BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,461,372	Δ1 bp	65.3%	intergenic (‑82/+48)	RCAP_rcc03260 ← / ← RCAP_rcc03261	NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,461,372	0	G→.	65.3%	189.9 ‑0.1 ‑0.3	144	intergenic (‑82/+48)	RCAP_rcc03260/RCAP_rcc03261	NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein
Reads supporting (aligned to +/- strand): new base (49/45): ref base (25/25): total (74/70)
Fisher's exact test strand distribution p-value = 8.62e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.81e-01

Only 100 of 175 total aligned reads displayed.


GGAATTTCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.G.G.GG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGC  >  NC_014034/3461272‑3461461


ggAATTTCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGTGCGGCCAGACGCAGTTTGGCGCAGGGCCAaaggaag                                                                                                <  1:3699510/101‑1 (MQ=255)


 gAATTTCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAaaggaag.g                                                                                              >  1:1095397/1‑101 (MQ=255)


  aaTTTCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAg.g.g                                                                                            >  2:1442170/1‑101 (MQ=255)


    tttCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAg.g.g.gg                                                                                         >  1:2304594/1‑101 (MQ=255)


    tttCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAg.g.g.gg                                                                                         <  1:3263265/101‑1 (MQ=255)


     ttCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAg.g.g.ggc                                                                                        >  2:1073139/1‑100 (MQ=255)


     ttCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAg.g.g.ggc                                                                                        <  1:39818/101‑2 (MQ=255)


     ttCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAg.g.g.ggc                                                                                        >  2:3951635/1‑100 (MQ=255)


      tCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAg.g.g.ggcg                                                                                       >  1:2943815/1‑99 (MQ=255)


      tCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAg.g.g.ggcg                                                                                       >  2:1558446/1‑99 (MQ=255)


        aGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAg.g.g.ggcgca                                                                                     <  2:682441/101‑5 (MQ=255)


        aGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAg.g.g.ggcgca                                                                                     <  2:2168778/101‑5 (MQ=255)


        aGATCATTCATCGCAAGGCCCCCGTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAg.g.g.ggcgca                                                                                     <  2:1095309/101‑5 (MQ=255)


         gATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAg.g.g.ggcgcag                                                                                    <  1:2866097/101‑6 (MQ=255)


            cTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.G.G.GGCGCAGcca                                                                                 <  2:2391141/101‑2 (MQ=255)


            cTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.G.G.GGCGCAGcca                                                                                 <  1:2659292/101‑2 (MQ=255)


             tttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.G.G.GGCGCAGccac                                                                                <  1:4034899/101‑3 (MQ=255)


             tttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.G.G.GGCGCAGccac                                                                                <  2:1503820/101‑3 (MQ=255)


             tttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.G.G.GGCGCAGccac                                                                                >  2:512551/1‑99 (MQ=255)


              ttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGTAG.G.G.GGCGCAGccacc                                                                               <  2:2592178/101‑4 (MQ=255)


              ttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.G.G.GGCGCAGccacc                                                                               >  1:3579669/1‑98 (MQ=255)


              ttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.G.G.GGCGCAGccacc                                                                               <  2:700507/101‑4 (MQ=255)


               tCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.G.G.GGCGCAGccaccc                                                                              <  1:2965627/101‑5 (MQ=255)


                cATCGCAAGGCCCCCTTTTCGCGCCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.G.G.GGCGCAGccacccg                                                                             <  2:520795/101‑6 (MQ=255)


                cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGTG.G.GC.GCAGccacccg                                                                             <  1:3749461/101‑6 (MQ=255)


                cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.G.G.GGCGCAGccacccg                                                                             <  1:3106293/101‑6 (MQ=255)


                 aTCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.G.G.GGCGCAGccacccgg                                                                            >  1:3999888/1‑95 (MQ=255)


                 aTCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.G.G.GGCGCAGccacccgg                                                                            <  1:4169137/101‑7 (MQ=255)


                 aTCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGCTTGGCGCAGGGCCAAAGGAAG.G.GCGC.GCAGccacccgg                                                                            >  2:2208816/1‑95 (MQ=255)


                   cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.G.G.GGCGCAGccacccggcc                                                                          <  1:718726/101‑9 (MQ=255)


                   cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.G.G.GGCGCAGccacccggcc                                                                          >  1:3005057/1‑93 (MQ=255)


                   cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.G.G.GGCGCAGccacccggcc                                                                          >  2:4335737/1‑93 (MQ=255)


                    gCAAGGCCCCCTTTTCGCACCCTCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.G.G.GGCGCAGccacccggccg                                                                         <  2:717123/101‑10 (MQ=255)


                    gCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.G.G.GGCGCAGccacccggccg                                                                         >  1:3955382/1‑92 (MQ=255)


                    gCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGACCAAAGGAAG.G.G.GGCGCAGCCGCccggccg                                                                         <  2:4392298/101‑6 (MQ=255)


                                   cGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.G.G.GGCGCAGCC.ACCCGGCCGGgcccc.caaccccgg                                                        >  1:3733976/1‑93 (MQ=255)


                                       cccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGt                                                <  2:2107219/101‑1 (MQ=255)


                                        ccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGtt                                               >  2:533440/1‑101 (MQ=255)


                                           cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                            <  1:663438/101‑1 (MQ=255)


                                           cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                            >  2:2947658/1‑101 (MQ=255)


                                           cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                            <  1:2517397/101‑1 (MQ=255)


                                           cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                            >  1:996430/1‑101 (MQ=255)


                                            aGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGAt                                           >  2:202186/1‑101 (MQ=255)


                                            aGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGAt                                           <  2:3413417/101‑1 (MQ=255)


                                             gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                          >  2:4416418/1‑101 (MQ=255)


                                             gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                          <  2:4437657/101‑1 (MQ=255)


                                              cTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCa                                         >  1:3494710/1‑101 (MQ=255)


                                              cTTACCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGGAGGGCCAAAGGAAG.G.G......AG.CGCACCCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCa                                         >  2:905695/1‑101 (MQ=255)


                                               ttAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAg                                        >  2:3713298/1‑101 (MQ=255)


                                               ttAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAg                                        >  1:564622/1‑101 (MQ=255)


                                                tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                       <  1:1442277/101‑1 (MQ=255)


                                                 aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.G.G.GGCGCAGCC.ACCCGGCCG...CGC.CCCCC.ACCCCGGTCGTTCGATCAGtt                                      >  1:4339176/1‑101 (MQ=255)


                                                 aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                      <  2:3999632/101‑1 (MQ=255)


                                                 aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                      >  1:3207027/1‑101 (MQ=255)


                                                 aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCCTTCGATCAGtt                                      >  2:2135246/1‑101 (MQ=255)


                                                 aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGACGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                      >  2:1817176/1‑101 (MQ=255)


                                                  gCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTc                                     >  2:3800219/1‑101 (MQ=255)


                                                       aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                >  2:2675822/1‑101 (MQ=255)


                                                       aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                >  1:566592/1‑101 (MQ=255)


                                                       aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                >  1:1754427/1‑101 (MQ=255)


                                                        tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                               <  2:1244882/101‑1 (MQ=255)


                                                        tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                               >  1:2194690/1‑101 (MQ=255)


                                                        tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                               >  1:4204945/1‑101 (MQ=255)


                                                        tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                               <  2:2349676/101‑1 (MQ=255)


                                                         gCACGGGCGCGGTCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcga                              >  1:2424205/1‑101 (MQ=255)


                                                         gCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcga                              <  1:2848254/101‑1 (MQ=255)


                                                         gCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGGCGGTCGATCAGTTCTTCCcga                              >  2:2722907/1‑101 (MQ=255)


                                                          cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                             <  2:2192517/101‑1 (MQ=255)


                                                          cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                             <  2:3474612/101‑1 (MQ=255)


                                                           aCGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacg                            >  1:1807860/1‑101 (MQ=255)


                                                             gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                          >  2:2858817/1‑101 (MQ=255)


                                                             gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                          >  1:485732/1‑101 (MQ=255)


                                                              ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                         <  1:2586714/101‑1 (MQ=255)


                                                              ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                         >  1:821679/1‑101 (MQ=255)


                                                               gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCTGGTCGTTCGATCAGTTCTTGCCGACGATGa                        <  2:3976994/101‑1 (MQ=255)


                                                                cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                       >  1:3910591/1‑101 (MQ=255)


                                                                  cgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGt                     <  2:2883855/101‑1 (MQ=255)


                                                                   ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                    >  2:1009380/1‑101 (MQ=255)


                                                                   ggCCAGACGCAGTTTGGCCCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                    <  1:1693145/101‑1 (MQ=255)


                                                                      cAGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                 <  1:1596684/101‑1 (MQ=255)


                                                                       aGACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag                <  1:3069809/101‑1 (MQ=255)


                                                                       aGACGAAGTTTGGCGCAGGGCCAAAGGAA..G.T.GT.GC.GCAGGCAC..CCGGGCGCG.CCCCCAACCCCGGTCGTTCTATCAGTTCTTTGCGACGATGACGTCagag                >  2:738617/1‑101 (MQ=255)


                                                                        gACGCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAgg               >  1:276565/1‑101 (MQ=255)


                                                                           gCAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt            >  1:4432896/1‑101 (MQ=255)


                                                                            cAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCtt           <  1:274372/101‑1 (MQ=255)


                                                                            cAGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCtt           <  2:622617/101‑1 (MQ=255)


                                                                             aGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg          >  1:3418041/1‑101 (MQ=255)


                                                                             aGTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg          <  1:756160/101‑1 (MQ=255)


                                                                              gTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga         >  2:352415/1‑101 (MQ=255)


                                                                              gTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga         <  1:1243131/101‑1 (MQ=255)


                                                                              gTTTGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga         >  1:1066661/1‑101 (MQ=255)


                                                                               tttGGCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat        <  2:154270/101‑1 (MQ=255)


                                                                                 tGGCGCAGGGCCAAATGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGGCGTTCGATCAGTTCTTGCCGACGGTGACATCAGGGACACtgatga      >  2:2994113/1‑101 (MQ=255)


                                                                                 tGGCGCAGGGCCAAAGGAAG.GAG.GC.GCAGCC.ACCC.....GGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga      >  1:2538275/1‑101 (MQ=255)


                                                                                 tGGCGCAGGGCCAAAGGAAG.G.G.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCCGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga      >  2:1792325/1‑101 (MQ=255)


                                                                                 tGGCGCAGGGCCAAAGGAAG.G.G.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga      >  1:270951/1‑101 (MQ=255)


                                                                                  ggCGCAGGGCCAAAGGAAG.G.G.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat     >  2:1589245/1‑101 (MQ=255)


                                                                                  ggCGCAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat     <  2:2798682/101‑1 (MQ=255)


                                                                                     gcAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgc  >  2:4175234/1‑101 (MQ=255)


                                                                                     gcAGGGCCAAAGGAA..G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgc  >  1:3375662/1‑101 (MQ=255)


GGAATTTCAGATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAG.G.G.GG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGC  >  NC_014034/3461272‑3461461

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG