Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 408,487 Δ1 bp 100% intergenic (+105/+43) glnA1 → / ← dapA glutamine synthetase/dihydrodipicolinate synthase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034408,4870A→.100.0% 363.1 110intergenic (+105/+43)glnA1/dapAglutamine synthetase/dihydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  new base (73/35):  ref base (0/0):  total (75/35)

Only 100 of 130 total aligned reads displayed.

TGGGGGCTCTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGCAAAGACGGGGG.....GC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCG  >  NC_014034/408388‑408569
                                                                                                      |                                                                                             
tGGGGGCTCTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGCaa                                                                                              >  2:1888375/1‑101 (MQ=255)
tGGGGGCTCTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGCaa                                                                                              >  2:4414898/1‑101 (MQ=255)
tGGGGGCTCTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCCTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGCaa                                                                                              >  1:4444243/1‑101 (MQ=255)
gggggcCCCTCCCCCCAGACCCCGGGTATTTTGGGGGCAAGAGAAAG.CCGATTTCCTTCATTTTGCCGAAAATTT.CCCGGGGGGAGGCTT.GCTGAAAGCaa                                                                                              <  1:1026399/100‑1 (MQ=255)
 gggggCTCTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGCaag                                                                                             >  1:2490310/1‑100 (MQ=255)
 gggagacgggCCCCCCGTGCCCCGGGATATTCGCGGAAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGCaag                                                                                             <  2:2051885/92‑2 (MQ=21)
  ggggCTCTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGCaaga                                                                                            >  2:2301447/1‑99 (MQ=255)
  ggggCTCTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAACaagc                                                                                            >  2:3956188/1‑99 (MQ=255)
   gggCTCTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGCaagac                                                                                           <  2:3045127/101‑4 (MQ=255)
   gggCTCTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAACCaagac                                                                                           >  1:1322724/1‑98 (MQ=255)
    ggCTCTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGGAAGAaaaaag                                                                                          >  1:2353833/1‑98 (MQ=255)
      ctctGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACggg                                                                                       >  1:626754/1‑101 (MQ=255)
      ctctGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACggg                                                                                       >  2:2963218/1‑101 (MQ=255)
       tctGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACgggg                                                                                      >  1:2402885/1‑101 (MQ=255)
       tctGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACgggg                                                                                      >  2:2515327/1‑101 (MQ=255)
        ctGCCCCCAGCCCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACggggg                                                                                     <  2:1872354/101‑1 (MQ=255)
        ctGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACggggg                                                                                     >  1:386994/1‑101 (MQ=255)
         tGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGGCCTTCATCTTGCCGCAAATAT.CCCGGGGGGGGGCTT.GCTGAAAGC.AAGACggggg.....g                                                                               >  2:1257335/1‑101 (MQ=255)
          gCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gc                                                                              >  2:2845506/1‑101 (MQ=255)
          ccccccAGCCCCCGGGGATTTTGGCGGAAAGATGAAC.CCGATGTCCTTCATCTTGCCGAAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gc                                                                              <  2:2228571/100‑1 (MQ=25)
           tcgcctcaCCCCCGGGATATTTGGGGCAAGATAAAC.CCCAT.CCTTTCACCTTGCCGCAAATAT.CCCGGGGGGTGAATTGGCCGAAGGCCAAGAGgggggcg                                                                                   >  2:2149309/8‑99 (MQ=25)
           cccccAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gca                                                                             >  1:1204423/1‑100 (MQ=255)
           cccccAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gca                                                                             <  1:1348590/101‑2 (MQ=255)
            cgcctcaCCCCGGGGATATTTGGGGCAAGATAAAC.CCCAT.CCTTTCACCTTGCCGCAAATAT.CCCGGGGGGTGAATTGGCCGAAGGCCAAGAGgggggcag                                                                                  >  2:425627/7‑98 (MQ=21)
            ccccCGGCCCCGGGGAATTTTGGGGCAAAGAGAAGCCCGTTGTCTTTCTTC.TGCCCAAAATTTCCCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gca                                                                             <  2:3126802/101‑2 (MQ=255)
            ccccAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gcag                                                                            <  2:2684992/101‑3 (MQ=255)
            ccccAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gcag                                                                            >  1:4414609/1‑99 (MQ=255)
             cccAGACCCCGGGGATATTTGGGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gcaga                                                                           <  1:4435016/101‑4 (MQ=25)
              ccAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gcagag                                                                          <  2:4234465/101‑5 (MQ=255)
              ccAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gcagag                                                                          >  2:1700875/1‑97 (MQ=255)
               cAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gcagagc                                                                         <  2:2490147/101‑6 (MQ=255)
               cAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gcagagc                                                                         <  2:4019496/101‑6 (MQ=255)
                agccccccGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gcagagcc                                                                        <  2:4067876/98‑7 (MQ=25)
                 gACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gcagagccc                                                                       <  2:2663694/101‑8 (MQ=25)
                  gcccccGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gcagagcccc                                                                      <  2:1468045/100‑9 (MQ=25)
                  aCCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....Gcagcgtccc                                                                      >  2:1424686/1‑93 (MQ=25)
                                tGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGCAAGNCNNNGGGCAGA.GC.....CCCC.CGCACAGGGCGcc                                                       >  1:2386122/1‑101 (MQ=21)
                                tGCGGCAAGAGGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGcc                                                       <  2:491217/101‑1 (MQ=18)
                                 gCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCa                                                      >  2:537136/1‑101 (MQ=21)
                                    gCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCaaaa                                                   >  2:3443726/1‑101 (MQ=21)
                                      aaGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAgg                                                 >  1:1565829/1‑101 (MQ=21)
                                       aGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGga                                                <  2:1837096/101‑1 (MQ=21)
                                         aTGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGgacc                                              >  1:1156226/1‑101 (MQ=21)
                                         aTGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGgacc                                              <  2:2348725/101‑1 (MQ=21)
                                          tGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGgaccg                                             <  2:1388660/101‑1 (MQ=21)
                                          tGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGgaccg                                             <  1:2594633/101‑1 (MQ=21)
                                           gAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGgaccga                                            >  1:96501/1‑101 (MQ=21)
                                           gAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGgaccga                                            >  1:1645602/1‑101 (MQ=21)
                                           gAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGgaccga                                            >  2:2331352/1‑101 (MQ=21)
                                            aaG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGgaccgac                                           >  1:563762/1‑101 (MQ=21)
                                              g.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACAGACCt                                         >  2:1339261/1‑101 (MQ=18)
                                                ccGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTc                                        <  2:3291327/101‑1 (MQ=21)
                                                 cGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCa                                       <  2:2041322/101‑1 (MQ=21)
                                                  gATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAg                                      >  1:3199251/1‑101 (MQ=21)
                                                  gATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....GCAGAGTCCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAg                                      >  1:1869477/1‑101 (MQ=21)
                                                    tGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGAt                                    >  1:3319484/1‑101 (MQ=21)
                                                     gTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATc                                   <  2:3783/101‑1 (MQ=21)
                                                     gTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATc                                   >  1:3186489/1‑101 (MQ=21)
                                                      tCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCa                                  <  1:1831113/101‑1 (MQ=21)
                                                      tCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCa                                  >  1:1849518/1‑101 (MQ=21)
                                                      tCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCa                                  >  2:129135/1‑101 (MQ=21)
                                                      tCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCa                                  >  2:2849959/1‑101 (MQ=21)
                                                       ccTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAg                                 >  1:2333565/1‑101 (MQ=21)
                                                       ccTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAg                                 >  2:459350/1‑101 (MQ=21)
                                                       ccTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAg                                 >  2:1853000/1‑101 (MQ=21)
                                                       ccTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTAGCCAAAAGGACCGACCTCAGATCAg                                 >  2:301315/1‑101 (MQ=18)
                                                        cTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGc                                >  1:1371001/1‑101 (MQ=21)
                                                        cTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGc                                <  2:4414337/101‑1 (MQ=21)
                                                        cTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGc                                >  2:4356696/1‑101 (MQ=21)
                                                        cTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGc                                >  2:2111752/1‑101 (MQ=21)
                                                        cTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGG.....GCAGAGACCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGc                                >  2:1988322/1‑101 (MQ=21)
                                                         ttCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGcc                               <  2:1838755/101‑1 (MQ=21)
                                                          tCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGccc                              >  1:2442045/1‑101 (MQ=21)
                                                          tCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGccc                              <  1:4154338/101‑1 (MQ=21)
                                                           cATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCg                             <  2:4222030/101‑1 (MQ=21)
                                                              cTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCg                          >  2:1159957/1‑101 (MQ=25)
                                                              cTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCg                          >  2:4164361/1‑101 (MQ=25)
                                                                tGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCgtg                        >  1:3501644/1‑101 (MQ=25)
                                                                tGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCgtg                        >  2:191748/1‑101 (MQ=25)
                                                                tGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCgtg                        >  1:1867594/1‑101 (MQ=25)
                                                                 gCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCgtgt                       >  1:1654988/1‑101 (MQ=25)
                                                                 gCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCgtgt                       >  2:2995325/1‑101 (MQ=25)
                                                                 gCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCgtgt                       >  1:3622370/1‑101 (MQ=25)
                                                                  cctcAAATAG.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGtt                      <  2:1983596/98‑1 (MQ=255)
                                                                   cGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTg                     >  2:4054662/1‑101 (MQ=25)
                                                                     cAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCa                   <  1:1210474/101‑1 (MQ=255)
                                                                     cAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCa                   >  1:2223171/1‑101 (MQ=25)
                                                                     cAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCa                   <  1:978520/101‑1 (MQ=255)
                                                                       aaTAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATc                 >  1:607924/1‑101 (MQ=255)
                                                                       aaTAT.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATc                 >  1:879041/1‑101 (MQ=255)
                                                                           t.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCgcgg             >  1:3538011/1‑101 (MQ=255)
                                                                           t.CCCGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACATCAGATCAGCCCGGCGTGTTGCATCgcgg             >  2:1574151/1‑101 (MQ=255)
                                                                             gcgggggggAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCgcggc            <  1:4188926/98‑1 (MQ=255)
                                                                               cGGGGGGAGGCTT.GCTGAAAGC.AAGACGGGGGGAAGAGC.....CCCC.CGCACAGGTCGCAAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCgcggcgg          >  2:3477870/1‑100 (MQ=255)
                                                                                 gggggAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCa        <  1:2515490/101‑1 (MQ=255)
                                                                                   gggAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAg      >  1:2578490/1‑101 (MQ=255)
                                                                                   gggAGGATT.GCTGAAAGC.AAGACGGGGG.....GCAGAG.CCCCGCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAg      <  2:1718445/101‑1 (MQ=255)
                                                                                    ggAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgc     >  2:3641000/1‑101 (MQ=255)
                                                                                    ggAGGCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAgc     >  2:1603751/1‑101 (MQ=255)
                                                                                       ggCTT.GCTGAAAGC.AAGACGGGGGGCAGAGC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGcgcg  >  1:3934848/1‑101 (MQ=255)
                                                                                                      |                                                                                             
TGGGGGCTCTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAG.CCGATGTCCTTCATCTTGCCGCAAATAT.CCCGGGGGGAGGCTT.GCTGAAAGCAAAGACGGGGG.....GC.....CCCC.CGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCG  >  NC_014034/408388‑408569

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG