Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,468 Δ1 bp 4.7% intergenic (+71/+77) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4680A→. 4.7% 16.4 ‑0.7 ‑0.2169intergenic (+71/+77)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (5/3):  ref base (74/87):  total (79/90)
Fisher's exact test strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.71e-01

Only 100 of 185 total aligned reads displayed.

CGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.C.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCG  >  NC_014034/432369‑432568
                                                                                                     |                                                                                                     
  cTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATc                                                                                                     <  1:2849999/101‑1 (MQ=255)
    ttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCg                                                                                                   <  2:1370921/101‑1 (MQ=255)
       ggcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGc                                                                                                >  1:2776396/1‑101 (MQ=255)
        gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCt                                                                                               <  2:941398/101‑1 (MQ=255)
         cggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                                                              <  2:2928521/101‑1 (MQ=255)
          ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                                                                             <  2:1791387/101‑1 (MQ=255)
          ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                                                                             >  2:2595712/1‑101 (MQ=255)
             ggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGtt                                                                                          <  1:2047272/101‑1 (MQ=255)
              gggggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGttt                                                                                         <  1:3136909/99‑1 (MQ=255)
               cggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGGTTc                                                                                        >  2:4038378/1‑101 (MQ=255)
                 ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                                      >  2:3436232/1‑101 (MQ=255)
                  gCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATc                                                                                     <  2:2054554/101‑1 (MQ=255)
                   ccACGCCTGAGGCCGGGGAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCt                                                                                    <  1:1267872/101‑1 (MQ=255)
                    cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                                   <  2:173876/101‑1 (MQ=255)
                    cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                                   >  1:2659957/1‑101 (MQ=255)
                    cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCtt                                                                                   <  2:1778673/101‑1 (MQ=255)
                    cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCAGATCCGGGCTTCGTTTTACCtt                                                                                   >  2:59682/1‑101 (MQ=18)
                     aCGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTg                                                                                  <  1:1916388/101‑1 (MQ=255)
                     aCGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTg                                                                                  <  1:1877662/101‑1 (MQ=255)
                      cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGc                                                                                 >  1:558702/1‑101 (MQ=255)
                      cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGc                                                                                 <  1:2370166/101‑1 (MQ=255)
                      cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGc                                                                                 <  2:3170758/101‑1 (MQ=255)
                       gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcc                                                                                >  2:3974588/1‑101 (MQ=255)
                       gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcc                                                                                <  1:2575464/101‑1 (MQ=255)
                         cTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGcccc                                                                              <  1:719163/101‑1 (MQ=255)
                          tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCa                                                                             <  1:599427/101‑1 (MQ=255)
                              gCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAata                                                                         <  2:2481959/101‑1 (MQ=255)
                               ccGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAatat                                                                        <  2:2311039/101‑1 (MQ=255)
                                 gggCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATcc                                                                      <  1:2715247/101‑1 (MQ=255)
                                  ggacggggtgCTCTGCCCCCCGG.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTG.AAtt                                                         <  2:1564473/97‑1 (MQ=2)
                                  ggacggggggCTCTGCCCCCCGG.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTG.AAtt                                                         >  1:1895554/5‑101 (MQ=2)
                                   gCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCg                                                                    <  1:1391283/101‑1 (MQ=255)
                                   gCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCg                                                                    <  1:2000895/101‑1 (MQ=255)
                                    cAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGAAAGATGAAAGCCGATCCGGGCTTAGTTTCATCTTGCCCCAAATATCCCgg                                                                   >  1:3553983/1‑101 (MQ=25)
                                    acggggggCTCTGCCCCCCGG.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTG.AAttgg                                                       >  2:3465243/3‑99 (MQ=2)
                                     aGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                                                                  >  1:2153747/1‑101 (MQ=255)
                                     aGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                                                                  <  2:1915454/101‑1 (MQ=255)
                                        ggggCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGCTATTTGAGGCAATACGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCTAAATATCCCGGGGGt                                                               >  2:3000739/1‑101 (MQ=25)
                                        ggggCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGt                                                               <  1:3258087/101‑1 (MQ=255)
                                        ggggCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGt                                                               <  2:1267855/101‑1 (MQ=255)
                                         gggCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTg                                                              <  2:593968/101‑1 (MQ=255)
                                          ggCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.a                                                            <  2:1963676/101‑1 (MQ=255)
                                          ggCGCTGCCCCCCGT.CTTGCGGCCGCCCCCCGGCATATTTGAGGCAGGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.a                                                            <  2:4257360/101‑1 (MQ=25)
                                           gcgcTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.aa                                                           <  1:4038635/101‑1 (MQ=255)
                                           gcccTGCCCCCCGTCC.TGCGGGCCTCCCCCGGGATAATTGAGGGAAAATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.aa                                                           <  2:2566367/98‑1 (MQ=25)
                                            cgcTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAt                                                          <  2:1397837/101‑1 (MQ=255)
                                            cgcTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAt                                                          >  1:337882/1‑101 (MQ=255)
                                              cTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTTAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTTCCCCAAATATCCCCGGGGGG.AAttt                                                        >  2:36233/1‑101 (MQ=25)
                                              cTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAttt                                                        <  2:307592/101‑1 (MQ=255)
                                              cTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGAAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAttt                                                        >  1:4091887/1‑101 (MQ=255)
                                               tGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAtttt                                                       >  2:748745/1‑101 (MQ=255)
                                               tGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAtttt                                                       <  1:1490855/101‑1 (MQ=255)
                                               tGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCAGGACTTCGTTTCATCTTGCCCCAAATATCCCCGGGGTG.AAtttt                                                       >  2:4412183/1‑101 (MQ=25)
                                               cgCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AAtttt                                                       <  1:2284194/100‑1 (MQ=255)
                                                gCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTg                                                      >  2:3455892/1‑101 (MQ=255)
                                                  cccccGTCC.TGCGGACTCCCCCCGGGATATTTTAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTTATCTTGCCCCCAATATTCCGGGGGGGAAATTTTgg                                                     >  2:3130228/1‑101 (MQ=21)
                                                   ccccGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGgcg                                                   <  1:1370521/101‑1 (MQ=255)
                                                     ccgtcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCa                                                 <  2:667298/96‑1 (MQ=255)
                                                      cgtcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAg                                                >  1:455790/5‑101 (MQ=255)
                                                         cc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCa                                             >  1:3461589/2‑101 (MQ=255)
                                                          c.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCaa                                            <  2:723720/101‑1 (MQ=255)
                                                             gCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCaaaa                                          <  1:3923218/101‑1 (MQ=255)
                                                              cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAag                                         <  2:3966551/101‑1 (MQ=255)
                                                               ggACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAaga                                        <  1:748825/101‑1 (MQ=255)
                                                               ggACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAaga                                        <  1:315150/101‑1 (MQ=255)
                                                                  cTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAggg                                     <  2:3314560/101‑1 (MQ=255)
                                                                  cTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAggg                                     >  2:1036440/1‑101 (MQ=255)
                                                                   tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGTCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAgggg                                    <  2:3680589/101‑1 (MQ=255)
                                                                   tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAgggg                                    >  2:4361890/1‑101 (MQ=255)
                                                                   tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAgggg                                    >  2:2668008/1‑101 (MQ=255)
                                                                     cccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGc                                  <  1:3645813/101‑1 (MQ=255)
                                                                      ccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCa                                 <  1:4364882/101‑1 (MQ=255)
                                                                       cccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCat                                >  1:1123477/1‑100 (MQ=255)
                                                                       cccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAg                                <  2:2776206/101‑1 (MQ=255)
                                                                         cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                              >  2:1493328/1‑101 (MQ=255)
                                                                         cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                              <  1:3640770/101‑1 (MQ=255)
                                                                         cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAccg                              <  2:2949293/101‑1 (MQ=255)
                                                                          gggATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAgcgc                             >  1:2225443/1‑101 (MQ=255)
                                                                            gATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                           >  2:2771542/1‑101 (MQ=255)
                                                                            gATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                           >  2:1874104/1‑101 (MQ=255)
                                                                              tatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                         >  1:3683254/1‑101 (MQ=255)
                                                                              tatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                         <  2:3763654/101‑1 (MQ=255)
                                                                               atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                        <  1:1560879/101‑1 (MQ=255)
                                                                               atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                        >  2:4278323/1‑101 (MQ=255)
                                                                               atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                        >  1:1211740/1‑101 (MQ=255)
                                                                                  tGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATc                     >  1:3869792/1‑101 (MQ=255)
                                                                                  tGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATc                     >  1:3586026/1‑101 (MQ=255)
                                                                                   gAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcc                    >  1:2033703/1‑101 (MQ=255)
                                                                                    aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc                   >  1:3760642/1‑101 (MQ=255)
                                                                                     ggCAAGCTGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc                  <  1:736439/101‑1 (MQ=255)
                                                                                     ggCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc                  >  2:1302902/1‑101 (MQ=255)
                                                                                      gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                 >  1:911118/1‑101 (MQ=255)
                                                                                       cAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgc                >  2:1616172/1‑101 (MQ=255)
                                                                                         aGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcgg              <  1:3967083/101‑1 (MQ=255)
                                                                                             gAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg          >  1:1472402/1‑101 (MQ=255)
                                                                                               aaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc        <  1:1050148/101‑1 (MQ=255)
                                                                                               aaGCCGATCCGGGCTTCGNTTCATCTTGCCCCAACTATCCCGGGGGTN.AATTTTGGCGCAGCCAAAAGAGNNNNNCGCCCNCCCTCNNCCCcncgcgcggc        >  1:2891020/1‑101 (MQ=21)
                                                                                                  ccGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggc     <  2:863435/101‑1 (MQ=255)
                                                                                                  ccGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggc     <  2:1123079/101‑1 (MQ=255)
                                                                                                    gATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgc   >  1:2813568/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
CGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.C.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTG.AATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCG  >  NC_014034/432369‑432568

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG