Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,465 Δ1 bp 4.8% intergenic (+68/+80) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4650C→. 4.8% 17.4 ‑0.8 ‑0.2168intergenic (+68/+80)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (5/3):  ref base (70/89):  total (76/92)
Fisher's exact test strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.81e-01

Only 100 of 188 total aligned reads displayed.

GCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGC  >  NC_014034/432366‑432565
                                                                                                    |                                                                                                     
 cACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGcc                                                                                                      <  1:4299844/101‑1 (MQ=255)
 cACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCCGGATATTTTAGGCAAGATGAAAGcc                                                                                                      >  2:1331927/1‑101 (MQ=21)
  aCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCg                                                                                                     <  2:2059060/101‑1 (MQ=255)
  aCGCCTCCGGCGGCGGCGGGCCGCGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCg                                                                                                     <  1:3353045/101‑1 (MQ=18)
   cGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGa                                                                                                    <  2:4331726/101‑1 (MQ=255)
    gCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGAt                                                                                                   <  2:3605966/101‑1 (MQ=255)
      tttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCTCCGGGGTATTTGAGGCAAGATGAAGCCCGATcc                                                                                                 >  1:3850092/1‑101 (MQ=21)
       ttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCg                                                                                                <  1:1968169/101‑1 (MQ=255)
          ggcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGc                                                                                             >  1:2776396/1‑101 (MQ=255)
           gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCt                                                                                            <  2:941398/101‑1 (MQ=255)
            cggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                                                           <  2:2928521/101‑1 (MQ=255)
            cggcggcggGCCACGCCTGAGGCCGGCAAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                                                           <  2:1710840/101‑1 (MQ=21)
             ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                                                                          >  2:2595712/1‑101 (MQ=255)
              gcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCg                                                                                         <  2:1134490/101‑1 (MQ=255)
                ggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGtt                                                                                       <  1:2047272/101‑1 (MQ=255)
                 gggggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGttt                                                                                      <  1:3136909/99‑1 (MQ=255)
                    ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                                   >  2:3436232/1‑101 (MQ=255)
                      ccACGCCTGAGGCCGGGGAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCt                                                                                 <  1:1267872/101‑1 (MQ=255)
                       cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                                <  2:1778673/101‑1 (MQ=255)
                       cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                                <  2:173876/101‑1 (MQ=255)
                       cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                                >  1:2659957/1‑101 (MQ=255)
                        aCGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTg                                                                               <  1:1877662/101‑1 (MQ=255)
                         cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGc                                                                              >  1:558702/1‑101 (MQ=255)
                         cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGc                                                                              <  1:2370166/101‑1 (MQ=255)
                         cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGc                                                                              <  2:3170758/101‑1 (MQ=255)
                          gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcc                                                                             >  2:4364611/1‑101 (MQ=255)
                          gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcc                                                                             <  1:2575464/101‑1 (MQ=255)
                            cTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGTATTTGAGGTAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                                                                           >  1:3680842/1‑101 (MQ=21)
                                ggCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAat                                                                       <  2:2979145/101‑1 (MQ=255)
                                 gCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAata                                                                      <  2:2481959/101‑1 (MQ=255)
                                  ccGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAatat                                                                     <  2:2311039/101‑1 (MQ=255)
                                    gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATcc                                                                   <  1:2715247/101‑1 (MQ=255)
                                     ggacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTG.AAtt                                                      >  1:1895554/5‑101 (MQ=2)
                                      gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTG.AAttg                                                     >  1:3903003/4‑100 (MQ=2)
                                      gCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCg                                                                 <  1:1391283/101‑1 (MQ=255)
                                       cAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGAAAGATGAAAGCCGATCCGGGCTTAGTTTCATCTTGCCCCAAATATCCCgg                                                                >  1:3553983/1‑101 (MQ=25)
                                        cggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTG.AAttggc                                                   <  1:2878129/100‑4 (MQ=2)
                                        aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                                                               >  2:1951916/1‑101 (MQ=255)
                                        aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                                                               >  1:2153747/1‑101 (MQ=255)
                                         ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgggg                                                              <  1:1660942/101‑1 (MQ=255)
                                          gggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGCGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggggg                                                             >  2:3663196/1‑101 (MQ=255)
                                           ggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGt                                                            <  1:3258087/101‑1 (MQ=255)
                                            gggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTg                                                           <  1:3436471/101‑1 (MQ=255)
                                            gggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTg                                                           <  2:593968/101‑1 (MQ=255)
                                              gcccTGCCCCCCGTCCTGCGGGCCTCCCCCGGGATAATTGAGGGAAAATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.aa                                                        <  2:2566367/98‑1 (MQ=25)
                                               cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGGAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATGCCGGGGGCG.AAt                                                       >  2:645146/1‑101 (MQ=25)
                                               cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAt                                                       >  2:127379/1‑101 (MQ=255)
                                                 cTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAttt                                                     <  2:307592/101‑1 (MQ=255)
                                                 cTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGAAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAttt                                                     >  1:4091887/1‑101 (MQ=255)
                                                  tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAtttt                                                    >  2:1915128/1‑101 (MQ=255)
                                                  tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAtttt                                                    >  2:748745/1‑101 (MQ=255)
                                                  tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCAGGACTTCGTTTCATCTTGCCCCAAATATCCCCGGGGTG.AAtttt                                                    >  2:4412183/1‑101 (MQ=25)
                                                   gCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTg                                                   >  2:3067673/1‑101 (MQ=255)
                                                     cccccGTCCTGCGGACTCCCCCCGGGATATTTTAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTTATCTTGCCCCCAATATTCCGGGGGGGAAATTTTgg                                                  >  2:3130228/1‑101 (MQ=21)
                                                      ccccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGgcg                                                <  1:1370521/101‑1 (MQ=255)
                                                        ccgtccTGCGGACTCCCCCCGGGATATTTTAGGCAAAATGAAAACCGAACCGGGGTTCGTTTCATCTTGCCCCCAAAATCCCCGGGGGG.AATTTTgtggcc                                              >  2:1539672/6‑96 (MQ=18)
                                                         cgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAg                                             <  1:3467986/97‑1 (MQ=255)
                                                         cgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAg                                             >  1:455790/5‑101 (MQ=255)
                                                         cgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATACGGGCCTCGTTTCATTTTGCCCCAAATATCCCGGGGGGG.GATTTTGGAGCAg                                             >  1:3947264/5‑101 (MQ=18)
                                                          gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGTCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGc                                            >  2:4209462/4‑101 (MQ=255)
                                                           tccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGcc                                           <  1:4015698/99‑1 (MQ=255)
                                                             cTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCaa                                         <  2:723720/101‑1 (MQ=255)
                                                                cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAag                                      <  2:3966551/101‑1 (MQ=255)
                                                                 ggACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAaga                                     <  1:2020531/101‑1 (MQ=255)
                                                                 ggACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAaga                                     <  1:748825/101‑1 (MQ=255)
                                                                   aCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAgg                                   >  2:399851/1‑101 (MQ=255)
                                                                    cTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAggg                                  >  2:1036440/1‑101 (MQ=255)
                                                                    cTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAggg                                  <  2:3314560/101‑1 (MQ=255)
                                                                     tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGTCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAgggg                                 <  2:3680589/101‑1 (MQ=255)
                                                                     tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAgggg                                 >  2:2668008/1‑101 (MQ=255)
                                                                        ccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCa                              <  1:4364882/101‑1 (MQ=255)
                                                                         cccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCat                             >  1:1123477/1‑100 (MQ=255)
                                                                         cccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAg                             <  2:2776206/101‑1 (MQ=255)
                                                                          ccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGGG.AATTTTGGCGCAGCCAAAAGAGGGGACAgc                            >  1:3726286/1‑101 (MQ=255)
                                                                          ccGGGATATTTGAGGCAAGATGAAAGCCGATCAGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAgc                            >  1:669975/1‑101 (MQ=255)
                                                                           cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                           <  1:3640770/101‑1 (MQ=255)
                                                                           cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                           >  2:1422484/1‑101 (MQ=255)
                                                                            gggATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAgcgc                          >  1:2225443/1‑101 (MQ=255)
                                                                              gATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                        >  2:2771542/1‑101 (MQ=255)
                                                                                tatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                      >  1:3683254/1‑101 (MQ=255)
                                                                                tatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                      <  2:3763654/101‑1 (MQ=255)
                                                                                 atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                     >  1:1211740/1‑101 (MQ=255)
                                                                                 atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                     >  2:201369/1‑101 (MQ=255)
                                                                                 atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                     >  1:3175898/1‑101 (MQ=255)
                                                                                  tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                    >  2:1853137/1‑101 (MQ=255)
                                                                                   ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                   <  2:1386528/101‑1 (MQ=255)
                                                                                    tGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATc                  >  1:3586026/1‑101 (MQ=255)
                                                                                    tGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCCATc                  >  1:2845410/1‑101 (MQ=255)
                                                                                       ggCAAGCTGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc               <  1:736439/101‑1 (MQ=255)
                                                                                       ggCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc               <  2:4268564/101‑1 (MQ=255)
                                                                                        gCAAGATGAAAGCCGGTCGGGGCTGCGTTTCATCTTGCCCCAAATATCCCGGGGGTT.ATTTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg              <  2:669907/101‑1 (MQ=25)
                                                                                        gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg              >  1:911118/1‑101 (MQ=255)
                                                                                         cAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgc             <  2:3461349/101‑1 (MQ=255)
                                                                                           aGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcgg           >  1:150635/1‑101 (MQ=255)
                                                                                              tGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgc        >  2:1599185/1‑101 (MQ=255)
                                                                                               gAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg       >  1:1472402/1‑101 (MQ=255)
                                                                                                 aaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc     <  1:1050148/101‑1 (MQ=255)
                                                                                                    ccGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggc  <  2:1123079/101‑1 (MQ=255)
                                                                                                    ccGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggc  >  1:2308350/1‑101 (MQ=255)
                                                                                                    ccGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggc  <  2:863435/101‑1 (MQ=255)
                                                                                                    |                                                                                                     
GCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGC  >  NC_014034/432366‑432565

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG