Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,461,384 Δ3 bp 100% intergenic (‑94/+34) RCAP_rcc03260 ← / ← RCAP_rcc03261 NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,461,3840A→.100.0% 35.5 103intergenic (‑94/+36)RCAP_rcc03260/RCAP_rcc03261NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (46/45):  ref base (9/0):  total (56/47)
Fisher's exact test strand distribution p-value = 3.81e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.73e-01
*NC_0140343,461,3850C→.100.0% 25.1 101intergenic (‑95/+35)RCAP_rcc03260/RCAP_rcc03261NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (46/45):  ref base (9/0):  total (56/45)
Fisher's exact test strand distribution p-value = 3.81e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.73e-01
*NC_0140343,461,3860C→.100.0% 25.9 101intergenic (‑96/+34)RCAP_rcc03260/RCAP_rcc03261NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (46/45):  ref base (9/0):  total (56/45)
Fisher's exact test strand distribution p-value = 3.81e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.10e-01

Only 100 of 111 total aligned reads displayed.

ATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGG.G.GG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGC  >  NC_014034/3461289‑3461461
                                                                                                  |||                                                                            
aTCGCAAGGCCCCCGTTTCGCACCCGCAGGCTAGCGGGATCCACGGGCGCGGCCAGGCGCAGTTTGGCGCAGGGCCAAAGGAAGG.G.GGCGCAGCCGCccgg                                                                            <  2:737691/101‑3 (MQ=255)
   gCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGACCAAAGGAAGG.G.GGCGCAGCCGCccggccg                                                                         <  2:4392298/101‑6 (MQ=255)
                  cGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGG.G.GGCGCAGCC.ACCCGGCCGGgcccc.caaccccgg                                                        >  1:3733976/1‑93 (MQ=255)
                     aCCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCg                                                 >  2:2040015/1‑101 (MQ=255)
                      cccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGt                                                <  2:2107219/101‑1 (MQ=255)
                       ccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGtt                                               <  2:2858808/101‑1 (MQ=255)
                       ccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGtt                                               >  2:533440/1‑101 (MQ=255)
                       ccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGtt                                               <  2:2679688/101‑1 (MQ=255)
                       ccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGtt                                               <  2:4343925/101‑1 (MQ=255)
                        cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                              <  1:1719150/101‑1 (MQ=255)
                         gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                             <  2:1952006/101‑1 (MQ=255)
                          cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                            <  1:663438/101‑1 (MQ=255)
                          cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                            >  1:996430/1‑101 (MQ=255)
                          cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                            <  1:2517397/101‑1 (MQ=255)
                          cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                            >  2:2947658/1‑101 (MQ=255)
                           aGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGAt                                           >  2:202186/1‑101 (MQ=255)
                           aGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGAt                                           <  1:3417163/101‑1 (MQ=255)
                           aGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGAt                                           <  2:3413417/101‑1 (MQ=255)
                            gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                          <  2:4437657/101‑1 (MQ=255)
                            gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GC..AGCCAACC.GGCCGCGCCCCCAACCCCGGTCGTTCGATc                                          >  1:3211255/1‑101 (MQ=255)
                             cTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCa                                         <  1:4174863/101‑1 (MQ=255)
                             cTTAGAAAGATGCGCGGGGCCGCCCCGCCGCAGTTTGGCGCAGGGCCAAAGGAAGG.G.GGCCCAGCC.ACCCCGCCG...CG..CCCCCAACCCCGGTCGTTCGATCa                                         <  2:567523/101‑1 (MQ=255)
                              ttAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAg                                        >  2:3713298/1‑101 (MQ=255)
                              ttAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAg                                        >  1:564622/1‑101 (MQ=255)
                               tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                       <  1:520629/101‑1 (MQ=255)
                               tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                       <  1:1442277/101‑1 (MQ=255)
                               tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                       >  1:3730562/1‑101 (MQ=255)
                                aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGG.G.GGCGCAGCC.ACCCGGCCG...CGC.CCCCC.ACCCCGGTCGTTCGATCAGtt                                      >  1:4339176/1‑101 (MQ=255)
                                aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                      <  2:3999632/101‑1 (MQ=255)
                                aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGtt                                      >  1:3207027/1‑101 (MQ=255)
                                aGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCCTTCGATCAGtt                                      >  2:2135246/1‑101 (MQ=255)
                                  cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                    >  1:708295/1‑101 (MQ=255)
                                   aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                   <  2:3955126/101‑1 (MQ=255)
                                     gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                 >  2:2197475/1‑101 (MQ=255)
                                     gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                 >  1:2642963/1‑101 (MQ=255)
                                      aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                >  2:2675822/1‑101 (MQ=255)
                                      aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                >  1:1754427/1‑101 (MQ=255)
                                      aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                >  1:566592/1‑101 (MQ=255)
                                       tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                               >  2:3434680/1‑101 (MQ=255)
                                       tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                               <  2:1244882/101‑1 (MQ=255)
                                       tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                               >  1:4204945/1‑101 (MQ=255)
                                       tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                               <  2:2349676/101‑1 (MQ=255)
                                       tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                               >  1:2194690/1‑101 (MQ=255)
                                       tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                               <  2:51690/101‑1 (MQ=255)
                                        gCACGGGCGCGGTCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcga                              >  1:2424205/1‑101 (MQ=255)
                                        gCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcga                              <  1:2848254/101‑1 (MQ=255)
                                        gCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGGCGGTCGATCAGTTCTTCCcga                              >  2:2722907/1‑101 (MQ=255)
                                         cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                             <  1:4440837/101‑1 (MQ=255)
                                         cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                             <  2:2192517/101‑1 (MQ=255)
                                         cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                             <  1:3282765/101‑1 (MQ=255)
                                         cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                             <  2:3474612/101‑1 (MQ=255)
                                          aCGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacg                            >  2:3756480/1‑101 (MQ=255)
                                           cGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGCTCAGTTCTTGCcgacga                           >  1:743932/1‑101 (MQ=255)
                                            gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                          >  1:485732/1‑101 (MQ=255)
                                            gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                          >  2:2858817/1‑101 (MQ=255)
                                            gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                          >  1:3089612/1‑101 (MQ=255)
                                             ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                         >  1:821679/1‑101 (MQ=255)
                                             ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                         <  1:2586714/101‑1 (MQ=255)
                                              gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCTGGTCGTTCGATCAGTTCTTGCCGACGATGa                        <  2:3976994/101‑1 (MQ=255)
                                               cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                       <  2:3867993/101‑1 (MQ=255)
                                               cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                       >  1:3910591/1‑101 (MQ=255)
                                                gcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACg                      <  2:698467/101‑1 (MQ=255)
                                                gcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACg                      <  1:4051574/101‑1 (MQ=255)
                                                 cgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGt                     >  2:4315298/1‑101 (MQ=255)
                                                 cgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGt                     <  2:2883855/101‑1 (MQ=255)
                                                  ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                    >  2:1009380/1‑101 (MQ=255)
                                                  ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                    >  2:2125424/1‑101 (MQ=255)
                                                  ggCCAGACGCAGTTTGGCCCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                    <  1:1693145/101‑1 (MQ=255)
                                                   gCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.A.G.GG.GC.GC..AACCAACC.GGCGGCGGCCCCAACGCCGGTCGTTCGCTCAGTTCTTGCCGCCGCTGACGTCa                   >  2:2178736/1‑101 (MQ=255)
                                                    ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTccg                  >  2:3184684/1‑99 (MQ=255)
                                                     cAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                 <  1:1596684/101‑1 (MQ=255)
                                                     cAGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                 <  1:3936270/101‑1 (MQ=255)
                                                      aGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag                <  1:3069809/101‑1 (MQ=255)
                                                      aGACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag                <  1:1563014/101‑1 (MQ=255)
                                                      aGACGAAGTTTGGCGCAGGGCCAAAGGAA.G.T.GT.GC.GCAGGCAC..CCGGGCGCG.CCCCCAACCCCGGTCGTTCTATCAGTTCTTTGCGACGATGACGTCagag                >  2:738617/1‑101 (MQ=255)
                                                       gACGCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAgg               >  2:4120964/1‑101 (MQ=255)
                                                          gCAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt            >  1:4432896/1‑101 (MQ=255)
                                                           cAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCtt           <  1:274372/101‑1 (MQ=255)
                                                           cAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCtt           <  2:622617/101‑1 (MQ=255)
                                                           cAGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCtt           >  2:1557234/1‑101 (MQ=255)
                                                            aGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg          >  1:3418041/1‑101 (MQ=255)
                                                            aGTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg          <  1:913877/101‑1 (MQ=255)
                                                             gTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga         >  2:352415/1‑101 (MQ=255)
                                                             gTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga         >  1:1066661/1‑101 (MQ=255)
                                                             gTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga         >  1:1232571/1‑101 (MQ=255)
                                                             gTTTGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga         <  1:1243131/101‑1 (MQ=255)
                                                              tttGGCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat        <  2:154270/101‑1 (MQ=255)
                                                                tGGCGCAGGGCCAAATGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGGCGTTCGATCAGTTCTTGCCGACGGTGACATCAGGGACACtgatga      >  2:2994113/1‑101 (MQ=255)
                                                                tGGCGCAGGGCCAAAGGAAGGAG.GC.GCAGCC.ACCC.....GGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga      >  1:2538275/1‑101 (MQ=255)
                                                                tGGCGCAGGGCCAAAGGAAGG.GAGC.GCAGCC.ACCCTGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga      <  2:743852/101‑1 (MQ=255)
                                                                tGGCGCAGGGCCAAAGGAAGG.G.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCCGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga      >  2:1792325/1‑101 (MQ=255)
                                                                tGGCGCAGGGCCAAAGGAAGG.G.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga      >  1:270951/1‑101 (MQ=255)
                                                                 ggCGCAGGGCCAAAGGAAGG.G.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat     >  2:1589245/1‑101 (MQ=255)
                                                                 ggCGCAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat     <  2:2798682/101‑1 (MQ=255)
                                                                   cgcAGGGCCAAAGGAAGG.G.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg   >  1:2120997/1‑101 (MQ=255)
                                                                   cgcAGGGCCAAAGGAAGG.G.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg   >  1:1282155/1‑101 (MQ=255)
                                                                   cgcAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg   >  2:2880198/1‑101 (MQ=255)
                                                                    gcAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgc  <  1:363640/101‑1 (MQ=255)
                                                                    gcAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgc  >  1:3375662/1‑101 (MQ=255)
                                                                    gcAGGGCCAAAGGAA.G.G.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcgc  >  2:4175234/1‑101 (MQ=255)
                                                                                                  |||                                                                            
ATCGCAAGGCCCCCTTTTCGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGG.G.GG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGC  >  NC_014034/3461289‑3461461

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG