Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,596,943 Δ1 bp 4.7% coding (161/1467 nt) RCAP_rcc03392 → hemolysin D

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,596,9430C→. 4.7% 14.7 ‑0.3 ‑1.2148coding (161/1467 nt)RCAP_rcc03392hemolysin D
Reads supporting (aligned to +/- strand):  new base (5/2):  ref base (86/54):  total (92/56)
Fisher's exact test strand distribution p-value = 7.09e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.65e-02

Only 100 of 190 total aligned reads displayed.

CAATCCCCAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTGGTCGGTG  >  NC_014034/3596844‑3597043
                                                                                                     |                                                                                                     
cAATCCCCAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.cc                                                                                                      >  2:1637489/1‑101 (MQ=255)
 aaTCCCCAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCACGCCCGCC.GGAGCGAACAGGAAG.ccc                                                                                                     >  2:731456/1‑101 (MQ=255)
     cccAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.ccccccc                                                                                                 <  1:3898822/101‑1 (MQ=255)
     cccAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.ccccccc                                                                                                 >  1:3280182/1‑101 (MQ=255)
     cccAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.ccccccc                                                                                                 >  1:270803/1‑101 (MQ=255)
       cAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.ccccccccc                                                                                               >  2:2674993/1‑101 (MQ=255)
        aGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.cccccccccc                                                                                              >  1:3047631/1‑101 (MQ=255)
        aGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.cccccccccc                                                                                              >  1:2656036/1‑101 (MQ=255)
        aGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.cccccccccc                                                                                              >  1:1511559/1‑101 (MQ=255)
          ccccAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCgg                                                                                            <  1:2301302/98‑1 (MQ=255)
          ccGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCgc                                                                                            >  2:970449/1‑100 (MQ=255)
           cgccgcccccccgccccccTCGGCCCGCCCGCGTTCCAGGCGGCAGAGACCGGCCTCCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGc                                                                                           <  1:3722496/95‑1 (MQ=255)
           cGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGc                                                                                           <  2:3255746/101‑1 (MQ=255)
             cAAGCCCCCCGCCCCCCTCGGCCCGCCCGCGTTCCAGGCGGCAGAGCCCGCCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCg                                                                                         <  2:3032892/101‑1 (MQ=255)
             cAAGCCCCCCGCCCCCCCCGGCCCGGCCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCg                                                                                         <  2:4033059/101‑1 (MQ=255)
               aGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAg                                                                                       >  2:1693047/1‑101 (MQ=255)
                gccccccgccccccTCGGCCCGACCGCGTTGCAGGCGGCAGGGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAgg                                                                                      <  2:2853050/101‑1 (MQ=255)
                   ccccgccccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGgcgc                                                                                   <  1:1009672/101‑1 (MQ=255)
                    cccgccccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAGCCCCCCCCCCCGGCCGCGgcgc                                                                                   >  1:2877411/1‑101 (MQ=255)
                    cccgccccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAgggccg                                                                                  >  2:3837530/1‑97 (MQ=255)
                    cccgccccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGcgcg                                                                                  >  2:2710344/1‑101 (MQ=255)
                      cgccccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGa                                                                                >  2:2715235/1‑101 (MQ=255)
                       gccccccTCGGCCCGAGCGGGTTGCAGGCGGCACAGACCGGCCTTCCCGCCCCGCCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGAt                                                                               <  2:1242079/101‑1 (MQ=255)
                       gccccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGGACCGGAAG.CCCCCCCCCCGGCCgggcgggggac                                                                               >  1:3863903/1‑91 (MQ=255)
                         cccccTCGGCCCGCCCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGAtct                                                                             <  1:4201160/101‑1 (MQ=255)
                         cccacTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGAtct                                                                             >  2:1359805/1‑101 (MQ=255)
                          ccccTGGGCCCGACCGCGTTGCGGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGAtctc                                                                            <  2:848380/101‑1 (MQ=255)
                          ccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGGGGGCCGGAactc                                                                            >  2:4371247/1‑101 (MQ=255)
                          ccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGAtctc                                                                            <  2:30926/101‑1 (MQ=255)
                          ccccTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGAcctc                                                                            >  1:864110/1‑101 (MQ=255)
                              tCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCCccc                                                                        >  1:4238612/1‑101 (MQ=255)
                               cGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCTCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGcccc                                                                       <  2:990105/101‑1 (MQ=255)
                                ggCCCGCCCCCTTTGGAGGCGGACGAGAGCCGCCTTCCCGGCCCGGCCCGCC.GGAGCGAACCGGAAA.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCg                                                                      <  2:3901406/101‑1 (MQ=255)
                                ggCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGGTCCCGCCCCg                                                                      >  1:1664000/1‑101 (MQ=255)
                                ggCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGCTCTCGCCCCg                                                                      >  1:1163373/1‑101 (MQ=255)
                                ggCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCg                                                                      >  2:4095651/1‑101 (MQ=255)
                                ggCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCCCCCCCCg                                                                      >  1:2065123/1‑101 (MQ=255)
                                 gCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCCGCCGCGGCGCGGGGCTCGcccccg                                                                     >  1:3748133/1‑99 (MQ=255)
                                   ccGCCCGCGTTGCGGGCGGCAGAGCCCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGt                                                                   <  2:2093669/101‑1 (MQ=255)
                                   ccGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCgggg                                                                   >  1:1409784/1‑100 (MQ=255)
                                    cGACCGCGTTGCAGGCGGCAGAGACCGTCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.GCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTg                                                                  >  2:2977696/1‑101 (MQ=255)
                                     caCCGCGTTGCGGGCGGCAGGGGCCGGCCTTCCCGCCCCGGCCCCCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTgg                                                                 <  2:3754156/100‑1 (MQ=255)
                                      cccccgTTGCCGGGCGCAAGGACCGGCCCCCCCCCCCCCGCCCGCC.GGAGCAAACCGGAAC.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGgc                                                                <  1:3984668/97‑1 (MQ=255)
                                      cccGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGgc                                                                <  1:600183/100‑1 (MQ=255)
                                      aCCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGgc                                                                >  1:1490688/1‑101 (MQ=255)
                                         gcgTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGgcgcg                                                             >  2:3777737/1‑100 (MQ=255)
                                         gccgtGCGGGCGGCAGAGCCCGCCCTCCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCgct                                                             <  2:3747880/97‑1 (MQ=255)
                                          ggtggCAGGGGGCGGAGCCCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCgctg                                                            <  2:2152878/97‑1 (MQ=255)
                                          cgTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGgggcgg                                                            >  1:2275024/1‑99 (MQ=255)
                                           gTTGCAGGCGGCAGACGCCGGCCCGCCCGCCCCGGCCCGCC.GGGGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCgctgc                                                           <  1:1583176/101‑1 (MQ=255)
                                             ggagggcggcAGAG.CCGGCCCTCCCCCCCCGGCCCCCCGGGAGCAACCCGGAA..CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGa                                                        <  2:3504704/97‑1 (MQ=255)
                                               cAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGCGGCGCGGATCTCGCCCCGGGGGGCGCCGCTGac                                                       >  1:1827298/1‑100 (MQ=255)
                                               cAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGAt                                                       <  2:1884653/101‑1 (MQ=255)
                                               cAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGACCTCGCCCCGGGTGGCGCTGCTGAt                                                       >  1:3541926/1‑101 (MQ=255)
                                                aGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGGGGGTCTCGCCCCGGGGGGGGCTGCTGGTc                                                      >  2:2535075/1‑101 (MQ=255)
                                                aGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATc                                                      <  1:725560/101‑1 (MQ=255)
                                                 ggcggcAGCGGCCGCCCCCCCCCCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCg                                                     <  1:3023499/101‑1 (MQ=255)
                                                  gcggcAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCgg                                                    <  2:907775/101‑1 (MQ=255)
                                                    ggcAGGGCCCGGCCTCCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.t                                                 <  2:4216735/101‑1 (MQ=255)
                                                    ggcAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGCGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGCTCCGC.t                                                 >  1:1298041/1‑101 (MQ=255)
                                                       gagggccccccTTCCCGCCCCGGCCCGCC.GGGGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATg                                              <  2:552539/92‑1 (MQ=255)
                                                         aacccGGCCTTCCCGCCCCGGCCCGCG.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTc                                            <  2:2005703/98‑1 (MQ=255)
                                                          gaCCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCCGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCCGCTGATCGGC.TATGTCa                                           >  1:3837313/1‑101 (MQ=255)
                                                            ccGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGC.TATGTCACg                                         >  1:3684855/1‑101 (MQ=255)
                                                            ccGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGCGGCGCTGGTGATCGGC.TATGTCACg                                         >  2:1180271/1‑101 (MQ=255)
                                                             cGGCCTTCCCGCCCCGGCCCGCC.GGGGCGAACCTGAAG..CCCCCCCCCGGCCGAAGCGCCGATCTCGACCCCTGCGGTGCTGCTGATCGGC.TATGCCACGCt                                       >  2:2622430/1‑101 (MQ=255)
                                                               gcccccccCCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCtt                                      <  1:3190647/96‑1 (MQ=255)
                                                               cccTCCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAC.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCtt                                      <  2:1827174/100‑1 (MQ=255)
                                                                ccTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGC.TTTGTCACGCTTg                                     >  2:2378757/1‑101 (MQ=255)
                                                                   tCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCgg                                  <  1:4095911/101‑1 (MQ=255)
                                                                    cccGCCCCGGCCCGCC.GGAGCGAACCGGAAG..CCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGtt                                >  1:3365214/1‑101 (MQ=255)
                                                                    cccCCCCCGGCCCGCC.GGAGCGAA.CGGAAA.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGtt                                <  2:3863650/101‑1 (MQ=255)
                                                                      cGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGGGGCGCGGATCCCGCCCCGGGGGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTc                               >  1:1070428/1‑101 (MQ=255)
                                                                       gCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCCGCCGCGGCGCGGATCTCGCCCCGGGGGGCGGCGCTGCTTGGC.TATGTCACCCTTCCGGTTCt                              >  2:2318310/1‑101 (MQ=255)
                                                                            ggCCCCGC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCg                         <  1:3658223/101‑1 (MQ=255)
                                                                             gCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCgg                        <  1:897176/101‑1 (MQ=255)
                                                                                cgcc.gGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGGGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCggggg                     >  1:679858/1‑101 (MQ=255)
                                                                                cgcc.gGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCggggg                     >  1:1883311/1‑101 (MQ=255)
                                                                                 gcc.gGAGCGAACCGGAAG.CCCCCCCCCCGCCCGGGGCGGGGATCCCGCCCCGGG.GGCGCCGCTGATCGGCTTATGTCACCCTTCCGGTTCGGGCGgggggt                    >  2:1714741/1‑100 (MQ=255)
                                                                                 gcc.gGAGCGAACCGGAAG..CCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCt                   >  2:4056903/1‑101 (MQ=255)
                                                                                 ccc.gGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGc                    <  2:3755628/100‑1 (MQ=255)
                                                                                  cc.gGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCCGCTGATCGGC.TAGGTCACGCTTGCGGTTCTGGTGGGGGGCt                   >  1:584188/1‑101 (MQ=255)
                                                                                   cccggaacaaCCCGAAC.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCt                   <  1:2602691/93‑1 (MQ=255)
                                                                                   c.gGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCtt                  >  2:1979433/1‑101 (MQ=255)
                                                                                   c.gGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCtt                  <  2:1163282/101‑1 (MQ=255)
                                                                                   c.gGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCtt                  <  2:585143/101‑1 (MQ=255)
                                                                                     cggacGAACGGGGGG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTc                 <  2:1038132/97‑1 (MQ=255)
                                                                                      gAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGCTCTCGCCCCGGGTGGCGCTGCGGATCGGC.TATGTCACGCTTGCGGTTCTCGTCGGGGGCTTCg                >  1:2987274/1‑101 (MQ=255)
                                                                                      gAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCg                >  1:4400753/1‑101 (MQ=255)
                                                                                       aGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGGCGGGGGGTTCgg               >  2:15022/1‑101 (MQ=255)
                                                                                        gCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCggg              <  2:2274879/101‑1 (MQ=255)
                                                                                         cGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGAATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGc             <  1:3801290/101‑1 (MQ=255)
                                                                                           aaCCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCtt           >  1:1026811/1‑101 (MQ=255)
                                                                                            aCCGGAAG.CCCCCCCCCCGGCCGGGGCGCGGACCTCGCCCCGGGGGGCGCTGCGGATCGGC.TATGTCACGCTTGCGGTTCGGGGGGGGGGCTTCGGGCttt          >  2:3280028/1‑101 (MQ=255)
                                                                                            aCCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCttt          <  1:526694/101‑1 (MQ=255)
                                                                                            aCCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCttt          >  1:2097459/1‑101 (MQ=255)
                                                                                               ggAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTGGt       <  2:4191129/101‑1 (MQ=255)
                                                                                               ggAAG..CCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTGGTc      <  2:3650197/101‑1 (MQ=255)
                                                                                                gAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGGGGCGCTGCTGATCGGC.TATGTCCCGCTTGCGGGTCTGGGCGGGGGCTTCGGGGTTTGGtg      >  1:1898302/1‑100 (MQ=255)
                                                                                                   g.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTGGTCggt   <  1:970534/101‑1 (MQ=255)
                                                                                                     |                                                                                                     
CAATCCCCAGCCGCAAGCCCCCCGCCCCCCTCGGCCCGACCGCGTTGCAGGCGGCAGAGACCGGCCTTCCCGCCCCGGCCCGCC.GGAGCGAACCGGAAG.CCCCCCCCCCGGCCGAGGCGCGGATCTCGCCCCGGGTGGCGCTGCTGATCGGC.TATGTCACGCTTGCGGTTCTGGTCGGGGGCTTCGGGCTTTGGTCGGTG  >  NC_014034/3596844‑3597043

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG