Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,431 Δ1 bp 4.5% intergenic (+34/+114) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4310G→. 4.5% 16.5 ‑1.3 ‑0.0178intergenic (+34/+114)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (5/3):  ref base (63/106):  total (69/109)
Fisher's exact test strand distribution p-value = 2.63e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 192 total aligned reads displayed.

CCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.T.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA  >  NC_014034/432333‑432530
                                                                                                     |                                                                                                   
cccGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGa                                                                                                    <  2:203574/101‑1 (MQ=255)
cccGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGa                                                                                                    >  2:3257855/1‑101 (MQ=255)
  gcagcgcTGGGCACGATGGGGATAGATCCGCGCACGCTTTCGGCGGCGGCGGGCCAAGACTCAGGCCGGGCAGGAGAGAGCTGACCCCC.GCC.CTGCGGACt                                                                                                  >  2:726375/4‑101 (MQ=255)
  cGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACt                                                                                                  >  1:1397944/1‑101 (MQ=255)
      cgcTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTcccc                                                                                              <  1:2240108/101‑1 (MQ=255)
      cgcTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTcccc                                                                                              >  2:1490750/1‑101 (MQ=255)
       gatGGGCACGATGGGGAGCGATCCGCGCACGCTTGCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTccccc                                                                                             <  2:3861814/99‑1 (MQ=37)
        cTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTcccccc                                                                                            <  1:1781109/101‑1 (MQ=255)
        cTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTcccccc                                                                                            <  1:2813592/101‑1 (MQ=255)
         tGGGCACGATGGGGATCGATCCGCGCCCGCTTTCGGCGGGGGCGGGCCACGCCTGAGGCCGGGCGGGGGGGGGCGGCCCCCC.G.TCCTGCGGACTCCCCCCg                                                                                           >  2:3587263/1‑101 (MQ=37)
         tGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTccccccc                                                                                           >  2:2246242/1‑100 (MQ=255)
         tGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCg                                                                                           >  2:315115/1‑101 (MQ=255)
         tGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCg                                                                                           <  1:1265373/101‑1 (MQ=255)
         tGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCg                                                                                           <  2:3090481/101‑1 (MQ=255)
         tGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCg                                                                                           <  1:4064384/101‑1 (MQ=255)
         tGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCg                                                                                           >  1:3674532/1‑101 (MQ=255)
             cACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGat                                                                                       <  2:3438372/101‑1 (MQ=255)
               cGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGatat                                                                                     <  1:1349423/101‑1 (MQ=255)
                  tGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTg                                                                                  <  2:2005975/101‑1 (MQ=255)
                  tGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTg                                                                                  <  1:262315/101‑1 (MQ=255)
                   ggggATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCCGGGTAtttca                                                                                 >  1:863520/1‑99 (MQ=25)
                      gatcgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGc                                                                              <  1:1030510/101‑1 (MQ=255)
                       atcgatcCGCGCACGCTTTCGGCGGGGGCGGGCCACGCCTTAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGGCTCCCCCCGGGATATTTGAGGCa                                                                             <  1:351498/101‑1 (MQ=25)
                       atcgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCa                                                                             <  1:3332201/101‑1 (MQ=255)
                        tcgatcCGCGCACGCTTTCGGCGGCGGCGGNCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCaa                                                                            <  1:1170312/101‑1 (MQ=255)
                         cgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAg                                                                           <  1:2344389/101‑1 (MQ=255)
                         cgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAg                                                                           <  1:1832292/101‑1 (MQ=255)
                         cgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAg                                                                           <  1:1530272/101‑1 (MQ=255)
                         cgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAg                                                                           <  2:2750956/101‑1 (MQ=255)
                          gatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAggaccga                                                                          >  2:1050060/1‑96 (MQ=255)
                          gatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGa                                                                          >  1:1267948/1‑101 (MQ=255)
                          gatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGAGTTTTGAGGCAAGa                                                                          >  1:458088/1‑101 (MQ=25)
                            tnNGCNNACGCTTTCGGNGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGTCAAGATg                                                                        <  2:1127254/101‑1 (MQ=21)
                              cgcgCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGACATTTTCGGGCAGATGaa                                                                      >  2:857126/1‑101 (MQ=21)
                               gcgcACTCTTTCGGCGGCGGGGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGaaa                                                                     <  1:1102042/101‑1 (MQ=18)
                                 gcACGCTTTCGGCGGCGGCGGGCCACGCCGGAGGCCGGGCAGGGGGGCGCGGCCCCCCGG.C.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGc                                                                   <  1:2678140/101‑1 (MQ=21)
                                  cACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGcc                                                                  <  2:686013/101‑1 (MQ=255)
                                  cACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCCGGATATTTTAGGCAAGATGAAAGcc                                                                  >  2:1331927/1‑101 (MQ=21)
                                   aCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCg                                                                 <  1:3722350/101‑1 (MQ=255)
                                   aCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCg                                                                 <  2:2059060/101‑1 (MQ=255)
                                    cGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGa                                                                <  2:4331726/101‑1 (MQ=255)
                                      cTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATc                                                              <  1:2849999/101‑1 (MQ=255)
                                       tttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCTCCGGGGTATTTGAGGCAAGATGAAGCCCGATcc                                                             >  1:3850092/1‑101 (MQ=21)
                                        ttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCg                                                            <  1:1968169/101‑1 (MQ=255)
                                           ggcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGc                                                         >  1:2776396/1‑101 (MQ=255)
                                            gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCt                                                        <  2:941398/101‑1 (MQ=255)
                                             cggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                       <  2:2928521/101‑1 (MQ=255)
                                             cggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                       <  1:2731068/101‑1 (MQ=255)
                                              ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                                      >  2:2595712/1‑101 (MQ=255)
                                              ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                                      <  1:1214384/101‑1 (MQ=255)
                                              ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCCGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCCTc                                                      >  2:3170009/1‑101 (MQ=21)
                                                  gggggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGttt                                                  <  1:3136909/99‑1 (MQ=255)
                                                   cggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGGTTc                                                 >  2:4038378/1‑101 (MQ=255)
                                                      gCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATc                                              <  1:1879306/101‑1 (MQ=255)
                                                        cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                            <  2:1778673/101‑1 (MQ=255)
                                                        cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                            <  2:173876/101‑1 (MQ=255)
                                                        cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCCTCGTTTCATcct                                            >  2:797111/1‑99 (MQ=255)
                                                         aCGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTg                                           <  1:1916388/101‑1 (MQ=255)
                                                          cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGc                                          <  2:3170758/101‑1 (MQ=255)
                                                          cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGc                                          >  1:558702/1‑101 (MQ=255)
                                                           gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcc                                         >  2:4364611/1‑101 (MQ=255)
                                                           gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcc                                         <  1:2575464/101‑1 (MQ=255)
                                                              tGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCa                                      <  1:599427/101‑1 (MQ=255)
                                                                  gCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAata                                  <  2:2481959/101‑1 (MQ=255)
                                                                      ggacggggtgCTCTGCCCCCC.G.G.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAtt                   <  2:1564473/97‑1 (MQ=2)
                                                                       gacggggggCTCTGCCCCCC.G.G.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttg                  >  1:3903003/4‑100 (MQ=2)
                                                                        cAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGAAAGATGAAAGCCGATCCGGGCTTAGTTTCATCTTGCCCCAAATATCCCgg                            >  1:3553983/1‑101 (MQ=25)
                                                                        acggggggCTCTGCCCCCC.G.G.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttgg                 >  2:3465243/3‑99 (MQ=2)
                                                                         aGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                           >  1:2153747/1‑101 (MQ=255)
                                                                         aGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                           >  2:1951916/1‑101 (MQ=255)
                                                                          ggggggCTCTGCCCCCC.G.G.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttggcc               <  1:1418386/101‑5 (MQ=2)
                                                                          ggggggCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgggg                          <  2:458048/101‑1 (MQ=255)
                                                                          ggggggCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgggg                          <  1:1660942/101‑1 (MQ=255)
                                                                           gggggCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGCGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggggg                         >  2:3663196/1‑101 (MQ=255)
                                                                            ggggCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGCTATTTGAGGCAATACGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCTAAATATCCCGGGGGt                        >  2:3000739/1‑101 (MQ=25)
                                                                            ggggCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGt                        <  2:1267855/101‑1 (MQ=255)
                                                                            ggggCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGt                        <  1:3258087/101‑1 (MQ=255)
                                                                             gggCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTg                       <  2:593968/101‑1 (MQ=255)
                                                                               gcccTGCCCCCC.G.TCCTGCGGGCCTCCCCCGGGATAATTGAGGGAAAATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGaa                     <  2:2566367/98‑1 (MQ=25)
                                                                                cgcTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGGAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATGCCGGGGGCGAAt                    >  2:645146/1‑101 (MQ=25)
                                                                                cgcTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAt                    >  1:337882/1‑101 (MQ=255)
                                                                                cgcTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAt                    <  2:1397837/101‑1 (MQ=255)
                                                                                  cTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTTAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTTCCCCAAATATCCCCGGGGGGAAttt                  >  2:36233/1‑101 (MQ=25)
                                                                                  cTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGAAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAttt                  >  1:4091887/1‑101 (MQ=255)
                                                                                   tGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCTTCTTGCCCCAAATATCCCGGGGGGGAAtttt                 >  1:4268833/1‑101 (MQ=25)
                                                                                   tGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt                 >  2:1915128/1‑101 (MQ=255)
                                                                                   tGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt                 >  2:748745/1‑101 (MQ=255)
                                                                                    gCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTg                >  2:3067673/1‑101 (MQ=255)
                                                                                        ccc.G.TCCTGCGGACTCCCCCCGGGCTATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGgcgc            >  2:3342972/1‑101 (MQ=255)
                                                                                            cgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATACGGGCCTCGTTTCATTTTGCCCCAAATATCCCGGGGGGGGATTTTGGAGCAg          >  1:3947264/5‑101 (MQ=18)
                                                                                             gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGTCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc         >  2:4209462/4‑101 (MQ=255)
                                                                                              tccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGcc        <  1:4379286/99‑1 (MQ=255)
                                                                                              tccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGcc        <  1:4015698/99‑1 (MQ=255)
                                                                                               ccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa       >  1:3461589/2‑101 (MQ=255)
                                                                                                cTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaa      >  2:3699599/1‑101 (MQ=255)
                                                                                                 tGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaa     >  1:1386625/1‑101 (MQ=255)
                                                                                                   cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAag   <  2:3966551/101‑1 (MQ=255)
                                                                                                   cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAag   <  1:2162705/101‑1 (MQ=255)
                                                                                                    ggACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAaga  <  1:2020531/101‑1 (MQ=255)
                                                                                                    ggACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAaga  <  1:748825/101‑1 (MQ=255)
                                                                                                     |                                                                                                   
CCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.T.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGA  >  NC_014034/432333‑432530

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG