Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,461,379 Δ1 bp 65.5% intergenic (‑89/+41) RCAP_rcc03260 ← / ← RCAP_rcc03261 NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,461,3790A→.65.5% 227.8 ‑0.2 ‑0.1142intergenic (‑89/+41)RCAP_rcc03260/RCAP_rcc03261NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (48/45):  ref base (24/25):  total (72/70)
Fisher's exact test strand distribution p-value = 8.60e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.38e-01

Only 100 of 152 total aligned reads displayed.

ATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGC  >  NC_014034/3461282‑3461461
                                                                                                   |                                                                                   
  cTTTCATCGCAAGGCCCCCTTTTCGCTCCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGcca                                                                                 <  1:233802/101‑2 (MQ=255)
  cTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGcca                                                                                 >  1:2663269/1‑100 (MQ=255)
  cTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGcca                                                                                 <  2:2391141/101‑2 (MQ=255)
   tttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccac                                                                                <  1:4034899/101‑3 (MQ=255)
    ttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacc                                                                               <  2:700507/101‑4 (MQ=255)
    ttCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacc                                                                               >  1:3579669/1‑98 (MQ=255)
    ttCAGCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacc                                                                               <  1:2431777/101‑4 (MQ=255)
     tCATCGCAAGGCCCCCTTTTCGCACCCGCAGTTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccaccc                                                                              >  1:3977250/1‑97 (MQ=255)
     tCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccaccc                                                                              <  1:2965627/101‑5 (MQ=255)
     tCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccaccc                                                                              >  2:1778441/1‑97 (MQ=255)
     tCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccaccc                                                                              >  1:3817244/1‑97 (MQ=255)
     tCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccaccc                                                                              <  1:4000646/101‑5 (MQ=255)
      cATCGCAAGGCCCCCTTTTCGCGCCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccg                                                                             <  2:520795/101‑6 (MQ=255)
      cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccg                                                                             <  2:1377791/101‑6 (MQ=255)
      cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccg                                                                             <  2:2633054/101‑6 (MQ=255)
      cATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccg                                                                             <  1:3106293/101‑6 (MQ=255)
       aTCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccgg                                                                            <  1:4169137/101‑7 (MQ=255)
       aTCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGCTTGGCGCAGGGCCAAAGGAAGGGCGC.GCAGccacccgg                                                                            >  2:2208816/1‑95 (MQ=255)
       aTCGCAAGGCCCCCGTTTCGCACCCGCAGGCTAGCGGGATCCACGGGCGCGGCCAGGCGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGCCGCccgg                                                                            <  2:737691/101‑3 (MQ=255)
         cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccggcc                                                                          >  1:3044255/1‑93 (MQ=255)
         cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccggcc                                                                          <  1:718726/101‑9 (MQ=255)
         cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccggcc                                                                          >  1:3005057/1‑93 (MQ=255)
         cGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccggcc                                                                          <  1:2881134/101‑9 (MQ=255)
          gCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccggccg                                                                         >  2:101023/1‑92 (MQ=255)
          gCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccggccg                                                                         >  2:1243038/1‑92 (MQ=255)
          gCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccggccg                                                                         <  2:499586/101‑10 (MQ=255)
          gCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccggccg                                                                         >  1:3955382/1‑92 (MQ=255)
          gCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGACCAAAGGAAGGG.GGCGCAGCCGCccggccg                                                                         <  2:4392298/101‑6 (MQ=255)
           cAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGccacccggccgc                                                                        >  2:1484451/1‑91 (MQ=255)
                         cGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGCC.ACCCGGCCGGgcccc.caaccccgg                                                        >  1:3733976/1‑93 (MQ=255)
                            aCCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCg                                                 >  2:2040015/1‑101 (MQ=255)
                             cccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGt                                                <  2:2107219/101‑1 (MQ=255)
                              ccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGtt                                               <  2:4343925/101‑1 (MQ=255)
                                gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                             <  2:1952006/101‑1 (MQ=255)
                                 cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                            <  1:2517397/101‑1 (MQ=255)
                                 cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                            >  1:996430/1‑101 (MQ=255)
                                  aGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGAt                                           <  1:3417163/101‑1 (MQ=255)
                                   gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                          <  2:4437657/101‑1 (MQ=255)
                                   gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GC..AGCCAACC.GGCCGCGCCCCCAACCCCGGTCGTTCGATc                                          >  1:3211255/1‑101 (MQ=255)
                                    cTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCa                                         >  1:3494710/1‑101 (MQ=255)
                                    cTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCa                                         <  1:4174863/101‑1 (MQ=255)
                                    cTTAGAAAGATGCGCGGGGCCGCCCCGCCGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCCCAGCC.ACCCCGCCG...CG..CCCCCAACCCCGGTCGTTCGATCa                                         <  2:567523/101‑1 (MQ=255)
                                    cTTACCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGGAGGGCCAAAGGAAGGG......AG.CGCACCCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCa                                         >  2:905695/1‑101 (MQ=255)
                                     ttAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAg                                        >  1:564622/1‑101 (MQ=255)
                                     ttAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAg                                        >  2:3713298/1‑101 (MQ=255)
                                      tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                       <  1:1442277/101‑1 (MQ=255)
                                      tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                       >  1:3730562/1‑101 (MQ=255)
                                       aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGCC.ACCCGGCCG...CGC.CCCCC.ACCCCGGTCGTTCGATCAGtt                                      >  1:4339176/1‑101 (MQ=255)
                                       aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                      <  2:3999632/101‑1 (MQ=255)
                                       aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCCTTCGATCAGtt                                      >  2:2135246/1‑101 (MQ=255)
                                       aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGACGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                      >  2:1817176/1‑101 (MQ=255)
                                        gCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTc                                     >  2:3800219/1‑101 (MQ=255)
                                         cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                    >  1:708295/1‑101 (MQ=255)
                                            gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                 >  1:2642963/1‑101 (MQ=255)
                                            gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                 >  2:2197475/1‑101 (MQ=255)
                                             aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                >  1:1754427/1‑101 (MQ=255)
                                             aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                >  2:2675822/1‑101 (MQ=255)
                                             aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                >  1:566592/1‑101 (MQ=255)
                                              tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                               >  2:3434680/1‑101 (MQ=255)
                                              tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                               >  1:2194690/1‑101 (MQ=255)
                                              tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                               <  2:51690/101‑1 (MQ=255)
                                              tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                               <  2:2349676/101‑1 (MQ=255)
                                               gCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGGCGGTCGATCAGTTCTTCCcga                              >  2:2722907/1‑101 (MQ=255)
                                                cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                             <  1:4440837/101‑1 (MQ=255)
                                                cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                             <  2:3474612/101‑1 (MQ=255)
                                                 aCGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacg                            >  1:1807860/1‑101 (MQ=255)
                                                   gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                          >  2:2858817/1‑101 (MQ=255)
                                                   gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                          >  1:485732/1‑101 (MQ=255)
                                                   gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                          >  1:3089612/1‑101 (MQ=255)
                                                    ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                         <  1:2586714/101‑1 (MQ=255)
                                                    ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                         >  1:821679/1‑101 (MQ=255)
                                                     gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCTGGTCGTTCGATCAGTTCTTGCCGACGATGa                        <  2:3976994/101‑1 (MQ=255)
                                                      cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                       >  1:3910591/1‑101 (MQ=255)
                                                      cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                       <  2:3867993/101‑1 (MQ=255)
                                                       gcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACg                      <  2:698467/101‑1 (MQ=255)
                                                        cgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGt                     >  2:4315298/1‑101 (MQ=255)
                                                         ggCCAGACGCAGTTTGGCCCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                    <  1:1693145/101‑1 (MQ=255)
                                                          gCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCa                   >  1:4394634/1‑101 (MQ=255)
                                                          gCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.AG.GG.GC.GC..AACCAACC.GGCGGCGGCCCCAACGCCGGTCGTTCGCTCAGTTCTTGCCGCCGCTGACGTCa                   >  2:2178736/1‑101 (MQ=255)
                                                           ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTccg                  >  2:3184684/1‑99 (MQ=255)
                                                            cAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                 <  1:1596684/101‑1 (MQ=255)
                                                             aGACGAAGTTTGGCGCAGGGCCAAAGGAA.GT.GT.GC.GCAGGCAC..CCGGGCGCG.CCCCCAACCCCGGTCGTTCTATCAGTTCTTTGCGACGATGACGTCagag                >  2:738617/1‑101 (MQ=255)
                                                              gACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAgg               >  1:276565/1‑101 (MQ=255)
                                                                  cAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCtt           >  2:1557234/1‑101 (MQ=255)
                                                                  cAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCtt           <  2:622617/101‑1 (MQ=255)
                                                                  cAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCtt           <  1:274372/101‑1 (MQ=255)
                                                                   aGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg          <  1:756160/101‑1 (MQ=255)
                                                                    gTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga         >  1:1066661/1‑101 (MQ=255)
                                                                    gTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga         >  1:1232571/1‑101 (MQ=255)
                                                                    gTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga         >  2:352415/1‑101 (MQ=255)
                                                                     tttGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat        <  2:154270/101‑1 (MQ=255)
                                                                       tGGCGCAGGGCCAAATGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGGCGTTCGATCAGTTCTTGCCGACGGTGACATCAGGGACACtgatga      >  2:2994113/1‑101 (MQ=255)
                                                                       tGGCGCAGGGCCAAAGGAAGGG.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga      >  1:270951/1‑101 (MQ=255)
                                                                       tGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga      <  2:2943594/101‑1 (MQ=255)
                                                                        ggCGCAGGGCCAAAGGAAGGG.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat     >  2:1589245/1‑101 (MQ=255)
                                                                        ggCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat     <  2:2798682/101‑1 (MQ=255)
                                                                          cgcAGGGCCAAAGGAAGGG.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg   >  1:1282155/1‑101 (MQ=255)
                                                                          cgcAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg   >  2:2880198/1‑101 (MQ=255)
                                                                           gcAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgc  >  2:4175234/1‑101 (MQ=255)
                                                                           gcAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgc  >  1:3375662/1‑101 (MQ=255)
                                                                                                   |                                                                                   
ATCTTTCATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGC  >  NC_014034/3461282‑3461461

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG