Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 568,802 Δ1 bp 7.0% intergenic (‑98/+68) RCAP_rcc00530 ← / ← carB hypothetical protein/carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034568,8020C→. 7.0% 28.2 ‑0.6 ‑0.7158intergenic (‑98/+68)RCAP_rcc00530/carBhypothetical protein/carbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (6/5):  ref base (94/53):  total (100/58)
Fisher's exact test strand distribution p-value = 5.34e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.09e-01

Only 100 of 209 total aligned reads displayed.

CATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGCC  >  NC_014034/568702‑568902
                                                                                                     |                                                                                                       
 aTTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cc                                                                                                        >  1:3324516/1‑101 (MQ=255)
  ttCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.ccc                                                                                                       <  2:183521/101‑1 (MQ=255)
  ttCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCGG.cca                                                                                                       >  1:4284468/1‑100 (MQ=255)
   tCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cccc                                                                                                      >  1:4395034/1‑101 (MQ=255)
   tCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cccc                                                                                                      <  2:3376824/101‑1 (MQ=255)
   tCCTGCATCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGAGCTGCGGCATCCTG.cccc                                                                                                      >  2:2582630/1‑101 (MQ=255)
    ccTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.ccccc                                                                                                     >  1:864313/1‑101 (MQ=255)
    ccTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCCGCATCCTG.ccccc                                                                                                     >  1:906836/1‑101 (MQ=255)
       gCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.ccccccc.c                                                                                                 <  1:887067/101‑1 (MQ=255)
         cTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.ccccccc.ccc                                                                                               <  1:2158906/101‑1 (MQ=255)
          tCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGCCCCCCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCg                                                                                              <  1:2582801/101‑1 (MQ=255)
          tCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCg                                                                                              >  1:3495684/1‑101 (MQ=255)
            cggTTTCGGTAGGGGAACCGGGGCTTACGCCGGTGGCGGGGGGGGGGCAATGACCCGCAGGGGTTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAc                                                                                            <  2:1107040/99‑1 (MQ=255)
                 tCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGag                                                                                      >  2:2610708/1‑101 (MQ=255)
                  cTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGCC.GGGagg                                                                                     >  1:2629361/1‑101 (MQ=255)
                  cTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGagg                                                                                     <  2:295424/101‑1 (MQ=255)
                   tGTATGTGATCCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGTTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGaggg                                                                                    <  2:3449288/101‑1 (MQ=255)
                   tGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCCAC.GGGgggg                                                                                    >  1:4049106/1‑101 (MQ=255)
                    gTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGggggg                                                                                   >  1:295459/1‑100 (MQ=255)
                    gTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGagggc                                                                                   <  2:1750409/101‑1 (MQ=255)
                    gTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGagggc                                                                                   >  2:2498241/1‑101 (MQ=255)
                      aTGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGagggca.g                                                                                >  2:2517825/1‑101 (MQ=255)
                      aTGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG..CCCCCC.CCCGAC.GGGagggca.gg                                                                               >  1:729841/1‑101 (MQ=255)
                       tgtgAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGagggca.gg                                                                               <  2:4379277/101‑1 (MQ=255)
                         tgAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCCAC.GGGggggca.gggc                                                                             >  1:3761810/1‑101 (MQ=255)
                          gAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCCAC.GGGGGGGCC.GGGCt                                                                            >  2:897835/1‑101 (MQ=255)
                           aaCCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTg                                                                           <  1:2595726/101‑1 (MQ=255)
                           aaCCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTg                                                                           <  2:3324284/101‑1 (MQ=255)
                            aCCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTgg                                                                          >  1:631207/1‑101 (MQ=255)
                             ccGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGt                                                                         <  2:2633815/101‑1 (MQ=255)
                              cGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCCAC.GGGAGGGCG.GGGCTGGtt                                                                        >  2:1333305/1‑101 (MQ=255)
                               ggggCTTACGCCTGGGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGttt                                                                       <  1:3244149/101‑1 (MQ=255)
                                gggCTTACGCCTGTGGCTGCGGTGGGGCAATGCCCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTc                                                                      <  1:316239/101‑1 (MQ=255)
                                 ggCTTACGCCGGGGGCTGCGGTGGGGCAATGCCCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCg                                                                     <  1:1296394/101‑1 (MQ=255)
                                   cTTACGCCTGTGGCTGCGGTGGGGCAATGGCCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCCGC.GGGGGGGGC.GGGGTGGTTTTggg                                                                   >  2:3032459/1‑101 (MQ=255)
                                   cTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGGGGGCA.GGGCTGGTTTCggg                                                                   >  1:2124526/1‑101 (MQ=255)
                                     tACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCCAC.GGGGGGGCAGGGGCGGGTTTGgggg                                                                  >  2:3993962/1‑100 (MQ=255)
                                      aCGCCTTTGGAGTCGTTGGGTCGATGTCCCGATCGGGGTTGCGAAGGGGCTTTGTCATCCTG.CCCCCCC.CCCGAC.GGGAGTGCA.GGGCTTGTTTCGggcgg                                                                <  2:2063741/101‑1 (MQ=255)
                                      aCGACTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGGTTCGggcgg                                                                >  2:3773899/1‑101 (MQ=255)
                                        gCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGGGGGCA.GGGCTGGGTTCGggggggc                                                              >  2:2403236/1‑99 (MQ=255)
                                         ccTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCaa                                                             <  1:1802348/101‑1 (MQ=255)
                                         ccTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCaa                                                             >  2:1175886/1‑101 (MQ=255)
                                          cTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAg                                                            >  1:4132813/1‑101 (MQ=255)
                                          cTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGGTTCGGGCGGCAAg                                                            >  2:382021/1‑101 (MQ=255)
                                          cTGTGGCTGCGGGGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAg                                                            <  1:1697573/101‑1 (MQ=255)
                                           tgtgGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGGGGGCA.GGGCTGGTTTCGGGGGGGAAgg                                                           >  2:4188636/1‑101 (MQ=255)
                                           tgtgGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGGTTCGGGCGGCaaag                                                           >  2:2258625/1‑99 (MQ=255)
                                           tgtgGCTGCGGTGGGCCAAAAACCCGCACGGGATTGTGAAGGCGCTGCGGCGTCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAgg                                                           <  2:1004147/101‑1 (MQ=255)
                                           tgggggtGCGGTGGGGCAATGCCCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTGCCCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAg                                                            <  1:3155742/95‑1 (MQ=255)
                                            gtgGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCG.GGGCTGGTTTCGGGCGGCAAGgc                                                          <  1:1881979/101‑1 (MQ=255)
                                            gtgGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCCCCGGGGGGGGCG.GGGCGGGTTTCGGGGGGCAAgg                                                           >  1:971110/1‑101 (MQ=255)
                                            gtgGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCCAC.GGGAGGGCA.GGGCTGGGTTCGGGGGGCAAggg                                                          >  1:4103605/1‑100 (MQ=255)
                                               gCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG..CCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGccc                                                      <  2:320730/101‑1 (MQ=255)
                                                gtgggggggggCAATGCCCCGCCGGGGCTTGTGAAGGCGCGGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGccc                                                      <  1:1640363/94‑1 (MQ=255)
                                                cTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGccc                                                      >  1:2509866/1‑101 (MQ=255)
                                                 tGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGCC.GGGAGGGCA.GGGCTGGGTTCGGGCGGCAAGGCGcccc                                                     >  1:3193996/1‑100 (MQ=255)
                                                 ggCGGTGGGCCAATGGCCCGCGCGGGCTTGTGAAGGGGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCcg                                                     <  2:2162046/100‑1 (MQ=255)
                                                     gTGGGGCAATGCCCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCcgcggc                                                 <  1:656126/101‑1 (MQ=255)
                                                       ggggCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCCCC.CGGGGGGCC.GGGGCGGGTTCTGGGGGCCAGGGGCCcccgggcg                                               >  2:3622663/1‑98 (MQ=255)
                                                        gggCAATGACCCGCACGGGCTGGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCcgcggcgcg                                              <  1:1607399/101‑1 (MQ=255)
                                                         ggCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCcgcggcgcgg                                             <  2:1579283/101‑1 (MQ=255)
                                                         ggCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG..CCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCgcggcgcggc                                            >  2:1948888/1‑101 (MQ=255)
                                                          gcacgtccccGCAGGGGTTTTTGACGGCGCTGCTGCCTCCGC.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCgcggcgcggc                                            <  2:833950/94‑1 (MQ=255)
                                                            aaTGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCACCCGAC.GGGGGGGCG.GGGCTGGTTTCGGGGGGCAGGGCGCCCGCGGCGCGCcc                                           >  2:518569/1‑101 (MQ=255)
                                                             aTGCCCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGt                                         <  1:1925533/101‑1 (MQ=255)
                                                             aTGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCCAGGGGCCCGCGGCGCGGCCGt                                         >  2:335612/1‑101 (MQ=255)
                                                              tgccccGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG..CCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGc                                       <  1:1611602/98‑1 (MQ=255)
                                                              tGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCggg                                        >  1:3365984/1‑99 (MQ=255)
                                                               gACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTGCCCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCCCGGCGCGGGCggg                                        >  2:1791997/1‑99 (MQ=255)
                                                               gACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG..CCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGGTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCggggc                                      >  1:3024952/1‑97 (MQ=255)
                                                                    gccggggCTTGTGAAGGGGCTGCGGCCCCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCt                                  <  1:3078530/97‑1 (MQ=255)
                                                                    gCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCTGCt                                  >  2:3225148/1‑101 (MQ=255)
                                                                    gCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCCCGGCGCCGCCGTGCCTGCt                                  >  2:919691/1‑101 (MQ=255)
                                                                      gcGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTa                                <  2:646711/100‑1 (MQ=255)
                                                                         ggCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGGGCGGCCGCGCCCGCTCAccc                             >  1:1065320/1‑99 (MQ=255)
                                                                           cTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGACCGTGCCTGCTTACGCCt                           <  1:2517988/101‑1 (MQ=255)
                                                                            ttGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCCAC.GGGGGGGCA.GGGCTGGTTTCGGGCGGGAAGGGGCCCGCGGCGCGGCCGGGCCCGCTTACCcccc                          >  1:4197571/1‑99 (MQ=255)
                                                                             tgtgAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGc                         <  2:2818407/101‑1 (MQ=255)
                                                                              gtgAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGGGCCCGCGGCGCGGCCGGGCCTGCTTACGCCTGcc                        >  1:2051654/1‑100 (MQ=255)
                                                                              gtgAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCa                        <  2:456747/101‑1 (MQ=255)
                                                                              gtgAAGGCCCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCa                        <  1:3963871/101‑1 (MQ=255)
                                                                               tgAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCCAC.GGGAGGGCC.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGGCGGGCCTGCTTACGCCTGccg                       >  2:2879399/1‑99 (MQ=255)
                                                                               gggaGGGGCCGCGGCCCCCTT.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAg                       <  1:386497/98‑1 (MQ=255)
                                                                                 aaGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCTGCTTACGCCTGCAGCg                     >  1:2231840/1‑101 (MQ=255)
                                                                                  aGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCgg                    <  2:631139/101‑1 (MQ=255)
                                                                                   ggCGCGGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGc                   <  1:2750486/101‑1 (MQ=255)
                                                                                    gcgcGGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGcc                  <  1:1818404/101‑1 (MQ=255)
                                                                                     cggggCGGCCTCCCG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCg                 <  2:1501784/97‑1 (MQ=255)
                                                                                     cgcTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCC.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCTGCTTACGCCTGCCGCGGCCg                 >  1:1330278/1‑101 (MQ=255)
                                                                                     cccTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCg                 <  2:1896866/99‑1 (MQ=255)
                                                                                      gcTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGGC.GGGCTGGTTTCGGGGGGGAAGGCGCCCGGGGGGCGGCCCGGCCCGCTTACGCCTGCAGCGGGCgg                >  2:1082275/1‑101 (MQ=255)
                                                                                       cTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCCCAGCCGTGCCTGCTTACCCCTGCAGCgggccgg               >  2:3580742/1‑96 (MQ=255)
                                                                                          cGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCg            <  2:4215020/101‑1 (MQ=255)
                                                                                           ggCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCga           >  1:3667713/1‑101 (MQ=255)
                                                                                           gccttCCGG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCCCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCga           <  1:382060/97‑1 (MQ=255)
                                                                                            gCATCCTC.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCgag          <  1:3010687/101‑1 (MQ=255)
                                                                                                ccgg.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGAttt      <  1:2722320/98‑1 (MQ=255)
                                                                                                ccTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGCCCCAGAgtt      >  2:2102291/1‑101 (MQ=255)
                                                                                                ccTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGAttt      <  2:2745155/101‑1 (MQ=255)
                                                                                                ccTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCTGCTTACGCCTGCAGCGGCCGGGCCGAGGttt      >  2:310019/1‑101 (MQ=255)
                                                                                                     |                                                                                                       
CATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCC.CCCGAC.GGGAGGGCA.GGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGCC  >  NC_014034/568702‑568902

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG