Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,254,687 Δ1 bp 100% intergenic (+12/‑171) glpX → / → recJ fructose‑1,6‑bisphosphatase/single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,254,6870G→.100.0% 55.4 100intergenic (+12/‑171)glpX/recJfructose‑1,6‑bisphosphatase/single‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (1/81):  ref base (16/0):  total (19/81)
Fisher's exact test strand distribution p-value = 1.79e-17
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.05e-09

Only 100 of 145 total aligned reads displayed.

ACCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.CCGGGGGGTATGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTT  >  NC_014034/3254587‑3254779
                                                                                                     |                                                                                              
 cccGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgg                                                                                               >  2:4195548/1‑101 (MQ=255)
 cccGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgg                                                                                               >  1:4008861/1‑101 (MQ=255)
 cccGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGCGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgc                                                                                               >  1:1369077/1‑100 (MQ=255)
  ccGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACggg                                                                                              >  1:629622/1‑101 (MQ=255)
  ccGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACggg                                                                                              <  1:151967/101‑1 (MQ=255)
   cGAAATTGATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACcggg                                                                                             >  2:614764/1‑101 (MQ=255)
   cGAAATTCATCGAGGCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggg                                                                                             <  2:1488985/101‑1 (MQ=255)
   cGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggg                                                                                             <  1:1442014/101‑1 (MQ=255)
   cGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggg                                                                                             <  2:3006413/101‑1 (MQ=255)
    gAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgaggg                                                                                            >  2:2018056/1‑101 (MQ=255)
     aaaTTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggg                                                                                           >  2:1582706/1‑101 (MQ=255)
     aaaTTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACggggga                                                                                           <  2:2907575/101‑2 (MQ=255)
       aTTCATCGAGACCTAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCCCAACCCGATCCGCTGAGGCAACGCT.ACgggggact                                                                                         <  1:3523082/101‑4 (MQ=255)
       aTTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggact                                                                                         <  2:1834448/101‑4 (MQ=255)
       aTTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggact                                                                                         <  2:2675062/101‑4 (MQ=255)
         tCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggactgc                                                                                       <  1:102378/101‑6 (MQ=255)
         tCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggactgc                                                                                       <  1:1905845/101‑6 (MQ=255)
          cATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCTACCggggggcgcc                                                                                       >  1:290178/1‑97 (MQ=255)
           aTCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggactgccg                                                                                     <  2:4393354/101‑8 (MQ=255)
             cGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCTACCggggggggcgggc                                                                                    >  1:1531343/1‑94 (MQ=255)
             cGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACtgggggggggccgc                                                                                   >  2:2216790/1‑93 (MQ=255)
                gaCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCTACCggggggggggcggcgc                                                                                 >  2:30214/1‑91 (MQ=255)
                 aCCGAAACGATCCTGATTCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.Tccccc                                                                             <  2:4008605/101‑1 (MQ=255)
                  ccGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACCGGGGGGGGGCCG.Cccccc                                                                             >  2:3575043/1‑101 (MQ=255)
                  ccGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCg                                                                            <  2:2089359/101‑1 (MQ=255)
                  ccGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCg                                                                            <  1:2162111/101‑1 (MQ=255)
                       aCGAGCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCAccc                                                                       <  2:3025804/101‑1 (MQ=255)
                         gATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCC.GCCCCCCCCccccc                                                                     >  2:2765814/1‑101 (MQ=255)
                          aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACCGGGGGCGGGCGG.GCCCCCCCCccccc                                                                     >  1:63900/1‑101 (MQ=255)
                          aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCt                                                                    <  2:1941540/101‑1 (MQ=255)
                          aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCt                                                                    <  1:207301/101‑1 (MQ=255)
                          aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCt                                                                    <  2:470317/101‑1 (MQ=255)
                          aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCt                                                                    <  1:2852981/101‑1 (MQ=255)
                          aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCt                                                                    <  2:756433/101‑1 (MQ=255)
                            ccTGATGCGCTCGACAACCGGCTCGGTGCGCCGTGTGCTCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCCCCCCCTgc                                                                  <  2:2965548/101‑1 (MQ=255)
                            ccTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTgc                                                                  <  1:91591/101‑1 (MQ=255)
                               gATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTgttcg                                                               <  2:3053207/101‑5 (MQ=255)
                               gATGCGCTCGAAAACCGGCTCGGTGCACCGTGTGATCTATCGCACCCCGATCCGCTGAGGCACCGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGcgcg                                                               <  2:3388837/101‑1 (MQ=255)
                                aTGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGt                                                              <  1:3087624/101‑1 (MQ=255)
                                  gcgcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.t                                                           <  2:1628371/101‑1 (MQ=255)
                                   cgcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.tt                                                          <  2:1805234/101‑1 (MQ=255)
                                      tCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTg                                                       <  1:1618326/101‑1 (MQ=255)
                                         aaaaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGTTGCCC.TCCCCC.CACCCCCCCcgcgccattttttc                                                     >  2:3394984/1‑91 (MQ=255)
                                          aaaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCaa                                                   <  2:2089945/101‑1 (MQ=255)
                                           aaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCGC.GCCCCCCCGCCCCCCGCGCGCA.ttttttccag                                                  >  2:470286/1‑95 (MQ=255)
                                           aaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAg                                                  <  1:333216/101‑1 (MQ=255)
                                            aCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAgg                                                 <  1:30227/101‑1 (MQ=255)
                                            aCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAgg                                                 <  2:1709448/101‑1 (MQ=255)
                                              cGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGaa                                               <  2:868916/101‑1 (MQ=38)
                                              cGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGaa                                               <  1:2694765/101‑1 (MQ=38)
                                              cGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGaa                                               <  1:3917274/101‑1 (MQ=38)
                                               ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGaaa                                              <  1:2750979/101‑1 (MQ=38)
                                                gCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGaaaa                                             <  1:1677979/101‑1 (MQ=38)
                                                 cccGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAAc                                            <  1:614832/99‑1 (MQ=38)
                                                 cTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCG.GCCCCCGCCCCCCCCGCGCGGA.TTTTTCCAAGGaaaaa                                            >  2:4141532/1‑100 (MQ=255)
                                                 cTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAAc                                            <  2:3258352/101‑1 (MQ=38)
                                                   cGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACgg                                          <  1:3682548/101‑1 (MQ=38)
                                                     gTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGCTGGCCGCCCCCCCCCCCCCCCCGCGGGT..TTTTCCAAAGCAAAACggg                                         >  2:4134192/1‑101 (MQ=255)
                                                     gTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACCGGGGGGGGGCCG.CCCCCCCCCCCCCCCCCGCGCT.TTTTTCCAAGGAAAACggg                                         >  1:1271062/1‑101 (MQ=255)
                                                      tGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.GTGCCC.TCCCCCCCCCCCCCCCCCCGGATTTTTTCCAAGAAAAAAgggg                                        >  2:3584618/1‑101 (MQ=255)
                                                      tGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGc                                       <  2:1972154/101‑1 (MQ=38)
                                                          ccGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGtt                                   <  2:2819903/101‑1 (MQ=38)
                                                           cGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGttt                                  <  2:3548507/101‑1 (MQ=38)
                                                            gggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTc                                 <  2:229552/101‑1 (MQ=38)
                                                             ggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCa                                <  2:1162709/101‑1 (MQ=38)
                                                               tGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCAcc                              <  2:1273527/101‑1 (MQ=38)
                                                                gATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCt                             <  2:629554/101‑1 (MQ=38)
                                                                gATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCt                             <  2:1097692/101‑1 (MQ=38)
                                                                gATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCt                             <  1:1389605/101‑1 (MQ=38)
                                                                gATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCt                             <  2:3744515/101‑1 (MQ=38)
                                                                 aTCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCtt                            <  2:1502511/101‑1 (MQ=38)
                                                                 aTCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCtt                            <  1:3572761/101‑1 (MQ=38)
                                                                  tCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCTTg                           <  2:3633531/101‑1 (MQ=38)
                                                                  tCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCTTg                           <  2:2026918/101‑1 (MQ=38)
                                                                    tATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGGCG.TCCCCCGCACCCCCCCCGCGGA.TTTTTCCCAGGAAAAAGGGGGCGGTTCACCTTGGc                         >  2:2421992/1‑101 (MQ=16)
                                                                    tATCGAAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGCA.CACG.CCCCCCCCCCCCCCTGCCCCGG.TTTTGCCCACGAAAAAAGGGGCGGTTCAACTTTGc                         >  2:736775/1‑101 (MQ=255)
                                                                     aTCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.GTGCCG.CCCCCCCCCCCCCCCCCCCGTA.TTTTTCCCAAGAAAAAGGGGCCGCTTTAcccctgcc                        >  1:781342/1‑95 (MQ=255)
                                                                     aTCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGcc                        <  2:873490/101‑1 (MQ=38)
                                                                      tCGCAACCCGCTCCGGTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCg                       <  2:1829969/101‑1 (MQ=37)
                                                                      tCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCg                       <  1:1852414/101‑1 (MQ=38)
                                                                      tCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCg                       <  2:3718844/101‑1 (MQ=38)
                                                                      tCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCg                       <  1:3327262/101‑1 (MQ=38)
                                                                       cGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGGGGCCG.CCCCCCCCCCCCCCCCCCCCCC.TTTTTCTAAGAAAAAAGGGG.CGGTTCACCCTTGCCgc                      >  2:1708463/1‑100 (MQ=255)
                                                                       cGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGa                      <  2:3599946/101‑1 (MQ=38)
                                                                         cAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGCGGCCG.CCCCCCCCCCCCCCCCCCCGCT.TTTTTCCACGAAAAAAGGGGCGGGTTTTCCTTGGCCGaa                     >  2:2482409/1‑101 (MQ=255)
                                                                          accccGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAat                   <  1:892508/99‑1 (MQ=38)
                                                                           aCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAata                  <  2:416097/101‑1 (MQ=38)
                                                                             ccGATCCGCTGAGGCAACGCT.ACGGGGGG.GTGCCC.GCCCCCCCCCCCCCCCCGCGGA.TTTTTCCCAGGAAAAAAGGGGCGGTTTACCCTTGCCGcaaaaac                >  2:3968214/1‑97 (MQ=255)
                                                                               gATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATccc              <  1:2216929/101‑1 (MQ=38)
                                                                                 tCCGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCgg            <  1:2646192/101‑1 (MQ=38)
                                                                                  ccGCTGAGGCAACGCT.ACGGGGGG.CTGCCG.TCCCCCCCCCCCCCCGCGCGTA.TTTTTCCAAGGAAAACGGGGGCGGTTTCCCTTTGCCGCAAAATCCCcgg           >  2:2798651/1‑101 (MQ=255)
                                                                                  ccGCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCggg           <  2:2744704/101‑1 (MQ=38)
                                                                                    gCTGAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCggggg         <  1:2078631/101‑1 (MQ=38)
                                                                                       gAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGa      <  1:470338/101‑1 (MQ=38)
                                                                                       gAGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGa      <  2:3481536/101‑1 (MQ=38)
                                                                                        aGGCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGaa     <  1:2420814/101‑1 (MQ=38)
                                                                                         ggCAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAAt    <  2:3635245/101‑1 (MQ=38)
                                                                                         ggCAACGCT.AC.GGCGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAAt    <  1:3833109/101‑1 (MQ=38)
                                                                                           cAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAAttt  <  1:2972945/101‑1 (MQ=38)
                                                                                           cAACGCT.AC.GGGGGACTGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAAttt  <  1:17647/101‑1 (MQ=38)
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ACCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.CCGGGGGGTATGCCG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTT  >  NC_014034/3254587‑3254779

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG