Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,432 Δ1 bp 4.5% intergenic (+35/+113) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4320A→. 4.5% 15.8 ‑1.3 ‑0.4178intergenic (+35/+113)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (5/3):  ref base (64/106):  total (69/109)
Fisher's exact test strand distribution p-value = 2.64e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.02e-01

Only 100 of 193 total aligned reads displayed.

CCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.T.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGG  >  NC_014034/432333‑432532
                                                                                                      |                                                                                                    
  gcagcgcTGGGCACGATGGGGATAGATCCGCGCACGCTTTCGGCGGCGGCGGGCCAAGACTCAGGCCGGGCAGGAGAGAGCTGACCCCC.GCC.CTGCGGACt                                                                                                    >  2:726375/4‑101 (MQ=255)
  cGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACt                                                                                                    >  1:1397944/1‑101 (MQ=255)
   gggCGGTGGGCACGATGGGGATGGATCCGCGCGCGCTTGCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTc                                                                                                   <  2:1454362/101‑1 (MQ=37)
   gggCGCGGGGCACGAGGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCTGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTc                                                                                                   <  2:10693/101‑1 (MQ=37)
    ggCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTcc                                                                                                  <  1:3893579/101‑1 (MQ=255)
      cgcTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTcccc                                                                                                <  1:2240108/101‑1 (MQ=255)
        cTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTcccccc                                                                                              <  1:1781109/101‑1 (MQ=255)
         tGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCg                                                                                             <  2:3090481/101‑1 (MQ=255)
         tGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCg                                                                                             >  2:2162566/1‑101 (MQ=255)
         tGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCg                                                                                             <  1:1265373/101‑1 (MQ=255)
         tGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCg                                                                                             >  2:315115/1‑101 (MQ=255)
         tGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCg                                                                                             >  1:3674532/1‑101 (MQ=255)
         tGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCg                                                                                             >  2:3640517/1‑101 (MQ=255)
            gCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGa                                                                                          >  2:3309019/1‑101 (MQ=255)
               cGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGatat                                                                                       <  1:1349423/101‑1 (MQ=255)
                gATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATAtt                                                                                      <  2:303280/101‑1 (MQ=255)
                gATGGGGATCGATCCGCGCACGCTTTCGGCGCCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCcggggatt                                                                                      >  1:3955311/1‑96 (MQ=25)
                  tGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTg                                                                                    <  2:2005975/101‑1 (MQ=255)
                  tGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTg                                                                                    <  1:262315/101‑1 (MQ=255)
                   ggggATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCCGGGTAtttca                                                                                   >  1:863520/1‑99 (MQ=25)
                      gatcgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGc                                                                                <  1:1030510/101‑1 (MQ=255)
                       atcgatcCGCGCACGCTTTCGGCGGGGGCGGGCCACGCCTTAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGGCTCCCCCCGGGATATTTGAGGCa                                                                               <  1:351498/101‑1 (MQ=25)
                       atcgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCa                                                                               <  1:3332201/101‑1 (MQ=255)
                        tcgatcCGCGCACGCTTTCGGCGGCGGCGGNCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCaa                                                                              <  1:1170312/101‑1 (MQ=255)
                         cgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAg                                                                             <  1:2344389/101‑1 (MQ=255)
                         cgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAg                                                                             <  1:1530272/101‑1 (MQ=255)
                          gatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGa                                                                            >  1:1267948/1‑101 (MQ=255)
                          gatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGAGTTTTGAGGCAAGa                                                                            >  1:458088/1‑101 (MQ=25)
                           atcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGAt                                                                           <  2:3698868/101‑1 (MQ=255)
                            tcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATg                                                                          <  2:3707889/101‑1 (MQ=255)
                              cgcgCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGaa                                                                        >  2:3491469/1‑101 (MQ=255)
                              cgcgCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGACATTTTCGGGCAGATGaa                                                                        >  2:857126/1‑101 (MQ=21)
                               gcgcACTCTTTCGGCGGCGGGGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGaaa                                                                       <  1:1102042/101‑1 (MQ=18)
                               gcgcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGaaa                                                                       >  1:2826810/1‑101 (MQ=255)
                                cgcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAg                                                                      <  1:4073935/101‑1 (MQ=255)
                                 gcACGCTTTCGGCGGCGGCGGGCCACGCCGGAGGCCGGGCAGGGGGGCGCGGCCCCCCGG.C.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGc                                                                     <  1:2678140/101‑1 (MQ=21)
                                  cACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGcc                                                                    <  2:686013/101‑1 (MQ=255)
                                   aCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCg                                                                   <  1:3722350/101‑1 (MQ=255)
                                    cGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGa                                                                  <  2:4331726/101‑1 (MQ=255)
                                     gCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGAt                                                                 <  2:3605966/101‑1 (MQ=255)
                                      cTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATc                                                                <  2:2507141/101‑1 (MQ=255)
                                        ttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCg                                                              <  2:1370921/101‑1 (MQ=255)
                                        ttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCg                                                              <  1:1968169/101‑1 (MQ=255)
                                            gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCt                                                          <  2:941398/101‑1 (MQ=255)
                                             cgtcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                         <  2:2571579/98‑1 (MQ=255)
                                             cggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                         <  1:2731068/101‑1 (MQ=255)
                                             cggcggcggGCCACGCCTGAGGCCGGCAAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                         <  2:1710840/101‑1 (MQ=21)
                                              ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                                        <  1:1214384/101‑1 (MQ=255)
                                              ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                                        <  2:2773287/101‑1 (MQ=255)
                                              ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCCGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCCTc                                                        >  2:3170009/1‑101 (MQ=21)
                                               gcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCg                                                       <  2:1134490/101‑1 (MQ=255)
                                                  gggggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGttt                                                    <  1:3136909/99‑1 (MQ=255)
                                                     ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                 >  2:3436232/1‑101 (MQ=255)
                                                      gCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATc                                                <  2:2054554/101‑1 (MQ=255)
                                                        cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                              >  2:3922964/1‑101 (MQ=255)
                                                        cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                              <  2:173876/101‑1 (MQ=255)
                                                        cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                              <  2:1778673/101‑1 (MQ=255)
                                                        cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCCTCGTTTCATcct                                              >  2:797111/1‑99 (MQ=255)
                                                         aCGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTg                                             <  1:1916388/101‑1 (MQ=255)
                                                         aCGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTg                                             <  1:1877662/101‑1 (MQ=255)
                                                          cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGc                                            >  1:558702/1‑101 (MQ=255)
                                                           gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcc                                           <  1:2575464/101‑1 (MQ=255)
                                                           gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcc                                           >  2:4364611/1‑101 (MQ=255)
                                                              tGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCa                                        <  1:599427/101‑1 (MQ=255)
                                                                 ggCCGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAat                                     <  2:2979145/101‑1 (MQ=255)
                                                                   ccGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAatat                                   <  2:2311039/101‑1 (MQ=255)
                                                                    cGGGCAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATc                                  <  2:3175109/101‑1 (MQ=255)
                                                                      ggacggggggCTCTGCCCCCC.G.G.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAtt                     >  1:1895554/5‑101 (MQ=2)
                                                                        cAGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGAAAGATGAAAGCCGATCCGGGCTTAGTTTCATCTTGCCCCAAATATCCCgg                              >  1:3553983/1‑101 (MQ=25)
                                                                         cggggggCTCTGCCCCCC.G.G.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttggc                  <  1:2878129/100‑4 (MQ=2)
                                                                         aGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                             >  1:2153747/1‑101 (MQ=255)
                                                                         aGGGGGGCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                             >  2:1951916/1‑101 (MQ=255)
                                                                          ggggggCTCTGCCCCCC.G.G.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttggcc                 <  1:1418386/101‑5 (MQ=2)
                                                                          ggggggCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgggg                            <  2:458048/101‑1 (MQ=255)
                                                                          ggggggCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgggg                            <  1:1660942/101‑1 (MQ=255)
                                                                            ggggCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGt                          <  1:3258087/101‑1 (MQ=255)
                                                                             gggCGCTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTg                         <  2:593968/101‑1 (MQ=255)
                                                                               gcgcTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGaa                       <  1:4038635/101‑1 (MQ=255)
                                                                                cgcTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGGAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATGCCGGGGGCGAAt                      >  2:645146/1‑101 (MQ=25)
                                                                                cgcTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAt                      >  1:337882/1‑101 (MQ=255)
                                                                                cgcTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAt                      >  2:127379/1‑101 (MQ=255)
                                                                                  cTGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGAAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAttt                    >  1:4091887/1‑101 (MQ=255)
                                                                                   tGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCTTCTTGCCCCAAATATCCCGGGGGGGAAtttt                   >  1:4268833/1‑101 (MQ=25)
                                                                                   tGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt                   >  2:748745/1‑101 (MQ=255)
                                                                                   tGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt                   >  2:1915128/1‑101 (MQ=255)
                                                                                   tGCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt                   >  1:2809354/1‑101 (MQ=255)
                                                                                    gCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTg                  >  2:3455892/1‑101 (MQ=255)
                                                                                    gCCCCCC.G.TCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTg                  >  2:3067673/1‑101 (MQ=255)
                                                                                           ccgtccTGCGGACTCCCCCCGGGATATTTTAGGCAAAATGAAAACCGAACCGGGGTTCGTTTCATCTTGCCCCCAAAATCCCCGGGGGGAATTTTgtggcc             >  2:1539672/6‑96 (MQ=18)
                                                                                           ccgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCa             <  2:2659785/96‑1 (MQ=255)
                                                                                             gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGTCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc           >  2:4209462/4‑101 (MQ=255)
                                                                                              tccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGcc          <  1:4379286/99‑1 (MQ=255)
                                                                                               ccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa         >  1:3461589/2‑101 (MQ=255)
                                                                                                 tGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaa       >  1:1386625/1‑101 (MQ=255)
                                                                                                  gCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaaa      <  1:3923218/101‑1 (MQ=255)
                                                                                                   cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAag     <  2:3966551/101‑1 (MQ=255)
                                                                                                   cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAag     <  1:2162705/101‑1 (MQ=255)
                                                                                                    ggACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAaga    <  1:748825/101‑1 (MQ=255)
                                                                                                    ggACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAaga    <  1:2020531/101‑1 (MQ=255)
                                                                                                      aCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg  >  2:399851/1‑101 (MQ=255)
                                                                                                      |                                                                                                    
CCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.G.T.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGG  >  NC_014034/432333‑432532

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG