Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,466 Δ1 bp 4.8% intergenic (+69/+79) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4660C→. 4.8% 17.4 ‑0.8 ‑0.1168intergenic (+69/+79)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (5/3):  ref base (69/89):  total (76/92)
Fisher's exact test strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.44e-01

Only 100 of 189 total aligned reads displayed.

CACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCG  >  NC_014034/432367‑432566
                                                                                                    |                                                                                                     
cACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGcc                                                                                                       <  1:4299844/101‑1 (MQ=255)
cACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCCGGATATTTTAGGCAAGATGAAAGcc                                                                                                       >  2:1331927/1‑101 (MQ=21)
 aCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCg                                                                                                      <  1:3722350/101‑1 (MQ=255)
  cGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGa                                                                                                     <  2:4331726/101‑1 (MQ=255)
         ggcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGc                                                                                              >  1:2776396/1‑101 (MQ=255)
          gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCt                                                                                             <  2:941398/101‑1 (MQ=255)
            ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                                                                           >  2:2595712/1‑101 (MQ=255)
             gcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCg                                                                                          <  2:1134490/101‑1 (MQ=255)
                gggggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGttt                                                                                       <  1:3136909/99‑1 (MQ=255)
                   ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                                    >  2:3436232/1‑101 (MQ=255)
                    gCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATc                                                                                   <  1:1879306/101‑1 (MQ=255)
                    gCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATc                                                                                   <  2:2054554/101‑1 (MQ=255)
                     ccACGCCTGAGGCCGGGGAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCt                                                                                  <  1:1267872/101‑1 (MQ=255)
                      cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                                 <  2:173876/101‑1 (MQ=255)
                      cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                                 <  2:1778673/101‑1 (MQ=255)
                       aCGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTg                                                                                <  1:1916388/101‑1 (MQ=255)
                        cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGc                                                                               <  2:3170758/101‑1 (MQ=255)
                        cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGc                                                                               <  1:2370166/101‑1 (MQ=255)
                         gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcc                                                                              <  1:2575464/101‑1 (MQ=255)
                           cTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGTATTTGAGGTAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                                                                            >  1:3680842/1‑101 (MQ=21)
                           cTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                                                                            <  1:2609731/101‑1 (MQ=255)
                           cTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                                                                            <  1:719163/101‑1 (MQ=255)
                                gCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAata                                                                       <  2:2481959/101‑1 (MQ=255)
                                   gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATcc                                                                    <  1:2715247/101‑1 (MQ=255)
                                    ggacggggtgCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTG.AAtt                                                       <  2:1564473/97‑1 (MQ=2)
                                    ggacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTG.AAtt                                                       >  1:1895554/5‑101 (MQ=2)
                                     gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTG.AAttg                                                      >  1:3903003/4‑100 (MQ=2)
                                     gCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCg                                                                  <  1:1391283/101‑1 (MQ=255)
                                     gCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCg                                                                  <  1:2000895/101‑1 (MQ=255)
                                      cAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGAAAGATGAAAGCCGATCCGGGCTTAGTTTCATCTTGCCCCAAATATCCCgg                                                                 >  1:3553983/1‑101 (MQ=25)
                                      acggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTG.AAttgg                                                     >  2:3465243/3‑99 (MQ=2)
                                       cggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTG.AAttggc                                                    <  1:2878129/100‑4 (MQ=2)
                                       aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                                                                >  2:1951916/1‑101 (MQ=255)
                                        ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgggg                                                               <  1:1660942/101‑1 (MQ=255)
                                          ggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGCTATTTGAGGCAATACGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCTAAATATCCCGGGGGt                                                             >  2:3000739/1‑101 (MQ=25)
                                           gggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTg                                                            <  1:3436471/101‑1 (MQ=255)
                                           gggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTg                                                            <  2:593968/101‑1 (MQ=255)
                                            ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.a                                                          <  2:1963676/101‑1 (MQ=255)
                                             gcccTGCCCCCCGTCCTGCGGGCCTCCCCCGGGATAATTGAGGGAAAATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.aa                                                         <  2:2566367/98‑1 (MQ=25)
                                              cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGGAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATGCCGGGGGCG.AAt                                                        >  2:645146/1‑101 (MQ=25)
                                              cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAt                                                        <  2:1397837/101‑1 (MQ=255)
                                              cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAt                                                        >  2:127379/1‑101 (MQ=255)
                                              cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAt                                                        >  1:337882/1‑101 (MQ=255)
                                                cTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTTAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTTCCCCAAATATCCCCGGGGGG.AAttt                                                      >  2:36233/1‑101 (MQ=25)
                                                cTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAttt                                                      <  2:307592/101‑1 (MQ=255)
                                                cTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGAAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAttt                                                      >  1:4091887/1‑101 (MQ=255)
                                                 tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAtttt                                                     >  2:748745/1‑101 (MQ=255)
                                                 tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAtttt                                                     >  2:1915128/1‑101 (MQ=255)
                                                 tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCAGGACTTCGTTTCATCTTGCCCCAAATATCCCCGGGGTG.AAtttt                                                     >  2:4412183/1‑101 (MQ=25)
                                                 cgCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAtttt                                                     <  1:2284194/100‑1 (MQ=255)
                                                    cccccGTCCTGCGGACTCCCCCCGGGATATTTTAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTTATCTTGCCCCCAATATTCCGGGGGGGAAATTTTgg                                                   >  2:3130228/1‑101 (MQ=21)
                                                     ccccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGgcg                                                 <  1:1370521/101‑1 (MQ=255)
                                                        cgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAg                                              <  1:3467986/97‑1 (MQ=255)
                                                        cgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATACGGGCCTCGTTTCATTTTGCCCCAAATATCCCGGGGGGG.GATTTTGGAGCAg                                              >  1:3947264/5‑101 (MQ=18)
                                                         gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGTCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGc                                             >  2:4209462/4‑101 (MQ=255)
                                                          tccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGcc                                            <  1:4379286/99‑1 (MQ=255)
                                                          tccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGcc                                            <  1:4015698/99‑1 (MQ=255)
                                                            cTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCaa                                          <  2:723720/101‑1 (MQ=255)
                                                               cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAag                                       <  2:3966551/101‑1 (MQ=255)
                                                               cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAag                                       <  1:2162705/101‑1 (MQ=255)
                                                                  aCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAgg                                    >  2:399851/1‑101 (MQ=255)
                                                                   cTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAggg                                   <  2:3314560/101‑1 (MQ=255)
                                                                    tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGTCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAgggg                                  <  2:3680589/101‑1 (MQ=255)
                                                                    tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAgggg                                  >  2:2668008/1‑101 (MQ=255)
                                                                    tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAgggg                                  >  1:747322/1‑101 (MQ=255)
                                                                    tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAgggg                                  >  2:4361890/1‑101 (MQ=255)
                                                                    gccccccGGAATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAgggg                                  <  2:2826603/100‑1 (MQ=255)
                                                                      cccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGc                                >  1:951457/1‑101 (MQ=255)
                                                                        cccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCat                              >  1:1123477/1‑100 (MQ=255)
                                                                        cccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAg                              <  2:2776206/101‑1 (MQ=255)
                                                                         ccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAgc                             <  1:478951/101‑1 (MQ=255)
                                                                         ccGGGATATTTGAGGCAAGATGAAAGCCGATCAGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAgc                             >  1:669975/1‑101 (MQ=255)
                                                                          cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                            <  1:3640770/101‑1 (MQ=255)
                                                                          cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                            >  2:1493328/1‑101 (MQ=255)
                                                                          cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAccg                            <  2:2949293/101‑1 (MQ=255)
                                                                           gggATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAgcgc                           >  2:1119944/1‑101 (MQ=255)
                                                                             gATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                         >  2:2771542/1‑101 (MQ=255)
                                                                               tatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                       <  2:3763654/101‑1 (MQ=255)
                                                                                atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                      >  1:1211740/1‑101 (MQ=255)
                                                                                atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                      >  1:3175898/1‑101 (MQ=255)
                                                                                 tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                     >  2:1853137/1‑101 (MQ=255)
                                                                                 tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                     >  1:2130728/1‑101 (MQ=255)
                                                                                  ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                    <  2:1386528/101‑1 (MQ=255)
                                                                                  ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                    <  1:3309251/101‑1 (MQ=255)
                                                                                   tGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATc                   >  1:3586026/1‑101 (MQ=255)
                                                                                   tGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATc                   >  1:3869792/1‑101 (MQ=255)
                                                                                   tGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCCATc                   >  1:2845410/1‑101 (MQ=255)
                                                                                    gAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcc                  >  1:2033703/1‑101 (MQ=255)
                                                                                     aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc                 >  1:3760642/1‑101 (MQ=255)
                                                                                      ggCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc                <  2:4268564/101‑1 (MQ=255)
                                                                                       gCAAGATGAAAGCCGGTCGGGGCTGCGTTTCATCTTGCCCCAAATATCCCGGGGGTT.ATTTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg               <  2:669907/101‑1 (MQ=25)
                                                                                       gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg               <  2:2077529/101‑1 (MQ=255)
                                                                                        cAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgc              <  2:3461349/101‑1 (MQ=255)
                                                                                        cAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgc              >  2:1616172/1‑101 (MQ=255)
                                                                                          aGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcgg            >  1:150635/1‑101 (MQ=255)
                                                                                           gATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggc           <  1:769328/101‑1 (MQ=255)
                                                                                              gAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg        >  1:1472402/1‑101 (MQ=255)
                                                                                                aaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc      <  1:1050148/101‑1 (MQ=255)
                                                                                                   ccGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggc   <  2:863435/101‑1 (MQ=255)
                                                                                                    cGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcg  <  1:59695/101‑1 (MQ=255)
                                                                                                    |                                                                                                     
CACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCG  >  NC_014034/432367‑432566

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG