑1 (MQ=255)
                            ccGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACt                                                                         <  1:961510/101‑1 (MQ=255)
                             cGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTc                                                                        <  1:3247335/101‑1 (MQ=255)
                             cGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTc                                                                        <  1:2687442/101‑1 (MQ=255)
                              gACACAGCGCAGCGGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCg                                                                       <  1:1246915/101‑1 (MQ=255)
                               acacAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGt                                                                      <  2:202988/101‑1 (MQ=255)
                                 acaGGGCAGCTGACAGCCGCCGAGGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCCCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCt                                                                    <  1:1003208/101‑1 (MQ=255)
                                 acaGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCt                                                                    >  2:1392207/1‑101 (MQ=255)
                                 acaGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCt                                                                    <  1:2332255/101‑1 (MQ=255)
                                     cgcAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTcg                                                                >  2:289816/1‑101 (MQ=255)
                                     cgcAGCTGACAGCCGCCGACGATGTCGAAAGGCAGCGTGGACAGCGACAGCGGCCTCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTcg                                                                <  1:3015258/101‑1 (MQ=255)
                                      gcAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTcgc                                                               >  2:3030928/1‑101 (MQ=255)
                                      gcAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTcgc                                                               >  1:643039/1‑101 (MQ=255)
                                      gcAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTcgc                                                               >  1:3759912/1‑101 (MQ=255)
                                       cAGCTGCAAGCCGCCGACGATGTCGAAATGCAGCGGGGACAGCGACAGCGGCGTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTcgcg                                                              <  1:55116/101‑1 (MQ=255)
                                        cgCTGACAGCCGCCGACGATGTCGAAATGCAGCGGGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCgcgg                                                             <  2:2183095/100‑1 (MQ=255)
                                        aGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCCTCTGTCgcgg                                                             >  2:3989496/1‑101 (MQ=255)
                                          cTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCgcggcg                                                           <  1:4243665/101‑1 (MQ=255)
                                           tGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGa                                                          <  2:880403/101‑1 (MQ=255)
                                             aaggccgccGACGAAGTCAAAATGCAGCGGGGACAGCGCCACCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACg                                                        <  2:1458783/98‑1 (MQ=255)
                                              cAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACgg                                                       <  2:601712/101‑1 (MQ=255)
                                                gccgccGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAACCCCCGGCCCGACTCGTCTGTCGCGGCGACGGAt                                                     >  1:3819590/1‑101 (MQ=255)
                                                   gccgACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGAtgtg                                                  <  1:1392304/101‑1 (MQ=255)
                                                     cgacgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTc                                                >  2:1694120/1‑101 (MQ=255)
                                                     cgacgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTc                                                >  1:2581823/1‑101 (MQ=255)
                                                     cgacgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTc                                                <  2:3717869/101‑1 (MQ=255)
                                                      gacgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCa                                               <  1:4290042/101‑1 (MQ=255)
                                                      gacgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCa                                               >  2:3513144/1‑101 (MQ=255)
                                                       gcgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCAt                                              <  2:2570664/100‑1 (MQ=255)
                                                        cgaTGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATg                                             >  2:1115891/1‑101 (MQ=255)
                                                           tGTCGAAATGCAGCGTGGACAGCGACAGCGCCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGc                                          <  1:876973/101‑1 (MQ=255)
                                                              cGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCttt                                       >  1:3591823/1‑101 (MQ=255)
                                                              cGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCttt                                       <  1:2727209/101‑1 (MQ=255)
                                                               gAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTc                                      <  2:3819337/101‑1 (MQ=255)
                                                                aaaTGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCg                                     >  2:3529537/1‑101 (MQ=255)
                                                                 aaTGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGc                                    <  2:2655291/101‑1 (MQ=255)
                                                                  aTGCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCa                                   <  2:502683/101‑1 (MQ=255)
                                                                    gCAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGc                                 <  1:859280/101‑1 (MQ=255)
                                                                     cAGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGcc                                >  2:3647973/1‑101 (MQ=255)
                                                                      aGCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCt                               >  1:2041134/1‑101 (MQ=255)
                                                                       gCGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTc                              <  2:1853211/101‑1 (MQ=255)
                                                                        cGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGGCGGATGTGTCATGGGGTTTCGCAGCCCcc                             >  1:1267334/1‑101 (MQ=255)
                                                                        cGTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGAGGTGTCATGGGCTTTCGCAGCCTcc                             >  1:1994395/1‑101 (MQ=255)
                                                                         gTGGACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTccc                            >  1:3119910/1‑101 (MQ=255)
                                                                         gTGGACAGCGACAGCGGCCTCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTccc                            <  1:3092750/101‑1 (MQ=255)
                                                                          tGGACAGCGACAGCGGCCTCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCg                           <  1:3194190/101‑1 (MQ=255)
                                                                           ggACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGa                          >  2:2146064/1‑101 (MQ=255)
                                                                           ggACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGa                          <  2:1538856/101‑1 (MQ=255)
                                                                           ggACAGCGACAGCGGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGGGTCATGGGCTTTCGGAGCCTCCCGa                          >  2:3829730/1‑101 (MQ=255)
                                                                             acagcgacagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGAAGGAAGTGTCATGGGCTTTCGCAGCCTCCCGAAg                        >  1:1486073/1‑101 (MQ=255)
                                                                             acagcgacagcgCCCTCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAg                        <  1:1536401/101‑1 (MQ=255)
                                                                               agcgacagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGtt                      <  1:1403366/101‑1 (MQ=255)
                                                                               agcgacagcgGCCTCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGtt                      <  1:3337951/101‑1 (MQ=255)
                                                                                  gacagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTgcg                   >  1:484882/1‑101 (MQ=255)
                                                                                  gacagcgGCCTCCCGGCT.CCCCCCCCGGCCAAACCCCCGGCCCGACCCGTCTGTCCAGCCGACGGACGCTACAGGGGCTTTCGCAGCCTCCCgcaaattcg                   >  1:1606356/1‑93 (MQ=255)
                                                                                   acagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGGGACGGATGTGTGATGGGCTTTCGCCGCCCTCCGCAGTTgcgc                  >  1:4120874/1‑101 (MQ=255)
                                                                                   acagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGCAGTTgcgc                  >  2:961384/1‑101 (MQ=255)
                                                                                    cagcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCGCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCa                 >  1:2203436/1‑101 (MQ=255)
                                                                                     agcgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTGGCGCaa                >  2:1015086/1‑101 (MQ=255)
                                                                                       cgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAAcc              >  1:2608488/1‑101 (MQ=255)
                                                                                       cgGCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAAcc              <  1:1874507/101‑1 (MQ=255)
                                                                                        ggCCTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAAccc             <  2:1645805/101‑1 (MQ=255)
                                                                                        ggCCCCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAAccc             <  1:1198052/101‑1 (MQ=255)
                                                                                        gcccTCCCGGC.CCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAAccc             <  2:1959826/99‑1 (MQ=255)
                                                                                          ccccccGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCcgc           <  1:252208/98‑1 (MQ=255)
                                                                                          ccTCCCGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCcgc           >  2:843367/1‑101 (MQ=255)
                                                                                             cccGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGc        >  1:3621277/1‑101 (MQ=255)
                                                                                              ccGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCt       >  1:2382227/1‑101 (MQ=255)
                                                                                               cGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCCTGGGCTTTCGCAGCCTCCCGAAGTTGCGCCACCCGCGGCTg      >  1:1678731/1‑101 (MQ=255)
                                                                                               cGGCT.CCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCgggcg      >  2:4239103/1‑98 (MQ=255)
                                                                                                   ccccccccccGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCTGCAg   <  1:1440255/100‑1 (MQ=255)
                                                                                                    cccccccccGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCTGCAGa  <  1:3730809/101‑1 (MQ=255)
                                                                                                    cccccccccGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCTGCAGa  <  1:1137069/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
GCGGGGATCAAGGCCGGAATTCGGGCAGCCGACACAGCGCAGCTGACAGCCGCCGACGATGTCGAAATGCAGCGTGGACAGCGACAGCGGCCTCCCGGCTCCCCCCCCCGGCCAAAGCCCCGGCCCGACTCGTCTGTCGCGGCGACGGATGTGTCATGGGCTTTCGCAGCCTCCCGAAGTTGCGCAACCCGCGGCTGCAGA  >  NC_014034/2617678‑2617878

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG